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Seasonal variation and host sex affect bat–bat fly interaction networks in the Amazonian savannahs

Integrative Zoology 2024年第3期19卷 400-416页

作者： Paulo MEJIA Gustavo Lima URBIETA Bruna da Silva XAVIER IsaíJorge de CASTRO JoséJúlio DE TOLEDO Gustavo GRACIOLLI Lucio AndréVIANA DIAS William Douglas CARVALHO Programa de Pós-Graduação em Biodiversidade Tropical Universidade Federal do Amapá(UNIFAP)MacapáAPBrazil Programa de Pós-Graduação em Ecologia Instituto de BiologiaCentro de Ciências da SaúdeUniversidade Federal do Rio de Janeiro(UFRJ)Rio de JaneiroRJBrazil Laboratório de Mamíferos Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá(IEPA)MacapáAPBrazil Programa de Pós-Graduação em Biologia Animal Laboratório de SistemáticaEcologia e Evolução(LSEE)Instituto de BiociênciasUniversidade Federal de Mato Grosso do Sul(UFMS)Campo GrandeMTBrazil Terrestrial Ecology Group(TEG-UAM) Departamento de EcologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Centro de Investigación en Biodiversidad y Cambio Global(CIBC-UAM) Universidad Autónoma de MadridMadridSpain

Bats are the second-most diverse group of mammals in the world,and bat flies are their main ***,significant knowledge gaps remain regarding these antagonistic interactions,especially since diverse factors such as seasonality and host sex can affect their network ***,we explore the influence of such factors by comparing species richness and composition of bat flies on host bats,as well as specialization and modularity of bat–bat fly interaction networks between seasons and adult host *** captured bats and collected their ectoparasitic flies at 10 sampling sites in the savannahs of AmapáState,northeastern region of the Brazilian *** female bats being more parasitized and recording greater bat fly species richness in the wet season,neither relationship was statistically *** pooled network could be divided into 15 compartments with 54 links,and all subnetworks comprised>12 *** total number of links ranged from 27 to 48(for the dry and wet seasons,respectively),and female and male subnetworks had 44 and 41 links,*** values were very low for the pooled network and for all *** results revealed higher bat fly species richness and abundance in the wet season,whereas specialization and modularity were higher in the dry ***,the subnetwork for female bats displayed higher specialization and modularity than the male ***,both seasonality and host sex contribute in different ways to bat–bat fly network *** studies should consider these factors when evaluating bat–bat fly interaction networks.

关键词： Amapá Amazon biome bat flies bats interaction networks

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Circulating extracellular vesicles:friends and foes in neurodegeneration

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Neural Regeneration Research 2022年第3期17卷 534-542页

作者： Anna Picca Flora Guerra Riccardo Calvani Hélio JoséCoelho-Junior Cecilia Bucci emanuele Marzetti Fondazione Policlinico Universitario“Agostino Gemelli”IRCCS RomeItaly Aging Research Center Department of NeurobiologyCare Sciences and SocietyKarolinska Institutet and Stockholm UniversityStockholmSweden Department of Biological and Environmental Sciences and Technologies Universitàdel SalentoLecceItaly UniversitàCattolica del Sacro Cuore Institute of Internal Medicine and GeriatricsRomeItaly

Extracellular vesicles have been identified as pivotal mediators of intercellular communication with critical roles in physiological and pathological *** this route,several molecules(e.g.,nucleic acids,proteins,metabolites) can be transferred to proximal and distant targets to convey specific *** vesicle-associated cargo molecules have been proposed as markers of several disease conditions for their potential of tracking down the generating ***,circulating extracellular vesicles may represent biomarkers of dysfunctional cellular quality control systems especially in conditions characterized by the accrual of intracellular misfolded ***,the identification of extracellular vesicles as tools for the delivery of nucleic acids or other cargo molecules to diseased tissues makes these circulating shuttles possible targets for therapeutic *** increasing interest in the study of extracellular vesicles as biomarkers resides mainly in the fact that the identification of peripheral levels of extracellular vesicle-associated proteins might reflect molecular events occurring in hardly accessible tissues,such as the brain,thereby serving as a "brain liquid biopsy".The exploitation of extracellular vesicles for diagnostic and therapeutic purposed might offer unprecedented opportunities to develop personalized ***,we discuss the bright and dark sides of extracellular vesicles in the setting of two main neurodegenerative diseases(i.e.,Parkinson's and Alzheimer's diseases).A special focus will be placed on the possibility of using extracellular vesicles as biomarkers for the two conditions to enable disease tracking and treatment monitoring.

关键词： Alzheimer's disease amyloid protein exosomes misfolded proteins mitochondrial-derived vesicles neuroinflammation Parkinson's disease quality control Tau protein α-synuclein

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Simulating Ocean and Tidal Current Power Plants with Homer

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Computational Water, Energy, and Environmental Engineering 2015年第3期4卷 38-55页

作者： Andrea Fischer Jones S. Silva Alexandre Beluco Luiz em.lio B. Almeida Instituto Federal Sul Riograndense (IFSUL) Pelotas RS Brazil Instituto de Pesquisas Hidráulicas Universidade Federal do Rio Grande do Sul (UFRGS) Porto Alegre Brazil

The oceans can provide us with several direct sources of renewable energy, such as wave energy, energy from currents and energy in thermal gradients among other energy resources. The uses of these resources have not yet reached technical and economic maturity, but they have experienced significant advances in recent years. In this process, tools for feasibility analysis of hybrid systems including specific power plants are important. This article is dedicated to the feasibility study of hybrid systems containing power plants based on the energy of ocean and tidal currents. The software currently available for feasibility studies do not present specific models already developed for the simulation of this kind of power plants. Thus, this technical note shows how Homer software (Legacy version) can be used to conduct this type of study in pre-feasibility level. This article also presents a case study in which Homer is applied, focused only on Homer adaptation but without exhausting the subject, showing a feasibility window that would include current power plants with certain cost and efficiency in an existing PV wind biodiesel hybrid system.

关键词： Ocean Energy Ocean Current Energy Tidal Current Energy Computational Simulation Feasibility Study Software Homer

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Association between Infrared Thermography, Blood Count and Creatine Kinase in the Evaluation of the Welfare of Vaquejada Horses

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Open Journal of Veterinary Medicine 2023年第6期13卷 53-67页

作者： Carolina Jones Ferreira Lima da Silva Keity Laiane Gomes Trindade Raissa Karolliny Salgueiro Cruz César Fabiano Vilela Clarisse Simões Coelho José Dantas Ribeiro Filho Helena emília Cavalcanti da Costa Cordeiro Manso Hélio Cordeiro Manso Filho Núcleo de Pesquisa Equina Universidade Federal Rural de Pernambuco Recife Brazil Faculdade de Medicina Veterinária Centro Universitário Cesmac Maceió Brazil Médico Veterinário Privado Americana Brazil Faculdade de Medicina Veterinária Universidade Lusófona de Humanidades e Tecnologias Lisboa Portugal Departamento de Medicina Veterinária Universidade Federal de Vi&#231osa Vi&#231osa Brazil

The Quarter-Horses regularly participate in non-traditional sports that simulate activities on cattle ranches. In the vaquejada races, the horses run at about 30 km/h, reaching maximum heart rates of around 200 bpm, indicating the great physical effort during competitions and with that could impact welfare. To test the hypothesis that vaquejada horses can be evaluated for the quality of welfare through a combination of non-invasive and invasive methods, an experiment was developed that aimed to assess the quality of welfare through thermography and blood biomarkers before and after vaquejada races. Ten vaquejada horses, which were in regular competition, were submitted to the vaquejadas racing test were used. Thermography was performed in 14 regions of interest (head, neck and thorax) in the following phases: pre-race and +1, +4 and +24 hours of the races. Blood samples were collected in pre-race, immediately after and +1, +4 and +24 hours of the races. Results were submitted to ANOVA and Tukey’s test, with p set at 5%. Thermography showed that local temperatures before the race and +24 hours after the races were similar (p > 0.05), as well as between +1 and +4 hours after the races (p > 0.05), which showed higher surface temperatures than the first group (pre-race and +24 hours) (p 0.05) after the exercises. In conclusion, the vaquejada horses evaluated by infrared thermography and blood analyses maintained the quality of well-being, with the physiological elevation of several parameters after the races and recovery in less than 24 hours after the races. Thermographic images were also able to help in the analysis of the regions of interest that have contact with harnesses, demonstrating that the use of adequate and correct equipment does not compromise animal welfare.

关键词： Exercise Equine CK Hematology Equestrian Sports Well-Being

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The Effects of Different Titanium Surfaces on the Behaviour of Osteoblast-Like Cells

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Journal of Biomedical Science and Engineering 2015年第6期8卷 380-388页

作者： Fabiano Ribeiro Cirano Adriane Yaeko Togashi Marcia Martins Marques Francisco em.lio Pustiglioni Luiz Antonio Pugliesi Alves Lima Department of Periodontology Paulista University(UNIP)Sao PauloBrazil Department of Dental Implantology State University of Western Parana(UNIOESTE)CascavelBrazil Department of Dentistry University of Sao PauloSao PauloBrazil Department of Periodontology University of Sao PauloSao PauloBrazil

This study investigated the influence of different titanium surfaces on the differentiation of rat osteoblast-like cells (osteo-1). Osteo-1 cells were cultured on the following titanium surfaces: 1) pretreated, smooth surface (PT);2) sandblasted and acid etched surface (SLA);and 3) sandblasted and acid-etched surface rinsed under nitrogen protection to prevent exposure to air and preserved in isotonic saline solution (modSLA). Cell metabolism, total protein content, collagen content and alkaline phosphatase (AP) activity and the formation of calcified nodules were analyzed. The titanium surface did not influence cell metabolism, total protein content and collagen content. The SLA surface influenced cell differentiation, with the observation of a significant reduction of AP activity and formation of calcified nodules after 21 days compared to the PT surface. No difference was observed between the PT and modSLA surfaces. All titanium surfaces tested permitted the full expression of the osteoblast phenotype by osteo-1 cells, including matrix mineralization.

关键词： Titanium Dental Implants Osteoblasts

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Use of Caffeine for the Evaluation of the Anthropic Influence over the Upper and Middle Iguaçu River Basins

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Journal of Water Resource and Protection 2022年第3期14卷 273-291页

作者： Bruna Scipioni Márcia Cristiane Kravetz Andrade Luis Otávio Miranda Peixoto Alinne Mizukawa Tais Cristina Filippe Júlio César Rodrigues De Azevedo Programa de Pós-Graduaç ã o em Engenharia de Recursos Hídricos e Ambiental Universidade Federal do Paraná Curitiba Brasil Universidade Tecnológica Federal do Paraná Curitiba Brasil

Many parameters are usually used as gauges to describe water quality worldwide, such as the concentrations of phosphorus, forms of nitrogen, dissolved oxygen (DO), among others. Though legislative limits for the concentrations of these substances are in place, such is not the case for caffeine. Caffeine is a pharmacologically active alkaloid, from the xanthine group, which can be used as a chemical tracer for anthropic influence over water resources. The objective of this study was to analyze the pollution indicators over the Igua?u River, one of the main rivers in Southern Brazil, through the examination of nutrients, DO and their correlations to caffeine. The water samples were collected from 10 sampling sites along the river course, unevenly spaced to better represent the different environmental scenarios present in its basin, extracted from 2014 to 2017. Caffeine was detected and quantified through high-efficiency liquid state chromatography. Results show that both nutrient and caffeine concentrations increased in intensely urbanized areas, specially from IG2 to IG5 sampling sites. Downstream from IG6 the contaminant concentrations decreased mainly due to higher river flows and lower population density. Caffeine was detected in 82% of the samples, indicating that most of the sampling sites were under anthropic influence.

关键词： Water Resources Water Quality Caffeine Urban Areas

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ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

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Journal of Pharmacy and Pharmacology 2019年第7期7卷 368-384页

作者： Kamilla Monteiro dos Santos Izabela Natalia Faria Gomes Renato JoséSilva Oliveira Fernanda E.Pinto Bruno Oliveira Fabrício P.Batista Rafael Cesar russo Chagas Hélio B.Santos Ralph G.Thomé Wanderson Romao Rui Manuel Reis Maria Luiza Vilela Oliva Rosy Iara Maciel de Azambuja Ribeiro Laboratory of Experimental Pathology Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Josédo Rosário Vellano University UnifenasDivinópolisMGBrazil Molecular Oncology Research Center(CPOM) Barretos Cancer HospitalBarretosSPBrazil Petroleomic and Forensic Chemistry Laboratory Department of ChemistryFederal Institute of Espírito Santo(IFES)VitóriaESBrazil Chemistry Department Federal University of Sao PauloBrazil Tissue Processing Laboratory Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Life and Health Sciences Research Institute(ICVS) Medical SchoolUniversity of MinhoBragaPortugal 3ICVS/3B’s-PT Government Associate Laboratory GuimaraesBragaPortugal

Background:The breast cancer has been the most common form of cancer among *** triple negative subtype represents 20%of all breast cancer cases in the world and is standing out by affecting young women and being *** main cause of death of patients with cancer is due to metastasis,which can reach the liver and ***:The activities of ID7 fraction of the stems of Bauhinia variegata *** breast cancer,lung metastasis and liver inflammatory process were ***:ID7 was characterized by *** viability of murine mammary cells(4T1)treated with ID7 was assessed by MTT,trypan blue and fluorescence assay and viability of BT-20,MDA-MB-231 and MCF-7 human breast cancer tumor lines by *** cell migration,invasion using matrigel and adhesion were *** expression of cell death proteins was quanitified by western blot and the gelatinases by *** ID7 activity of the tumor(4T1)and metastatic progession in vivo was ***:ID7 reduced the 4T1 and MDA-MB-231viability and increased the late apoptosis,inhibited the 4T1 migration and invasion,increased the 4T1 adhesion and decreased the secreted active ***7 also increased the expression of PARP,caspase-7 and caspase-8,RIP and *** vivo,the ID7 decreased the volume and weight of the tumors and decreased lung metastasis and inflammation in the *** characterization showed mainly the presence of oleic acid,myricetin,quercetin and kaempferol in ***:Thus,it was found that ID7 fraction exhibits selective antitumor and on the mechanisms of breast cancer metastasis activity,preventing lung metastasis and inflammation in the *** is suggested that fatty acids and flavonoids are correlated with such activities.

关键词： 4T1 cells MDA-MB-231 medicinal plants kaempferol gelatinases apoptosis cell viabilty in vivo

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Development and Body Composition of Quarter Horse Foals during Nursing

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Open Journal of Veterinary Medicine 2014年第11期4卷 276-280页

作者： Monica Miranda Hunka Helena emília Cavalcanti da Costa Cordeiro Manso Ramona Bastos Bernardo Elizabeth Regina Rodrigues da Silva Lúcia Maia Cavalcante Ferreira Hélio Cordeiro Manso Filho Núcleo de Pesquisa Equina Departamento de Zootecnia Universidade Federal Rural de Pernambuco Recife Brazil

The monitoring and development of foals, based on biometric indices, and the determination of body composition could be important for genetic improvement and the elaboration of nutritional plans for horses. Considering that little information was available, the aim of the present study was to assess the growth and determine the body composition of suckling Quarter Horse foals. Animals (n = 46) of both sexes were monitored monthly from birth until the fifth month of age, using an ultrasound device and the following measurements: Wither Height (WH);Chest Perimeter (CP);Cannon Bone Circumference (CBC) Cannon Bone Girth (CG);Body Mass (BM);and Rump Fat Thickness (RFT). These tests determined the Fat-Free Mass (FFM), BM, Fat Percentage (FP) and Fat Thickness (FT). The data were analyzed using ANOVA and Tukey’s test, with differences considered statistically significant when P ≤ 0.05. The results demonstrated that the parameters analyzed changed significantly during the study period. The BM was ~41 at birth and ~177 kg at the point of weaning. Conversely, the FM was ~3.8 at birth and 18.1 kg at the point of weaning. The FFM was ~37 and ~160 kg at the time of birth and weaning, respectively. The FP varied from 9.3 at birth to 9.54 at weaning. The mean FT was ~1.39 and ~1.91 cm at birth and weaning, respectively. The WH measured ~91 at birth and 116 cm at weaning. In conclusion, suckling foals gain BM quickly from the time of birth to the time of weaning. The main cause of this gain is FFM. Therefore, due to the significant changes in the parameters studied herein, it is important to monitor these values in order to prevent nutritional imbalances.

关键词： Body Mass Fat-Free Mass Fat Percentage Horse Ultrasound

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Parameter Estimations for Generalized RayleighDistribution under Progressively Type-I IntervalCensored Data

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Open Journal of Statistics 2011年第2期1卷 46-57页

作者： Y. L. lio Ding-Geng Chen Tzong-Ru Tsai 不详

In this paper, inference on parameter estimation of the generalized Rayleigh distribution are investigated for progressively type-I interval censored samples. The estimators of distribution parameters via maximum likelihood, moment method and probability plot are derived, and their performance are compared based on simulation results in terms of the mean squared error and bias. A case application of plasma cell myeloma data is used for illustrating the proposed estimation methods.

关键词： Maximum Likelihood Estimate Method of Moments em Algorithm Type-I Interval Censoring

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Performance of a serological IgM and IgG qualitative test for COVID-19 diagnosis:An experimental study in Brazil

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World Journal of Experimental Medicine 2022年第5期12卷 100-103页

作者： Fabrício Freire de Melo Lilian Martins Oliveira Diniz JoséNélio Januário João Fernando Gonçalves Ferreira Rafael Santos Dantas Miranda Dórea Breno Bittencourt de Brito Hanna Santos Marques Fabian Fellipe Bueno Lemos Marcel Silva Luz Samuel Luca Rocha Pinheiro Dulciene Maria de Magalhães Queiroz Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Departamento de Pediatria Universidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Núcleo de Ações e Pesquisa em Apoio Diagnóstico Universidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Laboratory of Research in Bacteriology Universidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Laboratory of Bacteriology Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45029-094Brazil

Qualitative antibody tests are an easy,point-of-care diagnostic method that is useful in diagnosing coronavirus disease 2019,especially in situations where reverse transcription-polymerase chain reaction is ***,some factors are able to affect its sensitivity and accuracy,which may contribute to these tests not being used as a first-line diagnostic tool.

关键词： Serological test IgM IgG COVID-19 Diagnosis Antibody

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