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Tea leaf’s microstructure and ultrastructure response to low temperature in indicating critical damage temperature

Information Processing in Agriculture 2019年第2期6卷 247-254页

作者： Yongzong Lu Yongguang Hu Richard LSnyder eric rkent Key Laboratory of Modern Agricultural Equipment and Technology Ministry of Education Jiangsu ProvinceJiangsu UniversityZhenjiangJiangsu 212013China Department of Land Air and Water ResourcesUniversity of CaliforniaDavisDavis 95616USA Department of Plant Sciences University of CaliforniaDavisDavis 95616USA

To find out the critical damage temperature of tea leaf,a new method of subzero treatment was provided by fitting the air temperature data from six heavy frost ***,the study explored the characteristics of Fuding Dabai tea plant response to low temperature stress of 2,0,2,4,8,10 and15C by observing the microstructure and ultrastructure changes of the *** samples were collected in an ambient temperature of 8.6C which served as *** electron microscopy(SEM)and transmission electron microscopy(TEM)were used to observe the microstructure and ultrastructure of stomata and *** observation results indicated that stomata of tea leaves have an obvious low temperature stress when the temperature was lower than2*** extent of opening of the stomata increased and enhanced guard cell protection of tea leaves against cold ***,dehydration,shrinkage and deformation of cells occurred as the temperature decreased from2C to15*** observations showed that the cell nucleus,cell walls,chloroplasts and mitochondria all had normal structure at a temperature of 8.6C where the membrane and granum lamella were clearly *** the temperature decreased to2C,the membrane system of tea leaf was the first to be damaged and the cell walls became ***,the leaf microstructure and ultrastructure showed obvious changes at2C,which might define the critical damage temperature for freeze damage of Fuding Dabai *** strategy based this critical damage temperature is useful for wind machine frost protection in tea fields within the Yangtze River region.

关键词： Frost damage Freeze injury Chill injury Frost protection

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