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Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

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World Journal of Gastroenterology 2025年第1期31卷 126-145页

作者： Xing-Hui Yu Yan Xie Jian Yu Kun-Ning Zhang Zhou-Bo Guo Di Wang Zhao-Xian Li Wei-qi Zhang Yu-Ying Tan Li Zhang Wen-Tao Jiang School of Medicine Nankai UniversityTianjin 300192China Tianjin Key Laboratory of Molecular Diagnosis and Treatment of Liver Cancer Tianjin First Center HospitalTianjin 300192China Department of Liver Transplantation Tianjin First Center HospitalTianjin 300192China First Central Clinical School Tianjin Medical UniversityTianjin 300192China

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated *** therapeutic targets are beneficial to treat and predict the ***,the exact molecular mechanisms leading to the progression of HCC are still *** has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor *** this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between *** To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC *** In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC ***,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,*** statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank *** In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival ***1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated ***,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via *** the exploration mechanism,we found ASH1L may promote an immunosuppressive mi

关键词： Hepatocellular carcinoma MicroRNA-142-3p ASH1L Immune evasion Tumor immune microenvironment Apoptosis

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Anlotinib-induced sick sinus syndrome:Two case reports

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World Journal of Clinical Cases 2025年第6期13卷 30-34页

作者： Cheng-Feng Fu Li-Fen Yang Lei Tian Song Deng qi Zhang Biao Yao Department of Oncology Tongren People’s HospitalTongren 554300Guizhou ProvinceChina Department of Oncology Guizhou Provincial People’s HospitalGuiyang 550000Guizhou ProvinceChina

BACKGROUND This manuscript describes the first known cases of sick sinus syndrome(SSS)associated with the use of anlotinib in non-small cell lung cancer patients,highlighting the need for increased vigilance and cardiac *** SUMMARY Two patients with non-small cell lung cancer developed SSS after 15 months and 5 months of anlotinib treatment,respectively,presenting with syncope and *** confirmed SSS,and different treatment approaches were taken for each *** patient received a dual-chamber permanent pacemaker,while the other discontinued the medication and experienced symptom *** Anlotinib can induce SSS,suggesting that cardiac monitoring is crucial during anlotinib *** management strategies are necessary for affected individuals.

关键词： Lung cancer Anlotinib Sick sinus syndrome Cardiac pacemaker Adverse drug reaction Case report

Pulp health and calcific healing of a complicated crown–root fracture with additional root fracture in a maxillary incisor: A case report

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World Journal of Clinical Cases 2025年第3期13卷 42-49页

作者： Na Li Yue-Yue Ren Ying Tang qi Yang Tian-Tian Meng Song Li Jing Zhang Stomatological Hospital and College Anhui Medical UniversityHefei 230032Anhui ProvinceChina Department of Endodontics The Affiliated Stomatological Hospital of Xuzhou Medical UniversityXuzhou 221000Jiangsu ProvinceChina

BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in *** SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical *** the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival ***-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival *** This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture.

关键词： Complicated crown-root fracture Multiple root fracture Spontaneous healing Cone-beam computed tomography Long-term follow-up Case report

Fe_(n)Mo_(38-n)(n=0-38)及Fe_(n)Mo_(55-n)(n=0-55)双金属团簇的结构演化和基态能量

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原子与分子物理学报 2025年第3期42卷 85-93页

作者：郑琪琪陈轩程彪段海明新疆大学物理科学与技术学院乌鲁木齐830017

基于半经验的Gupta多体势,采用遗传算法及分子动力学淬火算法,系统研究了(FeMo)m(m=38及55)双金属团簇的基态结构及其能量.结果表明:对于Fe_(n)Mo_(38-n)(n=0-38)团簇,随Fe原子数的增加,Fe原子优先占据团簇表面再占据内部,其基态构型存... 详细信息

基于半经验的Gupta多体势,采用遗传算法及分子动力学淬火算法,系统研究了(FeMo)m(m=38及55)双金属团簇的基态结构及其能量.结果表明:对于Fe_(n)Mo_(38-n)(n=0-38)团簇,随Fe原子数的增加,Fe原子优先占据团簇表面再占据内部,其基态构型存在类O_(h)结构、类I_(h)结构和无序结构间的竞争.对于Fe_(n)Mo_(55-n)(n=0-55)团簇,随Fe原子数的增加,Fe原子优先占据团簇中心位置,再依次占据表面顶点、棱边和次外层,双金属团簇基态构型主要体现为在Mackay二十面体基础上的结构畸变.Fe_(24)Mo_(14),Fe_(13)Mo_(42)和Fe_(43)Mo_(12)为幻数结构团簇,研究发现双金属团簇幻数成因不能通过单质团簇常用的平均配位数和平均键长模型解释,它更多的归咎于组分效应导致的结构高对称性.

关键词：双金属团簇结构和能量淬火算法遗传算法

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Fe_(n)Co_(55-n)与Co_(n)Mo_(55-n)(n=0~55)团簇基态的能量与结构演化

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原子与分子物理学报 2025年第5期42卷 57-66页

作者：程彪郑琪琪陈轩段海明新疆大学物理科学与技术学院乌鲁木齐830049

采用半经验的Gupta多体势函数结合遗传算法及分子动力学淬火算法,系统研究了Co_(n)Mo_(55-n)(n=0~55)与Fe_(n)Co_(55-n)(n=0~55)团簇的基态结构与能量.通过分析基态结构的相似函数、质心间距、平均键长、平均原子配位数、平均结合能、... 详细信息

采用半经验的Gupta多体势函数结合遗传算法及分子动力学淬火算法,系统研究了Co_(n)Mo_(55-n)(n=0~55)与Fe_(n)Co_(55-n)(n=0~55)团簇的基态结构与能量.通过分析基态结构的相似函数、质心间距、平均键长、平均原子配位数、平均结合能、混合能与二阶差分能.探究了团簇基态的结构特征及稳定性与团簇所含元素种类及组分的关联.结果表明:对Co-Mo混合团簇,随Co原子数目增加,Co原子依次占据团簇中心与最外层顶点,混合团簇逐渐出现组分偏析与无序现象;对Fe-Co混合团簇,基态结构主体上均体现为围绕Mackay二十面体构型的结构畸变,随Fe原子含量增加,Fe原子先占据团簇外表面顶点位置直到全部包裹Co原子,逐渐形成核壳式结构;Co_(13)Mo_(42)与Fe_(42)Co_(13)是幻数结构团簇,研究发现组分效应导致的团簇高对称性相比团簇平均键长与平均原子配位数更能解释幻数结构的成因.

关键词：遗传算法淬火算法双金属团簇基态结构

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Impacts of Nutlin-3a and exercise on murine double minute 2-enriched glioma treatment

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Neural Regeneration Research 2025年第4期20卷 1135-1152页

作者： Yisheng Chen Zhongcheng Fan Zhiwen Luo Xueran Kang Renwen Wan Fangqi Li Weiwei Lin Zhihua Han Beijie qi Jinrong Lin Yaying Sun Jiebin Huang Yuzhen Xu Shiyi Chen Department of Sport Medicine Huashan HospitalFudan UniversityShanghaiChina Department of Orthopedic Surgery Hainan Province Clinical Medical CenterHaikou Affiliated Hospital of Central South University Xiangya School of MedicineHaikouHainan ProvinceChina Department of Basic Medicine Shanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Neurosurgery Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Department of Orthopedics Shanghai General HospitalSchool of Medicine Shanghai Jiao Tong UniversityShanghaiChina Department of Infectious Diseases Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina

Recent research has demonstrated the impact of physical activity on the prognosis of glioma patients,with evidence suggesting exercise may reduce mortality risks and aid neural *** role of the small ubiquitin-like modifier(SUMO)protein,especially post-exercise,in cancer progression,is gaining attention,as are the potential anti-cancer effects of *** used machine learning to create the exercise and SUMO-related gene signature(ESLRS).This signature shows how physical activity might help improve the outlook for low-grade glioma and other *** demonstrated the prognostic and immunotherapeutic significance of ESLRS markers,specifically highlighting how murine double minute 2(MDM2),a component of the ESLRS,can be targeted by *** underscores the intricate relationship between natural compounds such as nutlin-3 and immune *** comprehensive CRISPR screening,we validated the effects of specific ESLRS genes on low-grade glioma *** also revealed insights into the effectiveness of Nutlin-3a as a potent MDM2 inhibitor through molecular docking and dynamic ***-3a inhibited glioma cell proliferation and activated the p53 *** efficacy decreased with MDM2 overexpression,and this was reversed by Nutlin-3a or *** using a low-grade glioma mouse model highlighted the effect of physical activity on oxidative stress and molecular pathway ***,both physical exercise and Nutlin-3a administration improved physical function in mice bearing tumors derived from MDM2-overexpressing *** results suggest the potential for Nutlin-3a,an MDM2 inhibitor,with physical exercise as a therapeutic approach for glioma *** research also supports the use of natural products for therapy and sheds light on the interaction of exercise,natural products,and immune regulation in cancer treatment.

关键词： exercise and SUMO-related gene signatures(ESLRS) glioblastoma management low-grade glioma natural bioactives neural regeneration physical exercise

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Asymptotic false discovery control of the Benjamini-Hochberg procedure for pairwise comparisons

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Science China Mathematics 2025年第1期 223-252页

作者： Weidong Liu Dennis Leung qi-Man Shao School of Mathematical Sciences Shanghai Jiao Tong University School of Mathematics and Statistics University of Melbourne Department of Statistics and Data Science SICMSouthern University of Science and Technology

In a one-way analysis-of-variance(ANOVA) model,the number of pairwise comparisons can become large even with a moderate number of *** by this,we consider a regime with a growing number of groups and prove that,when testing pairwise comparisons,the Benjamini-Hochberg(BH) procedure can asymptotically control false discoveries,despite the fact that the involved t-statistics do not exhibit the wellknown positive dependence structure required for exact false discovery rate(FDR) *** Tukey's perspective that the difference between the means of any two groups cannot be exactly zero,our main result provides control over the directional false discovery rate and directional false discovery proportion.A key technical contribution of our work is demonstrating that the dependence among the t-statistics is sufficiently weak to establish the convergence result typically required for asymptotic FDR *** analysis does not rely on conventional assumptions such as normality,variance homogeneity,or a balanced design,thereby offering a theoretical foundation for applications in more general settings.

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生成式人工智能应用场景下个人信息保护的法律风险及应对

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太原学院学报（社会科学版） 2025年第1期26卷 58-70页

作者：张淇山西财经大学法学院山西太原030006

数据已成为新的产权要素和产业基础,而具备了一定理性能力的生成式人工智能ChatGPT在大数据喂养、人为数据标识处理和信息再输出三个层次对个人信息权益构成了新的侵权范式。尽管《个人信息保护法》《网络安全法》等法律规范已确立起一... 详细信息

数据已成为新的产权要素和产业基础,而具备了一定理性能力的生成式人工智能ChatGPT在大数据喂养、人为数据标识处理和信息再输出三个层次对个人信息权益构成了新的侵权范式。尽管《个人信息保护法》《网络安全法》等法律规范已确立起一系列个人信息保护手段,但在生成式人工智能服务与应用端仍面临着解释和适用上的难题。为此,我国需要在保障个人信息权益的前提下鼓励发展人工智能技术——在总体上要树立起基于风险的个人信息保护原则,并尝试以“风险预知—解构—化解”为基本路径,在微观上优化“知情-同意”规则,以“设计保护”原则强化企业内部自治,并灵活调整侵权责任追究机制。

关键词：《个人信息保护法》 ChatGPT 生成式人工智能风险控制