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Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

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Journal of Forestry Research 2021年第4期32卷 1587-1598页

作者： Letícia Vaz Molinari denys Matheus Santana Costa souza Maria Lopes martins Avelar Sérgio Bruno fernandes Douglas Santos Gonçalves Júlio Cézar Tannure Faria Dulcineia de Carvalho Gilvano Ebling Brondani department of Forestry Sciences Federal University of LavrasLavrasMinas GeraisBrazil

Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality ***,most of these species have difficulties surviving in vitro *** chlorine and sealing systems are often used to reduce contamination and increase gas *** aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of *** × *** clones(C1 and C2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication *** sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation *** luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious *** chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro *** chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication *** were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an *** increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.

关键词： Asepsis Cloning Gas exchange In vitro propagation

Extrahepatic right hepatic duct diverticulum: a rare entity

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Hepatobiliary & Pancreatic Diseases International 2010年第2期9卷 213-215页

作者： eduardo SM fernandes Raquel L Bernardo Moacir M fernandes Rogério MC Araújo Renato Sebbe Asterio Monte JoséF Coelho Antonio AP souza Joaquim Ribeiro Filho department of General Surgery Section of Hepatobiliary Surgery and TransplantationRio de Janeiro Federal University department of Digestive Surgery and Gastroenterology Pio XII General Hospital

BACKGROUND:Douglas described choledochal cysts in 1852 and Todani proposed an anatomy-based classification in 1977. The classification is the most extensively used,but does not include some rare *** present a case of hepatic duct diverticulum,one of the variations,and discuss its diagnosis and treatment. METHODS:A 57-year-old woman presented with upper abdominal pain and discomfort associated with nausea and *** was finally diagnosed with cholelithiasis and right hepatic duct diverticulum. RESULT:The patient underwent resection of the hepatic duct diverticulum and cholecystectomy,and was asymptomatic 26 months after surgery. CONCLUSIONS:Hepatic duct diverticulum is a rare form of choledochal cyst,not included in Todani’s classification. Todani’s classification including this and other uncommon variations of choledochal cysts must be *** best diagnostic imaging methods and treatment for choledochal cysts must be defined.

关键词： choledochal cysts hepatic duct diverticulum Todani's classification intrahepatic diverticulum

Effects of the use of bioceramic wraps in patients with lower limb venous ulcers:A randomized double-blind placebo-controlled trial

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Journal of Integrative Medicine 2020年第1期18卷 26-34页

作者： Gerli E.G.Herr Fábio Goulart da Silva Francisco Jose Cidral-Filho Fabricia Petronilho Lucinéia Gainski Danielski Mariana Pereira de souza Goldim Afonso Shiguemi Inoue Salgado Franciane Bobinski Daniel fernandes martins Eliane R.Winkelmann Integral Health Care Regional University of the Northwest of the State of Rio Grande do Sul(UNIJUí)and University of Cruz Alta(UNICRUZ)Ijuí98700-000RSBrazil Experimental Neuroscience Laboratory(LaNEx) University of Southern Santa CatarinaPalhoca 88137-270SCBrazil Laboratory of Neurobiology of Inflammatory and Metabolic Processes University of Southern Santa CatarinaTubarao 88704-900SCBrazil Integrative Physical Therapy Residency Philadelphia University CenterLondrina 86020-000PRBrazil Laboratory of Exercise Physiopathology Department of Life Sciences(DCVida)Regional University of the Northwest of the State of Rio Grande do Sul(UNIJUí)Ijuí98700-000RSBrazil

Background:Venous ulcer represents the most advanced stage of chronic venous *** is an important public health problem and has a significant impact on patients’quality of life due to chronic pain,inability to work,need for hospitalization and frequent outpatient ***:We investigated the treatment benefits of far-infrared ceramic(cFIR),in a 90-day study of lower limb venous ulcers and looked at ulcer healing scores,quality of life,serum bio-markers of oxidative stress and antioxidant defense ***,setting,participants and interventions:This is a randomized double-blind placebo-controlled study conducted in the Vascular Surgery Service of a hospital located in the northwest region of the State of Rio Grande do Sul,*** included patients with lower limb venous ulcers who were randomized to use either a bioceramics wrap or a placebo wrap for 90 *** outcome measures:The following evaluations were conducted at baseline and after 15,30,60 and90 days:ulcer healing score,quality of life,and serum markers of oxidative stress and antioxidant enzyme ***:Patients(n=24)with lower limb venous ulcers were randomized into two treatment *** decreased the ulcer size on day 30(P=0.042)and 90(P=0.034)and the total ulcer healing scale scores on day 30(P=0.049)and 90(P=0.02)of the treatment,when compared to ***,cFIR improved tissue type(epithelial tissue)on day 60(P=0.022)when compared to baseline ***:cFIR clinically improved ulcer healing in patients with lower limb venous *** registration:RBR-8c7xzn on ReBEC.

关键词： Far-infrared radiation Quality of life Oxidative stress Wound healing Randomized controlled trial Bioceramic wrap

Comprehensive analysis of HFE gene in hereditary hemochromatosis and in diseases associated with acquired iron overload

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World Journal of Hepatology 2019年第2期11卷 186-198页

作者： Wagner Narciso de Campos Juliana Doblas Massaro eduardo Luiz Rachid Can?ado Cláudia Emília Vieira Wiezel Aguinaldo Luiz Sim?es Andreza Correa Teixeira Fernanda fernandes de souza Celso Teixeira Mendes-Junior Ana de Lourdes Candolo Martinelli eduardo Ant?nio Donadi Division of Clinical Immunology Department of MedicineRibeir?o Preto Medical SchoolUniversity of S?o Paulo department of Gastroenterology Clinical Gastroenterology and Clinical Hepatology of Clinical HospitalUniversity of S?o Paulo School of Medicine department of Genetics Ribeir?o Preto Medical SchoolUniversity of S?o Paulo Division of Gastroenterology Department of MedicineRibeir?o Preto Medical SchoolUniversity of S?o Paulo departamento de Química Laboratório de Pesquisas Forenses e Gen?micasFaculdade de FilosofiaCiências e Letras de Ribeir?o PretoUniversidade de S?o Paulo

BACKGROUND Patients with hepatitis C virus(HCV) and hepatocellular carcinoma(HCC) may or not develop iron overload(IO),which is associated with worst prognosis,because can cause serious damage to *** gene controls the iron uptake from gut,particularly in patients with hereditary hemochromatosis(HH).AIM To identify associations between HFE coding region in patients exhibiting hereditary hemochromatosis and in diseases associated with acquired *** We sequenced exons 2 to 5 and boundary introns of HFE gene,evaluating all polymorphic sites in patients presenting hereditary(hemochromatosis) or acquired iron overload HCV and HCC) and in healthy controls,using Sanger *** also determined the ensemble of extended haplotype in healthy control individuals,including several major histocompatibility complex loci,using sequence specific *** reconstruction was performed using the Arlequin and Phase softwares,and linkage disequilibrium(LD) between histocompatibility loci and HFE gene was performed using the Haploview *** The HFE*003 allele was overrepresented(f = 71%) and HFE*001 allele was underrepresented(f = 14%) in HH patients compared to all groups.A strong linkage disequilibrium was observed among the H63 D-G,IVS2(+4)-C and C282 YG gene variants,particularly in HH;however,the mutation IVS2(+4)T>C was not directly associated with HH *** HFE*001/HFE*002 genotype conferred susceptibility to HCC in HCV patients exhibiting IO(P = 0.02,OR =14.14).Although HFE is telomeric to other histocompatibility genes,the H63 DG/IVS2(+4)-C(P ≤ 0.00001/P ≤ 0.0057) combination was in LD with HLA-B*44 allele group in healthy *** LD was observed between HFE alleles and other major histocompatibility *** A differential HFE association was observed for HH and for diseases associated with acquired IO(HCV,HCC).Since HFE is very distant from other histocompatibility loci,only weak associations were observed with these a

关键词： HFE gene Hepatocellular carcinoma Hepatitis C Hemochromatosis hereditary Alleles Haplotypes

Landscape Structural Indicators as a Tool to Assess Land Use Changes in Planning for Sub-Basin Sustainability (Southeastern Brazil)

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Journal of Water Resource and Protection 2016年第4期8卷 482-492页

作者： Angela Terumi Fushita José eduardo dos Santos Imyra Maíra martins de souza Eduarda Romanini Rômulo Theodoro Costa Analysis and Environmental Planning Laboratory Department of Hydrobiology Federal University of S&#227o Carlos (UFSCar) S&#227o Carlos Brazil Pos-Graduate Programme in Ecology and Natural Resources Federal Universityof S&#227o Carlos (UFSCar) S&#227o Carlos Brazil

Land use is shaped by the interactions between nature and society, and these interactions can inhibit environmental sustainability and deplete the natural capital that provides the ecosystem services upon which humans depend. Urbanity Index and Landscape Vulnerability Indicator have been proposed to improve the impact interpretability of land use changes on sub-basin sustainability for local scenarios of biodiversity conservation. A time series of LandSat 5 Thematic Mapper remote sensing data from São Carlos municipality, Southeastern Brazil, for the years 1989, 2004 and 2014 revealed that land use changes in the sub-basins do not take place in a progressive and gradual way. Over the 25-year period, the main trends showed the loss and increase of forest cover so that it has remained quantitatively similar over time due to reduced agricultural land use. The aggregation of both indicators enabled the identification of greater naturalness and lower vulnerability, as well as lower naturalness and higher vulnerability under local sub-basin conditions, pointing the need for different strategies for sub-basin biodiversity conservation and sustainability. These preliminary scenarios provide a way to communicate problems of environmental sustainability at different landscape scales to the scientific community as well as to planners, policy makers and the broader public.

关键词： Land Use Quantifiable Indicator Urbanity Index Landscape Vulnerability Indicator

Mathematical Modeling and determination of Effective Diffusivity of Mesquite during Convective Drying

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American Journal of Plant Sciences 2016年第6期7卷 814-823页

作者： Rennan Pereira de Gusmão Thaisa Abrantes souza Gusmão Mário eduardo Rangel Moreira Cavalcanti-Mata Maria Elita martins Duarte Federal University of Campina Grande Campina Grande Brazil

The mesquite pods have a high nutritional value, being a rich source of sugars, proteins, minerals and fibers that can be used as a raw material for the development of a number of food products and technological innovations. The objective of this work was to study the drying kinetics of mesquite pods in a thin layer, 20 mm length and determine the effective diffusion coefficient by adjusting mathematical models that were based on heat transfer and mass fundamentals. For the experiments, we used mature mesquite, with 20% water content (b.u). Drying experiments were performed using temperatures of 50°C, 60°C, 70°C and 80°C with drying air speed of 2 m•s-1. It was used a convective dryer with fixed bed and upflow air. Mathematical models of Fick, Page, Cavalcanti-Mata, Two Exponential Terms and Henderson & Pabis were used to adjust the experimental data. To calculate the effective diffusivity, flat plate geometry with sample thickness of 6.5 mm was used. Based on the results obtained, it was concluded that the loss of water from mesquite occurred during the drying period in decreasing rate;and with increasing drying temperature, total process time was reduced. The diffusion coefficient increased by increasing drying temperature. Mathematical models including theoretical (Fick), semi-theoretical (Page and Cavalcanti-Mata) and empirical (Two Exponential Terms) models satisfactorily explain the experimental data of drying mesquite.

关键词： Diffusion Coefficient Prosopis juliflora Subproduct

The Fertilizer-Effect on Al, Ba, Fe, Mn and Ni Released in a Watershed with Influence of Sugar Cane Crops in the São Paulo State, Brazil

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Journal of Water Resource and Protection 2019年第5期11卷 638-650页

作者： Eder Paulo Spatti Jú nior Fabiano Tomazini da Conceiç o Amauri Antonio Menegá rio Alexandre martins fernandes Diego de souza Sardinha Instituto de Geociê ncias e Ciê ncias Exatas UNESP-Universidade Estadual Paulista S&#227 o Paulo Brasil Centro de Estudos Ambientais UNESP-Universidade Estadual Paulista S&#227 o Paulo Brasil UNIFAL-Universidade Federal de Alfenas Po&#231 os de Caldas Brasil

S?o Paulo State is the main sugar cane producer and these agricultural activities are carried out in predominantly sandy soils, which require large amounts of phosphate fertilizers and amendments. This work evaluated the fertilizer-effect on the Al, Ba, Fe, Mn and Ni released in a watershed with influence of sugar cane crops in the S?o Paulo State, Brazil, namely the Monjolo Grande Stream basin. Five surface water sampling campaigns were carried out at the mouth of Monjolo Grande Stream in February, April, June, September and November 2010, characterizing the following parameters: discharge, pH, temperature, electric conductivity, dissolved oxygen and total and dissolved concentrations of Al, Ba, Fe, Mn and Ni. Approximately 99% of Al and Fe are transported annually in association with suspended sediments carried to the Monjolo Grande Stream by sheet erosion. The results also demonstrated that the increasing Al, Ba, Fe and Mn concentrations dissolved in the waters of the Monjolo Grande Stream basin in the wet season are associated to phosphate fertilizers and amendments that are used extensively in agrichemical activities. However, with the current application rates, there has been no increase in the dissolved concentrations of these metals at levels that could pose risks to human health.

关键词： Sugar Cane Crops Phosphate Fertilizers and Amendments Total and Dissolved Metal Environmental Management

Cardiac Perforation by Firearm:Case Report

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World Journal of Cardiovascular Surgery 2018年第10期8卷 189-195页

作者： Gerez fernandes martins Claudio Assunpcao Barbara Jessen Gerez martins Joao de deus e Brito Cardiac Surgery department Institute of Cardiology State Aloysio de CastroRio de JaneiroBrazil Cardiology department Institute of Cardiology State Aloysio de CastroRio de JaneiroBrazil Surgery department Souza Marques UniversityRio de JaneiroBrazil

Heart trauma caused by a firearm projectile is the most fatal of cardiac injuries due to the great potential for death. The increase in the number of cases in recent years is observed, due to the greater urban violence. We report the case of a patient, a victim of firearm perforation, who suffered a cardiac transfixing lesion, with clinical presentation and with an unusual cardiac injury, operated at our service with success.

关键词： Heart Trauma Firearm Perforation Cardiac Lesion Urban Violence Cardiac Surgery

Agriculture and Forest Transition: Understanding of Land Use Change in a Cultural Landscape

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Open Journal of Applied Sciences 2015年第12期5卷 797-807页

作者： José eduardo dos Santos Angela Terumi Fushita Imyra Maíra martins de souza Lia Martucci Amorim Valéria Aparecida David Andrade department of Hydrobiology Federal University of S&atilde o Carlos S&atilde o Carlos Brazil Postgraduate Program in Ecology and Natural Resources Federal Universityof S&atilde o Carlos S&atilde o Carlos Brazil

Land use trajectory analysis provides both the time and kind of land cover changes to monitor land use dynamics. Land cover change trajectories for three different dates (1965, 1989 and 2014) extracted from satellite images by vectorization of the thematic information were studied This study was carried out on a cultural landscape (S?o Carlos municipality, SP, Brazil) with three major land use-cover classes (forest, agriculture and other uses). The results showed the spatio temporal variability of landscape pattern and forest and agriculture change trajectories. Analysis based on these landscape trajectories demonstrates that agriculture and forest cover changes have been caused by human activities. The results reflect the conflicting interactions between environmental and human systems in the study area. A key question is that the pressure exerted on forest land use-cover depends on the incentives that move society from a conflicting relation with a municipal territory. A more sustainable landscape transition and trajectory for S?o Carlos municipality are extremely dependent on the regulatory role of the government through strategies related to the implementation of specific categories of legally protected areas (Legal Reserves and Areas of Permanent Preservation).

关键词： Land Use-Cover Change Trajectories Landscape Sustainability Legally Protected Areas Remote Sensing