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GUN4 Is Required for Posttranslational Control of Plant Tetrapyrrole Biosynthesis

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Molecular Plant 2009年第6期2卷 1198-1210页

作者： enrico Peter Bernhard Grimm Institute of Biology/Plant Physiology Humboldt University Philippstr. 13 Building 12 D 10115 Berlin Germany

In aerobic photosynthetic organisms, GUN4 binds the chlorophyll intermediates protoporphyrin and Mg protoporphyrin, stimulates Mg chelatase activity, and is implicated in plastidic retrograde signaling. GUN4 expression is most abundant in young and greening tissues and parallels the activity of 5-aminolevulinic acid （ALA） ALA and Mg porphyrin biosynthesis during photoperiodic growth. We explored function and mode of action of GUN4 using GUN4- deficient and overexpressing plants. GUN4 overexpression leads to a general activation of the enzymes of chlorophyll biosynthesis. During photoperiodic growth GUN4 deficiency prevents ALA synthesis and chlorophyll accumulation. All these metabolic changes do not correlate with altered gene expression or changes of protein abundance in tetrapyrrole biosynthesis. While ALA feeding fails to compensate GUN4 deficiency during light-dark growth, this approach results in chlorophyll accumulation under continuous dim light. A new model defines the involvement of GUN4 in posttranslational regulation of ALA and Mg porphyrin synthesis, to sustain chlorophyll synthesis, namely under varying environmental conditions.

关键词： Tetrapyrrole biosynthesis chlorophyll Mg porphyrin branch photo-oxidation chloroplasts

Advanced modeling of embedded piezo-electric transducers for the health-monitoring of layered structures

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International Journal of Smart and Nano Materials 2020年第4期11卷 325-342页

作者： enrico Zappino Erasmo Carrera Department of Mechanical and Aerospace Engineering Politecnico di TorinoMul2 Research GroupTorinoItaly

The present paper presents an innovative approach for the numerical modeling of piezo-electric transducers for the health-monitoring of layered *** numerical approach has been developed in the frameworks of the Carrera Unified *** computa-tional tool allows refined numerical models to be derived in a unified and efficient *** use of higher-order models and the cap-ability to connect different kinematic models using the node-depen-dent kinematic approach has led to an efficient modeling technique for global-local *** approach can refine the model only in those regions where it is required,e.g.,the areas where piezo-electric transducers are *** model has been used to study embedded and surface-mounted *** accuracy of the pre-sent model has been verified by comparing the current results with numerical and experimental data from the *** mod-eling solutions have been developed,mixing one-,two-and threedimensional finite *** results show that the use of the present modeling technique allows the computational cost to be reduced with respect to the classical approaches preserving the ccuracy of the results in the critical areas.

关键词： Carrera Unified Formulation Embedded Sensor Health Monitoring Layered Structures Refined models

Membrane Engineering for Green Process Engineering

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Engineering 2017年第3期3卷 290-298页

作者： Francesca Macedonio enrico Drioli Institute on Membrane Technology (ITM-CNR ) University of Calabria Rende 87036 Italy Department of Environmental and Chemical Engineering University of Calabria Rende 87036 Italy Department of Energy Engineering College of Engineering Hanyang University Seoul 133-791 Korea Center of Excellence in Desalination Technology King Abdulaziz University Jeddah 21589 Saudi Arabia

绿色过程工程是实现工业可持续发展的一个重要途径。基于过程强化原则,它涉及新设备和新过程方法,期望能够给化学及其他生产领域和过程领域带来根本进步,比如降低生产成本、减小设备尺寸、降低能耗、减少废物产生及改进远程控制、信息... 详细信息

绿色过程工程是实现工业可持续发展的一个重要途径。基于过程强化原则,它涉及新设备和新过程方法,期望能够给化学及其他生产领域和过程领域带来根本进步,比如降低生产成本、减小设备尺寸、降低能耗、减少废物产生及改进远程控制、信息流控制和过程弹性等。膜技术为过程强化原则做出了很大的贡献,在过去这些年,其潜力获得了广泛的认可。本文综合分析了膜技术在水处理、能源生产和天然材料提取等工业领域的应用和前景,重点强调了创新膜集成单元的协同使用存在的机遇,介绍了集成膜系统在海水淡化与原材料生产耦合工艺中的一个应用案例。本文将说明膜工程在实现"零排放""原材料全利用"和"低能耗"等目标中的作用。

关键词：膜工程能源/水/原材料生产超越反渗透法海水淡化

Reactive pathways toward parasitic release of singlet oxygen in metal-air batteries

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npj Computational Materials 2021年第1期7卷 1135-1142页

作者： Adriano Pierini Sergio Brutti enrico Bodo Department of Chemistry University of Rome“La Sapienza”RomeItaly GISEL—Centro di Riferimento Nazionale per i Sistemi di Accumulo Elettrochimico di Energia FirenzeItaly

The superoxide disproportionation reaction is a key step in the chemistry of aprotic metal oxygen batteries that controls the peroxide formation upon discharge and opens the way for singlet oxygen *** we clarify the energy landscape of the disproportionation of superoxide in aprotic media catalyzed by group 1A *** analysis is based on ab initio multireference computational methods and unveils the competition between the expected reactive path leading to peroxide and an unexpected reaction channel that involves the reduction of the alkaline *** channels lead to the release of triplet and singlet O_(2).The existence of this reduction channel not only facilitates singlet oxygen release but leads to a reactive neutral solvated species that can onset parasitic chemistries due to their well-known reducing ***,we show that the application of moderate overpotentials makes both these channels accessible in aprotic batteries.

关键词： properties release singlet

面向城镇住宅建筑光伏一体化的GIS开放数据协同仿真平台

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Engineering 2023年第7期26卷 198-213,I0008页

作者： Marco Massano enrico Macii Andrea Lanzini Edoardo Patti Lorenzo Bottaccioli Interuniversity Department of Regional and Urban Studies and Planning Politecnico di TorinoTorino 10129Italy Department of Energy“Galileo Ferraris” Politecnico di TorinoTorino 10129Italy Department of Control and Computer Engineering Politecnico di TorinoTorino 10129Italy

The rising awareness of environmental issues and the increase of renewable energy sources(RESs)has led to a shift in energy production toward RES,such as photovoltaic(PV)systems,and toward a distributed generation(DG)model of energy production that requires systems in which energy is generated,stored,and consumed *** this work,we present a methodology that integrates geographic information system(GIS)-based PV potential assessment procedures with models for the estimation of both energy generation and consumption *** particular,we have created an innovative infrastructure that co-simulates PV integration on building rooftops together with an analysis of households’electricity *** model relies on high spatiotemporal resolution and considers both shadowing effects and real-sky conditions for solar radiation *** integrates methodologies to estimate energy demand with a high temporal resolution,accounting for realistic populations with realistic consumption *** a solution enables concrete recommendations to be drawn in order to promote an understanding of urban energy systems and the integration of RES in the context of future smart *** proposed methodology is tested and validated within the municipality of Turin,*** the whole municipality,we estimate both the electricity absorbed from the residential sector(simulating a realistic population)and the electrical energy that could be produced by installing PV systems on buildings’rooftops(considering two different scenarios,with the former using only the rooftops of residential buildings and the latter using all available rooftops).The capabilities of the platform are explored through an in-depth analysis of the obtained *** power and energy profiles are presented,emphasizing the flexibility of the resolution of the spatial and temporal *** energy indicators are presented for the self-consumption of produced energy and the avoidance of CO_(

关键词： Energy informatics Geographic information system Load estimation Open data Photovoltaic Residential Urban planning Co-simulation

Fabrication and Testing of Self Cleaning Dry Adhesives Utilizing Hydrophobicity Gradient

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Journal of Bionic Engineering 2015年第2期12卷 270-275页

作者： enrico Bovcro Jeffrey Krahn Carlo Menon MENRVA Lab School of Engineering Science Simon Fraser University 8888 University Drive Burnaby BC Canada V5A 1S6

In this paper we present a method to create a hydrophobicity gradient on the surface of a Polydimethylsiloxane （PDMS） dry adhesive. The method consists of the partial silanization of the surface of the dry adhesive by Chemical Vapour Deposition （CVD） of octadecyltrichlorosilane （OTS）. The partial silanization of the surface of the sample results in a hydrophobic to hy- drophilic gradient across the surface of the dry adhesive. The resulting change in hydrophobicity across the surface of the dry adhesive results in the uphill motion of a droplet of water, which appears to be directly proportional to the area of contact between the droplet and the adhesive. Normal adhesion testing is performed to quantify the effect of the hydrophobic gradient across the surface of the sample. While a variation in adhesion are only minimally affected by the silanization, and the motion strength across the sample is measured, the adhesive properties of the droplet of water doesn＇t cause any loss of adhesion.

关键词： dry adhesion self cleaning hydrophobic silanization polymers PDMS

A probabilistic model to evaluate the effectiveness of main solutions to COVID-19 spreading in university buildings according to proximity and time-based consolidated criteria

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Building Simulation 2021年第6期14卷 1795-1809页

作者： Marco D'Orazio Gabriele Bernardini enrico Quagliarini Department of Construction Civil Engineering and ArchitectureUniversita Politecnica delle Marchevia Brecce Blanche 60131 AnconaItaly

University buildings are one of the most relevant closed environments in which the COVID-19 event clearly pointed out stakeholders'needs toward safety issues,especially because of the possibility of day-to-day presences of the same users(***,teachers)and overcrowding causing long-lasting contacts with possible"infectors".While waiting for the vaccine,as for other public buildings,policy-makers’measures to limit virus outbreaks combine individual’s strategies(facial masks),occupants,capacity and access ***,up to now,no easy-to-apply tools are available for assessing the punctual effectiveness of such *** fill this gap,this work proposes a quick and probabilistic simulation model based on consolidated proximity and exposure-time-based rules for virus transmission confirmed by international health *** building occupancy is defined according to university scheduling,identifying the main"attraction areas"in the building(classrooms,break-areas).Scenarios are defined in terms of occupants’densities and the above-mentioned mitigation *** model is calibrated on experimental data and applied to a relevant university *** demonstrate the model *** particular,it underlines that if such strategies are not combined,the virus spreading can be limited by only using high protection respiratory devices(***3)by almost every *** the contrary,the combination between access control and building capacity limitation can lead to the adoption of lighter protective devices(*** masks),thus improving the feasibility,users'comfort and favorable *** rules to combine acceptable mask filters-occupants,density are thus provided to help stakeholders in organizing users'presences in the building during the pandemic.

关键词： simulation model closed built environment building occupancy crowd models proximity exposure COVID-19

Gas liquid cylindrical cyclone flow regime identification using machine learning combined with experimental mechanism explanation

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Petroleum Science 2023年第1期20卷 540-558页

作者： Zhao-Ming Yang Yu-Xuan He Qi Xiang enrico Zio Li-Min He Xiao-Ming Luo Huai Su Ji Wang Jin-Jun Zhang National Engineering Laboratory for Pipeline Safety/MOE Key Laboratory of Petroleum Engineering/Beijing Key Laboratory of Urban Oil and Gas Distribution Technology China University of Petroleum-Beijing102249BeijingChina MINES ParisTech PSL Research UniversityCRCSophia AntipolisFrance Dipartimento di Energia Politecnico di MilanoVia La Masa 3420156MilanoItaly College of Pipeline and Civil Engineering China University of Petroleum-East China266580QingdaoChina

The flow regimes of GLCC with horizon inlet and a vertical pipe are investigated in experiments,and the velocities and pressure drops data labeled by the corresponding flow regimes are *** with the flow regimes data of other GLCC positions from other literatures in existence,the gas and liquid superficial velocities and pressure drops are used as the input of the machine learning algorithms respectively which are applied to identify the flow *** choosing of input data types takes the availability of data for practical industry fields into consideration,and the twelve machine learning algorithms are chosen from the classical and popular algorithms in the area of classification,including the typical ensemble models,SVM,KNN,Bayesian Model and *** results of flow regimes identification show that gas and liquid superficial velocities are the ideal type of input data for the flow regimes identification by machine *** of the ensemble models can identify the flow regimes of GLCC by gas and liquid velocities with the accuracy of 0.99 and *** the pressure drops as the input of each algorithm,it is not the suitable as gas and liquid velocities,and only XGBoost and Bagging Tree can identify the GLCC flow regimes *** success and confusion of each algorithm are analyzed and explained based on the experimental phenomena of flow regimes evolution processes,the flow regimes map,and the principles of *** applicability and feasibility of each algorithm according to different types of data for GLCC flow regimes identification are proposed.

关键词： Gas liquid cylindrical cyclone Machine learning Flow regimes identification Mechanism explanation Algorithms

Data-driven reliability analysis of Boeing 787 Dreamliner

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Chinese Journal of Aeronautics 2020年第7期33卷 1969-1979页

作者： Guru PANDIAN Michael PECHT enrico ZIO Melinda HODKIEWICZ Center for Advanced Life Cycle Engineering(CALCE) University of MarylandCollege ParkMD 20742USA Department of Energy Politecnico di MilanoItaly Faculty of Engineering and Mathematical Sciences University of Western AustraliaPerthWA 6009Australia

The Boeing 787 Dreamliner,launched in 2011,was presented as a game changer in air *** the aim of producing an efficient,mid-size,wide-body plane,Boeing initiated innovations in product and process design,supply chain operation,and risk ***,there were reliability issues from the start,and the plane was grounded by the *** Aviation Administration(FAA)in 2013,due to safety problems associated with Li-ion battery *** paper chronicles events associated with the aircraft’s initial reliability *** manufacturing,supply chain,and organizational factors that contributed to these problems are assessed based on FAA *** and lessons learned are provided for the benefit of engineers and managers who will be engaged in future complex systems development.

关键词： Boeing 787 Dispatch reliability Dreamliner Lithium-ion battery Reliability Reliability growth