检索结果-南通市图书馆

Efficiency in hydrocarbon degradation and biosurfactant production by Joostella sp.A8 when grown in pure culture and consortia

引用

Journal of Environmental Sciences 2018年第5期30卷 115-126页

作者： Carmen Rizzo Alessandro Ciro Rappazzo Luigi Michaud emilio de domenico Carlos Rochera Antonio Camacho Angelina Lo Giudice department of Chemical BiologicalPharmaceutical and Environmental Sciences(ChiBioFarAm)University of MessinaViale Ferdinando Stagno d'Alcontres 3198166 MessinaItaly Institute for the Coastal Marine Environment National Research Council(IAMC-CNR)Spianata San Raineri 8698122 MessinaItaly Cavanilles Institute for Biodiversity and Evolutionary Biology University of ValenciaCarrer del Catedratic Jose Beltran Martinez 246980 PaternaValenciaSpain Regional Centre for Water Studies(CREA) University of Castilla-La ManchaC/Altagracia 5013071 Ciudad RealSpain

Joostella strains are emerging candidates for biosurfactant production. Here such ability was analyzed for Joostella strain A8 in comparison with Alcanivorax strain A53 and Pseudomonas strain A6, all previously isolated from hydrocarbon enrichment cultures made of polychaete homogenates. In pure cultures Joostella sp. A8 showed the highest stable emulsion percentage（78.33%）, hydrophobicity rate（62.67%）, and an optimal surface tension reduction during growth in mineral medium supplemented with diesel oil（reduction of about 12 mN/m）, thus proving to be highly competitive with Alcanivorax and Pseudomonas strains. During growth in pure culture different level of biodegradation were detected for Alcanivorax strain A53（52.7%）,Pseudomonas strain A6（38.2%） and Joostella strain A8（26.8%）. When growing in consortia,isolates achieved similar abundance values, with the best efficiency that was observed for the Joostella-Pseudomonas co-culture. Gas-chromatographic analysis revealed an increase in the biodegradation efficiency in co-cultures（about 90%）, suggesting that the contemporary action of different bacterial species could improve the process. Results were useful to compare the efficiencies of well-known biosurfactant producers（i.e. Pseudomonas and Alcanivorax representatives） with a still unknown biosurfactant producer, i.e. Joostella, and to confirm them as optimal biosurfactant-producing candidates.

关键词： Joostella Biosurfactants Co-cultures Hydrocarbon degradation Biodegradation efficiency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mucosal adhesion and anti-inflammatory effects of Lactobacillus rhamnosus GG in the human colonic mucosa: A proof-of-concept study

引用

World Journal of Gastroenterology 2018年第41期24卷 4652-4662页

作者： Cristiano Pagnini Vito domenico Corleto Michela Martorelli Claudio Lanini Giancarlo D'Ambra emilio Di Giulio Gianfranco delle Fave department of Digestive and Liver Disease School of Medicine and PsychologyS.Andrea HospitalSapienza University

AIM To investigate the adhesion and anti-inflammatory effects of Lactobacil us rhamnosus GG (LGG) in the colonic mucosa of healthy and ulcerative colitis (UC) patients, both in vivo and ex vivo in an organ culture *** For the ex vivo experiment, a total of 98 patients (68 UC patients and 30 normal subjects) were included. Endoscopic biopsies were collected and incubated with and without LGG or LGG-conditioned media to evaluate the mucosal adhesion and anti-inflammatory effects [reduction of tumor necrosis factor alpha (TNFα) and interleukin (IL)-17 expression] of the bacteria, and extraction of DNA and RNA for quantification by real-time (RT)-PCR occurred after the incubation. A dose-response study was performed by incubating biopsies at "regular", double and 5 times higher doses of LGG. For the in vivo experiment, a total of 42 patients (20 UC patients and 22 normal controls) were included. Biopsies were taken from the colons of normal subjects who consumed a commercial formulation of LGG for 7 d prior to the colonoscopy,and the adhesion of the bacteria to the colonic mucosa was evaluated by RT-PCR and compared with that of control biopsies from patients who did not consume the formulation. LGG adhesion and TNFα and IL-17 expression were compared between UC patients who consumed a regular or double dose of LGG supplementation prior to *** In the ex vivo experiment, LGG showed consistent adhesion to the distal and proximal colon in normal subjects and UC patients, with a trend towards higher concentrations in the distal colon, and in UC patients, adhesion was similar in biopsies with active and quiescent inflammation. In addition, bioptic samples from UC patients incubated with LGG conditioned media (CM) showed reduced expression of TNFα and IL-17 compared with the corresponding expression in controls (P < 0.05). Incubation with a double dose of LGG increased mucosal adhesion and the anti-inflammatory effects (P < 0.05). In the in vivo expe

关键词： Lactobacillus rhamnosus GG Ulcerative colitis Probiotic Adhesion Cytokines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficacy of Prucalopride in bowel cleansing before colonoscopy:Results of a pilot study

引用

World Journal of Gastrointestinal Endoscopy 2017年第11期9卷 558-560页

作者： Vito domenico Corleto Giulio Antonelli Chiara Coluccio Lucia D'Alba emilio di Giulio department of Digestive Endoscopy School of Medicine and Psychology 'Sapienza' University of Rome Sant’ Andrea Hospital Gastroenterology and Digestive Endoscopy San Giovanni-Addolorata Hospital

Colonoscopy is a crucial diagnostic instrument for colorectal cancer screening and an adequate bowel preparation is definitely decisive for the success of the procedure. Especially in elderly patients, bowel cleansing is considered a big issue, because it is often poorly tolerated for many reasons(like inability to swallow large volume of liquids or unlikable taste); this can cause a suboptimal preparation that may lead to miss a neoplastic lesion. There is relatively little data about how to improve preparation tolerability. The purpose of our pilot study was to analyze the effect of prucalopride(Resolor~?), a highly selective serotonin 5HT4 receptor agonist used for chronic constipation for its ability to stimulate gastrointestinal peristalsis, undertaken the day before colonoscopy, followed by half volume of polyethylene glycol solution. We found that this can be a good and safe method to achieve an adequate and better-tolerated colon cleansing.

关键词：清洁的肠 Colonoscopy Prucalopride 屏蔽 Colorectal 癌症

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：