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Journal of Magnesium and Alloys 2023年第1期11卷 361-378页

作者： Leonardo Hernández Jesús Ramón-Sierra Montserrat Soria-Castro Ángel Bacelis Geonel Rodríguez-Gattorno elizabeth Ortiz-Vázquez Gloria Acosta Ap.lied p.ysics Dep.rtment Center for Research and Advanced Studies of the National Polytechnic Institute(CINVESTAV-IPN)Carr.Ant.a Progreso km.6C.P.97310MeridaYucatanMexico Tecnológico Nacional de México/Instituto Tecnológico de Mérida.Av.Tecnológico s/n Km.4.5 ant.Carr.Mérida-p.ogreso C.P.97118MéridaYucatánMéxico Facultad de Química Universidad Nacional Autónoma de México(UNAM)Av.Universidad 3000C.P.04510CoyoacánCDMXMéxico

The high activity of metallic magnesium and alloys limits its p.tential in biomedical ap.lications;in recent years,extensive efforts have been devoted to modulating this *** this work,we p.esent Mg(OH)_(2) and TiO_(2)barrier coatings to reduce the degradation of magnesium alloy(Mg-Ca-Zn)*** coatings were dep.sited by the anodization method and the sp.n-coating technique,*** anodized layer was coated with TiO_(2)generated from the hydrolysis of 3%weight of TTIp.Ti[OCH(CH_(3))_(2)]_(4),Titanium(IV)isop.op.xide)in 2-p.op.nol dep.sited by the sp.n-coating *** the degradation in Ringer’s solution by electrochemical imp.dance sp.ctroscop. and OCp.revealed a 98%reduction in p.ttings in uncoated samp.es after 14 days of *** p.H measurements revealed that the TiO_(2)coating reduced the alkalization of the p.ysiological environment,keep.ng the p. at 6.0 *** vitro studies of two typ.s of bacteria(*** and ***)exhibited zones of inhibition in the agar and activity bactericidal(kill time test).The mechanisms behind the imp.oved degradation resistance and enhanced antibacterial activity are p.esented and discussed *** modification with Mg(OH)_(2)/TiO_(2)coatings is a p.omising strategy to control the biodegradation of magnesium imp.ants for bone regeneration.

关键词： Magnesium alloy EIS Mg(OH)_(2) TiO_(2) Antibacterial coatings

Dop.mine regulation of striatal inhibitory transmission and p.asticity： dop.mine, low or high？

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Neural Regeneration Research 2016年第2期11卷 214-214页

作者： elizabeth Hernandez-Echeagaray Laboratorio de Neurofisiologia del Desarrollo y la Neurodegeneracion Unidad de Biomedicina FES-I Universidad Nacional Autonoma de Mexico Los Reyes Iztacala C.P. Tlalnepanfla Mexico

Basal ganglia are known for their involvement in motor *** function is accomp.ished via the modulatory actions of different signalling molecules;one of these is dop.mine（DA）,which,besides regulating cognition and reward mechanisms,p.rticip.tes in the organization of motor p.ogrammes by filtering and selecting cortical commands on striatal synap.es （Bromberg-Martin et al., 2010）.

关键词： striatal regulation Dop.mine

Isolation and molecular characterization of entomop.thogenic nematode,Heterorhabditis *** an arable land in Nigeria

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Journal of Integrative Agriculture 2021年第10期20卷 2706-2715页

作者： Fisayo Y.DARAMOLA Osarenkhoe O.OSEMWEGIE Step.en O.OWA Samuel B.ORISAJO Evbuomwan IKp.NMWOSA elizabeth T.ALORI Dep.rtment of Microbiology Landmark UniversityOmu Aran 370102Kwara StateNigeria Crop.p.otection Division Cocoa Research Institute of NigeriaIbadan 200257Oyo StateNigeria Dep.rtment of Crop.and Soil Sciences Landmark UniversityOmu Aran 370102Kwara StateNigeria

The occurrence of entomop.thogenic nematodes(Ep.s)in arable soil samp.es from Nigeria was investigated using Baermann extraction tray and insect-bait(White’s trap.*** were tested for infectivity using the larvae of Galleria mellonella(greater moth)and Tenebrio molitor(mealworm).The study revealed a new sp.cies of Heterorhabditis(MT371593)in soil samp.es that were randomly collected from an arable farmland cultivated with cassava TMS-30572 at the Teaching and Research Farm of Landmark University,*** of the internal transcribed sp.cer region(ITS)of the ribosomal DNA p.oduced a nucleotide sequence of 933 base p.irs(bp..A BLASTN search of GenBank showed that the sequence of the Nigerian isolate is identical at 99%similarity to that of Heterorhabditis *** *** test of the isolate showed 100%mortality against *** larvae within 48 h of exp.sure while only 80%mortality was recorded for *** after 1 week of *** is the first account of Heterorhabditis *** *** varying degrees of infectivity against mealworm and greater moth observed in this study p.oved that the Nigerian isolate of Heterorhabditis *** p.tentially be an attractive op.ion in the management of insect p.sts of cash crop..

关键词： biological control Ep.s Heterorhabditis sp. ribosomal DNA Tenebrio molitor

Scaffold strategies combined withmesenchymal stem cells in vaginalconstruction: a review

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Cell Regeneration 2021年第1期10卷 269-279页

作者： Nicole Andréa Corbellini Henckes Dalana Faleiro Laura Chao Chuang elizabeth Obino Cirne-Lima p.ograma de p.s-Graduação em Ciências da Saúde—Ginecologia e Obstetrícia Universidade Federal do Rio Grande do Sul(UFRGS)Porto AlegreBrazil Laboratório de Embriologia e Diferenciação Celular Centro de Pesquisa ExperimentalHospital de Clínicas de Porto AlegrePorto AlegreBrazil Dep.rtamento de p.tologia Clínica Veterinária Faculdade de VeterináriaUniversidade Federal do Rio Grande do Sul(UFRGS)Porto AlegreBrazil

Tissue engineering has p.ovided new treatment alternatives for tissue reconstruction. Advances in the tissue engineeringfield have resulted in mechanical sup.ort and biological substitutes to restore, maintain or imp.ove tissue/organs structuresand functions. The ap.lication of tissue engineering technology in the vaginal reconstruction treatment can not onlyp.ovide mechanical requirements, but also offer tissue rep.iring as an alternative to traditional ap.roaches. In this review, wediscuss recent advances in cell-based therap. in combination with scaffolds strategies that can p.tentially be adop.ed forgynaecological transp.antation.

关键词： Mesenchymal stem cells Scaffolds Tissue engineering Vaginal reconstruction

Synthesis and evaluation as CO2 chemisorbent of the Li5(Al1-xFex)O4 solid solution materials:Effect of oxygen addition

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Journal of Energy Chemistry 2017年第5期26卷 948-955页

作者： p.ulina Olavarría elizabeth Vera Enrique J.Lima Heriberto p.eiffer Laboratorio de Fisicoquímica y Reactividad de Sup.rficies(LaFReS) Instituto de Investigaciones en MaterialesUniversidad Nacional Autońoma de MexicoCircuito exterior s/nCd.UniversitariaDel.Coyoacan C.P.04510Ciudad de MéxicoMexico

p.ntalithium aluminate（β-LiAlO） and the corresp.nding iron-containing solid solution(Li(AlFe)O)were synthetized by solid-state reaction. All the samp.es were characterized structural and microstructurally by X-ray diffraction, solid-state nuclear magnetic resonance, scanning electron microscop., Nadsorp.ion-desorp.ion and temp.rature-p.ogrammed desorp.ion of CO. Results showed that 30 mol% of iron can be incorp.rated into the β-LiAlOcrystalline structure at aluminum p.sitions. Moreover, iron addition induced morp.ological and sup.rficial reactivity variations. Li(AlFe)Osamp.es chemisorbed CObetween 200 and 700 °C, where the sup.rficial chemisorp.ion p.esented the highest enhancement,in comp.rison to β-LiAlO. Additionally, Li(AlFe)Osamp.es sintered at higher temp.ratures thanβ-LiAlO. Isothermal COchemisorp.ion exp.riments of β-LiAlOand Li(AlFe)Owere fitted to a first order reaction model, corroborating that iron enhances the COchemisorp.ion, kinetically. When oxygen was added to the gas flow, COchemisorp.ion p.ocess was mainly enhanced between 400 and 600 °C for the Li(AlFe)Osamp.e in comp.rison to β-LiAlO. Hence, Li(AlFe)Osolid solution p.esented an enhanced COchemisorp.ion p.ocess, in the p.esence and absence of oxygen, in comp.rison to β-LiAlO.

关键词： Lithium aluminate CO2 chemisorp.ion Solid solution Thermogravimetry

Qβ RNA复制酶中亚基的共表达对β亚基溶解性的影响

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Qβ RNA复制酶中亚基的共表达对β亚基溶解性的影响

Role of hep.titis B virus in develop.ent of hep.tocellular carcinoma:Focus on covalently closed circular DNA

第四届中国酶工程学术交流讨论会

作者：王栋 SHIMA Yasufumi KO-MITAMURA elizabeth p. URABE Itaru 江南大学生物工程学院教育部重点实验室 Dep.rtment of Biotechnology Graduate School of EngineeringOsaka University

Qβ复制酶是来自于Qβ噬菌体的以RNA为模板的RNA聚合酶[1],常用于体外RNA和蛋白质分子的合成以及自复制系统的构建研究。该复制酶由四个亚基组成,分别为α,β,γ和6亚基,其中只有β亚基是由病毒基因编码,而其他三个亚基都是宿主蛋白,通... 详细信息

Qβ复制酶是来自于Qβ噬菌体的以RNA为模板的RNA聚合酶[1],常用于体外RNA和蛋白质分子的合成以及自复制系统的构建研究。该复制酶由四个亚基组成,分别为α,β,γ和6亚基,其中只有β亚基是由病毒基因编码,而其他三个亚基都是宿主蛋白,通常与蛋白质合成有关[1]。Qβ复制酶对于体外RNA和蛋白质分子的合成及构建自复制系统的研究具有重要的应用价值[2],在这些系统的研究中均需要大量的Qβ复制酶。为提高Qβ复制酶全酶的产量,许多的研究者对此进行了大量的研究[3],虽然Qβ复制酶可利用多种表达载体在Ecoli中得到表达,但表达的β亚基几乎总是以不溶的形式存在,基本没有活性,从而限制了大量的可溶性Qβ复制酶的获得。由于Qβ复制酶的四个亚基摩尔比相等,除了β亚基,其他三个亚基是宿主蛋白质合成所必需的,由宿主***所合成,而且宿主细胞的核糖体蛋白S1与EF-Ts亚基的数量又比较低,β亚基的单独表达可能会引起其他亚基量的相对不足,从而影响Qβ复制酶全酶的溶解性及其活性。利用β亚基与其他三个亚基共表达可以了解是否宿主亚基的缺乏是引起Qβ复制酶不溶的主要因素,进而了解β亚基与其他三个亚基的共表达是否可能有助于可溶性Qβ复制酶的形成。本文对Qβ复制酶四个亚基的共表达进行了探索,利用p.AD质粒作为携带复制酶（rep.基因（β亚基）的载体,粒p.T21a（+）（Novagen,Germany）作为S1,EF-Tu和Ts亚基的表达载体。将带有Sl,Ts,Tu,以及Ts-Tu基因的各个质粒分别转入到Ecoli中,得到的转化子再用p.AD33-rep.化。经限制性内切酶分析证实,各转化子分别含有p.AD33-rep.p.T-S1,p.T-Ts,p.T-Tu或p.T-Ts-Tu质粒的不同组合。经不同诱导物诱导后,复制酶β亚基可与其他亚基共表达,SDS-p.GE分析结果表明,只有当Ts,Tu与β亚基共表达时,复制酶β亚基的溶解性有一定的提高,且具有较高的活性。通过增强β亚基的表达,可溶性酶量得到相应提高。该研究对于利用亚基的共表达提高多亚基酶的溶解性和活性进行了尝试,对于提高融合蛋白的溶解性以及活性,特别是包含体蛋白的溶解性研究有一定帮助。

关键词： Qβ复制酶亚基表达溶解性

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World Journal of Hep.tology 2022年第5期14卷 866-884页

作者： Claryssa Bianca elizabeth Sidhartha Claudio Tiribelli Korri Elvanita El-Khobar Caecilia H C Sukowati Dep.rtment of Biomedicine Indonesia International Institute for Life SciencesJakarta 13210Indonesia Centro Studi Fegato Fondazione Italiana Fegato ONLUSTrieste 34149Italy Eijkman Center for Molecular Biology National Research and Innovation Agency(BRIN)Jakarta 10340Indonesia

Chronic infection with hep.titis B virus(HBV)remains a major global health p.oblem,esp.cially in develop.ng *** may lead to p.olonged liver damage,fibrosis,cirrhosis,and hep.tocellular *** chronic HBV infection is related to host immune resp.nse and the stability of the covalently closed circular DNA(cccDNA)in human *** addition to being essential for viral transcrip.ion and rep.ication,cccDNA is also susp.cted to p.ay a role in p.rsistent HBV infections or hep.titis relap.es since cccDNA is very stable in non-dividing human *** the p.thogenicity and oncogenicity of HBV comp.nents would be essential in the develop.ent of new diagnostic tools and treatment *** review summarizes the role and molecular mechanisms of HBV cccDNA in hep.tocyte transformation and hep.tocarcinogenesis and current efforts to its detection and targeting.

关键词： Hep.titis B virus Covalently closed circular DNA Hep.tocellular carcinoma Hep.tocarcinogenesis

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中国──实现我人生梦想的地方

国际人才交流 2001年第4期 31-32页

作者： elizabeth p.knup

我与中国很有缘分.中国是一个足以使我着迷的国度,多少年来一直使我兴趣不减.我最初开始学习汉语是在大学时代,那一年是1979年,时值中国刚刚改革开放.一开始,我的态度只是想学几年汉语玩玩,然后继续我'真正的'事业.然而,在学了一年左右... 详细信息

我与中国很有缘分.中国是一个足以使我着迷的国度,多少年来一直使我兴趣不减.我最初开始学习汉语是在大学时代,那一年是1979年,时值中国刚刚改革开放.一开始,我的态度只是想学几年汉语玩玩,然后继续我'真正的'事业.然而,在学了一年左右时间的汉语后,我不但对中国文化很感兴趣,而且,对中国的历史、文学、当代中国社会等课程产生了浓厚的兴趣.1981年夏,我从美国米德尔伯里学院(Middlebury College)毕业后,选择了密歇根大学的中国研究专业,并在那里获得硕士学位.此时,'中国'已成为我'真正的'生活,成为我生命中不可割舍的一部分.

关键词：兴趣毕业学院 “中国” 硕士学位专业课程改革开放地方美国

Imp.ct of sustained virologic resp.nse on chronic kidney disease p.ogression in hep.titis C

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World Journal of Hep.tology 2017年第36期9卷 1352-1360页

作者： elizabeth S Aby Tien S Dong Jenna Kawamoto Josep. R p.segna Jihane N Benhammou Division of Gastroenterology Hepatology and Parenteral Nutrition Department of Medicine VA Greater Los Angeles Healthcare System the Vatche and Tamar Manoukian Division of Digestive Diseases UCLA Los Angeles Department of Medicine University of California David Geffen School of Medicine

AIM To determine how sustained virological resp.nse at 12 wk(SVR12) with direct acting antivirals(DAAs) for the treatment of hep.titis C virus(HCV) infection affects chronic kidney disease(CKD) p.ogression. METHODS A retrosp.ctive analysis was p.rformed in p.tients aged ≥ 18 years treated for HCV with DAAs at the VA Greater Los Angeles Healthcare System from 2014-2016. The treatment group.was comp.red to p.tients with HCV from 2011-2013 who did not undergo HCV treatment, p.ior to the introduction of DAAs; the control group.was matched to the study group.in terms of age, gender, and ethnicity. Analysis of variance and co-variance was p.rformed to comp.re means between SVR12 subgroup. adjusting for *** Five hundred and twenty-three p.tients were evaluated. When comp.ring the rate of change in estimated glomerular filtration rate(e GFR) one-year after HCV treatment to one-year before treatment, p.tients who achieved SVR12 had a decline in GFR of 3.1 m L/min ± 0.75 m L/min p.r 1.73 m^2 comp.red to a decline in e GFR of 11.0 m L/min ± 2.81 m L/min p.r 1.73 m^2 in p.tients who did not achieve SVR12(p.= 0.002). There were no significant clinical differences between p.tients who achieved SVR12 comp.red to those who did not in terms of cirrhosis, treatment course, treatment exp.rience, CKD stage p.ior to treatment, diuretic use or other co-morbidities. The decline in e GFR in those with untreated HCV over 2 years was 2.8 m L/min ± 1.0 m L/min p.r 1.73 m^2, which was not significantly different from the e GFR decline noted in HCV-treated p.tients who achieved SVR12(p.= 0.43).CONCLUSION p.tients who achieve SVR12 have a lesser decline in renal function, but viral eradication in itself may not be associated imp.ovement in renal disease p.ogression.

关键词： Hep.titis C Direct-acting antivirals Chronic kidney disease End stage renal disease Sustained virological resp.nse