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First Characterization of <i>Escherichia coli</i>Strains Isolated from Wildlife Griffon Vulture (<i>Gyps fulvus</i>) in the Southeast of Spain

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Open Journal of Veterinary Medicine 2014年第12期4卷 329-333页

作者： Azucena Mora Nieves Ortega elena garcia Susana Viso Mónica G. Candela Ghizlane Dahbi Francisco Cuello María Rosa Caro Laboratorio de Referencia de E. coli (LREC) Departamento de Microbiología y Parasitología Facultad de Veterinaria Universidad de Santiago de Compostela Lugo Spain Departamento de Sanidad Animal Facultad de Veterinaria Campus de Espinardo Universidad de Murcia Murcia Spain

The aim of the present study was to characterize a collection of Escherichia coli strains isolated from asymptomatic griffon vulture (Gyps fulvus) during a reintroduction program in the southeast of Spain, in order to establish if griffon vulture could play a role in the spread of resistant or potentially pathogenic E. coli strains. For this purpose, 14 E. coli strains obtained from 10 griffon vulture were studied to establish their serotypes, phylogroups, virulencegene profiles and antimicrobial resistances. High heterogeneity was observed within the 14 strains isolated which belonged to three phylogroups (A, B1 and D), 8 serogroups (O2, O21, O29, O60, 073, O78, O103 and O141) and 13 different serotypes. Out of 34 genes screened, we have detected eight virulence genes that are typical of extraintestinal pathogenic E. coli (ExPEC) (fimH, fimAvMT78, iroN, iucD, cvaC, iss, traT and tsh);however, none of the studied strains showed the ExPEC status. The 14 strains were also analyzed for the production of extended-spectrum beta-lactamases (ESBLs) and for antimicrobial resistances. None of the 14 strains were ESBL-producing E. coli, but high resistance-prevalences to ampicillin and cotrimoxazole were detected. To our knowledge, this is the first characterization of E. coli strains isolated from griffon vulture and although they did not show high virulencegene scores, they showed cotrimoxazole resistance.

关键词： Antibiotic Resistance APEC Escherichia coli ExPEC Griffon Vulture

Successful hip revision surgery following refracture of a modern femoral stem using a cortical window osteotomy technique:A case report and review of literature

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World Journal of Methodology 2023年第5期13卷 502-509页

作者： Carlos M Lucero Juan B Luco Agustin garcia-Mansilla Pablo A Slullitel Gerardo Zanotti Fernando Comba martin A Buttaro Centro de Cadera"Sir John Charnley" Instituto de Ortopedia y Traumatologia Prof.Dr.Carlos E.OttolenghiHospital Italiano de Buenos AiresCapital federal C1198AAWBuenos AiresArgentina Instituto de Ortopedia y Traumatologia Prof.Dr.Carlos E.Ottolenghi Hospital Italiano de Buenos AiresCiudad Autonoma de Buenos Aires C1198AAWBuenos AiresArgentina Centro de Cadera"Sir John Charnley" Instituto de Ortopedia y Traumatología“Prof.Dr.Carlos E.Ottolenghi”Hospital Italiano de Buenos AiresCiudad Autónoma de Buenos Aires C1198AAWBuenos AiresArgentina

BACKGROUND The ExeterTM Universal cemented femoral component is widely used for total hip replacement *** there have been few reports of femoral component fracture,removal of a broken femoral stem can be a challenging *** SUMMARY A 54-year-old man with a Dorr A femur sustained a refracture of a primary ExeterTM stem,two years after receiving a revision using a cement-within-cement technique(CWC)through an extended trochanteric osteotomy(ETO).The technical problems related to the CWC technique and the ETO played a major role in the stem fatigue *** performed revision surgery and removed the distal cement using a cortical femoral window technique,followed by reimplantation with an uncemented,modular,distally-fixed uncemented *** patient experienced an uneventful postoperative *** Re-fracture of a modern femoral ExeterTM stem is a rare event,but technical complications related to revision surgery can lead to this *** cortical window osteotomy technique can facilitate the removal of a broken stem and cement,allowing for prosthetic reimplantation under direct vision and avoiding ETO-related complications.

关键词： Exeter stem Femoral stem breakage Femoral osteotomy Total hip arthroplasty Case seport

Reciprocal Effects of Plant-Soil Relationships at Two Spatial Scales in a Semiarid Land of Central Mexico

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Journal of Agricultural Science and Technology(A) 2012年第1期2卷 71-79页

作者： Francisco martin Huerta-martinez Alejandro Mufioz-Urias Cecilia Neri-Luna Claudia Aurora Uribe-Mu Edmundo garcia-Moya Departamento de Ecologia Centro Universitario de Ciencias Biol6gicas y Agropecuarias Universidad de Guadalajara Zapopan Jalisco C.P. Mexico Instituto de Recursos Naturales Colegio de Postgraduados Montecillo Edo. de Mexico C.P 56230 Mexico

We studied the influence of soil heterogeneity on plant community structure in a semiarid region of Central Mexico using Bray-Curtis Ordination. The results showed that some edaphic factors, such as soil depth, organic matter, and potassium and calcium content, explained 80% of the total variation in structure of the studied communities. We found that soil resources were heterogeneously distributed in the study area, indicating that the edaphic variables considered in this study explain the existing plant community variability, moreover the presence of some shrubs as Krameria cytisoides influences the soil properties, suggesting that there is a reciprocal effect between plant and soil.

关键词： Ordination vegetation soil Chihuahuan Desert.

Albumin-bound paclitaxel as new treatment for metastatic cholangiocarcinoma: A case report

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World Journal of Clinical Oncology 2020年第10期11卷 844-853页

作者： Roberto martin Huertas Raquel Fuentes-Mateos Juan Jose Serrano Domingo elena Corral de la Fuente Mercedes Rodríguez-Garrote Medical Oncology Department Hospital Universitario Ramon y CajalMadrid 28034Spain

BACKGROUND Cholangiocarcinomas are rare and very aggressive *** patients have advanced-stage or unresectable disease at presentation,and the systemic therapies have limited ***-bound paclitaxel(nab-paclitaxel)is a solvent-free taxane that has been approved for the treatment of some cancers such as breast,non-small cell lung and pancreatic cancer,however it has not been applied to treat *** have both preclinical and clinical evidence of the efficacy of nab-paclitaxel in cholangiocarcinoma,yet no phase 3 trials have been *** SUMMARY A 63-year-old man was diagnosed in December 2016 with stage III B intrahepatic *** was performed,followed by adjuvant chemotherapy treatment with capecitabine and gemcitabine;although,the gemcitabine was suspended due to allergic reaction after two *** April 2019,metastatic cholangiocarcinoma relapse was diagnosed,and a first-line treatment with FOLFOX scheme was *** cycles were administered,producing an initial clinical improvement and decrease in blood tumor marker *** and serological progression was noted in September *** a second-line treatment,FOLFIRI was not recommended due to risk of worsening the patient’s tumor-related diarrhea.A combination therapy with gemcitabine was not feasible,as the patient had previously suffered from an allergic reaction to this *** decided to use nab-paclitaxel as a second-line treatment,and four cycles were *** clinical and serological responses were observed,and a radiological mixed response was also *** Advanced cholangiocarcinoma could be treated with nab-paclitaxel monotherapy,which should be studied in combination with other types of treatment(chemotherapy,fibroblast growth factor receptor inhibitors).

关键词： Cholangiocarcinoma Chemotherapy Albumin-bound paclitaxel Case report Metastatic Clinical trial

Optical magnetic field enhancement using ultrafast azimuthally polarized laser beams and tailored metallic nanoantennas

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Photonics Research 2024年第5期12卷 1078-1092页

作者： RODRIGO martin-HERNANDEZ LORENZ GRUNEWALD LUIS SANCHEZ-TEJERINA LUIS PLAJA ENRIQUECONEJERO JARQUE CARLOS HERNANDEZ-garcia SEBASTIAN MAI Grupo de Investigacion en Aplicaciones del Laser y Fotonica Departamento de Fisica AplicadaUniversidad de SalamancaE-37008 SalamancaSpain Unidad de Excelencia en Luz y Materia Estructuradas(LUMES) Universidad de SalamancaE-37008 SalamancaSpain Institute of Theoretical Chemistry Faculty of ChemistryUniversity of Vienna1090 ViennaAustria Vienna Doctoral School in Chemistry(DoSChem) Faculty of ChemistryUniversity of Vienna1090 ViennaAustria Departamento de Electricidad y Electrónica Universidad de Valladolid47011 ValladolidSpain

*** *** thank Valentina Shumakova,Elizaveta Gangrskaia,Alessandra Bellissimo,Audrius Pugzlys,and Andrius Baltu?ka for fruitful discussions and computational support by the Vienna Scientific Cluster(VSC 4).The authors thankfully acknowledge the computer resources at MareNostrum and SCAYLE,and the technical support provided by Barcelona Supercomputing Center(RES-FI-2022-3-0041).R.M.-H.,L.S.-T.,L.P.,E.C.J.,and C.H.-*** funding from the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation program(Grant Agreement No.851201)and support from Ministerio de Ciencia e Innovación(PID2022-142340NB-I00,PID2019-106910GB-I00).This research was funded in whole or in part by the Austrian Science Fund(FWF)(10.55776/ZK91).For open access purposes,the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission.

关键词： metallic polarized discussions

领导者–跟随者Thomson跳环系统的自抗扰控制设计(英文)

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控制理论与应用 2013年第12期30卷 1564-1572页

作者： Mario RAMIREZ-NERIA José L garcia-ANTONIO Hebertt J SIRA-RAMIREZ martin VELASCO-VILLA Rafael CASTRO-LINARES Depto. de Control Automatico Cinvestav Av. IPN 2508 C.E 07360 Mexico D.F. Seccion de Mecatronica Depto. de Ingenieria Electrica Cinvestav Av. IPN 2508 C.E 07360 Mexico D.E

将用电磁方法悬浮起来的一对环构成领导者–跟随者系统实现对光滑参考轨线的跟踪要求.这一任务通过设计具有对扰动进行在线估计与补偿功能的自抗扰控制器(ADRC)实现.本文设计方法与传统ADRC的主要不同在于领导者和跟随者的跟踪控制器设... 详细信息

将用电磁方法悬浮起来的一对环构成领导者–跟随者系统实现对光滑参考轨线的跟踪要求.这一任务通过设计具有对扰动进行在线估计与补偿功能的自抗扰控制器(ADRC)实现.本文设计方法与传统ADRC的主要不同在于领导者和跟随者的跟踪控制器设计都是基于平坦输出的线性化近似系统.超出线性化近似有效区域的大的高度偏差所导致的未知非线性则被视为扰动,这个扰动借助于线性扩张观测器进行在线估计并通过线性反馈控制器进行消除.实验结果检验了本文所提方法的有效性.

关键词： Thomson跳环自抗扰控制广义比例积分(GPI)观测器领导者-跟随者布局

Usefulness of the CONUT index upon hospital admission as a potential prognostic indicator of COVID-19 health outcomes

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Chinese Medical Journal 2022年第2期135卷 187-193页

作者： Adrian K.Bengelloun Guillermo J.Ortega Julio Ancochea Ancor Sanz-garcia Diego A.Rodriguez-Serrano Guillermo Fernandez-Jimenez Rosa Giron elena Avalos Joan B.Soriano J.Ignacio de Ulibarri Facultad de Medicina Universidad Autónoma de MadridMadridSpain Unidad de Análisis de Datos Instituto de Investigación Sanitaria del Hospital de la PrincesaMadridSpain Servicios de Neumología Admisión y Documentación ClínicaCuidados Intensivos y Nutrición y DietéticaHospital Universitario de La PrincesaMadridSpain Centro de Investigación en Red de Enfermedades Respiratorias(CIBERES) Instituto de Salud Carlos III(ISCIII)MadridEspañaSpain

Background:In-hospital mortality in patients with coronavirus disease 2019(COVID-19)is *** prognostic indices are needed to identify patients at high-risk of COVID-19 health *** aimed to determine the usefulness of the CONtrolling NUTritional status(CONUT)index as a potential prognostic indicator of mortality in COVID-19 patients upon hospital ***:Our study design is of a retrospective observational study in a large cohort of COVID-19 *** addition to descriptive statistics,a Kaplan-Meier mortality analysis and a Cox regression were performed,as well as receiver operating curve(ROC).Results:From February 5,2020 to January 21,2021,there was a total of 2969 admissions for COVID-19 at our hospital,corresponding to 2844 ***,baseline(within 4 days of admission)CONUT index could be scored for 1627(57.2%)***’age was 67.3±16.5 years and 44.9%were *** CONUT severity distribution was:194(11.9%)normal(0-1);769(47.2%)light(2-4);585(35.9%)moderate(5-8);and 79(4.9%)severe(9-12).Mortality of 30 days after admission was 3.1%in patients with normal risk CONUT,9.0%light,22.7%moderate,and 40.5%in those with severe CONUT(P<0.05).An increased risk of death associated with a greater baseline CONUT stage was sustained in a multivariable Cox regression model(P<0.05).An increasing baseline CONUT stage was associated with a longer duration of admission,a greater requirement for the use of non-invasive and invasive mechanical ventilation,and other clinical outcomes(all P<0.05).The ROC of CONUT for mortality had an area under the curve(AUC)and 95%confidence interval of 0.711(0.676-0746).Conclusion:The CONUT index upon admission is potentially a reliable and independent prognostic indicator of mortality and length of hospitalization in COVID-19 patients.

关键词： Admission Clinical risk CONUT COVID-19 Prognosis Score

Endovascular treatment of paravisceral mycotic aneurysm: Chimmeny endovascular sealing the end of de road

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World Journal of Cardiology 2017年第7期9卷 629-633页

作者： martin Rabellino Pedro N Moltini Vanesa G Di Caro Jose G Chas Ricardo Marenchino Ricardo D garcia-Monaco Department of Interventional Radiology Hospital Italiano de Buenos Aires Department of Cardio-vascular Surgery Hospital Italiano de Buenos Aires

Open surgery is the elective treatment for mycotic aneurysms of the aorta. This surgery consists of resection of the aneurysm, debridement and revascularization with an in situ or extra-anatomic bypass. Even when surgery has been successful, the morbimortality is raised and the endovascular treatment has become an alternative for specific patients. When mycotic aneurysms involved the visceral arteries, more complex techniques are necessary such as fenestrated endovascular aortic repair or chimmeny endovascular aortic repair and the most frequent complications of this are endoleaks and oclussion the visceral arteries. We present a case of a pacient with a paravisceral abdominal mycotic aneurysms that was result with 2 chimney technique(in the right renal and superior mesenteric arteries) and a single Nellix EVAS(Endologix, Irvine, Calif) of 12 cm long without evidence of endoleaks in the follow-up.

关键词： Mycototic aneurysms Edovascular repair Aorta Endoleaks Para visceral aneurysms

Determining a Diagnostic Cut-Off on Fluorescence Polarization Assay(FPA)for Bovine Brucellosis in Carchi,Ecuador

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Open Journal of Animal Sciences 2017年第4期7卷 425-432页

作者： Ibarra Marcelo Benavides Hernan Salgado Ruth Gutierrez Milena garcia Judith Pena Julio Herrera David Mina Jorge Campos martin Puga Byron Agricultural Career Carchi State Polytechnic UniversityTulcánEcuador School of Veterinary Medicine and Animal Science Central University of EcuadorQuitoEcuador

Serology is the foundation of any brucellosis control and eradication program worldwide, thus it is important to define accuracy diagnostics assays and cut-off of those assays, due to variations from country to country and even among specific areas in the country. The variation of cutoff values depended on: prevalence of disease, vaccination status, animal management, and control and eradication programs. Therefore, a cut-off for the diagnosis of bovine brucellosis through fluorescence polarization assay (FPA) in Carchi—Ecuador was determined. The survey has been carried out in Carchi province of Ecuador, who is considered a province of high prevalence of brucellosis and the vaccination status is unknown due to the lack of registers. Sera samples (n = 200) were obtained from individual cows from randomly selected herds. Blood sera were tested through Fluoresce Polarization Assay (FPA) and competitive enzyme-linked inmunosorbent assay (cELISA) as confirmatory test, and then receiver operating characteristic (ROC) analysis was done. The sensitivity and specificity values of FPA were 88.7% and 92.50% respectively using a cut-off of 89.90 mP. Moreover, the area under the curve showed that 92.2% is the probability accuracy of the test. The advantage of the FPA is that it is a test with good characteristics of sensitivity and specificity as well as a simple and quick test.

关键词： Brucella Diagnosis Fluorescence Polarization Sensitivity Specificity