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Liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients

World Journal of Gastroenterology 2019年第29期25卷 3985-3995页

作者： Wen-Ying Xia Li Gao er-hei dai Dan Chen er-Fu Xie Li Yang Shi-Chang Zhang Bing-Feng Zhang Jian Xu Shi-Yang Pan Department of Laboratory Medicine the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China Department of Laboratory Medicine the Fifth Hospital of Shijiazhuang Shijiazhuang 050021 Hebei Province China

BACKGROUND Hepatitis B is a major public health problem in China. Accurate liver injury assessment is essential for clinical evidence-based treatment. Liver biopsy is considered the gold standard method to stage liver disease, but it is not widely used in resource-limited settings. Therefore, non-invasive liquid biopsy tests are needed. AIM To assess liver injury in hepatitis B patients using quantified cell free DNA combined with other serum biomarker as a liquid biopsy-based method. METHODS A cohort of 663 subjects including 313 hepatitis B patients and 350 healthy controls were enrolled. Ultrasound-guided liver biopsies followed by histopathological assessments were performed for the 263 chronic hepatitis B patients to determine the degree of liver injury. Cell-free DNA was quantified using a novel duplex real-time polymerase chain reaction assay. RESULTS Compared with healthy controls, patients with hepatitis B virus (HBV) infection had significantly higher plasma DNA, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, and HBV DNA levels (Perum ALT, AST, bilirubin, and plasma DNA levels of patients with markedsevere inflammation were significantly higher than those with mild-moderate inflammation (Pere was a statistically significant correlation between hepatocyte inflammation severity and serum bilirubin (R^2=0.673, Per the curves of serum ALT, bilirubin, plasma DNA, and their combination to distinguish between patients with mild–moderate and marked-severe inflammation were 0.8059, 0.7910, 0.7921, and 0.9564, respectively. CONCLUSION The combination of plasma DNA, serum ALT, and bilirubin could be a candidate liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients.

关键词： Liquid biopsy plasma DNA Hepatitis B Alanine aminotransferase Duplex real-time quantitative polymerase chain reaction

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Pregnancy and fetal outcomes of chronic hepatitis C mothers with viremia in China

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World Journal of Gastroenterology 2022年第34期28卷 5023-5035页

作者： Calvin Q Pan Bao-Shen Zhu Jian-Ping Xu Jian-Xia Li Li-Juan Sun Hong-Xia Tian Xi-Hong Zhang Su-Wen Li er-hei dai Center for Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Division of Gastroenterology and Hepatology Department of MedicineNYU Langone HealthNYU School of MedicineFlushingNY 11355United States Department of Obstetrics and Gynecology The Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuang 050021Hebei ProvinceChina School of Public Health North China University of Science and TechnologyTangshan 063210Hebei ProvinceChina Division of Liver Disease Department of MedicineThe Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuang 050021Hebei ProvinceChina

BACKGROUND Data that assess maternal and infant outcomes in hepatitis C virus(HCV)-infected mothers are *** To investigate the frequency of complications and the associated risk *** We performed a cohort study to compare pregnancy and fetal outcomes of HCVviremic mothers with those of healthy *** factors were analyzed with logistic *** Among 112 consecutive HCV antibody-positive mothers screened,we enrolled 79 viremic *** randomly selected 115 healthy mothers from the birth registry as the *** to healthy mothers,HCV mothers had a significantly higher frequency of anemia[2.6%(3/115)vs 19.0%(15/79),Per(3278.3±462.0 vs 3105.1±459.4 gms;P=0.006),and the mean head circumference was smaller(33.3±0.6 vs 33.1±0.7 cm;P=0.03).In a multivariate model,HCV-infected mothers were more likely to suffer anemia[adjusted odds ratio(OR):18.1,95%confidence interval(CI):4.3-76.6],require caesarian sections(adjusted OR:2.6,95%CI:1.4-4.9),and have nuchal cords(adjusted OR:5.6,95%CI:2.4-13.0).Their neonates were also more likely to have smaller head circumferences(adjusted OR:2.1,95%CI:1.1-4.3)and lower birth weights than the average(≤3250 gms)with an adjusted OR of 2.2(95%CI:1.2-4.0).The vertical transmission rate was 1%in HCV-infected *** Maternal HCV infections may associate with pregnancy and obstetric *** demonstrated a previously unreported association between maternal HCV viremia and a smaller neonatal head circumference,suggesting fetal growth restriction.

关键词： Hepatitis C virus viremia Mother-to-child transmission Pregnancy complications Maternal health Infant hepatitis C virus infection

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Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

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Signal Transduction and Targeted Therapy 2021年第10期6卷 3108-3118页

作者： Xiang-Na Zhao Yue You Xiao-Ming Cui Hui-Xia Gao Guo-Lin Wang Sheng-Bo Zhang Lin Yao Li-Jun Duan Ka-Li Zhu Yu-Ling Wang Li Li Jian-Hua Lu Hai-Bin Wang Jing-Fang Fan Huan-Wei Zheng er-hei dai Lu-Yi Tian Mai-Juan Ma Center for Disease Control and Prevention of Chinese People's Liberation Army BeijingChina The Walter and Eliza Hall Institute of Medical Research ParkvilleVicAustralia Department of Medical Biology The University of MelbourneParkvilleVicAustralia State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina The Fifth Hospital of Shijiazhuang Hebei Medical UniversityShijiazhuangChina

While some individuals infected by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)present mild-to-severe disease,many SARS-CoV-2-infected individuals are *** sought to identify the distinction of immune response between asymptomatic and moderate *** performed single-cell transcriptome and T-cell/B-cell receptor(TCR/BCR)sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic,moderate,and severe patients with healthy *** patients displayed increased CD56^(bri)CD16^(-) natural killer(NK)cells and upregulation of interferon-gamma in effector CD4^(+) and CD8^(+) T cells and NK *** showed more robust TCR clonal expansion,especially in effector CD4^(+) T cells,but lack strong BCR clonal expansion compared to moderate ***,asymptomatic patients have lower interferon-stimulated genes(ISGs)expression in general but large interpatient variability,whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe *** data provide evidenee of different immune signatures to SARS-CoV-2 in asymptomatic infections.

关键词： patients asymptomatic interferon

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HBsAg定量检测技术进展及其临床意义

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世界华人消化杂志 2017年第26期25卷 2322-2328页

作者：戴二黑李敏然石家庄市第五医院河北省石家庄市050021

近年来,乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)定量检测技术不断创新发展,目前在慢性乙型肝炎病毒(hepatitis B virus,HBV)感染患者中得到了广泛应用.血清HBsAg定量水平与HBV复制相关,有助于鉴别慢性HBV感染自然史及预... 详细信息

近年来,乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)定量检测技术不断创新发展,目前在慢性乙型肝炎病毒(hepatitis B virus,HBV)感染患者中得到了广泛应用.血清HBsAg定量水平与HBV复制相关,有助于鉴别慢性HBV感染自然史及预测疾病进展.实时监测HBsAg定量水平动态变化有助于预测慢性乙型肝炎(chronic hepatitis B,CHB)患者抗病毒治疗的疗效与指导停药,提高临床CHB患者优化治疗策略.现就目前HBsAg定量检测技术及其在临床应用方面的最新进展做一综述.

关键词：慢性乙型肝炎 HBsAg 技术治疗

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Quantitative characterizations of anisotropic dynamic properties in organic-rich shale with different kerogen content

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Petroleum Science 2024年第2期21卷 855-865页

作者： Jian-Yong Xie Yan-Ping Fang Xing-Hua Wu Jian'er Zhao Jun-Cheng dai Jun-Xing Cao Ji-Xin Deng State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of TechnologyChengdu610059SichuanChina Key Lab of Earth Exploration&Information Techniques of Ministry of Education Geophysical InstituteChengdu University of TechnologyChengdu610059SichuanChina PetroChina Southwest Oil&Gasfield Company Chengdu610017SichuanChina

Understanding the quantitative responses of anisotropic dynamic properties in organic-rich shale with different kerogen content(KC)is of great significance in hydrocarbon exploration and *** controlled experiments with a single variable is challenging for natural shales due to their high variations in components,diagenesis conditions,or pore *** employed the hot-pressing technique to construct 11 well-controlled artificial shale with varying *** artificial shale samples were successive machined into prismatic shape for ultrasonic measurements along different *** revealed bedding perpendicular P-wave velocities are more sensitive to the increasing KC than bedding paralleling velocities due to the preferential alignments of *** elastic stiffnesses except C_(13)are generally decreasing with the increasing KC,the variation of C_(1) and C_(33)on kerogen content are more sensitive than those of C_(44)and C_(66).Apparent dynamic mechanical parameters(v and E)were found to have linear correlation with the true ones from complete anisotropic equations independent of KC,which hold value towards the interpretation of well logs consistently across formations,Anisotropic mechanical parameters(ΔE and brittlenessΔB)tend to decrease with the reducing KC,withΔB showing great sensitivity to KC *** the range of low KC(erve as a significant characterization for highly organic-rich(>10%)shale,compilation of data from natural organic rich-shales globally verified the similar systematic relationships that can be empirically used to predict the fraction of KC in shales.

关键词： Elastic properties Organic shale Anisotropy Kerogen content Physical modeling

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大麻素受体2与几种消化系统疾病

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世界华人消化杂志 2016年第3期24卷 400-407页

作者：张建戴二黑姜慧卿河北省人民医院消化科河北省石家庄市050051 石家庄市第五医院河北省石家庄市050021 河北医科大学第二医院消化科河北省消化病重点实验室河北省消化病研究所河北省石家庄市050000

内源性大麻素系统包括内源性大麻素物质、特异性大麻素受体1(cannabinoid receptor 1,CB1)和CB2.内源性大麻素系统在胃肠道疾病、肝脏疾病、胰腺疾病和肿瘤的发生发展过程中扮演了众多的生理效应和病理生理角色.本文就内源性大麻素系统... 详细信息

内源性大麻素系统包括内源性大麻素物质、特异性大麻素受体1(cannabinoid receptor 1,CB1)和CB2.内源性大麻素系统在胃肠道疾病、肝脏疾病、胰腺疾病和肿瘤的发生发展过程中扮演了众多的生理效应和病理生理角色.本文就内源性大麻素系统、CB2与肠易激综合征、炎症性肠病、胰腺炎、肝脏疾病及消化系肿瘤的关系等作一综述.

关键词：大麻素受体2 肠易激综合征炎症性肠病胰腺炎肝脏疾病消化系肿瘤

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Genotyping of strains of Yersinia pestis from Marmota baibacina-Spermophilus undulates Plague Focus of Tianshan Mountains in China

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Journal of Microbiology and Immunology 2005年第4期3卷 266-269页

作者： LI XIA JIN er hei dai DONG SHENG ZHOU BAI ZHONG CWI RI XIA dai SHAN LONG YUE MEI YING QI HAI HONG ZHAO CUN XIANG LI XIAO YAN YANG XIAO TAO YU XIANG dai MIN LI RUI FU YANG Qinghai Institute for Endemic Diseases Control and Research Xining P. R. China Instttue of Microbiology and Epidemiology Academy of Military Medical Scicences Belting P. R China 3Xinjiang Provincial Center for Dieases Control and Prevention Wulumuqi P. R. China

The aim of this study is to carry out genotyping of 61 Y. pestts strmns tsolated trom lwarmota baibacina-Spermophilus undulates Plague Focus of Tianshan Mountains in China. Primer pairs targeting the 22 DFRs were designed for detecting the genotypes of 61 strains. As a result, 61 strains of Y. pestis were divided into four genotypes 1, 2, 3, and 4. Genotypes 1, 2 and 3 were found from west part in Northern Tianshan Mountains Plague Focus, but type 1 was only from Nileke county. The type of strains from Aheqi was different from those of Atushi counties in Southern Tianshan Mountains and similar to strains in Northern Tianshan Mountains Plague Focus. The type 4 distributed over Atushi county, which was identical with that of strains from Marmota caudae Plague Focus of the Pamiri Plateau. It is concluded that geonotyping is identical with ecotyping made by Shuli Ji. Tianshan Mountains Plague Focus and Marmota caudae Plague Focus of the Pamiri Plateau have a cross spreading profile.

关键词： Yersinia pestis PCR Comparative genomics DFR Plague Focus Natural

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Detection of serum immunoglobulin M and immunoglobulin G antibodies in 2019 novel coronavirus infected patients from different stages

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Chinese Medical Journal 2020年第12期133卷 1479-1480页

作者： Hui-Xia Gao Ya-Nan Li Zun-Gui Xu Yu-Ling Wang Hai-Bin Wang Jin-Feng Cao De-Qin Yuan Li Li Yi Xu Zhi Zhang Ying Huang Jian-Hua Lu Yu-Zhen Liu er-hei dai Department of Laboratory Medicine Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuangHebei 050021China Department of Laboratory Medicine Hospital of XiongxianXiong’an New AreaHebei 071800China Department of Tuberculosis Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuangHebei 050021China Intensive Care Unit Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuangHebei 050021China

To the Editor:The epidemic caused by 2019 novel coronavirus(2019-nCoV)has drawn public attention.[1]Huge infected population and enormous economic loss make it the urgent public health event to deal *** test targeting virus RNA was established at the early stage of outbreak and then used for 2019-nCoV infection diagnosis.[2]However,high risk of laboratory infection,high-qualified personnel,and strict operation condition hampered its application into primary hospitals and community clinics.[3]In this study,the serum immunoglobulin(Ig)M and IgG antibodies were detected in 2019-nCoV confirmed cases of different ***,three different immunological assays,chemiluminescent immunoassay(CLIA),gold immunochromatographic assay(GICA),and enzyme-linked immunosorbent assay(ELISA)were used for IgM and IgG detection.

关键词： infected antibodies immunoglobulin

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Research on Alliance Decision of Dual-Channel Remanufacturing Supply Chain Considering Bidirectional Free-Riding and Cost-Sharing

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Computer Modeling in Engineering & Sciences 2024年第9期140卷 2913-2956页

作者： Lina Dong Yeming dai School of Business Qingdao UniversityQingdaoChina

This study delves into the formation dynamics of alliances within a closed-loop supply chain(CLSC)that encom-passes a manufacturer,a retailer,and an e-commerce *** leverages Stackelberg game for this exploration,contrasting the equilibrium outcomes of a non-alliance model with those of three differentiated alliance *** non-alliance model acts as a crucial benchmark,enabling the evaluation of the motivations for various supply chain entities to engage in alliance *** analysis is centered on identifying the most effective alliance strategies and establishing a coordination within these *** thoroughly investigate the consequences of diverse alliance behaviors,bidirectional free-riding and cost-sharing,and the resultant effects on the optimal decision-making among supply chain *** findings underscore several pivotal insights:(1)The behavior of alliances within the supply chain exerts variable impacts on the optimal pricing and demand of its *** comparison to the non-alliance(D)model,the manufacturer-retailer(MR)and manufacturer-e-commerce platform(ME)alliances significantly lower both offline and online resale prices for new and remanufactured *** adjustment leads to an enhanced demand for products via the MR alliance’s offline outlets and the ME alliance’s online platforms,thereby augmenting the profits for those within the ***,retailer-e-commerce platform(er)alliance tends to increase the optimal retail price and demand across both online and offline *** specific conditions,alliance behavior can also increase the profits of non-alliance members,and the profits derived through alliance channels also exceed those from non-alliance channels.(2)The prevalence of bidirectional free-riding behavior largely remains constant across different alliance *** these models,bidirectional free-riding typically elevates the equilibrium prices in offline channel while negatively affe

关键词： Dual-channel remanufacturing supply chain alliance remanufactured products bidirectional free-riding cost-sharing

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Effect of er_(30)Cu_(70) on microstructure and properties of sintered Nd-Fe-B by grain boundary reconstruction

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Journal of Rare Earths 2024年第2期42卷 345-353,I0004页

作者： Kai Wang Yongtao dai Jianqiu Deng Jiang Wang Huaiying Zhou Qingrong Yao Weichao Huang Guangxi Key Laboratory of Information Materials Schoolof Materials Science and EngineeringGuilin University of Electronic TechnologyGuilin54004China China Rare Metals and Rare Earth(Guangxi)Jinyuan New Materials Co Ltd.Hezhou542603China

Usually it is generally believed that the er element forms the er_(2)Fe_(14)B phase,which will seriously deteriorate the magnetic ***,here we report the balance of corrosion resistance and coercivity in Nd-Fe-B sintered magnets through using simple er_(30)Cu_(70) additive whose price is much lower than Dy and *** reasonably controlling er_(30)Cu_(70) addition,the corrosion resistance is improved at the minimum limit of reducing the magnetic *** studying the influence mechanism of er element,it is found that the main effect of er elements is to replace the Nd elements at the edge of the main phase grains to form a(er,Nd)_(2)Fe_(14)B shell with low H_(A),resulting in the reduction of magnetic *** improvement of corrosion resistance mainly comes from the more stable Cu element introduced at the grain *** the same time,the target magnets also show different advantages under different heat treatment *** findings may spur progress towards developing the lowcost permanent magnets that rival the commercial Nd-Fe-B counterpart.

关键词： er_(30)Cu_(70)alloy Grain boundary reconstruction Magnetic properties Corrosion resistance Heat treatment Rare earths

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