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Multi-envir.nment BSA-seq using lar.e F3 populations is able to achieve r.liable QTL mapping with high power.and r.solution: An exper.mental demonstr.tion in r.ce

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The Cr.p Jour.al 2024年第2期12卷 549-557页

作者： Yan Zheng ei ei Khine Khin Mar.Thi ei ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weir.n Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian Pr.vincial Key Labor.tor. of Cr.p Br.eding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Labor.tor. of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Depar.ment of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Depar.ment of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segr.gant analysis by deep sequencing(BSA-seq) is a widely used method for.mapping QTL(quantitative tr.it loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However. the ability of BSA-seq to detect QTL is often limited by inappr.pr.ate exper.mental designs, as evidenced by numer.us pr.ctical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Never.heless, theor.tical studies have shown that using a lar.e population with an appr.pr.ate pool size can significantly enhance the power.and r.solution of QTL detection in BSA-seq, with F_(3)populations offer.ng notable advantages over.F2populations. To pr.vide an exper.mental demonstr.tion, we tested the power.of BSA-seq to identify QTL contr.lling days fr.m sowing to heading(DTH) in a 7200-plant r.ce F_(3)population in two envir.nments, with a pool size of appr.ximately 500. Each exper.ment identified 34 QTL, an or.er.of magnitude gr.ater.than r.por.ed in most BSA-seq exper.ments, of which 23 wer. detected in both exper.ments, with 17 of these located near.1 pr.viously r.por.ed QTL and eight cloned genes known to contr.l DTH in r.ce. These r.sults indicate that QTL mapping by BSA-seq in lar.e F_(3)populations and multi-envir.nment exper.ments can achieve high power. r.solution, and r.liability.

关键词： BSA-seq QTL mapping Lar.e F3 population Multi-envir.nment exper.ment Cr.ss-validation

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How Can Pr.ctitioner. Suppor. Citizen Volunteer. in Disaster.r.sk r.duction? Insight fr.m “Good and r.ady” in Aotear.a New Zealand

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Inter.ational Jour.al of Disaster.r.sk Science 2024年第3期15卷 374-387页

作者： Lo?c Le Dé Steve r.noh ei Mon Thinn Kyu Br.gitte r.ve School of Public Health and Inter.isciplinar. Studies Auckland University of Technology Pacific Health r.sear.h Centr. Auckland University of Technology New Zealand r.d Cr.ss

Global and national policy fr.mewor.s emphasize the impor.ance of people's par.icipation and volunteer.' r.le in disaster.r.sk r.duction. While r.sear.h has extensively focused on volunteer. in disaster.r.sponse and r.cover., less attention has been paid on how or.anizations involved in disaster.r.sk management can suppor. volunteer. in leading and coor.inating communitybased disaster.r.sk r.duction. In 2019, the New Zealand r.d Cr.ss piloted the Good and r.ady initiative in Auckland,Aotear.a New Zealand, with the objective to empower.local people in r.silience building with a focus on volunteer. and community par.icipation. This r.sear.h examined the positive and negative outcomes of Good and r.ady and investigated volunteer.' exper.ences in the disaster.r.silience initiative. It involved the codesign of a questionnair.-based sur.ey using par.icipator. methods with Good and r.ady volunteer., the dissemination of the sur.ey to gather.volunteer.' viewpoints, and a focus gr.up discussion with par.icipator. activities with r.d Cr.ss volunteer.. The findings highlight that a key challenge lies in finding a balance between a pr.gr.m that pr.vides flexibility to addr.ss contextual issues and foster. communities' owner.hip, ver.us a pr.scr.ptive and standar.ized appr.ach that leaves little r.om for.cr.ativity and self-initiative. It pinpoints that suppor.ing volunteer. with technical tr.ining is cr.tical but that soft skills tr.ining such as coor.inating, communicating,or.facilitating activities at the local level ar. needed. It concludes that the sustainability of Good and r.ady r.quir.s under.tanding and meeting volunteer.' motivations and expectations and that enhancing par.ner.hips with local emer.ency management agencies would str.ngthen the pr.gr.m.

关键词： Community-based appr.ach Disaster.r.sk r.duction New Zealand Par.icipation Volunteer.

Machine lear.ing and bioinfor.atics to identify biomar.er. in r.sponse to Bur.holder.a pseudomallei infection in mice

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BIOCELL 2024年第4期48卷 613-621页

作者： YAO FANG Fei XIA FeiFei TIAN L ei QU FANG YANG JUAN FANG ZHENHONG HU HAICHAO LIU Depar.ment of r.spir.tor. and Cr.tical Car. Medicine General Hospital of Center Theater of PLAWuhan430070China School of Medicine Wuhan University of Science and TechnologyWuhan430065China School of Life Science and Engineer.ng Southwest Jiaotong UniversityChengdu611756China

Objective:In the r.alm of Class I pathogens,Bur.holder.a pseudomallei(BP)stands out for.its pr.pensity to induce sever. pathogenicity.Investigating the intr.cate inter.ctions between BP and host cells is imper.tive for.compr.hending the dynamics of BP infection and discer.ing biomar.er. indicative of the host cell r.sponse pr.cess.Methods:mr.A extr.ction fr.m BP-infected mouse macr.phages constituted the initial step of our.study.Employing gene expr.ssion ar.ays,the extr.cted r.A under.ent conver.ion into digital signals.The per.entile shift method facilitated data pr.cessing,with the identiﬁcation of genes manifesting signiﬁcant differ.nces accomplished thr.ugh the application of the t-test.Subsequently,a compr.hensive analysis involving Gene Ontology enr.chment and Kyoto Encyclopedia of Genes and Genomes pathway was conducted on the differ.ntially expr.ssed genes(DEGs).Lever.ging the ESTIMATE algor.thm,gene signatur.s wer. utilized to compute r.sk scor.s for.gene expr.ssion data.Suppor. vector.machine analysis and gene enr.chment scor.s wer. instr.mental in establishing cor.elations between biomar.er. and macr.phages,followed by an evaluation of the pr.dictive power.of the identiﬁed biomar.er..r.sults:The functional and pathway associations of the DEGs pr.dominantly center.d ar.und G pr.tein-coupled r.ceptor..A notewor.hy positive cor.elation emer.ed between the blue module,consisting of 416 genes,and the Str.maScor..FZD4,identiﬁed thr.ugh suppor. vector.machine analysis among inter.ecting genes,indicated a r.bust inter.ction with macr.phages,suggesting its potential as a r.bust biomar.er.FZD4 exhibited commendable pr.dictive efﬁcacy,with BP infection inducing its expr.ssion in both macr.phages and mouse lung tissue.Wester. blotting in macr.phages conﬁr.ed a signiﬁcant upr.gulation of FZD4 expr.ssion fr.m 0.5 to 24 h post-infection.In mouse lung tissue,FZD4 manifested higher.expr.ssion in the cytoplasm of pulmonar. epithelial cells in BP-infected lungs than in the contr.l gr.up.Conclusion:Theseﬁndings under.cor

关键词： Bur.holder.a pseudomallei Micr.ar.ay assay Machine lear.ing Bioinfor.atics FZD4

Matr.x-assisted laser.desor.tion/ionization time-of-flight mass spectr.metr. tr.ces the geogr.phical sour.e of Biomphalar.a pfeiffer. and Bulinus for.kali,involved in schistosomiasis tr.nsmission

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Infectious Diseases of Pover.y 2024年第1期13卷 98-99页

作者： Papa Mouhamadou Gaye ei Hadj Ibr.hima Ndiaye Souleymane Doucour. Doudou Sow Mapenda Gaye Ndiaw Goumballa Car.le Cassagne Cor.lie L'Ollivier.Oleg Medianikov Cheikh Sokhna Stephane r.nque Aix-Mar.eille Univer.ity IRD AP-HM SSA VITROME Marseille France VITr.ME International IRD-UCAD Campus Dakar Senegal Hospital-Univer.ity Institut (IHU) Mediter.anée Infection Marseille France Depar.ement of Animal Biology Faculty of Sciences and Techniques UCAD Dakar Senegal Depar.ment of Par.sitology-Mycology UFR Sciences de la Santé Université Gaston Berger Saint-Louis Senegal

Backgr.und Fr.shwater.snails of the gener. Bulinus spp.,Biomphalar.a spp.,and Oncomelania spp.ar. the main inter.ediate hosts of human and animal schistosomiasis.Identification of these snails has long been based on mor.phological and/or.genomic cr.ter.a,which have their.limitations.These limitations include a lack of pr.cision for.the mor.hological tool and cost and time for.the DNA-based appr.ach.r.cently,Matr.x-Assisted Laser.Desor.-tion/lonization Time-Of-Flight(MALDI-TOF)mass spectr.metr.,a new tool used which is r.utinely in clinical micr.bi-ology,has emer.ed in the field of malacology for.the identification of fr.shwater.snails.This study aimed to evaluate the ability of MALDI-TOF MS to identify Biomphalar.a pfeiffer. and Bulinus for.kali snail populations accor.ing to their.geogr.phicalor.gin.Methods This study was conducted on 101 Bi.pfeiffer. and 81 Bu.for.kali snails collected in thr.e distinct geo-gr.phical ar.as of Senegal(the Nor.h-East,South-East and centr.l par. of the countr.),and supplemented with wild and labor.tor. str.ins.Specimens which had pr.viously been mor.hologically descr.bed wer. identified by MALDl-TOF MS[identification log scor. values(LSV)≥1.7],after.an initial blind test using the pr.-existing database.After.DNA-based identification,new r.fer.nce spectr. of Bi.pfeifer.(n=10)and Bu.for.kali(n=5)fr.m the geogr.phical ar.as wer. added to the MALDI-TOF spectr.l database.The final blind test against this updated database was per.for.ed to assess identification at the geogr.phic sour.e level.r.sults MALDI-TOF MS cor.ectly identified 92.1%of 101 Bi.pfeiffer. snails and 98.8%of 81 Bu.for.kali snails.At the final blind test,88%of 166 specimens wer. cor.ectly identified accor.ing to both their.species and sampling site,with LSVs r.nging fr.m 1.74 to 2.70.The geogr.phical sour.e was adequately identified in 90.1%of 91 Bi.pfeiffer. and 85.3%of 75 Bu.for.kalii samples.Conclusions Our.findings demonstr.te that MALDI-TOF MS can identify and differ.ntiate snail populations accor.ing to geogr.phic

关键词： Matr.x-assisted laser.desor.tion/ionization time-of-flight mass spectr.metr. Snail Inter.ediate host Biomphalar.a pfeiffer. Bulinus for.kali Schistosomiasis Senegal

对比与融合巴塞罗那PJ.Lobster.学精品店

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室内设计与装修 2024年第5期 82-85页

作者： ei Depar.amento ei Depar.amento 西班牙

El Depar.amento设计工作室以其独到的创意,为全渠道眼镜零售品牌PJ.Lobster.巴塞罗那Bor.区的门店注入了新的生机。该空间以精致的单色内饰引人注目,通过巧妙的布局和色彩运用,营造出动态且富有吸引力的实体零售环境。这不仅仅是一个... 详细信息

El Depar.amento设计工作室以其独到的创意,为全渠道眼镜零售品牌PJ.Lobster.巴塞罗那Bor.区的门店注入了新的生机。该空间以精致的单色内饰引人注目,通过巧妙的布局和色彩运用,营造出动态且富有吸引力的实体零售环境。这不仅仅是一个购物空间,更是一个让顾客能够亲身体验品牌精神和设计理念的场所。同时,该店铺以其独特的设计风格,成功地从众多参赛作品中脱颖而出。

关键词：设计工作室巴塞罗那购物空间品牌精神亲身体验全渠道色彩运用设计理念

一株柠檬内生细菌Z10的分离鉴定及其抑菌活性的发酵条件优化

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微生物学通报 2024年第5期51卷 1641-1658页

作者：钱程叶景 Khadija ei Galai 张琴高梦蝶杨新宇喻艳玲唐松安徽工程大学生物与食品工程学院安徽芜湖241000

【背景】近年来,从植物内生菌的发酵产物中挖掘抑菌活性成分已成为寻找新型生物活性成分的重要途径。【目的】寻找新的具有高效抗菌活性的植物内生菌,并探究其产生抗菌活性物质的最优发酵条件和抑菌机制。【方法】利用组织分离法和划线... 详细信息

【背景】近年来,从植物内生菌的发酵产物中挖掘抑菌活性成分已成为寻找新型生物活性成分的重要途径。【目的】寻找新的具有高效抗菌活性的植物内生菌,并探究其产生抗菌活性物质的最优发酵条件和抑菌机制。【方法】利用组织分离法和划线法从柠檬皮和籽核中分离内生菌株,按照滤纸片和牛津杯法筛选对病原菌具有较强抑制作用的菌株,通过形态观察和16S r.NA基因序列分析确定活性菌株的分类地位,基于微孔板法检测活性菌株发酵液提取液(B02c)的抑菌效果,以单因素试验和响应面试验设计对发酵条件进行优化,并通过碘化丙啶/SYTO-9探针标记结合流式细胞术检测B02c对检定菌细胞膜的影响。【结果】从柠檬籽核中筛选出一株对金黄色葡萄球菌(Staphylococcus aur.us)具有显著抑制作用的菌株Z10,鉴定为芽孢杆菌(Bacillus sp.);菌株Z10的最佳发酵条件为柑橘皮渣添加量1.6 g/100 mL、pH 6.8、接种量10.0%、转速172 r.min、培养时间36 h,优化后B02c的抑菌率达到84.36%,较优化前提升16.36%;B02c对金黄色葡萄球菌的最低抑制浓度(minimum inhibitor. concentr.tion,MIC)为3.125 mg/mL,其能够破坏金黄色葡萄球菌细胞膜的完整性。【结论】柠檬内生菌Z10在优化发酵条件后获得的B02c对金黄色葡萄球菌具有良好的抑菌效果,在新型、高效的天然抑菌活性物质筛选及其工业化生产中具有一定应用前景。

关键词：分离鉴定芽孢杆菌内生菌抑菌机制发酵优化

腐蚀疲劳交替下2A12-T4航空铝合金的寿命分析研究

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机械设计与制造 2024年第1期395卷 107-112页

作者：谭娜孙世磊华磊中国民航大学航空工程学院天津300300

基于飞机服役期间经历“地面腐蚀与空中疲劳”的交替损伤模式,考虑载荷间的相互作用,研究了航空铝合金2A12-T4在预腐蚀与“腐蚀+疲劳”交替模式下疲劳寿命的退化与损伤累积规律。同级加载条件下提出利用腐蚀疲劳耦合损伤指数和迟滞载荷... 详细信息

基于飞机服役期间经历“地面腐蚀与空中疲劳”的交替损伤模式,考虑载荷间的相互作用,研究了航空铝合金2A12-T4在预腐蚀与“腐蚀+疲劳”交替模式下疲劳寿命的退化与损伤累积规律。同级加载条件下提出利用腐蚀疲劳耦合损伤指数和迟滞载荷用来描述考虑交替腐蚀疲劳损伤模式,用各级疲劳损伤间的相互影响及腐蚀损伤与疲劳损伤在服役工作期间的耦合作用来描述腐蚀、疲劳间的相互促进、加速劣化的现象。基于损伤力学和非线性累积理论,考虑载腐蚀疲劳耦合指数与迟滞载荷对传统的Miner.性累积损伤理论进行修正。建立了飞机结构材料的寿命计算模型,并将模型计算结果与试验结果进行对比验证。确定了腐蚀实验中的腐蚀耦合损伤,并利用低载锻炼效应理论得出均匀分布的迟滞载荷。计算结果表明,本文提出的寿命计算模型的结果与实验结果比较吻合。

关键词：腐蚀疲劳非线性累积腐蚀耦合损伤迟滞载荷寿命计算

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酸蚀对白云石可浮性的影响及其作用机理

东北大学学报（自然科学版） 2024年第1期45卷 93-100页

作者：郭俊印万忠杨斌朱张磊东北大学资源与土木工程学院辽宁沈阳110819 中国矿业大学化工学院江苏徐州221116 西安科技大学化学与化工学院陕西西安710054

在油酸钠体系下,通过浮选试验、浮选动力学拟合、表面粗糙度表征、比表面积分析、XPS分析、吸附性能分析和润湿性分析,研究了酸蚀对白云石可浮性的影响及其作用机理.研究结果表明:酸蚀后,白云石表面粗糙度和比表面积增大,这暴露出更多... 详细信息

在油酸钠体系下,通过浮选试验、浮选动力学拟合、表面粗糙度表征、比表面积分析、XPS分析、吸附性能分析和润湿性分析,研究了酸蚀对白云石可浮性的影响及其作用机理.研究结果表明:酸蚀后,白云石表面粗糙度和比表面积增大,这暴露出更多的活性位点,强化了与油酸钠的吸附,增强了白云石的疏水性,进而改善了白云石的浮选性能.此外,在浮选动力学拟合中,经典一级动力学模型、一级矩阵分布模型、二级动力学模型的r.均高于95%.相比于其他模型,经典一级动力学拟合效果较好.其中在油酸钠质量浓度为60 mg/L时,酸蚀后白云石经典一级动力学k最大值为1.88 min^(-1),与此同时,酸蚀前白云石经典一级动力学k=1.15 min^(-1),表明酸蚀可以提高白云石浮选速率.本研究为白云石浮选提供了新的研究方向,具有一定的理论和现实意义.

关键词：白云石粗糙度酸蚀可浮性浮选动力学

纤维增强复合材料加固混凝土桥梁结构研究进展

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建筑科学与工程学报 2024年第1期41卷 31-51页

作者：梅葵花王凤轩孙胜江长安大学公路学院陕西西安710064 山东省交通规划设计院集团有限公司山东济南250101

为总结纤维增强复合材料(Fr.)在混凝土桥梁加固中的研究成果,拓宽Fr.加固桥梁的思路,推动其在桥梁加固领域的广泛应用,阐述了Fr.加固方法的发展及其特点,系统梳理了Fr.加固方法在混凝土桥梁的研究进展。根据加固机理的不同,从抗弯加固... 详细信息

为总结纤维增强复合材料(Fr.)在混凝土桥梁加固中的研究成果,拓宽Fr.加固桥梁的思路,推动其在桥梁加固领域的广泛应用,阐述了Fr.加固方法的发展及其特点,系统梳理了Fr.加固方法在混凝土桥梁的研究进展。根据加固机理的不同,从抗弯加固、抗剪加固、抗压加固三方面加以归纳总结,介绍了几种常见的Fr.加固方式、影响因素、破坏模式及其设计理论;分析了当前研究的不足,由此提出了一些可以继续深入研究的问题。结果表明:目前研究较多的有外贴Fr.法、预应力Fr.技术、表层嵌入法、Fr.网格加固技术,其在不同的设计参数和使用条件下呈现出不同的破坏模式;外贴Fr.片材容易过早发生剥离破坏,可以通过一些锚固措施提升界面黏结性,其中HB-Fr.加固技术的锚固效果最好;预应力Fr.加固技术因具有较高的材料强度利用率而展现出良好的应用前景;后续应深入研究Fr.加固设计理论,综合考虑各参数对加固结构性能的影响,建立统一可靠且实用的计算模型;应进一步研究既有混凝土加固结构在多因素耦合作用下的长期性能和耐久性能,完善Fr.加固混凝土桥梁设计方法和规范体系。

关键词：桥梁工程 Fr.加固混凝土结构加固方式设计理论综述