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open Journal of Genetics 2021年第4期11卷 120-144页

作者： Houdou Diarra edward E. Makhulu Peter o. odhiambo Robinson M. Irekwa Johnson Kinyua Jeremy K. Herren Victor A. Mobegi Department of Molecular Biology and Biotechnology Institute of Basic Sciences Technology and Innovation Pan African University (PAUSTI) Nairobi Kenya International Centre of Insect Physiology and Ecology (ICIPE) Nairobi Kenya Nagasaki University Institute of Tropical Medicine-Kenya Medical Research Institute Project (NUITM-KEMRI-Project) Nairobi Ken-ya Department of Biochemistry Jomo Kenyatta University of Agriculture and Technology (JKUAT) Nairobi Kenya Department of Biochemistry School of Medicine University of Nairobi Nairobi Kenya

Background: Plasmodium falciparum (Pf) resistance to antimalarial drugs is a major impediment to malaria control. The Pf.Kelch 13 (PfK13) gene has been largely reported to be associated with artemisinin resistance. However, recent studies have shown artemisinin resistance without Kech13 mutations suggesting the implication of others genes in artemisinin resistance. In this current study, we focused on mutations in Pf.actin-binding protein coronin, Pf.cysteine desulfurase and Pf.plasmepsin 2 gene, three putative candidates recently were reported to be involved in artemisinin, lumefantrine and piperaquine resistance respectively. Method: Archived blood samples previously collected from asymptomatic school children from December 2016 to October 2018 were used in this study. Genomic DNA was extracted using ISOL

关键词： Plasmodium falciparum Mutations Artemisinin Lumefantrine Piperaquine

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管理者的抉择

经理人 2007年第7期 86-86,88页

作者： James o'Toole edward E. Lawler III

从年度报告和给员工的备忘录上来看，如今的CEo们大都相信，一个企业能够取得成功，在很大程度上取决于公司成员的工作热情、主动性以及责任感。可另一方面，他们却越来越多地把一些工作外包给其他公司的员工，同时开始减少本公司的员... 详细信息

从年度报告和给员工的备忘录上来看，如今的CEo们大都相信，一个企业能够取得成功，在很大程度上取决于公司成员的工作热情、主动性以及责任感。可另一方面，他们却越来越多地把一些工作外包给其他公司的员工，同时开始减少本公司的员工福利，减少终生雇用的职位，而把更多的工作交给临时工人或合同工人。为什么会出现这种情况呢？面对这个问题，CEo们的通常说法是，“我们别无选择”。

关键词：管理者员工福利年度报告工作热情 CEo 备忘录公司责任感

Affinity-induced covalent protein-protein ligation via the SpyCatcherSpyTag interaction

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Green Carbon 2023年第1期1卷 33-42页

作者： Jacob o.Fierer omar E.Tovar-Herrera Jonathan Y.Weinstein Amaranta Kahn Sarah Moraïs Itzhak Mizrahi edward A.Bayer Department of Biomolecular Sciences the Weizmann Institute of ScienceRehovot 7610001Israel Department of Life Sciences Ben-Gurion University of the NegevBeer Sheva 84105Israel National Institute of Biotechnology in the Negev Ben-Gurion University of the NegevBe’er Sheva 84105Israel The Goldman Sonnenfeldt School of Sustainability and Climate Change Ben-Gurion University of the NegevBeer Sheva 84105Israel

Production of economically viable bioethanol is potentially an environmentally and financially worthwhile *** major source for fermentable sugars is ***,lignocellulosic biomass is difficult to degrade,owing to its inherent structural *** are complexes of cellulases and associated polysaccharide-degrading enzymes bound to a protein scaffold that can efficiently degrade *** of the enzyme subunits into the complex depends on intermodular cohesin-dockerin interactions,which are robust but nonetheless *** modular architecture of these complexes can be used to assemble artificial designer cellulosomes for advanced nanotechnological *** that promote lignocellulose degradation involve high temperatures and acidic or alkaline conditions that could dismember designer cellulosomes,thus requiring separation of reaction steps,thereby increasing overall process *** overcome these challenges,we developed a means of covalently locking cohesin-dockerin interactions by integrating the chemistry of SpyCatcher-SpyTag approach to target and secure the *** resultant cohesin-conjugated dockerin complex was resistant to high temperatures,SDS,and urea while high affinity and specificity of the interacting modular components were *** this approach,a covalently locked,bivalent designer cellulosome complex was produced and demonstrated to be enzymatically active on cellulosic *** combination of affinity systems with SpyCatcher-SpyTag chemistry may prove of general use for improving other types of protein ligation systems and creating unconventional,biologically active,covalently locked,affinity-based molecular architectures.

关键词： Synthetic Biology Protein Ligation Cellulosomes Cohesin Dockerin Protein Engineering Cellulose Degradation

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空间单粒子效应

Compact prototype microfabricated gas chromatographic analyzer for autonomous determinations of VoC mixtures at typical workplace concentrations

作者： (美)edward Petersen

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Microsystems & Nanoengineering 2018年第1期4卷 390-399页

作者： Junqi Wang Jonathan Bryant-Genevier Nicolas Nuñovero Chengyi Zhang Bruce Kraay Changhua Zhan Kee Scholten Robert Nidetz Sanketh Buggaveeti edward T.Zellers Department of Chemistry University of MichiganAnn ArborMI 48109USA Department of Environmental Health Sciences University of MichiganAnn ArborMI 48109USA Applied Physics Program University of MichiganAnn ArborMI 48109USA Department of Mechanical Engineering University of MichiganAnn ArborMI 48109USA Center for Wireless Integrated MicroSensing and Systems University of MichiganAnn ArborMI 48109USA

This report concerns a benchtop prototype instrument containing a gas chromatographic microanalytical system(μGC)designed for the selective determination of multiple airborne volatile organic compounds(VoCs)at concentrations in the vicinity of recommended occupational exposure *** core microsystem consists of a set of discrete Si-microfabricated devices:a dualcavity,adsorbent-packed micro-preconcentrator-focuser(μPCF)chip that quantitatively captures and thermally desorbs/injects VoCs with vapor pressures between~0.03 and 13 kPa;tandem micro-column(μcolumn)chips with cross-linked PDMS wall-coated stationary phases capable of temperature-programmed separations;and an integrated array of fiveμchemiresistors(μCR)coated with different thiolate-monolayer protected gold nanoparticle(MPN)interface films that quantifies and further differentiates among the analytes by virtue of the response patterns *** key components include a pre-trap for low-volatility interferences,a split-flow injection valve,and an onboard He carrier–gas *** assembled unit measures 19×30×14 cm,weighs~3.5 kg,operates on AC power,and is laptop/LabVIEW ***-and system-level tests of performance demonstrated injection bandwidths o1 s,aμcolumn capacity of≥8μg injected mass,linear calibration curves,no humidity effects,excellent medium-term(that is,1 week)reproducibility,autonomous operation for 8 h,detection limits below Threshold Limit Values(TLV)for 10 mL air samples collected in 1 min,and response patterns that enhanced vapor *** determination of a 17-VoC mixture in the presence of seven interferences was performed in 4 *** augur well for adapting the microsystem to an all-MEMS wearableμGC currently under parallel development.

关键词： gas chromatography micro-GC microsystem mixtures sensor array VoCs

Response of Nitrous oxide Flux to Addition of Anecic Earthworms to an Agricultural Field

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open Journal of Soil Science 2013年第2期3卷 100-106页

作者： José A. Amador edward J. Avizinis Laboratory of Soil Ecology and Microbiology 024 Coastal Institute University of Rhode Island Kingston USA.

The burrowing and feeding activities of earthworms may have a strong effect on the flux of N2o from agricultural soils. As such, shifts to agricultural management practices that increase the number of earthworms require an understanding of the role of earthworms in N2o dynamics. We conducted a field experiment to examine the effects of addition of anecic earthworms (Lumbricus terrestris) on N2o flux in a field previously planted with corn (Zea mays) in southern Rhode Island, USA. Plots were amended with (15NH4)2So4 and either 0 (CTL) or 48 L. terrestris m-2 (EW). The flux of N2o, 15N2o and 15N2 was measured over 28 days between october and November 2008. The EW treatment had a significantly higher flux of N2o and 15N2o 1 - 3 days after 15NH4 addition. No treatment effects were observed on 15N2 flux. The addition of earthworms significantly increased (Day 1) and decreased (Day 12) the mole fraction of N2o relative to the CTL. our results suggest that anecic earthworm additions can increase N2o flux from inorganic fertilizer N amendments, but the effects appear to short-lived.

关键词： Lumbricus terrestris Anecic Earthworms Fertilizer Nitrogen Nitrous oxide Flux Denitrification

Rapid, simple, low-cost smartphone-based fluorescence detection of Escherichia coli

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International Journal of Agricultural and Biological Engineering 2021年第3期14卷 189-193页

作者： Dante Rojas-Barboza edward Park Rolfe Sassenfeld Jeremy Winder Geoffrey B.Smith Delia Valles-Rosalles Efren Delgado Young Ho Park Family and Consumer Science Department New Mexico State UniversityLas CrucesNM 883003USA Biology Department University of PennsylvaniaPhiladelphiaPA 19104USA Electronics&Computer Engineering Technology New Mexico State UniversityLas CrucesNM 88003USA Biology Department New Mexico State UniversityLas CrucesNM 88003USA Industrial Engineering Department New Mexico State UniversityLas CrucesNM 88003USA Mechanical Engineering Department New Mexico State UniversityLas CrucesNM 88003USA

Food and waterborne diseases pose considerable public health threats even in highly industrialized parts of the *** of these pathogens in food can be Escherichia coli o157:H7,Salmonella sp.,and Listeria ***,reliable detection of pathogens mitigates serious health problems and economic losses due to outbreaks and robust tests safeguard the food *** this study,a smartphone-based apparatus was employed to demonstrate quantitative detection of *** validate the applicability of the present smartphone-based fluorescence device,RNA was extracted from the *** K-12 strain and amplified using two different primers(dnaK and rpoA)via quantitative polymerase chain reaction(qPCR).Serial dilutions of RNA from 10 to 0.0001 ng/μL were prepared at the start of the PCR amplification and the PCR products were detected by CYBR Green1-based *** a proof-of-concept test for the smartphone system,samples from these PCR products were then *** detection system employed a novel algorithm to analyze fluorescence signals and read changes in *** DNA *** correlations between the fluorescence percentage and DNA concentrations were R=0.945 for the dnaK primer and R=0.893 for the rpoA primer,*** this new fluorescent analysis technique resulted in comparable accuracy to the real-time PCR fluorescent signal *** key innovation of this approach was to combine efficient image processing encoded into a smartphone application with a low-cost 3-D printed device that allowed quantification of bacterial nucleic acid.

关键词： smartphone fluorescence E.coli low-cost 3D-print

Catalytic Hydrothermal Liquefaction of Water Hyacinth Using Fe₃o₄/Nio Nanocomposite: optimization of Reaction Conditions by Response Surface Methodology

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Journal of Sustainable Bioenergy Systems 2023年第3期13卷 73-98页

作者： Godwin Aturagaba Dan Egesa edward Mubiru Emmanuel Tebandeke Department of Chemistry College of Natural Sciences School of Physical Sciences Makerere University Kampala Uganda

This research aimed at optimizing the reaction conditions for the catalytic hydrothermal liquefaction (HTL) of water hyacinth using iron oxide/nickel oxide nanocomposite as catalysts. The iron oxide/nickel oxide nanocomposite was synthesized by the co-precipitation method and used in the hydrothermal liquefaction of water hyacinth. The composition and structural morphology of the synthesized catalysts were determined using X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic absorption spectroscopy (AAS). The particle size distribution of the catalyst nanoparticles was determined by the Image J software. Three reaction parameters were optimized using the response surface methodology (RSM). These were: temperature, residence time, and catalyst dosage. A maximum bio-oil yield of 59.4 wt% was obtained using iron oxide/nickel oxide nanocomposite compared to 50.7 wt% obtained in absence of the catalyst. The maximum bio-oil yield was obtained at a temperature of 320°C, 1.5 g of catalyst dosage, and 60 min of residence time. The composition of bio-oil was analyzed using gas chromatography-mass spectroscopy (GC-MS) and elemental analysis. The GC-MS results showed an increase of hydrocarbons from 58.3% for uncatalyzed hydrothermal liquefaction to 88.66% using iron oxide/nickel oxide nanocomposite. Elemental analysis results revealed an increase in the hydrogen and carbon content and a reduction in the Nitrogen, oxygen, and Sulphur content of the bio-oil during catalytic HTL compared to HTL in absence of catalyst nanoparticles. The high heating value increased from 33.5 MJ/Kg for uncatalyzed hydrothermal liquefaction to 38.6 MJ/Kg during the catalytic HTL. The catalyst nanoparticles were recovered from the solid residue by sonication and magnetic separation and recycled. The recycled catalyst nanoparticles were still efficient as hydrothermal liquefaction (HTL) catalysts and were recycled four times. The application o

关键词： Catalytic Hydrothermal Liquefaction Water Hyacinth Bio-oil Central Com-posite Design Response Surface Methodology optimization

The Industrial Production of Water Dedicated to Absorption of Gases

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Journal of Water Resource and Protection 2021年第8期13卷 632-653页

作者： Ihar Yelkin edward Reszke Grzegorz Binkiewicz Grzegorz Schroeder Research and Development Department Plasma Investment Ltd. Wroclaw Poland Ertec-Poland Wroclaw Poland Faculty of Chemistry Adam Mickiewicz University in Poznan Poznan Poland

The paper presents a flow plasma reactor permitting modification of the properties of water/aqueous solutions by stochastic resonance amplification of vibrations of selected chemical species in water with electromagnetic noise generated during a plasma discharge. The main parameters characterizing the quality for super-pure water, tap water and water from the intake in Besko (Poland) before and after the process in the plasma reactor were presented for comparison. In addition, the 17o NMR (the full width at half maximum) and electrospray ionization mass spectrometry (ESI MS) methods were used to determine differences in physicochemical parameters between the untreated and plasma-treated water. It has been established that the water subjected to plasma treatment shows much different gas absorption properties than the untreated water samples, as a function of temperature and pressure, in this paper we report exemplary data for Co2, oxygen and acetylene. The improved gas absorption properties of the plasma-treated water make it attractive for the use in industrial processes. It is worth pointing to a great capacity of the new reactor (4000 l/h), and low energy consumption (20 MJ/h) for the treatment of the above mentioned volume flow rate of water.

关键词： Flow-Through Plasma Reactor for Water Treatment Modification of the Properties of Water/Aqueous Solutions Industrial Production of Water Physicochemical Parameters of Water Solution