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Analysis of genetic relationship among Arbutus unedo L. genotypes using RAPD and SSR markers

Journal of Forestry Research 2013年第2期24卷 227-236页

作者： Filomena Gomes Rita Costa Maria M. Ribeiro elisa figueiredo Jorge M. Canhoto CERNAS Departamento Florestal Escola Superior Agrária Coimbra INRB Instituto Nacional de Recursos Biológicos Departamento de Recursos Naturais e Desenvolvimento Sustentável Escola Superior Agrária Centre of Functional Ecology Department of Life Sciences University of Coimbra

The strawberry tree （Arbutus unedo L.） is an underutilized, drought tolerant, fire resistant species with a south western distribution in Europe, and with ecological and putative socio-economical impact in Portugal and Mediterranean countries. Our aim was to develop an appropriate set of molecular markers to enable genetic diversity to be assessed and to fingerprint Arbutus unedo genotypes for breeding and conservation purposes in Portugal. Twenty-seven trees from a broad geographic range were screened with 20 random amplified polymorphic DNA （RAPD primers） and 11 microsatellite markers （SSR）. The RAPDs generated 124 bands, 57.3% of which were polymorphic, with an expected heterozygosity of 27%. We cross-amplified 11 SSR primers developed for Vaccinium spp., and 5 were found to be polymorphic in A. unedo, with 75% of expected heterozygosity, a number of alleles of 11.6, a null allele frequency of 7.6% and a polymorphic information content of 71%. Although the SSRs were more polymorphic and informative than the RAPDs, both markers displayed high genetic variability with the gathered data. No geographic pattern was observed in the genetic variation distribution based on both marker systems, and the lack of correlation between genetic and geographical matrices was confirmed by Mantel tests. Likely, no correlation was found between pairwise SSR and RAPD band-sharing matrices. These results and their implications on A. unedo breeding and conservation programs are discussed.

关键词： Ericaceae fingerprinting geographic pattern molecular markers strawberry tree.

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Janus-faced Mitofusin 2 (MFN2): mitochondria-endoplasmic reticulum shaping and tethering functions unveiled

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Signal Transduction and Targeted Therapy 2024年第2期9卷 341-342页

作者： Camilla Aurora Franchino elisa Motori Matteo Bergami Institute for Biochemistry University of Cologne50674 CologneGermany Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases(CECAD) Faculty of Medicine and University Hospital CologneUniversity of Cologne50931 CologneGermany Center for Molecular Medicine 50931 CologneGermany

In a recently published study in Science,Naòn et *** two uncharacterized splice variants of Mitofusin 2(MFN2)that specifically shape and tether the endoplasmic reticulum(ER)to mitochondria.1 This work sheds new light on the pleiotropic effects previously ascribed to MFN2 loss-of-function mutations,and has important implications for associated metabolic and neurological diseases.

关键词： MFN2 reticulum

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Climate change impacts on eutrophication in the Po River(Italy):Temperature-mediated reduction in nitrogen export but no effect on phosphorus

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Journal of Environmental Sciences 2024年第9期143卷 148-163页

作者： elisa Soana Maria Pia Gervasio Tommaso Granata Daniela Colombo Giuseppe Castaldelli Department of Environmental and Prevention Sciences University of FerraraVia Luigi Borsari 4644121 FerraraItaly CESI-Italian Electrical and Technical Experimental Center via Rubattino 5420134MilanoItaly

Rivers worldwide are under stress from eutrophication and nitrate pollution,but the ecological consequences overlap with climate change,and the resulting interactions may be unexpected and still *** Po River basin(northern Italy)is one of the most agriculturally productive and densely populated areas in *** remains unclear whether the climate change impacts on the thermal and hydrological regimes are already affecting nutrient dynamics and transport to coastal *** present work addresses the long-term trends(1992-2020)of nitrogen and phosphorus export by investigating both the annual magnitude and the seasonal patterns and their relationship with water temperature and discharge *** the constant diffuse and point sources in the basin,a marked decrease(-20%)in nitrogen export,mostly as nitrate,was recorded in the last decade compared to the 1990 s,while no significant downward trend was observed for *** water temperature of the Po River has warmed,with the most pronounced signals in summer(+0.13℃/year)and autumn(+0.16℃/year),together with the strongest increase in the number of warm days(+70%-80%).An extended seasonal window of warm temperatures and the persistence of low flow periods are likely to create favorable conditions for permanent nitrate removal via denitrification,resulting in a lower delivery of reactive nitrogen to the *** present results show that climate change-driven warming may enhance nitrogen processing by increasing respiratory river metabolism,thereby reducing export from spring to early autumn,when the risk of eutrophication in coastal zones is higher.

关键词： Nutrient export Eutrophication Climate change Water temperature Denitrification Po River

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Recurrent evolution of seaweed body plan complexity among photosynthetic eukaryotes

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Molecular Plant 2024年第7期17卷 999-1001页

作者： elisa S.Goldbecker Iker Irisarri Jan de Vries Department of Applied Bioinformatics Institute for Microbiology and GeneticsUniversity of GottingenGoldschmidtstr.137077 GottingenGermany Section Phylogenomics Center for Molecular Biodiversity ResearchLeibniz Institute for the Analysis of Biodiversity Change(LIB)Museum of Nature HamburgMartin-Luther-King-Platz 320146 HamburgGermany Department of Applied Bioinformatics Campus Institute Data Science(CIDAS)University of GottingenGoldschmidtstr.137077 GottingenGermany Department of Applied Bioinformatics Gottingen Center for Molecular Biosciences(GZMB)University of GottingenGoldschmidtstr.137077 GottingenGermany

The evolutionary origin of multicellularity is an age-old question in biological *** studies have delved into the evolutionary processes that led to the evolution of multicellular life forms from unicellular *** beauty and the challenge about this topic are that evolution has brought forth various forms of multicellular life,multiple times independently,scattered across the evolutionary tree of *** to the challenge,multicellular organisms exist in a diversity of forms and complexities,and the mechanisms by which multicellularity emerged are also likely different between animals,algae,or fungi(Niklas and Newman,2020).Recently,Nelson et al.(2024)presented a large-scale comparative genomics study focused on particularly intricate multicellular organisms:macroalgae(seaweeds),which emerged independently in three distinct eukaryotic lineages-green algae(Chlorophyta),red algae(Rhodophyta),and brown algae(Phaeophyceae).

关键词： algae seaweed evolution

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Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment

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Marine Life Science & Technology 2024年第1期6卷 126-142页

作者： elisa Banchi Erwan Corre Paola Del Negro Mauro Celussi Francesca Malfatti National Institute of Oceanography and Applied Geophysics OGS TriesteItaly FR2424 Station Biologique de RoscoffPlateforme ABiMS(Analysis and Bioinformatics for Marine Science)Sorbonne Universite CNRS29680 RoscoffFrance Department of Life Sciences University of TriesteTriesteItaly

Bacteria living in sediments play essential roles in marine ecosystems and deeper insights into the ecology and biogeochemistry of these largely unexplored organisms can be obtained from‘omics’***,we characterized metagenome-assembled-genomes(MAGs)from the surface sediment microbes of the Venice Lagoon(northern Adriatic Sea)in distinct sub-basins exposed to various natural and anthropogenic *** were explored for biodiversity,major marine metabolic processes,anthropogenic activity-related functions,adaptations at the microscale,and biosynthetic gene *** from 126 MAGs,a non-redundant dataset of 58 was compiled,the majority of which(35)belonged to(Alpha-and Gamma-)*** the broad microbial metabolic repertoire(including C,N,and S metabolisms)the potential to live without oxygen emerged as one of the most important *** was also found as a successful *** analysis showed that different MAGs encoded the same metabolic patterns(e.g.,C fixation,sulfate oxidation)thus suggesting metabolic *** and toxic compounds resistance genes were coupled,a condition that could promote the spreading of these genetic *** showed a high biosynthetic potential related to antimicrobial and biotechnological classes and to organism defense and interactions as well as adaptive strategies for micronutrient uptake and cellular *** results highlighted that bacteria living in an impacted environment,such as the surface sediments of the Venice Lagoon,may benefit from metabolic plasticity as well as from the synthesis of a wide array of secondary metabolites,promoting ecosystem resilience and stability toward environmental pressures.

关键词： Antibiotic resistance PAHs Biosynthetic gene clusters Microscale Mobilome Resistome

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Quasilinear elliptic problems with exponential growth via the Nehari manifold method: Existence of nonnegative and nodal solutions

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Acta Mathematica Sinica,English Series 2024年

作者： Giovany figueiredo Sandra Moreira Ricardo Ruviaro Address Departamento de Matematica Universidade de Brasilia Departamento de Matematica e Informatica Universidade Estadual do Maranhao Departamento de Matematica Universidade de Brasilia

In this paper we will be concerned with the problem -Δu-1/2Δ(a（x）u2)u+V（x）u=f（u）,x∈R2，where V is a potential continuous and f : R → R is a superlinear continuous function with exponential subcritical or exponential critical growth. We use as a main tool the Nehari manifold method in order to show existence of nonnegative solutions and existence of nodal solutions. Our results complement the classical result of solutions for quasilinear Schrodinger equations via the Nehari method due to Jia-quan Liu, Ya-qi Wang and Zhi-Qiang Wang in the sense that in this article we are considering nonlinearity of the exponential type.

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Reviving Ties

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Beijing Review 2024年第35期67卷 24-25页

作者： elisa Tirabassi Swiss Radiotelevision Italian Language

I talian Prime Minister Giorgia Meloni's official visit to China from July 27 to 31 was the first by a Western leader since the Third Plenary Session of the 20th Central Committee of the Communist Party of China(CPC),... 详细信息

关键词： comprehensive strategic official

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Inflammation as a cause of acute myocardial infarction in patients with myeloproliferative neoplasm

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World Journal of Cardiology 2024年第2期16卷 58-63页

作者： Amedeo Tirandi elisa Schiavetta Elia Maioli Fabrizio Montecucco Luca Liberale Department of Internal Medicine University of GenoaGenoa 16132Italy IRCCS Ospedale Policlinico San Martino Genoa–Italian Cardiovascular NetworkGenoa 16132Italy

Myeloproliferative neoplasms(MPN)are a group of diseases characterized by the clonal proliferation of hematopoietic progenitor or stem *** are clinically classifiable into four main diseases:chronic myeloid leukemia,essential thrombocythemia,polycythemia vera,and primary *** pathologies are closely related to cardio-and cerebrovascular diseases due to the increased risk of arterial thrombosis,the most common underlying cause of acute myocardial *** evidence shows that the classical Virchow triad(hypercoagulability,blood stasis,endothelial injury)might offer an explanation for such ***,patients with MPN might have a higher number and more reactive circulating platelets and leukocytes,a tendency toward blood stasis because of a high number of circulating red blood cells,endothelial injury or overactivation as a consequence of sustained inflammation caused by the neoplastic clonal *** abnormal cancer cells,especially when associated with the JAK2V617F mutation,tend to proliferate and secrete several inflammatory *** sustains a pro-inflammatory state throughout the *** direct consequence is the induction of a pro-thrombotic state that acts as a determinant in favoring both venous and arterial thrombus ***,MPN patients need to be carefully evaluated to be treated not only with cytoreductive treatments but also with cardiovascular protective strategies.

关键词： Inflammation Myeloproliferative neoplasm Acute coronary syndrome Myocardial infarction Thrombosis Cancer

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Enhanced Solar Water Desalination by CuCo_(2)S_(4)-decorated Carbon Foam Derived from Waste Plastics

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Chemical Research in Chinese Universities 2024年第3期40卷 548-555页

作者： Muzammil HUSSAIN Tofik Ahmed SHIFA Pratik V.SHINDE Pankaj KUMAR Stefano CENTENARO Silvia GROSS elisa MORETTI Alberto VOMIERO Department of Molecular Sciences and Nanosystems Ca'Foscari University of VeniceVia Torino 15530172 VeniceItaly Department of Industrial Engineering University of PadovaVia Venezia 135131 PadovaItaly Department of Chemical Sciences University of PadovaVia Francesco Marzolo135131 PadovaItaly Division of Materials Science Department of Engineering Sciences and MathematicsLulea University of Technology97187 LuleaSweden Center for Cultural Heritage Technologies(CCHT) Istituto Italiano di Tecnologia(IT)Via Torino 15530170 Venezia-MestreItaly

Interfacial solar desalination is an emerging technology for freshwater production,but the finding of novel solar evaporators is still *** the present research,graphitic carbon foam(CF)was synthesized from the upcycling of waste plastic polyethylene terephthalate(PET)waste bottles functionalized with carrollite CuCo_(2)S_(4) as a photothermal *** characterization[X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),and scanning electron microscopy-energy dispersive spectroscopy(SEM-EDS)]confirms the functionalization of carrollite CuCo_(2)S_(4) on graphitic carbon *** UV-Vis spectroscopy analysis showed an enhanced optical absorption in the UV-Vis-near IR region(>96%)for functionalized CuCo_(2)S_(4)-CF foam compared to carbon foam(67%).The interfacial solar desalination experiment presented a significantly enhanced evaporation rate of 2.4 kg·m^(−2)·h^(−1) for CuCo_(2)S_(4)-CF compared to that of CF(1.60 kg·m^(−2)·h^(−1))and that of CuCo_(2)S_(4)(1.60 kg·m^(−2)·h^(−1)).The obtained results proved that the newly synthesized CuCo_(2)S_(4)-CF from the upcycled plastic into new material for the photothermal desalination process can enhance the practice of a circular economy to produce fresh water.

关键词： Photothermal desalination Cobalt Copper Chalcogenide Waste upcycling Circular economy

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Hydrogeochemical and isotopic assessment of the origin of NO_(3)^(−)and N-NH_(3)contents in the aquifer located in a closed lacustrine volcano-sedimentary basin in the metropolitan area of Mexico City

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Acta Geochimica 2024年第4期43卷 719-736页

作者： Jose Ivan Morales-Arredondo Maria Aurora Armienta Hernandez Joel Edmundo Ortega-Gutierrez elisa Cuellar Ramirez Natural Resources Department Institute of GeophysicsNational Autonomous University of MexicoCiudad Universitaria No.3000Col.Copilco UniversidadCoyoacán Delegation04360 MexicoCDMXMexico Instituto Politecnico Nacional ***án 55La Laguna Ticoman07340 MexicoCDMXMexico Benemérita Universidad Autónoma de Puebla Ciudad Universitaria Col.San ManuelPueblaPueMexico

To explain the presence and spatial distribution of NO_(3)^(−)and N-NH_(3)in the Aquifer of the Metropolitan Area of Mexico City(AMAMC),a hydrogeochemical and isotopic analysis using^(13)C DIC(as well as the stable isotopes^(18)O and^(2)H)in groundwater was *** aquifer is located in an old closed lacustrine volcano-sedimentary basin;some wells hosted in the semi-confined zone contain high N-NH_(3)concentrations,while others present NO_(3)^(−)contents in the recharge zones(hosted in an oxidizing environment).In this study,a change in the isotopic signature(primarily in^(18)O and^(2)H)was observed from the recharge zones to the basin center in some of the wells with high NO_(3)^(−)concentrations,this behavior can be attributed to evaporation during the incorporation of recently infiltrated *** addition,the results for^(13)C(along with ^(2) H)in wells with the highest N-NH_(3)concentrations exhibited an atypically broad range of *** indicated the occurrence of hydrogeochemical and/or biochemical processes in the aquifer(in an oxidizing or reducing environment),such as organic degradation,bacterial decomposition(primarily in the ancient Lake Texcoco and which acts as a natural sink for carbon,nitrogen,sulfur,and phosphorus),besides rock weathering and dissolution,which may be responsible for a very marked isotopic modification of the^(13)C(and,to a lesser extent,2 H).Methanotrophic bacterial activity and methanogenic activity may be related to N-NH_(3)removal processes by oxidation and residual water incorporation respectively,whereas the increase in the NO_(3)^(−)content in some wells is due to the recent contribution of poor-quality water due to contamination.

关键词： 18 O and^(13)C isotopes Iron Hydrogeochemical processes Nitrate Nitrogen species

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