检索结果-南通市图书馆

Metadherin promotes stem cell phenotypes and correlated with immune infiltration in hepatocellular carcinoma

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第8期30卷 901-918页

作者： Yi-Ying Wang mei-mei Shen Jian Gao Department of Gastroenterology The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China Department of Gastroenterology and Hepatology The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China

BACKGROUND Metadherin(MTDH)is a key oncogene in most cancer types,including hepato-cellular carcinoma(HCC).Notably,MTDH does not affect the stemness pheno-type or immune infiltration of HCC.AIM To explore the role of MTDH on stemness and immune infiltration in HCC.METHODS MTDH expression in HCC tissues was detected using TCGA and GEO databases.Immunohistochemistry was used to analyze the tissue samples.MTDH was stably knocked down or overexpressed by lentiviral transfection in the two HCC cell lines.The invasion and migration abilities of HCC cells were evaluated using Matrigel invasion and wound healing assays.Next,we obtained liver cancer stem cells from the spheroids by culturing them in a serum-free medium.Gene expression was determined by western blotting and quantitative reverse transcri-ption PCR.Flow cytometry,immunofluorescence,and tumor sphere formation assays were used to characterize stem-like cells.The effects of MTDH inhibition on tumor growth were evaluated in vivo.The correlation of MTDH with immune cells,immunomodulators,and chemokines was analyzed using ssGSEA and TISIDB databases.RESULTS HCC tissues expressed higher levels of MTDH than normal liver tissues.High MTDH expression was associated with a poor prognosis.HCC cells overex-pressing MTDH exhibited stronger invasion and migration abilities,exhibited a stem cell-like phenotype,and formed spheres;however,MTDH inhibition attenuated these effects.MTDH inhibition suppressed HCC progression and CD133 expression in vivo.MTDH was positively correlated with immature dendritic,T helper 2 cells,central memory CD8^(+)T,memory B,activated dendritic,natural killer(NK)T,NK,activated CD4^(+)T,and central memory CD4^(+)T cells.MTDH was negatively correlated with activated CD8^(+)T cells,eosinophils,activated B cells,monocytes,macrophages,and mast cells.A positive correlation was observed between the MTDH level and CXCL2 expression,whereas a negative correlation was observed between the MTDH level and CX3CL1 and CXCL12 expression.CONCLUSION High levels of M

关键词： Metadherin Hepatocellular carcinoma Cancer stem cells Immune infiltration

Oligomerization of drug transporters:Forms,functions,and mechanisms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第5期14卷 1924-1938页

作者： Chunxu Ni mei Hong College of Life Sciences South China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642China

Drug transporters are essential players in the transmembrane transport of a wide variety of clinical drugs.The broad substrate spectra and versatile distribution pattern of these membrane proteins infer their pharmacological and clinical significance.With our accumulating knowledge on the three-dimensional structure of drug transporters,their oligomerization status has become a topic of intense study due to the possible functional roles carried out by such kind of post-translational modification(PTM).In-depth studies of oligomeric complexes formed among drug transporters as well as their interactions with other regulatory proteins can help us better understand the regulatory mechanisms of these membrane proteins,provide clues for the development of novel drugs,and improve the therapeutic efficacy.In this review,we describe different oligomerization forms as well as their structural basis of major drug transporters in the ATP-binding cassette and solute carrier superfamilies,summarize our current knowledge on the influence of oligomerization for protein expression level and transport function of these membrane proteins,and discuss the regulatory mechanisms of oligomerization.Finally,we highlight the challenges associated with the current oligomerization studies and propose some thoughts on the pharmaceutical application of this important drug transporter PTM.

关键词： Drug development Drug transporters Oligomerization Protein expression Protein–protein interaction Regulatory mechanism Structural basis Transport function

Effect of short neuropeptide F signaling on larval feeding in Mythimna separata

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2024年第2期31卷 417-434页

作者： mei-mei Li Qi Yang Li-Hui Chen Yan-Ying Li Jun-Xiang Wu Xiang-Li Xu State Key Laboratory of Crop Stress Biology in Arid Areas College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi ProvinceChinaand School of Agricultural Sciences Jiangxi Agricultural UniversityNanchangChina

Mythimna separata is a notorious phytophagous pest which poses serious threats to cereal crops owing to the gluttony of the larvae.Because short neuropeptide F(sNPF)and its receptor sNPFR are involved in a diversity of physiological functions,especially in functions related to feeding in insects,it is a molecular target for pest control.Herein,an sNPF and 2 sNPFRs were identified and cloned from M.separata.Bioinformatics analysis revealed that the sNPF and its receptors had a highly conserved RLRFamide C-terminus and 7 transmembrane domains,respectively.The sNPF and its receptor genes were distributed across larval periods and tissues,but 2 receptors had distinct expression patterns.The starvation-induced assay elucidated that sNPF and sNPFR expression levels were downregulated under food deprivation and recovered with subsequent re-feeding.RNA interference knockdown of sNPF,sNPFR1,and sNPFR2 by injection of double-stranded RNA into larvae not only suppressed food consumption and increased body size and weight,but also led to decrease of glycogen and total lipid contents,and increase of trehalose compared with double-stranded green fluorescent protein injection.Furthermore,molecular docking was performed on the interaction mode between sNPFR protein and its ligand sNPF based on the 3-dimensional models constructed by AlphaFold;the results indicated that both receptors were presumably activated by the mature peptide sNPF-2.These results revealed that sNPF signaling played a considerably vital role in the feeding regulation of M.separata and represents a potential control target for this pest.

关键词： feeding regulation Mythimna separata pest control target RNA interference short neuropeptide F sNPF receptors

Characterizations of semihoops based on derivations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applied Mathematics(A Journal of Chinese Universities) 2024年第2期39卷 291-310页

作者： WANG mei ZHANG Xiao-hong School of Electrical and Control Engineering Shaanxi University of Science and TechnologyXi'an 710021China School of Arts and Sciences Shaanxi University of Science and TechnologyXi'an 710021China School of Mathematics and Statistics Weinan Normal UniversityWeinan 714099China

In this paper,we discuss the related properties of some particular derivations in semihoops and give some characterizations of them.Then,we prove that every Heyting algebra is isomorphic to the algebra of all multiplicative derivations and show that every Boolean algebra is isomorphic to the algebra of all implicative derivations.Finally,we show that the sets of multiplicative and implicative derivations on bounded regular idempotent semihoops are in oneto-one correspondence.

关键词： (prelinear)semihoop derivation Heyting algebra Galois connection

Evaluation of the effects of health education interventions for hypertensive patients based on the health belief model

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第15期12卷 2578-2585页

作者： Hong-mei Wang Ying Chen Yan-Hua Shen Xiao-mei Wang Department of Emergency Lishui Second People's HospitalLishui 323000Zhejiang ProvinceChina Department of Pancreatic and Biliary Surgery The First Affiliated Hospital of China Medical UniversityShenyang 110002Liaoning ProvinceChina

BACKGROUND Hypertension is a major risk factor for cardiovascular disease and stroke,and its prevalence is increasing worldwide.Health education interventions based on the health belief model(HBM)can improve the knowledge,attitudes,and behaviors of patients with hypertension and help them control their blood pressure.AIM To evaluate the effects of health education interventions based on the HBM in patients with hypertension in China.METHODS Between 2021 and 2023,140 patients with hypertension were randomly assigned to either the intervention or control group.The intervention group received health education based on the HBM,including lectures,brochures,videos,and counseling sessions,whereas the control group received routine care.Outcomes were measured at baseline,three months,and six months after the intervention and included blood pressure,medication adherence,self-efficacy,and perceived benefits,barriers,susceptibility,and severity.RESULTS The intervention group had significantly lower systolic blood pressure[mean difference(MD):-8.2 mmHg,P<0.001]and diastolic blood pressure(MD:-5.1 mmHg,P=0.002)compared to the control group at six months.The intervention group also had higher medication adherence(MD:1.8,P<0.001),self-efficacy(MD:12.4,P<0.001),perceived benefits(MD:3.2,P<0.001),lower perceived barriers(MD:-2.6,P=0.001),higher perceived susceptibility(MD:2.8,P=0.002),and higher perceived severity(MD:3.1,P<0.001)than the control group at six months.CONCLUSION Health education interventions based on the HBM effectively improve blood pressure control and health beliefs in patients with hypertension and should be implemented in clinical practice and community settings.

关键词： Hypertension Health education Health belief model Blood pressure control Randomized controlled trial

Process in laser drilling of deep microholes without taper on metal materials

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2024年第1期67卷 37-59页

作者： FAN RunZe mei XueSong CUI JianLei State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710054China

Deep microhole machining is currently a prominent research area within the aerospace field,encompassing blade film cooling and fuel injection control technologies.However,taper defects in metal materials may lead to performance degradation or even structural damage over a component’s lifetime.Trepanning and helical drilling,facilitated by ultrashort pulse lasers,have proven more suitable for achieving high-precision,deep holes in metal materials.Nonetheless,excessive repetition rates can also result in severe thermal damage.Various methods are commonly employed for controlling taper,including parameter optimization,assistance,and secondary modification.Tilted laser beam drilling is widely utilized and has been integrated into relevant machining systems for commercial applications.Typical deep microholes include film cooling holes and injection microholes.Laser drilling is a potential machining method for new materials in the aerospace field.Although laser drilling processing has been studied,numerous related scientific challenges and technical difficulties must be addressed before practical implementation.

关键词： aerospace laser drilling deep microhole taper control

Compensation temperatures and hysteresis behaviors of a graphene-like trilayer

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2024年第5期76卷 166-179页

作者： Ying An Ji Liang Wei Wang mei-mei Du Feng-ge Zhang School of Science Shenyang University of TechnologyShenyang 110870China School of Electrical Engineering Shenyang University of TechnologyShenyang 110870China

This work focuses on the ground-state phase diagram,the compensation temperatures and the critical behaviors of a ferrimagnetic graphene-like trilayer induced by crystal fields and exchange couplings.The simulation results show that a negative decrease in crystal field or an increase in exchange coupling can increase the critical temperature.More importantly,an M curve with double compensation temperatures can be observed,which is not predicted by the Ne´el theory.This remarkable compensation phenomenon has potential application value in the field of magnetic recording.

关键词： graphene-like trilayer mixed-spin Ising model magnetization double compensation temperatures triple-loop hysteresis monte Carlo

Evaluation of the value of combined detection of tumor markers CA724,carcinoembryonic antigen,CA242,and CA19-9 in gastric cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第5期16卷 1737-1744页

作者： Chong-mei Zhou Shao-Hua Zhao Department of Clinical Laboratory Henan Provincial People's HospitalZhengzhou 450003Henan ProvinceChina

BACKGROUND Gastric cancer is a global health concern that poses a significant threat to human well-being.AIM To detecting serum changes in carcinoembryonic antigen(CEA),carbohydrate antigens(CA)724,CA242,and CA19-9 expression among patients with gastric cancer.METHODS Eighty patients diagnosed with gastric cancer between January 2020 and January 2023 were included in the observation group,while 80 patients with benign gastric diseases were included in the control group.Both groups were tested for tumor markers(CA724,CEA,CA242,and CA19-9].Tumor marker indicators(CA724,CEA,CA242,and CA19-9)were compared between the two groups,assessing positive rates of tumor markers across various stages in the observation group.Additionally,single and combined detection of various tumor markers were examined.RESULTS The sensitivity,specificity,accuracy,positive predictive value,and negative predictive value observed for the combined detection of CA724,CEA,CA242,and CA19-9 were higher than those of CA724,CEA,CA242,and CA19-9 individually.Therefore,the combined detection of CA724,CEA,CA242,and CA19-9 has a high diagnostic accuracy and could reduce the occurrence of missed or misdiagnosed cases,facilitating the early diagnosis and treatment of patients.CONCLUSION CA724,CEA,CA242,and CA19-9 serum levels in gastric cancer patients significantly surpassed those in non-gastric cancer patients(P<0.05).Their combined detection can improve the diagnostic accuracy for gastric cancer,warranting clinical promotion.

关键词： Biomarkers Serum Gastric cancer Diagnostic value

Ultrastable Gd^(3+)doped CsPbCl_(1.5)Br_(1.5) nanocrystals blue glass for regulated and low thresholds amplified spontaneous emission

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Photonics Research 2021年第10期9卷 1916-1923页

作者： QINGYUN HE enrou mei ZE WANG XIAOJUAN LIANG SUQIN CHEN WEIDONG XIANG College of Chemistry and Materials Engineering Wenzhou UniversityWenzhou 325035China

Here,Gd-doped CsPbCl_(1.5)Br_(1.5) nanocrystals(NCs)in borosilicate glass matrix(B_(2)O_(3)−SiO_(2)−ZnO)were prepared by melting quenching and in-situ crystallization.The optical performance of CsPbCl_(1.5)Br_(1.5) NCs glasses under different heat-treatment temperatures and the content of Gd^(3+) were analyzed in detail.After CsPbCl_(1.5)Br_(1.5) NCs glass is doped with Gd^(3+)ions,the photoluminescence intensity increases and the synthesized Gd-doped CsPbCl_(1.5)Br_(1.5) NCs glasses have excellent water stability and thermal cycling performance.In addition,the influence of Gd-doped concentrations and heat-treatment temperatures on the amplified spontaneous emission(ASE)thresholds of CsPbCl_(1.5)Br_(1.5) NCs glasses was studied,and the Gd-doped CsPbCl_(1.5)Br_(1.5) NCs glasses achieve controllable ASE thresholds at room temperature.The ASE threshold can be as low as 0.39 mJ∕cm^(2).This work offers a neoteric reference for the research in the application of metal ion-doped perovskite NCs and a new idea for the realization of controllable and low ASE thresholds on perovskite NCs.

关键词： glasses glass temperature.