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Analysis of the Lipolytic Potential of Filamentous Fungi Isolated from Some Plants and Soil Samples in Minas Gerais, Brazil

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Advances in Bioscience and Biotechnology 2020年第11期11卷 475-487页

作者： Paula V. D. Spencer Tatiana P. Costa Mirian J. Souza nísia A. V. D. Pinto David L. nelson Vivian M. Benassi Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil Faculdade de Ciências Biológicas e da Saúde Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil Programa de Pós-Graduacao em Biocombustíveis Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil Instituto de Ciência e Tecnologia Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil

Microorganisms have the ability to produce several metabolites, which are widely used in biotechnological processes, including the biological catalysts called enzymes. Among these enzymes, lipases are favored because they perform various catalytic reactions such as hydrolysis, esterification, interesterification and transesterification. This work sought to isolate filamentous fungi from samples collected in Diamantina, Minas Gerais, Brazil, analyze their macroscopic morphological characteristics, determine the effect of temperature on their growth, and verify which organisms are potential lipase producers. From four collection sites, nine fungi were isolated from the leaves and soil of the jabuticaba (Plinia cauliflora) tree and three fungi from the Andu bean leaves (Cajanus cajan). The macroscopic morphological characteristics of the microorganisms were analyzed. For thermophilic analysis, the twelve isolated fungi and eight obtained from the laboratory bank were cultivated in a solid Potato-Dextrose-Agar medium from 30°C to 50°C, with intervals of 5°C. The growth rate per hour after 48 hours of development was calculated. For the selection of filamentous lipase-producing fungi, the 20 fungi were cultivated in solid BDA medium at 30n style="font-family:Verdana;">°C for 96 hours, and the enzyme index was calculated. It was found that, of the 20 microorganisms, 95% developed at 30°C, and the highest growth rates were those of fungi 3.2TA, PJ8 and PJ7. At 35°C, 70% of the fungi developed, and the highest growth rates were those of fungi 3.2TA, MB2.2 and P3. At 40°C, 40% of the fungi developed, and the highest growth rates were those of fungi 3.2TA, PJ6 and PJ8. At

关键词： Enzymes Bioprospecting Biotechnology

水力旋流器的研究现状及其在石油工业中的应用前景

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过滤与分离 1999年第3期9卷 1-4页

作者：倪玲英石油大学石油工程系山东东营257062

介绍了油水分离水力旋流器在国内外的研究现状和发展趋势，分析了水力旋流器在油田油水分离系统中的应用前景，对井下油水分离新技术的工作原理。

关键词：水力旋流器油水分离研究现状应用前景

应用组织多普勒成像评估慢性心力衰竭患者的运动耐量及心功能

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世界核心医学期刊文摘（心脏病学分册） 2005年第2期1卷 36-37页

作者： Witte K.K.A. nikitin n.P. De Silva R. 郭宁 Academic Cardiology Castle Hill Hospital Castle Road Cott inghom Hull HU16 5JQ United Kingdom Dr.

Objective: To examine the relation between longitudinal left ventricular funct ion assessed by tissue Doppler imaging (TDi) and exercise capacity in heart fail ure. Subjects: 153 patients with chronic heart failure from left ventricular sys tolic dysfunction (ejection fractionnd 87 age and sex matched controls. Methods: Echocardiography was used to measure conventional indices of left vent ricular systolic function. TDi was used to assess left and right ventricular lon gitudinal function by measuring mitral and lateral tricuspid annular velocities during the cardiac cycle. Velocities measured at each point were the systolic pe ak (Sm) and the diastolic troughs(em and Am), corresponding to passive and activ e (atrial) left ventricular filling. Each patient also underwent treadmill exerc ise testing with metabolic gas exchange measurem.nts. Results: Left and right ve ntricular TDi velocities were greater in controls than in patients. Left ventric ular ejection fraction (LVEF) correlated with Sm(r=0.30, p=0.0005), but not with em, Am, or the em/Am ratio. There were no significant differences between new Y ork Heart Association (nYHA) functional class for any of the TDi variables. Righ t ventricular indices were not related to exercise capacity. Systolic myocardial motion measured by TDi correlated more closely with peak oxygen consumption (p VO2) (r=0.35, pn LVEF (r=0.21,em/Am ratio was not corr elated with P VO2. In multiple regression, Sm was the only left ventricular TDi variable to predict exercise capacity independently(pnclusions: Exerc ise capacity and symptoms are poorly related to conventional measures of cardiac function and more closely correlated with indices of longitudinal left ventricu lar function as assessed by TDi.

关键词：慢性心力衰竭运动耐量心功能组织多普勒成像平板运动试验射血分数左室收缩功能收缩期峰值性别匹配左心室

神经功能恢复良好的动脉瘤性蛛网膜下腔出血患者18个月后的心理状况调查

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世界核心医学期刊文摘（神经病学分册） 2005年第1期1卷 17-18页

作者： Powell J. Kitchen n. Heslin J. Greenwood R. 李锐 Goldsm iths College Lewisham WayNewCrossLondonSE146N W United K ing-dom Dr.

Objectives: To evaluate functioning, 18 m onths after surgery,of 49patients with good neurological recovery following aneurysm alsubarachnoid haem orrhage (SAH ), and to determ ine the extent of any im provem ents in dis- turbances of m ood,cognitive functioning,and levels of activity and participation previously observed at 9m onth follow up.M ethods: SAH patients, m atched for age, gender,and occupation with healthy control participants, com pletely quantitative m easures ofm ood (H A D S,FIES, BD I)and activity /participation (BICR O -39scales),and a briefcognitive assessmentbattery (verbalfluency,digit span,prose recall).Controls com pleted theH A D S and the BICRO -***:Patients showed som e recovery of cognitive functioning,though im pairm ents of prose recall persisted.A nxiety and depression sym ptom s were higher in patients than in controls,butfewerthan 20% scored in the clinical range on any questionnaires except for RIES-Intrusive thoughts (22%);only three showed signs of full blown post-traum atic stress *** ost half showed elevated dependence on others for dom estic activi- ties and organisation and abnorm ally low levels ofem ploy- *** little variance in outcom e was predicted by dem ographic variables,neurological or cognitive im pair- m ent,prior life stress,orm ood.H owever,levels ofsocial activity and self-organisation were related to persisting fa- tigue. Conclusions: The observed decline in intrusive thoughts and avoidance over tim e is consistent with that seen after life threatening illness or traum *** persistent reductions in independence and levels of em ploym ent m ay in som e casesreflectconsidered lifestyle adjustm ents rather than adverse and unwanted changes butin others indicate a need forfocused rehabilitation.

关键词：心理状况调查功能恢复认知功能康复治疗情绪障碍认知损害记忆障碍流畅性职业与健康应激状态

Demographic Expansion and Contraction in a neotropical Fish during the Late Pleistocene-Holocene

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Open Journal of Statistics 2019年第4期9卷 470-483页

作者： Carolina L. n. Costa S. Ivan Perez José Louvise Carlos H. Tonhatti Rute B. G. Clem.nte-Carvalho Ana C. Petry S. F. dos Reis Programa de Pós-Graduacao em Ecologia Universidade Estadual de Campinas Sao Paulo Brazil División Antropología Facultad de Ciencias Naturales y Museo Universidad Nacional de La Plata CONICET La Plata Argentina Faculdade do Vale do Jaguaribe Ceará Brazil Programa de Pós-Graduacao em Genética Universidade Estadual de Campinas Sao Paulo Brazil Deparment of Biology Queen’s University Ontario Canada núcleo em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil Departamento de Biologia Animal Universidade Estadual de Campinas Sao Paulo Brazil

Demographic changes during the late Pleistocene-Holocene left signatures in the DnA of contemporary populations. These signatures reveal demographic phenomena like the increase or decrease in effective population size. In this paper we searched for signatures of demographic change in the DnA of the neotropical freshwater fish Poecilia vivipara. Also, we investigated whether demographic changes are correlated with palaeoclimatic events of the late Pleistocene-Holocene, in particular, if changes in effective population size are correlated with expansion and contraction of available habitats, induced by global ice-volume changes and sea-level fluctuations. We used Bayesian Skyline Plot (BSP) analysis with sequences from the mitochondrial gene cytochrome b to estimate the ancestral demography of the neotropical freshwater fish P. vivipara. To test the assumptions of neutrality and absence of population structure we used Tajima’s D and Spatial Analysis of Molecular Variance (SAMOVA), respectively. Effective population size of P. vivipara remained stable until 75,000 years ago, increased by 10-fold reaching a maximum at approximately 25,000 years ago, then suddenly declined at the Pleistocene-Holocene boundary. Variation in effective population size in P. vivipara correlates with expansion and contraction of habitats induced by sea-level fluctuations, caused by the advance and retreat of ice sheets during the Last Glacial Maximum (LGM).

关键词： Ancestral Population Dynamics Coalescence Bayesian Skyline Plot Mitochondrial DnA Last Glacial Maximum

Antibacterial Effect of Aqueous Extracts and Bioactive Chemical Compounds of <i>Coffea canephora</i>against Microorganisms Involved in Dental Caries and Periodontal Disease

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Advances in Microbiology 2014年第14期4卷 978-985页

作者： Flávia M. da Silva natalia L. P. Iorio Leandro A. Lobo Kátia Regina n. dos Santos Adriana Farah Lucianne C. Maia Andréa G. Antonio Departamento de Pós-Graduacao Mestrado Profissional em Odontologia Faculdade de Odontologia Universidade Veiga de Almeida Rio de Janeiro Brasil Departamento de Ciência Básica Universidade Federal Fluminense Nova Friburgo Brasil Departamento de Microbiologia Médica Instituto de Microbiologia Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Instituto de nutricao Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Departamento de Odontopediatria e Ortodontia Faculdade de Odontologia Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil

This study aimed to evaluate the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Coffea canephora aqueous extract (Cc), trigonelline (Tg) and 5-caffeoylquinic acid (5-CQA) against cariogenic microorganisms: Streptococcus parasanguinis ATCC 903 (SP), Lactobacillus rhamnosus ATCC 9595 (LR);and pathogens related to periodontal disease: Porphyromonas gingivalis ATCC 33277 (PG), Fusobacterium nucleatum ATCC 25586 (Fn), Prevotella intermedia ATCC 49046 (PI) and Prevotella nigrescens ATCC 33563 (Pn). Different concentrations of Cc (0.15625 to 10 mg/mL), Tg and 5-CQA (0.005 to 10.24 mg/mL) were tested. Chlorhexidine (0.05%) was used as positive control and the substances without the inoculum comprised the blank control. The Cc showed bacteriostatic action against SP (MIC = 5 mg/mL) and LR (MIC = 10 mg/mL). Tg showed bacteriostatic action against SP (MIC = 2.56 to 1.28 mg/mL), LR (MIC = 2.56 mg/mL), PG (MIC = 2.56 to 1.28 mg/mL), Fn (MIC = 5.12 mg/mL), Pn (MIC = 2.56 mg/mL), and PI (MIC = 2.56 to 1.28 mg/mL). Also, Tg showed bactericide properties against SP (MBC = 2.56 mg/mL), PG (MBC = 2.56 mg/mL), Fn (MBC = 10.24 mg/mL), Pn (MBC = 5.12 mg/mL), and PI (MBC = 2.56 mg/mL). Although 5-CQA has previously shown activity against Streptococcus mutans, in the present study, it showed no activity against all tested microorganisms. C. canephora extract only showed antibacterial activity against cariogenic microorganisms, not presenting action against periodontal pathogens. It was concluded that trigonelline presented the best effect against all pathogens tested, therefore coffee extracts with higher trigonelline content should be tested against these specific pathogens.

关键词： Oral Pathogens Dental Caries Periodontal Disease Coffee Trigonelline Chorogenic Acid

hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis

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World Journal of Gastroenterology 2016年第6期22卷 2060-2070页

作者： Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos Pós-Graduação em Genética e Biologia Molecular Universidade Federal do ParáCidade Universitária Prof.JoséSilveira NettoBelémPA 66075-970Brasil Laboratório de Genética Humana e Médica Instituto de Ciências BiológicasUniversidade Federal do ParáBelémPA 66075-970Brasil Pós-Graduação em.neurociências e Biologia Molecular Universidade Federal do ParáBelémPA 66075-970Brasil Laboratório de Citogenética Humana-Instituto de Ciências Biológicas Universidade Federal do ParáBelémPA 66075-970Brasil Hospital São Camilo e São Luís-Rua Dr.Marcelo Cândia MacapáAP 68901-901Brasil Hospital Universitário João de Barros Barreto Universidade Federal do ParáBelémPA 66075-970Brasil Instituto de Ciências da Saúde Universidade Federal do ParáBelémPA 66075-970Brasil

AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by AnOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric ***: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RnA(mi RnA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMnT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal m

关键词： Gastric cancer Gastric lesions Micro RnA Biomarker Carcinogenesis

用铁(Ⅱ)-1,10-邻菲啰啉-大黄素体系的共振瑞利散射光谱法测定生物样品中大黄素

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药物分析杂志 2015年第6期35卷 1010-1015页

作者：王二女陈科平杨季冬西南大学化学化工学院重庆400715 重庆市涪陵环境监测中心重庆408000 三峡学院化学与环境科学学院重庆404000

目的：建立共振瑞利散射法测定大黄素的含量。方法：在p H 7.3的B-R缓冲溶液中,铁（Ⅱ）与1,10-邻菲啰啉形成稳定的1∶3的配合物,再与大黄素（emO）结合形成1∶2的离子缔合物。铁（Ⅱ）-1,10-邻菲啰啉溶液：称取0.278 0 g Fe SO4·7H2O... 详细信息

目的：建立共振瑞利散射法测定大黄素的含量。方法：在p H 7.3的B-R缓冲溶液中,铁（Ⅱ）与1,10-邻菲啰啉形成稳定的1∶3的配合物,再与大黄素（emO）结合形成1∶2的离子缔合物。铁（Ⅱ）-1,10-邻菲啰啉溶液：称取0.278 0 g Fe SO4·7H2O溶于70 m L水中,加入邻菲啰啉（1,10-phen）0.595 0 g,加入适量的抗坏血酸,稀释至100 m L,得到1×10^-2mol·L^-1的储备液,用时稀释成2.0×10^-3mol·L^-1的工作液。室温下,于10 m L比色管中依次加入p H 7.3的B-R缓冲溶液2 m L,适量的emO标准溶液,以及2.0×10^-3mol·L^-1Fe（phen）2＋3溶液1.2 m L。每加一种试剂后混合均匀,用二次蒸馏水定容至刻度,摇匀,静置10 min后,在荧光分光光度计上以λex=λem方式进行同步扫描,记录共振瑞利散射（RRS）光谱,以λem=2λex和λem=1/2λex进行扫描,分别测量不同入射波长（λex）下的散射强度ISOS和IFDS,然后分别以ISOS和IFDS对应的波长作图,得到二级散射（SOS）光谱和倍频散射（FDS）光谱。分别在各自的最大散射波长处测量样品和试剂空白的散射强度IRRS,ISOS,IFDS及I0RRS,I0SOS,I0FDS,ΔI=I-I0。结果：体系的RRS、SOS和FDS显著增强并出现新的散射峰,相应的最大散射峰分别位于349、684和351 nm。emO的质量浓度在0.8-10.4μg·m L-1时,与RRS、SOS和FDS的散射强度呈良好的线性关系,其检出限（3σ）依次分别为10.1、32.8、28.6 ng·m L^-1。血样和尿样（各3批）中测定emO的回收率分别在94.9%-102.4%和99.4%-102.7%之间,RSD分别在1.5%-3.1%和1.1%-3.0%之间。将本法与紫外可见分光光度法比较,结果满意。结论：经方法学验证,体系的共振瑞利散射方法可用于尿样和血清中大黄素的测定。

关键词：大黄素铁(Ⅱ)-1,10-邻菲啰啉离子缔合物共振瑞利散射(RRS) 二级散射(SOS) 倍频散射(FDS) 生物样品测定

Persistence of Anti-SARS-CoV-2 IgM Antibody up to 8 Months Post-COVID-19

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Case Reports in Clinical Medicine 2021年第9期10卷 227-231页

作者： Carlos David A. Bichara Ednelza S. G. Amoras Gergiane L. Vaz Cléa n. C. Bichara Isabella P. C. Amaral Amaral Antonio C. R. Vallinoto Laboratório de Virologia Instituto de Ciências Biológicas Universidade Federal do Pará (UFPA) Belém Brasil Amaral Costa Medicina Diagnóstica Belém Brasil Faculdade de Medicina Centro de Ciências Biológicas e da Saúde Universidade do Estado do Pará (UEPA) Belém Brasil Programa de Pós-Graduaç ã o em Biologia de Agentes Infecciosos e Parasitários Instituto de Ciências Biológicas Universidade Federal do Pará (UFPA) Belém Brasil

Here, we present a longitudinal analysis of two patients who recovered from COVID-19 more than 8 months prior but showed persistent serological detection of anti-SARS-CoV-2 IgM. We still do not know the exact reason for this prolonged persistence of IgM in these patients. To our knowledge, these are the first reports of IgM persistence in the context of SARS-CoV-2 infection and point to the need for no longer using IgM as a diagnostic criterion for acute or recent COVID-19. One should opt for gold standard molecular methodologies due to their high sensitivity and specificity.

关键词： COVID-19 SARS-CoV-2 Antibodies IgM Persistence