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Application of hepatitis B virus replication mouse model

World Journal of Gastroenterology 2010年第16期16卷 1979-1985页

作者： Gao, Zhan Liu, Feng-Jun Liu, Li Zhou, Tao-You Lei, Jun Xu, Lu Liu, Cong Dai, Jie Chen, en-qiang Tang, Hong W China Hosp Sichuan Univ Ctr Infect Dis Chengdu 610041 Sichuan Prov Peoples R China Sichuan Univ Div Infect Dis State Key Lab Biotherapy Chengdu 610041 Sichuan Prov Peoples R China N Sichuan Med Coll Affiliated Hosp Dept Infect Nanchong 637000 Sichuan Prov Peoples R China

AIM:To evaluate the value of the hepatitis B virus(HBV) replication mouse model with regard to several aspects of the study of HBV ***:To evaluate the HBV replication mouse model in detecting the efficacy of anti-HBV agents,the interferon inducer polyinosinic-polytidylin acid(polyIC) and nucleotide analogues adefovir and entecavir were administered to mice injected with wild type pHBV4.1,and the inhibiting effect of these agents on HBV DNA replication was *** identify the model's value in a replication ability study of HBV drug-resistant mutants and a HBx-minus mutant,telbivudine resistance mutants(rtM204I,ayw subtype),adefovir resistance mutants(rtA181V + rtN236T,ayw subtype) and HBxminus mutants were injected respectively,and their corresponding HBV DNA replication intermediates in mouse liver were ***:Compared with the wild type HBV replication mouse model without antiviral agent treatment,the HBV DNA replication intermediates of the polyICtreated group were decreased 1-fold;while in the entecavir-and adefovir-treated groups,the levels of HBV DNA replication intermediates were inhibited 13.6-fold and 1.4-fold,*** the mouse models injected with telbivudine resistance mutant,adefovir resistance mutant and HBx-minus mutant,HBV DNA replication intermediates could still be detected,but the levels of HBV DNA replication intermediates of these mutants decreased 4.5-fold,5.6-fold and 2.9-fold respectively,compared with the mouse model with wild type HBV ***:The HBV replication mouse model we established was a useful and convenient tool to detect the efficacy of antiviral agents and to study the replication ability of HBV mutants in vivo.

关键词： Hepatitis B virus Antiviral agents Drug resistance Mutants Mouse model

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Changes in the spectrum of gastric polyps in the Chinese population

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World Journal of Gastroenterology 2015年第33期21卷 9758-9764页

作者： Nan-Nan Fan Jing Yang Gang Sun Zhong-Sheng Lu en-qiang Ling Hu Xiang-Dong Wang Yun-Sheng Yang Institute of Digestive Diseases Chinese PLA General Hospital Nankai University School of Medicine

AIM: To evaluate the change in spectrum of gastric polyps in the Chinese population in the past ten ***: A total of 157902 consecutive patients undergoing esophagogastroduodenoscopy(EGD) from 2004 to 2013 in a tertiary hospital were retrospectively reviewed using an EGD *** records of 4043 patients diagnosed with gastric polyps were recalled for *** including demographics,information on polyps such as location,pathological diagnosis,reflux esophagitis and Helicobacter pylori infection were *** focused on epithelial polyps,especially hyperplastic polyps,fundic gland polyps and adenomas,and histological classification of specimens from biopsy and endoscopic polypectomy was performed by professional pathologists,based on the updated *** explore the age distribution of gastric polyps over time,we divided patients with polyps into four groups: A(aged 60 years).Differences in localization,age,and sex distribution of gastric polyps were analyzed by statistical ***: A total of 157902 EGD procedures were performed in ten years at our digestive endoscopy center,of which 4043 cases were diagnosed with gastric polyps confirmed by *** were 2574(63%) female and 1469(37%) male patients with an average age of 54.7 *** overall prevalence of gastric polyps was 2.6%(4043/157902).Our database demonstrated a rising prevalence of gastric polyps over the decade,increasing from 1.0%(80/8025) to 4.70%(828/17787) between 2004 and *** has been a change in the spectrum of gastric polyps with the frequencies of FGPs increasing from 19%(15/80) to 77%(638/828) and hyperplastic polyps decreasing from 65%(52/80) to 15%(123/828).Moreover,data on 1921 polyps in 828 patients diagnosed with gastric polyps in 2013 showed that FGP was the most common type in the current polyp spectrum,making up 81.3%(1562/1921).Location and age distribution of gastri

关键词： Gastric polyps Hyperplastic polyps Fundic gland po

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MiRNA profile in esophageal squamous cell carcinoma:Downregulation of miR-143 and miR-145

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World Journal of Gastroenterology 2011年第1期17卷 79-88页

作者： Bing-Li Wu Li-Yan Xu Ze-Peng Du Lian-Di Liao Hai-Feng Zhang Qiao Huang Guo-qiang Fang en-Min Li Department of Biochemistry and Molecular Biology Medical College of Shantou University Shantou 515041 Guangdong Province China Institute of Oncologic Pathology Medical College of Shantou University Shantou 515041 Guangdong Province China

AIM:To investigate the expression profile of miRNA in esophageal squamous cell carcinoma(ESCC).METHODS:The expression profile of miRNA in ESCC tissues was analyzed by miRNA *** expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction(PCR) using TaqMan *** mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression ***:A set of miRNAs was found to be deregulated in the ESCC tissues,and the expression levels of miR-143 and-145 were significantly decreased in most of the ESCC tissues *** miR-143 and miR-145 expression correlated with tumor invasion *** transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility,however,the protein level of the previously reported target of miR-145,FSCN1,did not show any significant ***:These findings suggest that the deregulation of miRNAs plays an important role in the progression of *** miR-143 and miR-145 might act as anti-oncomirs common to ESCC.

关键词： Esophageal squamous cell carcinoma MicroRNA miR-143 miR-145 Tumor invasion depth

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胰腺囊性肿瘤的超声内镜诊断和治疗

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中国实用内科杂志 2021年第5期41卷 378-381页

作者：令狐恩强李惠凯解放军总医院第一医学中心消化内科医学部北京100853

随着影像学技术的进步,胰腺囊性肿瘤(PCN)的检出率越来越高。不同类型的PCN癌变率不同,治疗方法亦有所区别。其治疗前的精确诊断对治疗策略的选择起着决定性的作用。文章结合笔者的工作实践和文献进展,阐述了EUS及其相关技术在PCN诊断... 详细信息

随着影像学技术的进步,胰腺囊性肿瘤(PCN)的检出率越来越高。不同类型的PCN癌变率不同,治疗方法亦有所区别。其治疗前的精确诊断对治疗策略的选择起着决定性的作用。文章结合笔者的工作实践和文献进展,阐述了EUS及其相关技术在PCN诊断中的作用,并介绍了EUS引导下PCN消融术的历史、适应证和禁忌证、操作方法、疗效判断和随访观察等方面的进展。

关键词：胰腺囊性肿瘤超声内镜诊断治疗

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Establishment of the Method of Immunohistochemistry Assay for the Detection of Scrapie in Chinese Short-Tailed Han Sheep by Monoclonal Antibody

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Agricultural Sciences in China 2008年第12期7卷 1516-1523页

作者： ZHANG Yong-qiang WANG Zhi-liang WU Xiao-dong LIU Yu-tian ZHANG Hai-tao BAO en-dong National Diagnostic Center for Exotic Animal Disease/China Animal Health and Epidemiology Center Qingdao 266032 P.R.China College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 P.R.China

The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. Using the polymerase chain reaction technique, PrP27-30 gene sequence was amplified from Chinese Short-tailed Han sheep genomic DNA. By recombinant DNA technology, the recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained. Then, using standard methodology of myeloma cell fusion, a panel of monoclonal antibodies was generated. With mAbs, scrapie in Chinese Short-tailed Han sheep was detected by immunohistochemistry assay. The recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained and a panel of six hybridoma cell lines secreting specific antibodies to Chinese Short-tailed Han sheep PrP27-30 related to scrapie was obtained with one fusion between myeloma Sp2/0 and spleen ceils from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep. So that the special monoclonal antibody developed in author＇s institute can be used to detect PrP^sc of scrapie in Chinese Short-tailed Han sheep by immunohistochemistry in China.

关键词： Chinese Short-tailed Han sheep PrP27-30 scrapie monoclonal antibody immunohistochemistry assay

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Multivariate predictive model for asymptomatic spontaneous bacterial peritonitis in patients with liver cirrhosis

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World Journal of Gastroenterology 2020年第29期26卷 4316-4326页

作者： Bo Tu Yue-Ning Zhang Jing-Feng Bi Zhe Xu Peng Zhao Lei Shi Xin Zhang Guang Yang en-qiang Qin Department of Infectious Disease the Fifth Medical CenterChinese People's Liberation Army General HospitalBeijing 100039China Center of Hepatology and Gastroenterology Beijing Capital Medical University You’an HospitalBeijing 100039China Department of Clinical and Translational Medicine the Fifth Medical CenterChinese People's Liberation Army General HospitalBeijing 100039China

BACKGROUNDSpontaneous bacterial peritonitis (SBP) is a detrimental infection of the asciticfluid in liver cirrhosis patients, with high mortality and morbidity. Earlydiagnosis and timely antibiotic administration have successfully decreased themortality rate to 20%-25%. However, many patients cannot be diagnosed in theearly stages due to the absence of classical SBP symptoms. Early diagnosis ofasymptomatic SBP remains a great challenge in the *** establish a multivariate predictive model for early diagnosis of asymptomaticSBP using positive microbial cultures from liver cirrhosis patients with *** total of 98 asymptomatic SBP patients and 98 ascites liver cirrhosis patients withnegative microbial cultures were included in the case and control groups,respectively. Multiple linear stepwise regression analysis was performed toidentify potential indicators for asymptomatic SBP diagnosis. The diagnosticperformance of the model was estimated using the receiver operatingcharacteristic *** in the case group were more likely to have advanced disease stages,cirrhosis related-complications, worsened hematology and ascites, and higher mortality. Based on multivariate analysis, the predictive model was as follows: y (P) = 0.018 + 0.312 × MELD (model of end-stage liver disease) + 0.263 × PMN(ascites polymorphonuclear) + 0.184 × N (blood neutrophil percentage) + 0.233 ×HCC (hepatocellular carcinoma) + 0.189 × renal dysfunction. The area under thecurve value of the established model was 0.872, revealing its high diagnosticpotential. The diagnostic sensitivity was 73.5% (72/98), the specificity was 86.7%(85/98), and the diagnostic efficacy was 80.1%.CONCLUSIONOur predictive model is based on the MELD score, polymorphonuclear cells,blood N, hepatocellular carcinoma, and renal dysfunction. This model mayimprove the early diagnosis of asymptomatic SBP.

关键词： Spontaneous bacterial peritonitis Asymptomatic Ascites Multivariate predictive model Liver cirrhosis

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Optical Properties and Thermal Stability of a Cubic Sulfate Rb_(2)Ca_(2)(SO_(4))_(3)

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Chinese Journal of Structural Chemistry 2021年第7期40卷 949-954,841页

作者： ZHONG Xiao-Yu WU en-qiang YANG Sheng-Dong WANG Hao-Hang LIN Xiao-Xin SHen Yao-Guo College of Physics&Electronic Information engineering Minjiang UniversityFuzhou 350108China

In this paper,a non-centrosymmetric compound of Rb_(2)Ca_(2)(SO_(4))_(3)has been synthesized by a high temperature solid-state reaction and high temperature melting ***-crystal X-ray diffraction analysis shows that Rb_(2)Ca_(2)(SO_(4))_(3)crystallizes in the cubic space group of P2_(1)3,and its cell parameters are a=b=c=10.5569(6)Å,Z=4 and V=1176.55(12)Å^(3),*** the crystal structure,SO4 tetrahedra and CaO6 octahedra are connected with each other by a corner-sharing mode to construct the three-dimensional framework of Rb_(2)Ca_(2)(SO_(4))_(3).Optical measurements show that the title compound has a short ultraviolet absorption edge and a moderate second-harmonic generation *** optical origin is illustrated by the electron band structure *** addition,thermal stability is also studied by virtue of differential thermal/thermogravimetric analysis and powder XRD technique.

关键词： crystal structure nonlinear optics solid-state reaction sulfate

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煤矿水害应急救援预案探讨

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煤炭学报 2006年第4期31卷 409-413页

作者：武强管恩太中国矿业大学(北京)水资源与环境研究所北京100083 郑州煤业集团河南郑州450032

煤矿水害应急救援预案的制订对有效遏制煤矿水害事故频发和减少水害事故造成的人员伤亡和财产损失具有极其重要的意义.“预防为主、防救结合”是我国煤矿水害应急救援预案制订的“八字”基本原则.煤矿水害应急救援预案应包括水害发生前... 详细信息

煤矿水害应急救援预案的制订对有效遏制煤矿水害事故频发和减少水害事故造成的人员伤亡和财产损失具有极其重要的意义.“预防为主、防救结合”是我国煤矿水害应急救援预案制订的“八字”基本原则.煤矿水害应急救援预案应包括水害发生前的事故预防和水害发生后的事故抢险救援两大部分.事故预防主要包括在事故发生前的扎实的基础防治水工作和透(突)水危险预兆的有效辨识两部分;而事故抢险救援主要包括在事故发生后启用预定的紧急处置程序和现场开展的自救互救方法两部分.

关键词：水害应急救援预案防治水危险预兆辨识紧急处置程序自救互救

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Treatment of gastric varices by endoscopic sclerotherapy using butyl cyanoacrylate:10 years' experience of 635 cases

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Chinese Medical Journal 2007年第23期120卷 2081-2085页

作者： CHenG Liu-fang WANG Zhi-qiang LI Chang-zheng CAI Feng-chun HUANG Qi-yang linghu en-qiang LI Wen CHAI Guo-jun SUN Guo-hui MAO Yong-ping WANG Yan-mei LI Jing GAO Ping FAN Tie-yan Department of Gastroenterology and Hepatology General Hospital of Chinese People＇s Liberation Army Beijing 100853 China

Background Gastric varices （GV） are life-threatening for patients with portal hypertension. endoscopic injection with butyl cyanoacrylate （BC）, the mainstay of the therapy for GV, has been reported to be effective for hemostasis of bleeding varices, but its efficacy in the obliteration of GV and impact on the survival of patients still needs clarification. Here we summarized our experience of 10 years＇ practice to evaluate the efficacy and safety of endoscopic therapy using BC for GV patients. Methods From January 1997 to April 2006, GV cases treated with endoscopic injection using BC were collected. The ＂sandwich method＂ and the ＂modified sandwich method＂ were used to inject BC intravascularly. Retrograde analysis was made on the data of treatment and follow-up. Results A total of 635 GV cases treated with endoscopic injection using BC were collected, most of them （90.2%） suffered from post-hepatitis cirrhosis. Emergency hemostasis was achieved in 139 out of 146 sessions （95.2%）. Complications occurred in 32 cases （5.2%）, including hemorrhage due to early expulsion of tissue glue （3.1%）, septicemia （1%） and ectopic thrombosis （0.5%）, such as spleen infarction. endoscopic follow-up in 503 patients showed complete disappearance （76.9%）, collapse （17.3%） or remnants （5.8%） of gastric varices. A total of 550 patients were followed up clinically for 3 to 115 months. Of these patients, 44 had recurrent bleeding （8.0%） and 44 died from hepatic failure, recurrent bleeding, hepatic carcinoma or other causes. The longest survival was 115 months, with a median survival of 25 months. Survival rates at 1,2, 3, 4 and 5 year were 95%, 92%, 90%, 83% and 81%, respectively. Conclusions endoscopic sclerotherapy with BC is effective for the hemostasis of bleeding GV, as well as obliteration of GV which contributes to less rebleeding and better survival. The modified sandwich method may be useful to minimize ectopic embolism, which we speculated to result from excess iodized oil.

关键词： gastric varices endoscopic sclerotherapy butyl cyanoacrylate survival

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Towards Exploring Large Molecular Space:An Efficient Chemical Genetic Algorithm

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Journal of Computer Science & Technology 2022年第6期37卷 1464-1477页

作者： Jian-Fu Zhu Zhong-Kai Hao Qi Liu Yu Yin Cheng-qiang Lu Zhen-Ya Huang en-Hong Chen Anhui Province Key Laboratory of Big Data Analysis and Application School of Computer Science and TechnologyUniversity of Science and Technology of ChinaHefei 230026China

Generating molecules with desired properties is an important task in chemistry and *** efficient method may have a positive impact on finding drugs to treat diseases like *** mining and artificial intelligence may be good ways to find an efficient ***,both the generative models based on deep learning and the work based on genetic algorithms have made some progress in generating molecules and optimizing the molecule’s ***,existing methods have defects in the experimental evaluation *** methods also need to be improved in efficiency and *** solve these problems,we propose a method named the Chemical Genetic Algorithm for Large Molecular Space(CALM).Specifically,CALM employs a scalable and efficient molecular representation called molecular *** we design corresponding crossover,mutation,and mask operators inspired by domain knowledge and previous *** apply our genetic algorithm to several tasks related to molecular property optimization and constraint molecular *** results of these tasks show that our approach outperforms the other state-of-the-art deep learning and genetic algorithm methods,where the z tests performed on the results of several experiments show that our method is more than 99%likely to be *** the same time,based on the experimental results,we point out the defects in the experimental evaluation standard which affects the fair evaluation of all previous *** these defects helps to objectively evaluate the performance of all work.

关键词： data mining molecular generation genetic algorithm drug discovery artificial intelligence

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