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Host Factors Alter Effects of Angiopoietin-Like Protein 8 on Glucose Homeostasis in Diabetic mice

Journal of Diabetes mellitus 2016年第4期6卷 277-290页

作者： Sichen Liu emily m. Smith Timothy H. King Lindsey Glenn michelle Trevino So Hyun Park Yui machida Ciriaco Villaflor Wojciech Grzesik margaret A. morris Yumi Imai Jerry L. Nadler Eastern Virginia medical School Department of Internal Medicine Strelitz Diabetes Center Norfolk VA USA Department of Internal medicine Fraternal Order of Eagles Diabetes Research Center The University of Iowa Carver College of Medicine Iowa City IA USA

Recovery of functional beta cell mass offers a biological cure for type 1 diabetes. However, beta cell mass is difficult to regain once lost since the proliferation rate of beta cells after youth is very low. Angiopoietin like-protein 8 (ANGPTL8), a peptide that has a role in the regulation of lipoprotein lipase activity, was reported to increase beta cell proliferation in mice in 2013. Subsequent studies of human ANGPTL8 for short term (3 to 8 days) in non-diabetic mice showed little or no increase in beta cell proliferation. Here, we examined the effect of ANGPTL8 on glucose homeostasis in models that have not been examined previously. We expressed mouse ANGPTL8 using adenovirus in 2 mouse models of diabetes (streptozotocin and Non-Obese Diabetic (NOD) mice) over 2 weeks. Also, we tested ANGPTL8 in NOD mice deficient in leukocyte 12-lipoxygenase (12LO), an enzyme that contributes to insulitis and loss of beta cell function in NOD, in an effort to determine whether 12LO deficiency alters the response to ANGPTL8. Adenovirus-mediated expression of ANGPTL8 lowered blood glucose levels in streptozotocin treated mice without an increase in beta cell proliferation or serum insulin concentration. While ANGPTL8 did not reverse hyperglycemia in overtly hyperglycemic NOD mice or alter glucose homeostasis of non-diabetic NOD mice, ANGPTL8 reduced blood glucose levels in 12LOKO NOD mice. However, the lower glucose levels in 12LOKO NOD were not associated with higher serum insulin levels or beta cell proliferation. In summary, while mouse ANGPTL8 does not increase beta cell proliferation in NOD mice or streptozotocin treated mice in agreement with studies in non-diabetic mice, it lowers blood glucose levels in multiple low-dose streptozotocin induced diabetes and 12LO deficiency indicating that host factors influence the impact of ANGPTL8 on glucose homeostasis.

关键词： Angiopoietin-Like Protein 8 Type 1 Diabetes NOD 12-Lipoxygenase Beta Cells Glucose Homeostasis

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A new device for deep cervical artificial insemination in gilts reduces the number of sperm per dose without impairing final reproductive performance

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Journal of Animal Science and Biotechnology 2019年第2期10卷 402-409页

作者： Pedro J.Llamas-López Rebeca López-úbeda Gustavo López emily Antinoja Francisco A.García-Vázquez Department of Physiology Faculty of VeterinaryCampus Mare NostrumUniversity of Murcia30100 MurciaSpain Department of Cell Biology and Histology Faculty of MedicineCampus Mare NostrumUniversity of Murcia30100 MurciaSpain Institute for Biomedical Research of murcia(ImIB-Arrixaca) MurciaSpain Cherkizovo Group LipetskRussia.

Background: The aim of this study was to evaluate the reproductive performance of a new artificial insemination(AI) device specifically designed for gilts(Deep cervical AI, Dp-CAI) by means of which the sperm is deposited deeply in the cervix(8 cm more cranial than in traditional cervical insemination-CAI). New AI techniques have arisen in recent decades in the porcine industry, such as post-cervical artificial insemination(PCAI), which involves depositing the sperm in the body of the uterus [through a catheter(outer tube)-cannula(inner tube)] rather than by CAI. Although the PCAI method has been successfully applied in farm conditions to reduce sperm doses without impairing the reproductive performance, this technique has limitations in gilts mainly because of the difficulty involved in introducing the inner cannula through the cranial part of the cervix. For this reason, the Dp-CAI method described herein may be considered as an alternative to CAI and PCAI methods in ***: Gilts were divided in two experimental groups: 1) Dp-CAI: gilts(n = 1166) inseminated using 1.5 × 109 sperm/45 m L;2) CAI(as a control group): gilts(n = 130) inseminated using 2.5 × 10~9 sperm/85 mL. The Dp-CAI method was successfully applied in 88.90% of the gilts, with no differences detected between gilts with 1 or 2 previous oestrus cycles, although the catheter could be introduced more deeply in 2 oestrus gilts(P mination device that could not be introduced increased(at the moment of insemination), so the success rate of the Dp-CAI device fell, as did the total number of piglets born. When the reproductive output in CAI and Dp-CAI was compared, none of the parameters analysed [pregnancy and farrowing rates(%), and number of piglets born(total and live)] showed significant ***: The use of the Dp-CAI technique provides a new AI method as an alternative to CAI and PCAI for pigs. The device, especially designed for gilts, was used w

关键词： Cervix Intrauterine Nulliparous Porcine Post-cervical insemination

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Enhancing maize Grain Yield in Acid Soils of Western Kenya Using Aluminium Tolerant Germplasm

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Journal of Agricultural Science and Technology(A) 2013年第1期3卷 33-46页

作者： Ouma Evans Ligeyo Dickson matonyei Thomas Agalo Joyce Were Beatrice Too emily OnkwareAugustino Gudu Samuel Kisinyo Peter Philip Nyangweso Department of Biological Sciences Moi University P.O. Box 3900-30100 Eldoret Kenya. Kenya Agricultural Research Institute (KARI) Kitale Centre P.O. Box 460-30200 Kenya Department of Biological Sciences Chepkoilel University College P.O. Box 1125-30100 Eldoret Kenya Department of Agronomy Rongo University College P.O. Box 103-40404 Rongo-Kenya

maize （Zea mays L.） is one of the world＇s most important cereals and is a staple food for many people in developing countries. However, in acid soils （pH 〈 5.5）, its productivity is limited by aluminium （AI） toxicity, besides other factors. The objectives of this study were to： develop AI tolerant maize inbred lines for a maize breeding program in Kenya, develop single cross hybrids （SCHs） from some of the tolerant inbred lines and determine AI tolerance levels of the SCHs. One hundred and seventy five inbreds and 49 SCHs were developed and screened in nutrient culture containing 0 or 222μm using Relative Net Root Growth （RNRG）, hematoxylin staining （HS） and under AI saturated field conditions （44%-45.6%） at Sega and Chepkoilel. Seedling root growth was inhibited in 95% of the inbreds. F hybrids obtained from inbreds varying in A1 tolerance, exhibited tolerance equal to or greater than that of the more tolerant parent indicating a positive transgressive inheritance to AI toxicity. Fifty eight percent of the F SCHs were heterotic for tolerance to AI toxicity. AI tolerance estimated by RNRG was well correlated to that of HS （r2 = 0.88, P 〈 0.005） but minimally correlated with the field estimates （r2 = 0.24-0.35）, implying that RNRG can predict field selection under AI toxic soils by between 24% and 35%. Plant breeders should therefore employ both approaches in selecting cultivars under AI stress. This study has developed and identified A1 tolerant inbreds and SCHs for use in the acid soils of Kenya and similar regions.

关键词： maize inbred lines hybrids heterosis aluminium toxicity acid soils.

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Stressing over the Complexities of multiple Stressors in marine and Estuarine Systems

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Ocean-Land-Atmosphere Research 2022年第1期1卷 27-53页

作者： Patricia m.Glibert Wei-Jun Cai emily R.Hall ming Li Kevan L.main Kenneth A.Rose Jeremy m.Testa Nayani K.Vidyarathna University of maryland Center for Environmental Science Horn Point LaboratoryPO Box 775CambridgeUSA School of marine Science and Policy University of Delaware111 Robinson HallNewarkDE 19716USA mote marine Laboratory 1600 Ken Thompson ParkwaySarasotaFL 34236USA University of maryland Center for Environmental Science Chesapeake Biological LaboratoryPO Box 38SolomonsUSA

Aquatic ecosystems are increasingly threatened by multiple human-induced stressors associated with climate and anthropogenic changes,including warming,nutrient pollution,harmful algal blooms,hypoxia,and changes in CO_(2) and *** stressors may affect systems additively and synergistically but may also counteract each *** resultant ecosystem changes occur rapidly,affecting both biotic and abiotic components and their ***,the complexity of interactions increases as one ascends the food web due to differing sensitivities and exposures among life stages and associated species interactions,such as competition and *** is also a need to further understand nontraditional food web interactions,such as mixotrophy,which is the ability to combine photosynthesis and feeding by a single *** complexity of these interactions and nontraditional food webs presents challenges to ecosystem modeling and *** ecological models to understand multistressor effects is further challenged by the lack of sufficient data on the effects of interactive stressors across different trophic levels and the substantial variability in climate changes on regional *** obtain data on a broad suite of interactions,a nested set of experiments can be ***,coupled,multitrophic level models will provide the flexibility to explore the additive,amplified,propagated,antagonistic,and/or reduced effects that can emerge from the interactions of multiple ***,the stressors associated with eutrophication and climate change are reviewed,and then example systems from around the world are used to illustrate their complexity and how model scenarios can be used to examine potential future changes.

关键词： additive marine complexity

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Differential expression of pancreatic protein and chemosensing receptor m RNAs in NKCC1-null intestine

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World Journal of Gastrointestinal Pathophysiology 2016年第1期7卷 138-149页

作者： emily m Bradford Kanimozhi Vairamani Gary E Shull Department of Internal medicine Division of GastroenterologyUniversity of Kentucky Department of molecular Genetics Biochemistry and MicrobiologyUniversity of Cincinnati College of Medicine

AIm: To investigate the intestinal functions of the NKCC1 Na^+-K^+-2Cl cotransporter(SLC12a2 gene), differential m RNA expression changes in NKCC1-null intestine were ***: microarray analysis of m RNA from intestines of adult wild-type mice and gene-targeted NKCC1-null mice(n = 6 of each genotype) was performed to identify patterns of differential gene expression changes. Differential expression patterns were further examined by Gene Ontology analysis using the online Gorilla program, and expression changes of selected genes were verified using northern blot analysis and quantitative real time-polymerase chain reaction. Histological staining and immunofluorescence were performed to identify cell types in which upregulated pancreatic digestive enzymes were ***: Genes typically associated with pancreatic function were upregulated. These included lipase, amylase, elastase, and serine proteases indicative of pancreatic exocrine function, as well as insulin and regenerating islet genes, representative of endocrine function. Northern blot analysis and immunohistochemistry showed that differential expression of exocrine pancreas m RNAs was specific to the duodenum and localized to a subset of goblet cells. In addition, a major pattern of changes involving differential expression of olfactory receptors that function in chemical sensing, as well as other chemosensing G-protein coupled receptors, was observed. These changes in chemosensory receptor expression may be related to the failure of intestinal function and dependency on parenteral nutrition observed in humans with SLC12a2 mutations. CONCLUSION: The results suggest that loss of NKCC1 affects not only secretion, but also goblet cell function and chemosensing of intestinal contents via G-protein coupled chemosensory receptors.

关键词： SLC12a2 Chemosensory Chemosensitivity Gastrointestinal Dyspepsia

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WONCA研究论文摘要汇编——与预防保健服务收入相关的初级医疗属性:一项全国性研究

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中国全科医学 2015年第34期18卷 4209-4209页

作者： emily C Anthony F Peter m 本刊编辑部

背景初级医疗属性(PCAs)是以患者为中心的医疗之家的组成部分,可以增加预防保健服务收入,但目前与其相关的全国性研究较少。方法从2007—2010年美国医药费用委员会的调查数据中,选取成年人自我报告数据(n=50 457),进行横断面调整。以个... 详细信息

背景初级医疗属性(PCAs)是以患者为中心的医疗之家的组成部分,可以增加预防保健服务收入,但目前与其相关的全国性研究较少。方法从2007—2010年美国医药费用委员会的调查数据中,选取成年人自我报告数据(n=50 457),进行横断面调整。以个人为单位进行PCAs评分,其主要内容包括综合性(对新出现或现有问题的治疗来源、预防保健及转诊情况)、以患者为中心性(决策分享)、加强随访性(夜间和周末时间)。预防保健措施包括乳腺X线摄影、流感疫苗接种、年度体检、结直肠癌筛查、前列腺特异性抗原检测及胆固醇监测。结果 PCAs评分与各项预防保健措施收入间均呈正相关。结直肠癌筛查(18.5%)和前列腺特异性抗原检测(20.7%)的PCAs四分位数增长最高。PCAs中的综合性和以患者为中心性均与各项预防保健措施收入呈正相关;加强渠道性与年度体检收入呈负相关〔OR(95%CI)=0.83(0.77,0.91)〕。结论在全国代表性样本中,除加强渠道性外,其他关键PCAs均可增加预防保健收入。该结论有利于基层医疗机构实现预防保健服务交付量的最大化。

关键词：预防保健 WONCA 医疗收入财政管理属性

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静电场驱动下液体薄膜的几何形状

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应用数学和力学 2011年第8期32卷 973-980页

作者： E.m.田 T.P.斯沃博德内 J.D.菲利普斯莱特州立大学数学与统计学院

利用六边形-俯视图的弱非线性稳定性分析和数值仿真,在电场作用下,研究高分子薄膜表面静态模式的发展过程.在无限空间域上,空间和高分子薄膜之间的界面,由薄膜方程给出其随时间的演变,综合考虑了电力的驱动和表面张力的传播.非线性界面... 详细信息

利用六边形-俯视图的弱非线性稳定性分析和数值仿真,在电场作用下,研究高分子薄膜表面静态模式的发展过程.在无限空间域上,空间和高分子薄膜之间的界面,由薄膜方程给出其随时间的演变,综合考虑了电力的驱动和表面张力的传播.非线性界面的增长包括:波幅方程的增长,以及在准对规律方向上,一维结构的叠合.模式的选择由亚临界不稳定性机理确定,高分子薄膜的相对厚度在其中起着决定性的作用.

关键词：薄膜模式的形成电流体动力学不稳定性

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Perceptions of the osteopathic profession in New York City’s Chinese Communities

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Family medicine and Community Health 2020年第1期8卷 24-30页

作者： Justin Chin Sarah Li Gregory Yim YaQun Arlene Zhou Peter Justin Wan emily R Dube mikhail Volokitin Sonu Sahni mark A Terrell Christine m Lomiguen Primary Care Touro College of Osteopathic MedicineNew York CityNew YorkUSA Pathology New York University School of MedicineNew York CityNew YorkUSA Osteopathic manipulative medicine Touro College of Osteopathic MedicineNew York CityNew YorkUSA Research medicine New York Institute of Technology College of Osteopathic MedicineOld WestburyNew YorkUSA medical Education Lake Erie College of Osteopathic MedicineEriePennsylvaniaUSA Pathology Lake Erie College of Osteopathic MedicineEriePennsylvaniaUSA

Objective The purpose of this study was to assess knowledge of and barriers to osteopathic medicine in Chinese immigrant communities in New York City(NYC).Design A cross-sectional study was designed in which a culturally appropriate survey in Chinese and English versions was administered anonymously to measure immigrant perceptions and knowledge of osteopathic *** Data collection occurred in the municipal delineations for the Chinatown neighbourhood within the New York,New York borough of *** Community members were selected using convenience sampling from high-density areas to *** gathered from the survey included demographics,education level,healthcare habits and knowledge of the osteopathic *** 120 surveys were conducted with 68 males and 52 females,with an average age=*** in the age range of 18-29 years,those with fluent English-language proficiency,and participants with graduate-level education status demonstrated a higher proportion of knowledge of osteopathic manipulative medicine and osteopathic physicians(doctors of osteopathic medicine)among the study *** Compared with research on the general US population,a general lack of knowledge of osteopathic medicine exists within NYC’s Chinese immigrant *** this difference may be ascribed to linguistics and ethnosociological factors,greater outreach and education is needed in urban minority communities to make immigrants aware of all healthcare resources available during the current shortage of US primary care physicians.

关键词： education profession sectional

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呼啸山庄英汉对照

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丛书名：浓咖啡双语经典丛书

2005年

作者：（英）艾米莉·勃朗特（emily Bronte）田元译

讲述了呼啸山庄的恩萧家族和画眉农庄的林顿家族之间的故事。西斯克利夫是老恩萧收养的孤儿，遭到老恩萧的儿子辛德雷的仇视，由于地位的差异无法与老恩萧的女儿凯茜结合。西斯克利夫愤然出走，发誓报复，最后虽然达到了复仇的愿望，却... 详细信息

ISBN: (数字)750011317X

ISBN: (纸本)750011317X

讲述了呼啸山庄的恩萧家族和画眉农庄的林顿家族之间的故事。西斯克利夫是老恩萧收养的孤儿，遭到老恩萧的儿子辛德雷的仇视，由于地位的差异无法与老恩萧的女儿凯茜结合。西斯克利夫愤然出走，发誓报复，最后虽然达到了复仇的愿望，却并不快乐，在一个风雨之夜结束了自己的生命。

关键词：长篇小说英语近代英国汉语

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The Genetics of Speciation： Considering Early-Acting Isolation, Hybrid Evolution, and Epigenetic mechanisms

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Current Zoology 2013年第5期59卷 654-657页

作者： Amber m. RICE, Editor Lehigh University Department of Biological Sciences Bethlehem PA 18015 USA amber.rice@Lehigh.edu

Since Darwin＇s time, evolutionary biologists have sought to understand how species arise. The study of speciation remains a focus of evolutionary biology, and since the early 204 century, characterizing the genetics underlying this process has also become a central goal. much research into the genetics of speciation has cen- tered on identifying the genes underlying reproductive isolating mechanisms, or ＇speciation genes＇（see Nosil and Schluter, 2011 for a discussion of this term）. With information about such genes available from many sys- tems, general questions about how speciation proceeds may be addressed： How many genes contribute to speciation？ Is reproductive isolation more commonly the result of divergence in protein-coding regions or regulatory regions？ Is reproductive isolation more fre- quently the result of natural selection or genetic drift？（coyne and Orr, 2004; Nosil and Schluter, 2011）. Given recent advances in technology, such questions have be- come accessible in systems that were previously intrac- table for studying the genetics of speciation. moreover, with this increase in the accessibility of genetic analyses for non-model systems, a broader set of questions about the genetics of speciation can now be addressed.

关键词：遗传学特性生物学形态进化遗传机制基因漂移物种形成混合表观

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