Determining a Diagnostic Cut-Off on Fluorescence Polarization Assay(FPA)for Bovine Brucellosis in Carchi,Ecuador
作者机构:Agricultural CareerCarchi State Polytechnic UniversityTulcánEcuador School of Veterinary Medicine and Animal ScienceCentral University of EcuadorQuitoEcuador
出 版 物:《Open Journal of Animal Sciences》 (动物科学期刊(英文))
年 卷 期:2017年第7卷第4期
页 面:425-432页
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题:Brucella Diagnosis Fluorescence Polarization Sensitivity Specificity
摘 要:Serology is the foundation of any brucellosis control and eradication program worldwide, thus it is important to define accuracy diagnostics assays and cut-off of those assays, due to variations from country to country and even among specific areas in the country. The variation of cutoff values depended on: prevalence of disease, vaccination status, animal management, and control and eradication programs. Therefore, a cut-off for the diagnosis of bovine brucellosis through fluorescence polarization assay (FPA) in Carchi—Ecuador was determined. The survey has been carried out in Carchi province of Ecuador, who is considered a province of high prevalence of brucellosis and the vaccination status is unknown due to the lack of registers. Sera samples (n = 200) were obtained from individual cows from randomly selected herds. Blood sera were tested through Fluoresce Polarization Assay (FPA) and competitive enzyme-linked inmunosorbent assay (cELISA) as confirmatory test, and then receiver operating characteristic (ROC) analysis was done. The sensitivity and specificity values of FPA were 88.7% and 92.50% respectively using a cut-off of 89.90 mP. Moreover, the area under the curve showed that 92.2% is the probability accuracy of the test. The advantage of the FPA is that it is a test with good characteristics of sensitivity and specificity as well as a simple and quick test.