检索结果-南通市图书馆

DNAH17-AS1 promotes pancreatic carcinoma by increasing PPME1 expression via inhibition of miR-432-5p

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第15期26卷 1745-1757页

作者： Tao Xu Ting Lei si-Qiao Li er-Hui Mai Fei-Hu Ding Bin Niu Department of Hepatobiliary Surgery Luoyang Central Hospital Affiliated to Zhengzhou UniversityLuoyang 471000Henan ProvinceChina Department of Gastrointestinal Surgery The Fifth Affiliated Hospital of Sun Yat-Sen UniversityZhuhai 519000Guangdong ProvinceChina

BAcKGRoUND The incidence and mortality rates of pancreatic carcinoma(Pc)are rapidly increasing *** noncoding RNAs(lncRNAs)play critical roles during Pc initiation and *** the lncRNA DNAH17-AS1 is highly expressed in Pc,the regulation of DNAH17-AS1 in Pc was investigated in this *** To investigate the expression and molecular action of lncRNA DNAH17-AS1 in Pc *** The Pc expression data for the lncRNA DNAH17-AS1 was downloaded from The cancer Genome Atlas database and used to examine its *** blot and reverse transcription-quantitative PcR were employed to assess protein and mRNA expression.A subcellular fractionation assay was used to determine the location of DNAH17-AS1 in *** addition,the regulatory effects of DNAH17-AS1 on miR-432-5p,PPME1,and tumor activity were investigated using luciferase reporter assay,MTT viability analysis,flow cytometry,and transwell migration *** DNAH17-AS1 was upregulated in Pc cells and was associated with aggressive tumor behavior and poor prognosis for *** DNAH17-AS1 promoted the apoptosis and reduced the viability,invasion,and migration of Pc *** addition,DNAH17-AS1 served as a Pc oncogene by downregulating miR-432-5p which normally directly targeted PPME1 to downregulate its *** DNAH17-AS1 functions in Pc as a tumor promoter by regulating the miR-432-5p/PPME1 *** finding may provide new insights for Pc prognosis and therapy.

关键词： Long noncoding RNAs DNAH17-AS1 Pancreatic carcinoma MiR-432-5p PPME1 Molecular mechanism

盐生草根系基因HgAKR6c的克隆与初步功能分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

草业学报 2024年第1期33卷 61-74页

作者：胡尚钦汪军成姚立蓉司二静马小乐杨轲张宏孟亚雄王化俊李葆春甘肃农业大学干旱生境作物学国家重点实验室甘肃省作物遗传改良与种质创新重点实验室甘肃兰州730070 甘肃农业大学生命科学技术学院甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070

醛酮还原酶(AKR)是构成Shaker型K+通道蛋白的保守核心结构域,在植物应对非生物胁迫时起到关键作用。本研究采用西北旱区典型盐生植物盐生草作为研究材料,基于课题组前期盐生草根系盐胁迫转录组学数据分析结果,筛选并克隆得到耐盐基因HgA... 详细信息

醛酮还原酶(AKR)是构成Shaker型K+通道蛋白的保守核心结构域,在植物应对非生物胁迫时起到关键作用。本研究采用西北旱区典型盐生植物盐生草作为研究材料,基于课题组前期盐生草根系盐胁迫转录组学数据分析结果,筛选并克隆得到耐盐基因HgAKR6c。HgAKR6c基因蛋白质编码区(cDS)全长951 bp,共编码氨基酸317个。系统发育进化树分析表明,HgAKR6c与拟南芥中AtAKR6c1基因亲缘关系最近。亚细胞定位表明该基因可能主要定位于细胞质和细胞核中。qRT-PcR结果表明HgAKR6c在盐处理24 h时表达量达到峰值。构建酵母异源表达载体转化缺陷型菌株发现,HgAKR6c基因可能参与Na+的外排和介导K+的吸收。综上所述,HgAKR6c具有调节盐生草耐盐性的功能,而盐生草根系耐盐基因HgAKR6c的调控机制还需进一步的研究验证。

关键词：盐生草 HgAKR6c 基因克隆耐盐调控机制

盐生草AKR基因家族成员的鉴定及根系盐胁迫响应基因HgAKR42639的耐盐分析

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

草业学报 2024年第7期33卷 68-83页

作者：张震欢姚立蓉汪军成司二静张宏杨轲马小乐孟亚雄王化俊李葆春甘肃农业大学省部共建干旱生境作物学国家重点实验室甘肃省作物遗传改良与种质创新重点实验室甘肃兰州730070 甘肃农业大学生命科学技术学院甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070

为探究盐生草AKR基因家族的生物学功能和根系盐胁迫响应基因HgAKR42639的耐盐性,基于盐生草全长转录组测序鉴定醛酮还原酶基因(AKRs),采用生物信息学方法分析盐生草AKRs编码的蛋白质序列特性,并在200 mmol·L^(-1)Nacl胁迫0、6、12、24... 详细信息

为探究盐生草AKR基因家族的生物学功能和根系盐胁迫响应基因HgAKR42639的耐盐性,基于盐生草全长转录组测序鉴定醛酮还原酶基因(AKRs),采用生物信息学方法分析盐生草AKRs编码的蛋白质序列特性,并在200 mmol·L^(-1)Nacl胁迫0、6、12、24和48 h处理下,测定盐生草幼苗根系和转HgAKR42639基因拟南芥的目的基因表达量、生理指标和钠钾离子含量。结果表明:从盐生草转录组中鉴定出23个HgAKRs,编码的氨基酸数量在165~664 aa,亚细胞定位预测主要在细胞质中,AKR蛋白保守结构域高度相似,具有3个motif,启动子顺式作用元件分析存在核心元件、增强元件和胁迫响应元件;qRT-PcR结果显示HgAKR42639基因相对表达量在盐生草根系和拟南芥中均先上升后下降,24 h两者的表达量达到峰值;随着盐胁迫时间的增长两者的过氧化氢酶(cAT)、过氧化物酶(PoD)、超氧化物歧化酶(SoD)活性和脯氨酸含量呈上升趋势,丙二醛(MDA)和可溶性蛋白含量呈下降趋势,在24 h达到最低;Na^(+)和K^(+)含量测定结果显示,两者于24 h盐胁迫处理下Na^(+)含量下降,K^(+)/Na^(+)达到最大值。综上所述,本研究获得了盐生草AKR家族基因,为后续研究盐生草AKR基因响应盐胁迫的分子机制提供理论依据;以期为进一步验证HgAKR42639基因的耐盐性奠定基础。

关键词：盐生草 AKR基因家族生物信息学分析盐胁迫

Manifestation of the malignant progression of glioma following initial intracerebral hemorrhage:A case report

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

World Journal of clinical cases 2023年第7期11卷 1576-1585页

作者： En-Xi Xu si-Yuan Lu Bo chen Xiao-Dong Ma er-Yi Sun Department of Neurosurgery The Affiliated People’s Hospital of Jiangsu UniversityZhenjiang 212002Jiangsu ProvinceChina Department of Radiology The Affiliated People’s Hospital of Jiangsu UniversityZhenjiang 212002Jiangsu ProvinceChina Department of Anesthesia The Affiliated People’s HospitalZhenjiang 212002Jiangsu ProvinceChina

BAcKGRoUND Intracranial hemorrhage is extremely rare during the initial stages of ***,we report a case of glioma with unclassified pathology and intracranial *** SUMMARY After the second surgery for intracerebral hemorrhage,the patient experienced weakness in the left arm and leg,but could walk *** month after discharge,the weakness in the left limbs had exacerbated and the patient also suffered from headaches and dizziness.A third surgery was ineffective against the rapidly growing *** hemorrhage may be the initial symptom of glioma in some rare cases,and atypical perihematomal edema can be used for diagnosis during an *** histological and molecular features seen in our case were similar to that of glioblastoma with a primitive neuronal component,which is termed diffuse glioneuronal tumor with features similar to oligodendroglioma and nuclear clusters(DGoNc).The patient underwent three surgeries to remove the *** first tumor resection had been per.ormed when the patient was *** of the hemorrhage and bone disc decompression were per.ormed when the patient was *** month after the last discharge,the patient underwent neuronavigation-assisted resection of the right frontotemporal parietal lesion plus extended flap *** the 50^(th)d after the third operation,computed tomography imaging showed rapid tumor growth accompanied by brain *** patient was discharged and died 3 d *** Glioma can present as bleeding in the initial stage and should be considered in such a *** have reported a case of DGoNc,which is a rare molecular subtype of glioma with a unique methylation profile.

关键词： Intracranial hemorrhage Glioma Glioneuronal tumor Rare case report

siRNA therapeutics:a clinical reality

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science china(Life Sciences) 2020年第4期63卷 485-500页

作者： Phei er Saw er-Wei Song Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Breast Tumor center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Zhongshan School of Medicine Breast SurgeryGuangzhou 510080China

since the revolutionary discovery of RNA interference(RNAi),a remarkable progress has been achieved in understanding and harnessing gene silencing mechanism;especially in small interfering RNA(siRNA)*** its tremendous potential benefits,major challenges in most siRNA therapeutics remains unchanged-safe,efficient and target oriented delivery of *** years after the discovery of RNAi,siRNA therapeutics finally charts its way into *** we journey through the decades,we reminisce the history of siRNA discovery and its application in a myriad of disease ***,we highlight the breakthroughs in si RNA therapeutics,with special feature on the first FDA approved RNAi therapeutics onpattro(Patisiran)and the consideration of effective siRNA delivery system focusing on current siRNA nanocarrier in clinical ***,we present some challenges and multiple barriers that are yet to be fully over.ome in siRNA therapeutics.

关键词： siRNA therapeutic disease treatment systemic siRNA delivery clinical application

大麦籽粒β-葡聚糖含量的全基因组关联分析及候选基因预测

在线全文

学校读者我要写书评

暂无评论

作物学报 2024年第10期50卷 2483-2492页

作者：鲁宗辉司二静叶霈颖汪军成姚立蓉马小乐李葆春王化俊尚勋武孟亚雄省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070 甘肃农业大学生命科学技术学院甘肃兰州730070

利用高通量SNP芯片对238份不同来源的大麦种质资源进行基因型分析,并测定2年的籽粒β-葡聚糖含量,通过基于PcA模型的一般线性模型(general linear model,GLM)进行全基因组关联分析(genome-wide association study,GWAS)。结果表明,238... 详细信息

利用高通量SNP芯片对238份不同来源的大麦种质资源进行基因型分析,并测定2年的籽粒β-葡聚糖含量,通过基于PcA模型的一般线性模型(general linear model,GLM)进行全基因组关联分析(genome-wide association study,GWAS)。结果表明,238份大麦材料的β-葡聚糖含量分布在1.23%~6.55%和1.79%~6.64%之间且均呈正态分布。GWAS分析共检测到19个显著的SNP标记,分布在1H、2H、3H、4H和5H染色体上,可解释表型变异的7.39%~10.29%。在显著关联的SNP位点上下游各300 kb范围内进行候选基因挖掘,共寻找到37个基因,基于前人研究和BLAST基因注释共筛选到4个最有可能与β-葡聚糖合成相关的候选基因,在最显著SNP位点B1_1033963上游89 kb处找到候选基因***.r3.1HG0000140,该基因可能是与β-葡聚糖合成过程紧密相关的基因。本研究可为大麦β-葡聚糖含量遗传改良提供理论指导与优异基因资源。

关键词：大麦 β-葡聚糖全基因组关联分析 SNP标记

维普期刊数据库博看期刊

基于低场核磁共振技术的玉米子粒发育过程中水分变化规律

在线全文

学校读者我要写书评

暂无评论

玉米科学 2024年第2期32卷 69-76页

作者：段思菲陈怡许莞儿吕函默唐旺苏金龙殷诗凯陈琪董学会中国农业大学农学院北京100193

基于低场核磁共振(low-field nuclear magnetic resonance,LF-NMR)技术观测子粒发育过程的水分迁移动态,分析不同种质玉米子粒水分变化规律。结果表明,在子粒发育初期,郑单958与先玉335的子粒含水率存在显著差异,且一直伴随整个发育时... 详细信息

基于低场核磁共振(low-field nuclear magnetic resonance,LF-NMR)技术观测子粒发育过程的水分迁移动态,分析不同种质玉米子粒水分变化规律。结果表明,在子粒发育初期,郑单958与先玉335的子粒含水率存在显著差异,且一直伴随整个发育时期。在整个发育期,自由水和半结合水含量逐渐降低,结合水含量逐渐升高,整体水分向结合水方向迁移。DAP36-DAP43是两品种水分相态差异最大的生育期;在成熟期,半结合水是两品种间唯一有差异的水分相态。各指标相关性分析结果显示,淀粉与结合水呈显著正相关,与自由水、半结合水呈显著负相关。综上,LF-NMR可以快速且无损检测玉米子粒发育过程的水分变化。在玉米子粒发育过程中,子粒含水率不断降低的同时伴随着水分向自由度降低方向转化,淀粉积累可能是促使这一过程的因素之一,半结合水可能是影响成熟后期两个品种脱水差异的水分相态。

关键词：玉米水分迁移水分相态 LF-NMR

Photoionisation detection of a single er3+ ion with sub-100-ns time resolution

在线全文

学校读者我要写书评

暂无评论

National Science Review 2024年第4期11卷 38-45页

作者： Yangbo Zhang Wenda Fan Jiliang Yang Hao Guan Qi Zhang Xi Qin changkui Duan Gabriele G.de Boo Brett c.Johnson Jeffrey c.Mc callum Matthew J.Sellars Sven Rogge chunming Yin Jiangfeng Du cAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences University of Science and Technology of China cAS center for Excellence in Quantum Information and Quantum Physics University of Science and Technology of China Hefei National Laboratory University of Science and Technology of China centre of Excellence for Quantum computation and communication Technology School of Physics University of New South Wales centre of Excellence for Quantum computation and communication Technology School of Engineering RMIT University centre of Excellence for Quantum computation and communication Technology School of Physics University of Melbourne centre of Excellence for Quantum computation and communication Technology Research School of Physics and Engineering Australian National University

Efficient detection of single optical centres in solids is essential for quantum information processing, sensing and single-photon generation *** this work,we use radio-frequency（RF） reflectometry to electrically detect the photoionisation induced by a single er3+ion in *** high bandwidth and sensitivity of the RF reflectometry provide sub-100-ns time resolution for the photoionisation *** this technique,the optically excited state lifetime of a single er3+ion in a si nano-transistor is measured for the first time to be 0.49±0.04μ*** results demonstrate an efficient approach for detecting a charge state change induced by er excitation and *** approach could be used for fast readout of other single optical centres in solids and is attractive for large-scale integrated optical quantum systems thanks to the multi-channel RF reflectometry demonstrated with frequency multiplexing techniques.

关键词： single optical centres erbium ions RF reflectometry photoionisation detection

槲皮素通过介导JNK信号通路抑制线粒体凋亡途径的神经保护作用

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国药理学通报 2024年第2期40卷 256-262页

作者：姚思凡张鑫戴月英沈丽霞河北北方学院药学院河北省神经药理学重点实验室河北张家口075000

目的研究槲皮素(quercetin,Que)通过调节JNK信号通路抑制Aβ25-35引起Pc12细胞线粒体损伤的作用机制。方法培养Pc12细胞,使用Aβ25-35诱导其构建阿尔茨海默病(Alzheimer′s disease,AD)受损模型,Que、17β-雌二醇(17β-E2)和金雀异黄素(... 详细信息

目的研究槲皮素(quercetin,Que)通过调节JNK信号通路抑制Aβ25-35引起Pc12细胞线粒体损伤的作用机制。方法培养Pc12细胞,使用Aβ25-35诱导其构建阿尔茨海默病(Alzheimer′s disease,AD)受损模型,Que、17β-雌二醇(17β-E2)和金雀异黄素(genistein,Gen)进行干预,并设定SP600125(JNK特异性抑制剂)预处理组。Fluo-4 AM染料荧光检测Pc12细胞钙离子;Rhodamine123染色液检测Pc12细胞线粒体膜电位;caspase-3活性检测caspase-3酶活性;免疫荧光检测p-JNK蛋白表达;Western blot法检测erα、erβ、p-JNK、JNK、Bcl-W、Bim和cytochrome c蛋白表达。并使用雌激素受体α/β拮抗剂MPP/PHTPP和SP600125进行干预,探讨Que发挥神经保护介导的分子机制。结果与Aβ25-35组相比,Aβ25-35+Que组能提高线粒体膜电位(Pcaspase-3检测和Western blot结果显示,与Aβ25-35组相比,Que可以逆转Aβ25-35诱导的erα表达降低(Pcaspase-3表达增加(Pcyt c的释放(Pcl-W的表达(Pcl-W、Bim和cyt c的作用和SP600125对Aβ25-35诱导Pc12细胞线粒体凋亡途径的保护作用机制相同(P<0.01)。结论Que可通过介导JNK信号通路抑制线粒体凋亡,进而发挥神经保护作用。

关键词：槲皮素阿尔茨海默病线粒体细胞凋亡 JNK信号通路神经保护作用