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Chaperoning glucocerebrosidase:a therapeutic strategy for both Gaucher disease and Parkinsonism

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Neural Regeneration Research 2016年第11期11卷 1760-1761页

作者： Benjamin McMahon elma Aflaki Ellen Sidransky Medical Genetics Branch National Human Genome Research InstituteNational Institutes of Health

Gaucher disease （GD） is a lysosomal storage disorder （LSD） affecting approximately 1 in 50,000 individuals in the general population. Mutations in both alleles of the GBA1 gene result in deficient glucocerebrosidase （GCase） activity, which in turn leads to the accumulation of glycolipid substrates and impaired lysosomal function. GD is a multisystern disorder with a vast spectrum of clinical phenotvpes,

关键词： GBA Chaperoning glucocerebrosidase GD

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Vitamin D therapy in pediatric patients with inflammatory bowel disease:a systematic review and meta-analysis

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World Journal of Pediatrics 2023年第1期19卷 48-57页

作者： Mohammad Hassan Sohouli Fatemeh Farahmand Hosein Alimadadi Parisa Rahmani Farzaneh Motamed elma Izze da Silva Magalhaes Pejman Rohani Pediatric Gastroenterology and Hepatology Research Center Pediatrics Centre of ExcellenceChildren's Medical CenterTehran University of Medical SciencesTehranIran Department of Clinical Nutrition and Dietetics Faculty of Nutrition and Food TechnologyShahid Beheshti University of Medical SciencesTehranIran Postgraduate Programme in Collective Health Federal University of MaranhaoRua Barao de Itapary155CentroSao LuísMABrazil Pediatrics Gastroenterology Department of PediatricsSchool of MedicineChildren's Medical CenterTehran University of Medical SciencesTehranIran

Background There is some evidence for the role of vitamin D deficiency in the pathogenesis of inflammatory bowel disease(IBD)in the pediatric ***,the results are ***,we have conducted a systematic review and meta-analysis to evaluated the effect of vitamin D on pediatric patients with *** We carried out a systematic search in databases from inception until 20 January *** included all relevant articles that evaluate the efficacy and safety of vitamin D on disease activity,inflammatory factors,and vitamin D and calcium levels in pediatric patients with *** effects models were used to combine the *** main outcomes were then analyzed using weight mean difference(WMD)and respective 95%confidence interval(CI).Results Fifteen treatment arms met the eligibility criteria and were *** estimates indicated that intervention with vitamin D has a significantly beneficial effect on 25-hydroxy vitamin D3[25(OH)D3](pooled WMD of 17.662 ng/mL;CI 9.77-25.46;P<0.001),calcium(pooled WMD of 0.17 mg/dL;CI 0.04-0.30;P=0.009),and inflammatory factors including C-reactive protein(CRP)(pooled WMD of-6.57 mg/L;CI-11.47 to-1.67;P=0.009)and erythrocyte sedimentation rate(ESR)(pooled WMD of-7.94 mm/h;CI-12.65 to-3.22;P=0.001)*** addition,this effect was greater for vitamin D levels at doses greater than 2000 IU,and when follow-up duration was more than 12 *** This study showed that vitamin D therapy can have a significant and beneficial effect on 25(OH)D3,calcium,and inflammatory factors in children and adolescents with IBD.

关键词： Disease activity index Inflammation Meta-analysis IBD Pediatric Vitamin D

Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls

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Frontiers of Environmental Science & Engineering 2021年第2期15卷 33-42页

作者： Karla IlićĐurđić Raluca Ostafe Olivera Prodanović AleksandraĐurđevićĐelmaš Nikolina Popović Rainer Fischer Stefan Schillberg Radivoje Prodanović University of Belgrade-Faculty of Chemistry Studentski trg 12-1611000 BelgradeSerbia Molecular Evolution Protein Engineering and Production facility(MEPEP) Purdue UniversityWest LafayetteIN 47907USA Institute of Molecular Biotechnology RWTH Aachen University52074 AachenGermany Institute for Multidisciplinary Research Kneza Višeslava 111030 BelgradeSerbia Departments of Biological Sciences and Chemistry Purdue UniversityWest LafayetteIN 47907USA Fraunhofer Institute for Molecular Biology and Applied Ecology IME 52074 AachenGermany

The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation *** peroxidase(LiP)from Phanerochaete chrysosporium is a hemecontaining lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules,including industrial *** enzyme is therefore ideal as a starting point for protein ***,we subjected two positions(165 and 264)in the environment of the catalytic Trp171 residue to saturation mutagenesis,and the resulting library of 104 independent clones was expressed on the surface of yeast *** yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more *** identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes(Evans blue,amido black 10B and Guinea green)and up to 13-fold higher catalytic ***,cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis,achieving significant increases in both enzyme activity and stability compared to a whole-cell *** cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 *** best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.

关键词： Bioremediation Enzyme immobilization Protein engineering Yeast surface display

Glycosidically Bound Volatile Compounds of Satureja montana L.,*** Ten.,*** *** Endemic *** SiliC

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Advances in Biological Chemistry 2015年第7期5卷 235-238页

作者： Valerija Dunkic Ivana Radovanovic Nada Bezic elma Vuko Department of Biology Faculty of ScienceUniversity of SplitSplitCroatia

In this paper, the glycoconjugated volatile compounds of four Croatian Satureja species (Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and endemic S. visianii Silic) were investigated. Content and composition of these compounds were examined depending on the stage of plant development. GC and GC–MS analysis of volatile aglycones revealed twenty-one compounds. Thymoquinone, geraniol and carvacrol were detected in all vegetative phases of the investigated plants. Other quantitatively important aglycones were eugenol and thymol of S. montana, phenyl ethyl alcohol, benzene acetaldehyde, borneol, α-terpineol, thymol and eugenol of S. cuneifolia, phenyl ethyl alcohol, benzene acet-aldehyde, terpinen-4-ol, α-terpineol and β-ionone of S. subspicata and camphor, thymol and 8a-acetoxylemolol of S. visianii. Moderate similarity in the chemical composition of essential oils and volatile aglycones of investigated plant species indicate that many biologically active compounds are glycosylated and accumulate as non-volatile glycosides.

关键词： Satureja montana S.cuneifolia S.subspicata S.visianii Glicosydically Bound Volatiles Free Aglycones

像一条标准线——信号传输的串行结构使背连线板成为比赛跑道

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现代制造 2004年第2期 40-41页

作者： elma Ternew

“广为流传的串行传输技术和基础设施虽说不可能一夜之间被突然取代,但是背连线板今后仅仅面向串行传输”,国际标准化机构成员以及总线结构和工业网络顾问Hermann Strass先生曾断言。PICMG(CompactPCI)和VITA(VME总线)为由它们标准化了... 详细信息

“广为流传的串行传输技术和基础设施虽说不可能一夜之间被突然取代,但是背连线板今后仅仅面向串行传输”,国际标准化机构成员以及总线结构和工业网络顾问Hermann Strass先生曾断言。PICMG(CompactPCI)和VITA(VME总线)为由它们标准化了的传输技术准备了解决方案,或者说将它们标准化了。在GHz范围内的处理速度以及高速数据传输速率,要求从机械方面到热管理一直到供电方面做系统的改动。

关键词： VME总线数据传输速率数据总线标准化信号传输串行结构

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Synthesis, characterization, and stability of iron (III) complex ions possessing phenanthroline-based ligands

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Open Journal of Inorganic Chemistry 2013年第1期3卷 7-13页

作者： Shawnt Tosonian Charles J. Ruiz Andrew Rios elma Frias Jack F. Eichler Department of Chemistry University of California Riverside Riverside USA

It has previously been demonstrated that phenanthroline-based ligands used to make gold metallotherapuetics have the ability to exhibit cytotoxicity when not coordinated to the metal center. In an effort to help assess the mechanism by which these ligands may cause tumor cell death, iron binding and removal experiments have been considered. The close linkage between cell proliferation and intracellular iron concentrations suggest that iron deprivation strategies may be a mechanism involved in inhibiting tumor cell growth. With the creation of iron (III) phen complexes, the iron binding abilities of three polypyridal ligands [1,10-phenanthroline (phen), 2,9-dimethyl-1, 10-phenanthroline (methylphen), and 2,9-di-sec-butyl-1, 10-phenanthroline (sec-butylphen)] can be tested via a competition reaction with a known iron chelator. Therefore, iron (III) complexes possessing all three ligands were synthesized. Initial mass spectrometric and infrared absorption data indicate that iron (III) tetrachloride complex ions with protonated phen ligands (RphenH+) were formed: [phenH][FeCl4], [methylphenH][FeCl4], [sec-butylphenH][FeCl4]. UV-vis spectroscopy was used to monitor the stability of the complex ions, and it was found that the sec-butylpheniron complex was more stable than the phen and methylphen analogues. This was based on the observation that free ligand was observed immediately upon the addition of EDTA to the [phenH][FeCl4] and [methylphenH] [FeCl4] complex ions.

关键词： Polypyridyl Ligands Phenanthroline Iron (III) Complex Ions

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胰腺乳头状囊性肿瘤

放射学实践 1997年第1期12卷 14-16页

作者： elma.,M 汪玲同济医科大学附属同济医院

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Fosfomycin—A Promising Oral Antibiotic for the Treatment of Urinary Tract Infection (UTI)

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Open Journal of Urology 2022年第5期12卷 257-270页

作者： Sadia Sharmin Sheikh Mohammed Mostofa Kamal Md. Alimur Reza Khandokar Mohammad Ashik Elahi Sheikh Mohammed Mostofa elma Bushra Habib Department of Microbiology Anwer Khan Modern Medical College Hospital Dhaka Bangladesh Department of Medical Affairs Beximco Pharmaceuticals Limited Dhaka Bangladesh Department of Pharmacology Anwer Khan Modern Medical College Hospital Dhaka Bangladesh

Background: Among the common morbid causes that are prevalent among all age groups, urinary tract infections top the list. In our country, most of the UTI patients visiting hospitals (OPD or IPD) are already on empirical antibiotics even before getting the sensitivity report in their hand. The purpose of this research was to examine the susceptibility patterns of commonly used antibiotics, particularly fosfomycin, against common uropathogens. Methodology: This was a prospective observational study conducted between January and March 2021 in four private tertiary hospitals in Dhaka, Bangladesh. All the urine samples referred to these four laboratories, obtained from patients of all ages and both genders, clinically diagnosed to have UTI, were included. Urine culture was performed by a semiquantitative method on blood agar media and MacConkey agar media. Following identification, antimicrobial sensitivity testing was performed using the modified Kirby Bauer disk diffusion method in accordance with CLSI standards. The data was put into Statistical Package for Social Sciences (SPSS) statistical software version 25 for the analysis. Results: A total of 5389 urine samples were received from four private hospitals in Dhaka over three months, and of these, 934 (17.33%) isolates were obtained from culture. About 95% of the isolates were gram-negative bacilli (GNBs). The most common isolate was Escherichia coli 615 (65.85%), followed by Klebsiella spp. 154 (16.49%), Pseudomonas spp. 64 (6.85%) and 51 (5.46%) isolates of Enterobacter. Among the gram-positive cocci, the most common were Enterococci fecalis 18 (1.93%) and Staphylococcus aureus 17 (1.82%). Of all the antibiotics tested, fosfomycin sensitivity was 98.4%, 88.88%, and 100% for Escherichia coli, Enterococci fecalis, and Staphylococcus aureus, respectively. All the isolates tested were susceptible to Meropenem (77% - 100%), Amoxiclav (78% - 100%) and Nitrofurantoin (45% - 94%). Sensitivity amongst all the uropathogens

关键词： Antibiotic Susceptibility Fosfomycin Urinary Tract Infections

Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls

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Frontiers of Environmental Science & Engineering 2021年第2期 14-23页

作者： Karla Ili? ?ur?i? Raluca Ostafe Olivera Prodanovi? Aleksandra ?ur?evi? ?elma? Nikolina Popovi? Rainer Fischer Stefan Schillberg Radivoje Prodanovi? University of Belgrade-Faculty of Chemistry Molecular Evolution Protein Engineering and Production facility (MEPEP) Purdue University Institute of Molecular Biotechnology RWTH Aachen University Institute for Multidisciplinary Research Departments of Biological Sciences and Chemistry Purdue University Fraunhofer Institute for Molecular Biology and Applied Ecology IME

The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation *** peroxidase(LiP) from Phanerochaete chrysosporium is a hemecontaining lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules,including industrial *** enzyme is therefore ideal as a starting point for protein ***,we subj ected two positions(165 and 264) in the environment of the catalytic Trp171 residue to saturation mutagenesis,and the resulting library of 10～4 independent clones was expressed on the surface of yeast *** yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more *** identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes(Evans blue,amido black 10 B and Guinea green) and up to 13-fold higher catalytic ***,cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis,achieving significant increases in both enzyme activity and stability compared to a whole-cell *** cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 *** best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.

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