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Trilobatin Acts as a Marker Metabolite Involved in Flavonoid Accumulation Regulated by CsWRKY28-MYC2 with Trypsin Activation in Cucumber (Cucumis sativus)

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 2837-2856页

作者： enyan chen Jingyu Jia Jiaju Sun Jie Wang Xinxin chen Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Chinese Academy of Agricultural Sciences Cotton Research Institute Anyang455000China The National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang Tarim UniversityAlar843300China National Demonstration Center for Experimental Food Processing and Safety Education Luoyang471000China

During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseﬂavonoid compound levels and reduce oxidative *** investigate the mechanism of trypsin-inducedﬂavonoid bio-synthesis in ***,we conducted a combined analysis of transcriptomics and widely targeted *** hundred and seventy-ﬁve signiﬁcantly different metabolites were obtained from metabolomics *** kyoto encyclopedia of genes and genomes(KEGG)functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis *** combining the results of the weighted gene co-expression network analysis(WGCNA)with the 130 upregulated phenylpropanoid metabolites,22 signiﬁcantly upregulated phenylpropanoid metabolites were identiﬁ*** was identiﬁed as the most prominent meta-bolite through cluster analysis and variable importance in projection(VIP)*** performance liquid chro-matography(HPLC)experiments conﬁrmed that trilobatin was the key metabolite induced by *** transcriptomic results showed that 1068 genes in the brown module of WGCNA were highly positively correlated withﬂavonoid *** gene set enrichment analysis(GSEA)identiﬁed leading edges in 4 key KEGG ***,combined with WGCNA and GSEA analysis results,35 core genes were *** co-expression network of transcriptomics and metabolomics suggested that CsWRKY28 and CsMYC2 regulated the biosynthesis of *** quantitative real-time polymerase chain reaction(RT-qPCR)and dual luciferase experiments con-ﬁrmed the activation effect of CsWRKY28 on CsMYC2 and downstream target *** study revealed the key transcription factors involved in the trypsin-controlled biosynthesis of trilobatin in *** and provided a new theoretical basis for elucidating the molecular mechanism of trypsin preservation.

关键词： CsWRKY28 transcriptomics trilobatin trypsin widely targeted metabolomics

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4-Hydroxy-2-Oxoglutaric Acid,A KeyMetabolite Involved in Trypsin-Regulation of Arginine Metabolism in Hylocereus undatus during Storage

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Phyton-International Journal of Experimental Botany 2024年第5期93卷 885-900页

作者： Bairu Li Jingyu Jia Hemin Wang Jiaju Sun enyan chen Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Henan Engineering Research Center of Food Microbiology Luoyang471023China National Demonstration Center for Experimental Food Processing and Safety Education Luoyang471000China

Trypsin,a novel superoxide scavenger,significantly enhances the storage quality of Hylocereus undatus(***).To elucidate the preservation mechanism of trypsin on ***,a widely targeted metabolomic analysis,and transcriptomics analysis were ***,a total of 453 metabolites were identified,with organic acids and their derivatives constituting the largest proportion(25%).Amino acids and their metabolites,prominent among organic acids,were further *** them,73 metabolites were associated with amino acids,and 37 exhibited significant *** most enriched Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway was arginine biosynthesis(map00220),with polyamine metabolites showing the most pronounced differences,particularly spermine(FC=1.7594).Compared with the control group,4-hydroxy-2-oxoglutaric acid was significantly upregulated(FC=2.117)in the process of spermine ***,the results of Gene Ontology(GO)and KEGG enrichment analysis of the *** transcriptome profile revealed that trypsin treatment led to 187 differentially expressed genes associated with *** GO and KEGG analyses exhibited significant enrichment in the spermine biosynthetic process(GO:0006597)(map:00220)within the arginine biosynthesis ***,most enzymes and metabolites within the spermine biosynthesis pathway in *** were *** results of the PPI network highlighted that ADC,SPDS,and SAMDC,among others,were pivotal proteins involved in trypsin-regulated arginine metabolism and spermine *** study revealed that trypsin could significantly delay postharvest senescence of *** at room *** effect might be attributed to trypsin triggering the synthesis of 4-hydroxy-2-oxoglutaric acid in the fruit peel,thereby promoting the biosynthesis of spermine and other polyamines.

关键词： Trypsin arginine transcriptomics widely targeted metabolomics

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EuSHT Acts as a Hub Gene Involved in the Biosynthesis of 6-Hydroxyluteolin and Quercetin Induced by Salt Stress in Eucommia ulmoides

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Phyton-International Journal of Experimental Botany 2024年第8期93卷 2095-2113页

作者： Fuxin Li enyan chen Xinxin chen Jingyu Jia Hemin Wang Jie Zhang Jianrui Sun Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Henan Engineering Research Center of Food Microbiology Luoyang471023China National Demonstration Center for Experimental Food Processing and Safety Education Luoyang471000China

Salt stress inhibits plant growth and affects the biosynthesis of its secondary *** are natural compounds that possess many important biological activities,playing a significant role in the medicinal activity of Eucommia ulmoides(***).To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in ***,a comprehensive analysis of metabolomics and transcriptomics was *** results indicated that salt stress led to the wilting and darkening of *** leaves,accompanied by a decrease in chlorophyll levels,and significantly induced malondialdehyde(MDA)and relative electrical *** salt stress,most metabolites in the flavonoid biosynthesis pathway of *** were upregulated,indicating that flavonoid biosynthesis is likely the main induced pathway under salt *** them,secondary metabolites such as 6-Hydroxyluteolin and Quercetin are likely key metabolites induced by salt *** correlation analysis of transcriptomics and metabolomics revealed that EuSHT is a hub gene induced by salt stress,promoting the production of flavonoids such as *** co-expression network showed a strong positive correlation between EuSHT and the biosynthesis of 6-Hydroxyluteolin and Quercetin,while it exhibited a negative correlation with Catechin *** branches leading to Luteolin and Dihydroquercetin are likely the main pathways for flavonoid compound biosynthesis in the plant stress response during salt *** results of this study provided a preliminary mechanism of secondary metabolites such as flavonoids in the medicinal plant *** induced by salt stress and provided new theoretical support for discussing the mechanism of plant stress *** also provided useful information for subsequent exploration of resistance genes in ***.

关键词： Eucommia ulmoides flavonoid salt stress transcriptomics widely targeted metabolomics

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低剂量螺旋CT肺结节扫描与常规剂量CT的对照研究

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当代医学 2019年第19期25卷 157-159页

作者：曾伟胜陈恩炎林杰普宁市人民医院CT科

目的对低剂量螺旋CT肺结节扫描与常规剂量CT肺结节扫描进行对照研究,并对低剂量螺旋CT肺结节扫描的诊断价值进行评价。方法选取2018年1月至2018年12月经本院诊疗的62例肺结节患者为研究对象,所有患者均给予低剂量螺旋CT肺结节扫描和常... 详细信息

目的对低剂量螺旋CT肺结节扫描与常规剂量CT肺结节扫描进行对照研究,并对低剂量螺旋CT肺结节扫描的诊断价值进行评价。方法选取2018年1月至2018年12月经本院诊疗的62例肺结节患者为研究对象,所有患者均给予低剂量螺旋CT肺结节扫描和常规剂量螺旋CT肺结节扫描。对两种扫描检查方式所形成的图像质量、肺结节形态学以及肺结节检出的情况进行对比分析。结果低剂量螺旋CT扫描所获取的图像质量中,A级54例,占比87.10%,B级6例,占比9.68%,C级2例,占比3.22%;常规剂量螺旋CT扫描所获取的图像质量中,A级53例,占比85.49%,B级7例,占比11.29%,C级2例,占比3.22%。两种扫描方式在图像质量方面差异无统计学意义;在扫描到的结节数量方面,低剂量螺旋CT共扫描98个,伴毛刺征37个,占比37.76%,分叶征28例,占比28.57%,胸膜凹陷征23例,占比23.47%,血管聚集征10例,占比10.20%;常规剂量螺旋CT共扫描96个,伴毛刺征35个,占比36.46%,分叶征29例,占比30.21%,胸膜凹陷征24例,占比25%,血管聚集征8例,占比8.33%,两种扫描方式在肺结节数量以及具体形态方面差异无统计学意义;在图像噪声方面,低剂量CT扫描高于常规剂量CT扫描,但在辐射剂量方面,低剂量CT扫描明显低于常规剂量CT扫描,两种扫描方式在图像噪声和辐射剂量方面差异有统计学意义(P<0.05)。结论低剂量螺旋CT肺结节扫描与常规剂量扫描相比,在肺结节检出方面以及图像质量方面未形成明显差异,均能够有效的对肺结节进行初筛和诊断,但低剂量能够使患者所受辐射伤害减少,值得推广应用。

关键词：低剂量螺旋CT 常规剂量肺结节

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增强CT直方图分析与肺癌组织分化程度的相关性研究

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医学影像学杂志 2017年第9期27卷 1698-1700页

作者：彭晓容马春浓陈恩炎广东省普宁市人民医院放射科广东普宁515300 南方医科大学第三附属医院放射科广东广州510630

目的肺癌组织的分化程度对评估肿瘤生物学行为、预后及治疗方式具有重要意义。本研究拟探讨增强CT直方图分析与肺癌组织分化程度的相关性。方法收集我院经手术病理证实的肺癌患者105例,均行CT增强扫描。采用Image J软件手动在肿瘤增强C... 详细信息

目的肺癌组织的分化程度对评估肿瘤生物学行为、预后及治疗方式具有重要意义。本研究拟探讨增强CT直方图分析与肺癌组织分化程度的相关性。方法收集我院经手术病理证实的肺癌患者105例,均行CT增强扫描。采用Image J软件手动在肿瘤增强CT最大层面画取感兴趣区(region of Interest,ROI),测量直方图各参数(平均值、SD、峰度、偏度)。采用单因素方差分析比较不同分化程度间的直方图分析各参数值得差异,用Spearman相关系数分析直方图各参数与不同分化程度之间的相关性。结果高、中、低分化组肺癌的偏度值、峰度值有统计学差异(P<0.001)。高分化组的偏度、峰度绝对值均较中、低分化组肺癌低(P<0.001)。三组肺癌的平均数及SD无统计学差异。偏度值及峰度值与病变不同分化程度存在相关性(偏度值r=-0.267,P=0.006;峰度值r=0、381,P<0.001)。结论增强CT的直方图分析偏度值及峰度值可术前判断肺癌的分化程度。

关键词：肺癌直方图分析分化程度体层摄影术,X线计算机

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多排螺旋CT与MR对胰腺癌的诊断价值探讨

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临床医学工程 2017年第3期24卷 325-326页

作者：彭晓容马春浓陈恩炎黄海涛广东省普宁市人民医院放射科广东普宁515300 南方医科大学附属第三医院广东广州510631

目的探讨多排螺旋CT与MR在胰腺癌诊断中的应用价值。方法选取我院2014年1月至2016年8月收治的70例胰腺癌患者,随机分为两组,每组35例。CT组采用多排螺旋CT扫描,MR组则采用MR扫描。两组均与术后病理学诊断结果进行比较,评价两组扫描检查... 详细信息

目的探讨多排螺旋CT与MR在胰腺癌诊断中的应用价值。方法选取我院2014年1月至2016年8月收治的70例胰腺癌患者,随机分为两组,每组35例。CT组采用多排螺旋CT扫描,MR组则采用MR扫描。两组均与术后病理学诊断结果进行比较,评价两组扫描检查的准确性、敏感度、特异性以及不同时期的显示值。结果 (1)CT组的准确性、敏感度和特异性分别为88.57%、82.86%和74.29%,与MR组的91.43%、80.00%和71.43%比较,差异无统计学意义(P>0.05)。(2)MR组胰腺动脉期、静脉期和延迟期的显示值均显著高于CT组,差异均有统计学意义(P<0.05)。结论多排螺旋CT与MR在胰腺癌诊断中均具有良好的准确性、敏感度和特异性以及较高的应用价值,但在不同时期的显示方面,MR优于多排螺旋CT。

关键词：多排螺旋CT MR 胰腺癌临床诊断应用价值

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Effect of cobalt oxide on performance of Pd catalysts for lean-burn natural gas vehicles in the presence and absence of water vapor

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Journal of Natural Gas Chemistry 2010年第2期19卷 134-138页

作者： enyan Long Xiaoyu Zhang Yile Li Zhimin Liu Yun Wang Maochu Gong Yaoqiang chen Key Laboratory of Green Chemistry ＆ Technology Ministry of Education College of Chemistry Sichuan University Chengdu 610064 Sichuan China

Pd-based catalysts modified by cobalt were prepared by co-impregnation and sequential impregnation methods,and characterized by X-ray powder diffraction （XRD）,N2 adsorption/desorption （Brunauer-Emmet-Teller method）,CO-chemisorption and X-ray photoelectron spectroscopy （XPS）.The activity of Pd catalysts was tested in the simulated exhaust gas from lean-burn natural gas *** effect of Co on the performance of water poisoning resistance for Pd catalysts was estimated in the simulated exhaust gas with and without the presence of water *** was found that the effect of Co significantly depended on the preparation ***/La-Al2O3 catalyst prepared by co-impregnation exhibited better water-resistant *** results of XPS indicated that both CoAl2O4 and Co3O4 were present in the Pd catalysts modified by *** the catalyst prepared by sequential impregnation method,the ratio of CoAl2O4/Co3O4 was higher than that of the catalyst prepared by co-impregnation *** could be concluded that Co3O4 played an important role in improving water-resistant performance.

关键词： Co Pd-only catalyst lean-burn natural gas vehicles methane oxidation water vapor

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CeO_2-ZrO_2-La_2O_3-Al_2O_3 composite oxide and its supported palladium catalyst for the treatment of exhaust of natural gas engined vehicles

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Journal of Natural Gas Chemistry 2009年第2期18卷 139-144页

作者： Xiaoyu Zhang enyan,Long Yile Li Jiaxiu Guo Lijuan Zhang Maochu Gong Minghua Wang Yaoqiang chen Key Laboratory of Green Chemistry ＆ Technology of the Ministry of Education College of Chemistry Sichuan University Chengdu 610064 Sichuan China College of Architecture and Environment Sichuan University Chengdu 610064 Sichuan China State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 Liaoning China Sichuan Tianyi Science ＆ Technology Co. Ltd. Chengdu 610225 Sichuan China

Composite supports CeO2-ZrO2-Al2O3（CZA） and CeO2-ZrO2-Al2O3-La2O3（CZALa） were prepared by co-precipitation method. Palladium catalysts were prepared by impregnation and their purification ability for CH4, CO and NOx in the mixture gas simulated the exhaust from natural gas vehicles （NGVs） operated under stoichiometric condition was investigated. The effect of La2O3 on the physicochemical properties of supports and catalysts was characterized by various techniques. The characterizations with X-ray diffraction （XRD） and Raman spectroscopy revealed that the doping of La2O3 restrained effectively the sintering of crystallite particles, maintained the crystallite particles in nanoscale and stabilized the crystal phase after calcination at 1000 ℃. The results of N2-adsorption, H2-temperatnre-programmed reduction （H2-TPR） and oxygen storage capacity （OSC） measurements indicated that La2O3 improved the textural properties, reducibility and OSC of composite supports. Activity testing results showed that the catalysts exhibit excellent activities for the simultaneous removal of methane, CO and NOx in the simulated exhaust gas. The catalysts supported on CZALa showed remarkable thermal stability and catalytic activity for the three pollutants, especially for NOx. The prepared palladium catalysts have high ability to remove NOx, CH4 and CO, and they can be used as excellent catalysts for the purification of exhaust from NGVs operated under stoichiometric condition. The catalysts reported in this work also have significant potential in industrial application because of their high performance and low cost.

关键词： Pd catalysts natural gas vehicles exhaust methane oxidation NOx conversion CeO2-ZrO2-Al2O3 La2O3

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Influence of CeO_2 and La_2O_3 on properties of palladium catalysts used for emission control of natural gas vehicles

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Journal of Natural Gas Chemistry 2009年第4期18卷 415-420页

作者： Yile Li Xiaoyu Zhang enyan Long Hongmei Li Dongdong Wu Li Cai Maochu Gong Yaoqiang chen Key Laboratory of Green Chemistry ＆ Technology of the Ministry of Education College of Chemistry Sichuan University Chengdu 610064 Sichuan China

Pd/YZ-Al2O3 （Y and Zr modified Al2O3, and hereafter, labelled as A1） catalysts with 4 wt% additive CeO2 and/or La2O3 were prepared and characterized by X-ray photoelectron spectroscopy （XPS）, NO-temperature programmed desorption （NO-TPD）, Nz-adsorption/desorption （Branauer-Emmet-Teller BET method）, X-ray diffraction （XRD） and CO-chemisorption. Catalytic activities for CH4, CO and NO conversion were tested in a gas mixture simulated the emissions from natural gas vehicles （NGVs） operated under stoichiometric conditions. The results indicated that all catalysts exhibited excellent catalytic performances for CH4 and CO oxidation and the promoting effect of CeO2 or La2O3 was significant for NO conversion. XPS results showed that the electron density around Pd was increased by CeO2 and/or La2O3, the binding energy of Pd 3d decreased as the order： Pd/Al 〉 Pd/Ce/Al 〉 Pd/La/Al 〉 Pd/CeLa/Al. The electron-rich Pd showed Rh-like catalytic properties which exhibited good activity for the reduction of NO. NO-TPD results showed that the addition of CeO2 and/or La2O3 increased NO adsorption on surface, and promoted the conversion of NO.

关键词： NGVs under stoichiometric condition palladium catalyst XPS NO-TPD NO conversion methane conversion

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Facilitating sulfur species capture and bi-directional redox in Li-S batteries with single-atomic Co-O_(2)N_(2) coordination structure

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Journal of Energy Chemistry 2024年第12期99卷 604-614页

作者： Yunqiang Zhang Huaming Yuan enyan Guo Shunwei chen Manman Ren Jingyun Ma Jiaxi Cui Mei Li Laiying Jing Li Li School of Materials Science and Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353ShandongChina Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054SichuanChina School of Metallurgy Northeastern UniversityShenyang 110819LiaoningChina

Lithium-sulfur(Li-S)batteries suffer from the shuttle effect of soluble lithium polysulfides(LiPSs)and slow redox kinetics,significantly limiting their practical *** single-atom catalysts(SACs)offer a promising strategy to address these challenges,designing materials with optimal adsorption force and high catalytic activity remains a grand ***,we present a cobalt(Co)-based SAC with unique Co-O_(2)N_(2) coordination structures for Li-S *** experimental and theoretical studies demonstrate that O,N-coordinated Co single atoms anchored on a porous carbon framework(Co/NOC)effectively capture LiPSs and dramatically catalyze bidirectional polysulfide *** expanded carbon layer spacing facilitates lithium ions diffusion and maximizes the exposure of active *** a result,Li-S batteries incorporating Co/NOC as separators exhibit outstanding rate performance(906.6m Ah g^(-1)at 3 C)and exceptional cycling stability,even at-10℃.Furthermore,with a high sulfur loading of 12.0 mg cm^(-2),the areal specific capacity reaches up to 12.36 mAh cm^(-2).This work provides some useful insights for the design of high-performance SACs for Li-S batteries.

关键词： Asymmetrically coordinated Co-O_(2)N_(2) Zeolitic imidazolate framework-67 Melamine resin Carbon nanoflakes Shuttle effect

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