Efficiency in hydrocarbon degradation and biosurfactant production by Joostella sp.A8 when grown in pure culture and consortia

作     者：Carmen Rizzo Alessandro Ciro Rappazzo Luigi Michaud Emilio De Domenico Carlos Rochera Antonio Camacho Angelina Lo Giudice 

作者机构：Department of ChemicalBiologicalPharmaceutical and Environmental Sciences(ChiBioFarAm)University of MessinaViale Ferdinando Stagno d'Alcontres 3198166 MessinaItaly Institute for the Coastal Marine EnvironmentNational Research Council(IAMC-CNR)Spianata San Raineri 8698122 MessinaItaly Cavanilles Institute for Biodiversity and Evolutionary BiologyUniversity of ValenciaCarrer del Catedratic Jose Beltran Martinez 246980 PaternaValenciaSpain Regional Centre for Water Studies(CREA)University of Castilla-La ManchaC/Altagracia 5013071 Ciudad RealSpain 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2018年第30卷第5期

页      面：115-126页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the Doctoral School in“Scienze Ambientali:Ambiente Marino e Risorse”of theUniversity of Messina Italy 

主　　题：Joostella Biosurfactants Co-cultures Hydrocarbon degradation Biodegradation efficiency 

摘      要：Joostella strains are emerging candidates for biosurfactant production. Here such ability was analyzed for Joostella strain A8 in comparison with Alcanivorax strain A53 and Pseudomonas strain A6, all previously isolated from hydrocarbon enrichment cultures made of polychaete homogenates. In pure cultures Joostella sp. A8 showed the highest stable emulsion percentage（78.33%）, hydrophobicity rate（62.67%）, and an optimal surface tension reduction during growth in mineral medium supplemented with diesel oil（reduction of about 12 mN/m）, thus proving to be highly competitive with Alcanivorax and Pseudomonas strains. During growth in pure culture different level of biodegradation were detected for Alcanivorax strain A53（52.7%）,Pseudomonas strain A6（38.2%） and Joostella strain A8（26.8%）. When growing in consortia,isolates achieved similar abundance values, with the best efficiency that was observed for the Joostella-Pseudomonas co-culture. Gas-chromatographic analysis revealed an increase in the biodegradation efficiency in co-cultures（about 90%）, suggesting that the contemporary action of different bacterial species could improve the process. Results were useful to compare the efficiencies of well-known biosurfactant producers（i.e. Pseudomonas and Alcanivorax representatives） with a still unknown biosurfactant producer, i.e. Joostella, and to confirm them as optimal biosurfactant-producing candidates.