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Tolerance and safety evaluation of sodium sulfate:A subchronic study in laying hens

Animal Nutrition 2021年第2期7卷 576-586页

作者： Bing Liu Jiaming Zhu Qin Zhou dongyou yu College of Animal Sciences Zhejiang UniversityHangzhou310058China State Key Laboratory of Food Science and Technology and Synergetic Innovation Center of Food Safety and NutritionJiangnan UniversityWuxi214122China

Sodium sulfate(Na2SO4)is a readily available chlorine-free source of sodium,which could be used to reduce sodium chloride to maintain the ratio between chlorine and sodium in poultry *** supplementation with excessive levels of Na2SO4 might be detrimental to bird's health and per-formance.A subchronic study was carried out to investigate the potential adverse effects of an accidental oversupply of Na2SO4 in the diets of laying *** hundred and fifty 21-week-old Hy-Line White layers were randomly assigned to 5 treatments with 6 *** birds were fed diets supplemented with 0(control),0.3%,0.6%,1.5%,and 3.0%Na2SO4 for 8 *** perfor-mance,egg quality parameters,clinical blood parameters,histopathology,intestinal barrier func-tions,and intestinal microflora composition were *** clinical signs of intoxication or mortality were observed during the experimental *** results of this study showed that the optimal levels of Na2SO4(0.3%to 0.6%)significantly improved the laying rates,average daily egg mass,and eggshell quality of hens compared to the control(P<0.05).However,3.0%Na2SO4 produced negative effects on laying performance,eggshell quality,blood biochemistry,and partic-ularly on liver and kidney pathology,and intestinal morphology and barrier functions compared with the *** minor changes were observed in clinical blood parameters of hens receiving 1.5%Na2SO4,these were not considered to be of toxicological significance due to the absence of any organ pathological changes in *** conclusion,our results indicated that a Na2SO4 concentration of 1.5%was non-deleterious to laying hens after a daily administration for 56 d,namely that dietary supplementation of up to 5 times the maximum recommended dose is safely tolerated by laying hens.

关键词： Sodium sulfate Tolerance Histopathology Intestinal function Laying hen

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Optimal dietary copper requirements and relative bioavailability for weanling pigs fed either copper proteinate or tribasic copper chloride

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Journal of Animal Science and Biotechnology 2020年第4期11卷 1189-1203页

作者： Gang Lin Yang Guo Bing Liu Ruiguo Wang Xiaoou Su dongyou yu Pingli He Institute of Quality Standards and Testing Technology for Agricultural Products Chinese Academy of Agricultural SciencesKey Laboratory of Agrifood Safety and QualityMinistry of Agriculture and Rural AffairsBeijing 10081People’s Republic of China College of Animal Science Zhejiang UniversityKey Laboratory of Animal Nutrition and Feed in East China of Ministry of Agriculture and Rural AffairsHangzhouZhejiang 310058People’s Republic of China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu 214122People’s Republic of China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China.

Background: The objective of this study was to determine the effects of supplementing Cu on growth performance, Cu metabolism and Cu-related enzyme activities of weanling pigs fed diets with two different Cu sources, and to estimate optimal Cu requirements and relative bioavailability from these two sources for ***: Weanling pigs were allocated to 14 treatments arranged factorially, including 6 added Cu levels(5, 10,20, 40, 80, 160 mg/kg), and 2 mineral sources(tribasic Cu chloride, TBCC and copper proteinate, Cu Pro), as well as one negative control(0 mg/kg added Cu level) and one maximum allowed level treatment(200 mg/kg TBCC) for the entire 38-d experiment. Growth performance, mineral status and enzyme activities were measured at the end of this ***: Increasing levels of Cu showed linear and quadratic responses(P 80 mg/kg) and Cu Pro(> 20 mg/kg) significantly decreased(P < 0.05) diarrhea incidence of weanling pigs. There were linear and quadratic increases(P < 0.01) in bile, hepatic,and intestinal Cu concentrations, fecal Cu contents, and plasma enzyme activities(alkaline phosphatase,ceruloplasmin, Cu, Zn-Superoxide dismutase(Cu/Zn SOD), and glutathione peroxidase), whereas plasma malondialdehyde decreased(P < 0.01) linearly and quadratically as dietary Cu level increased. Similarly, pigs fed Cu Pro absorbed and retained more Cu and excreted less Cu than those fed TBCC when supplemented 80 mg/kg and above. Optimal dietary Cu requirements for pigs from 28 to 66 d of age estimated based on fitted broken-line models(P < 0.05) of bile Cu, plasma Cu/Zn SOD and growth performance were 93–140 mg/kg from TBCC, and 63–98 mg/kg from Cu Pro accordingly. According to slope ratios from multiple linear regression, the bioavailability value of Cu Pro relative to TBCC(100%) was 156–263%(P < 0.01).Conclusion: The findings indicated that Cu recommendation from current NRC(5–

关键词： Bioavailability Copper Copper proteinate Excretion Piglet Requirement Tribasic copper chloride

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Probiotic Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 enhance growth performance of broilers by improving the intestinal health

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Animal Nutrition 2021年第3期7卷 829-840页

作者： Baikui Wang Li Gong yuanhao Zhou Li Tang Zihan Zeng Qi Wang Peng Zou dongyou yu Weifen Li Key Laboratory of Molecular Animal Nutrition of the Ministry of Education Key Laboratory of Animal Nutrition and Feed Science(Eastern of China)of the Ministry of AgricultureKey Laboratory of Animal Feed and Nutrition of Zhejiang ProvinceInstitute of Animal Nutrition and Feed SciencesCollege of Animal SciencesZhejiang University310058HangzhouChina Hainan Institute Zhejiang UniversitySanya 572000China

With the ever-growing strict prohibitions on antibiotic growth promoters(AGP)in animal production,in-feed probiotics are becoming attractive alternatives to antibiotics in the poultry *** investigate the effects of Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 on the growth performance and intestinal health of broilers,540 male Cobb 500 broilers of 1 d old were randomly divided into 3 groups with 6 replicates per group and 30 chicks per *** were fed with either a basal diet or basal diets supplemented with 1×108 colony-forming units(CFU)/kg *** 10(BSC10)or *** 16(Lac16)for 42 *** showed that Lac16 treatment improved(P0.05)the growth performance of the starter phase *** increased villus height(P<0.05)at d 14,21 and 42 and villus height to crypt depth ratio(P<0.05)at d 14 and 21 were observed in the ileum of the 2 probiotic ***,transmission electron microscopy results showed that the 2 probiotics enhanced the intestinal epithelial *** probiotic treatments up-regulated(P<0.05)the mRNA expression of fatty acid binding protein 1(FABP1)and sodium-dependent glucose transporters-1(SGLT-1)in the ileal mucosa of broilers at d *** addition,BSC10 and Lac16 treatments significantly(P<0.05)increased the relative abundance of short-chain fatty acids-producing bacteria,such as Butyricicoccus pullicaecorum,Faecalibacterium prausnitzii,Lachnospira and Cop-rococcu,and significantly(P<0.05)decreased the relative abundance of enteric pathogens(Escherichia coli,Bacteroides fragilis and Shigella sonnei).Furthermore,the 2 probiotic treatments also increased the positive connection among the intestinal microbes and the carbohydrate metabolism-related pathways of the intes-tinal bacteria(P<0.05),with decreasing(P<0.05)nucleotides biosynthesis-related pathways of the intesti

关键词： Paenibacillus polymyxa Lactobacillus plantarum Growth performance Intestinal health Broiler

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Dietary supplementation of Macleaya cordata extract and Bacillus in combination improve laying performance by regulating reproductive hormones,intestinal microbiota and barrier function of laying hens

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Journal of Animal Science and Biotechnology 2023年第2期14卷 690-710页

作者： Fei Wang Peng Zou Shujie Xu Qi Wang yuanhao Zhou Xiang Li Li Tang Baikui Wang Qian Jin dongyou yu Weifen Li Key Laboratory of Animal Molecular Nutrition of Education of Ministry National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and ControlKey Laboratory of Animal Feed and Nutrition of Zhejiang ProvinceInstitute of Animal Nutrition and Feed SciencesCollege of Animal SciencesZhejiang UniversityHangzhou 310058China Hainan Institute Zhejiang UniversityYazhou Bay Sci-Tech CityYongyou Industry ParkSanya 572000China

Background:This study aimed to investigate whether the combination of Macleaya cordata extract(MCE)and Bacil-lus could improve the laying performance and health of laying hens ***:A total of 36029-week-old Jingbai laying hens were randomly divided into 4 treatments:control group(basal diet),MCE group(basal diet+MCE),Probiotics Bacillus Compound(PBC)group(basal diet+compound Bacil-lus),MCE+PBC group(basal diet+MCE+compound Bacillus).The feeding experiment lasted for 42 ***:The results showed that the laying rate and the average daily egg mass in the MCE+PBC group were significantly higher than those in the control group(P<0.05)and better than the MCE and PBC ***-tion of MCE and Bacillus significantly increased the content of follicle-stimulating hormone(FSH)in the serum and up-regulated the expression of related hormone receptor gene(estrogen receptor-β,FSHR and luteinizing hormone/choriogonadotropin receptor)in the ovary of laying hens(P<0.05).In the MCE+PBC group,the mRNA expressions of zonula occluden-1,Occludin and mucin-2 in jejunum was increased and the intestinal epithelial barrier detected by transmission electron microscopy was enhanced compared with the control group(P<0.05).In addition,compared with the control group,combination of MCE and Bacillus significantly increased the total antioxidant capacity and catalase activity(P<0.05),and down-regulated the mRNA expressions of inflammation-related genes(interleukin-1βand tumor necrosis factor-α)as well as apoptosis-related genes(Caspase 3,Caspase 8 and P53)(P<0.05).The concen-tration of acetic acid and butyric acid in the cecum content of laying hens in the MCE+PBC group was significantly increased compared with the control group(P<0.05).Conclusions:Collectively,dietary supplementation of 600μg/kg MCE and 5×108 CFU/kg compound Bacillus can improve laying performance by improving microbiota to enhance antioxidant capacity and intestinal barrier,regulate reproductive hormones and the

关键词： Bacillus Intestinal microbiota Laying hens Laying performance Macleaya cordata extract Reproductive hormones

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呈支气管肺炎表现的非典型哮喘分析

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医药论坛杂志 2003年第6期24卷 16-16,18页

作者：于东游河南省滑县人民医院滑县456400

目的探索呈支气管肺炎表现的非典型哮喘的诊治方法。方法对38例呈支气管肺炎表现的非典型哮喘患儿进行临床分析。全部病例均给以静脉点滴氢化考的松4～8mg/(kg.d)连续3～7d;同时口服酮替芬、博利康尼。7d后肺部仍有罗音者改用强的松1m... 详细信息

目的探索呈支气管肺炎表现的非典型哮喘的诊治方法。方法对38例呈支气管肺炎表现的非典型哮喘患儿进行临床分析。全部病例均给以静脉点滴氢化考的松4～8mg/(kg.d)连续3～7d;同时口服酮替芬、博利康尼。7d后肺部仍有罗音者改用强的松1mg/(kg.d)口服,并同时加服654-2,0.5～1mg/(kg.d)。全部病人出院后均采用普米克气雾剂200ug～400ug/d雾化吸入,持续3个月。结果本组患儿有哮喘家族史8例占21％;特应性体质19例,占50％;婴幼儿期患毛细支气管炎9例,占24％。34例应用糖皮质激素7d后症状基本控制,占90％,其余4例加服654-2后症状消失。结论儿童期支气管肺炎经应用抗生素无效者应考虑哮喘,治疗应首选糖皮质激素。

关键词：支气管肺炎非典型哮喘分析糖皮质激素

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大麦型饲粮中添加非淀粉多糖酶对生长猪促生长效应的研究

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中国饲料 2001年第10期 19-20页

作者：王宗沛余东游浙江省宁海县畜牧中心浙江大学饲料所

大麦在谷物饲料中的地位仅次于玉米，其蛋白质、氨基酸、矿物质和维生素含量均超过玉米。尽管大麦的营养价值较高，但由于其含有抗营养因子-非淀粉多糖(NSP)而使畜禽的消化率较低。大麦中的NSP主要为β-葡聚糖、木聚糖和纤维素。目前国... 详细信息

大麦在谷物饲料中的地位仅次于玉米，其蛋白质、氨基酸、矿物质和维生素含量均超过玉米。尽管大麦的营养价值较高，但由于其含有抗营养因子-非淀粉多糖(NSP)而使畜禽的消化率较低。大麦中的NSP主要为β-葡聚糖、木聚糖和纤维素。目前国外已开发出了针对大麦饲粮的酶制剂，这类酶主要是由β-葡聚糖酶、木聚糖酶和纤维素酶组成的NSP酶。东欧等国家通过使用NSP酶制剂已使大麦、小麦等能量饲料在饲料中的用量达70 %。在我国这方面的研究较少，本试验探讨了大麦型饲粮中添加NSP酶对仔猪、生长猪生产性能的影响，旨在为养猪生产中广泛应用添加酶制剂的大麦型日粮提供科学依据。1 材料与方法1.1 试验动物选用浙江省宁海县振兴禽业有限公司的“杜×长×大”三元杂交猪180头，按体重相近、遗传基础相似、性别各半的原则，随机分成对照和试验两组，每组设6个重复，每个重复15头，分别饲养在同一猪舍的12个栏内。

关键词：生长猪大麦型饲粮饲料添加剂非淀粉多糖酶促生长效应生产性能

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生物活性肽对肠道健康的调控机制及其在畜禽生产中的应用

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动物营养学报 2024年第4期36卷 2128-2142页

作者：胡兆莹刘兵曹久爱余东游浙江大学动物科学学院杭州310058 浙江大学海南研究院三亚572025

生物活性肽是一类具有特殊生物学功能的氨基酸序列多肽片段,主要通过发挥抗菌、抗炎、免疫和抗氧化等功能改善机体健康。肠道是动物消化吸收营养物质的主要器官,良好的肠道环境对维持机体健康至关重要。生物活性肽可通过改善肠道形态和... 详细信息

生物活性肽是一类具有特殊生物学功能的氨基酸序列多肽片段,主要通过发挥抗菌、抗炎、免疫和抗氧化等功能改善机体健康。肠道是动物消化吸收营养物质的主要器官,良好的肠道环境对维持机体健康至关重要。生物活性肽可通过改善肠道形态和黏膜功能、增强肠道免疫和抗氧化功能、调节肠道菌群及其代谢产物等途径维持肠道稳态、改善肠道健康。本文综述了常见生物活性肽的功能特性及其对畜禽肠道健康的调控机制,以期为在畜禽生产中的应用和新型生物活性肽的开发提供参考。

关键词：生物活性肽肠道健康调控机制畜禽

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Effect of dietary supplementation of probiotic on performance and intestinal microflora of Chinese soft-shelled turtle(Trionyx Sinensis)

Effect of dietary supplementation of probiotic on performanc...

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中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会暨第五届会员代表大会

作者： Zhang Xiaoping Peng Lisha Wang Yibing Liang Quan Deng Bin Li Weifen Fu Luoqin yu dongyou Shen Wenying Wang Zhiping Key Laboratory of Molecular Animal Nutrition Ministry of EducationCollege of Animal ScienceZhejiang University Department of Biology Life Science College of Shaoxing University College of Agriculture and Biotechnology Zhejiang University

This experiment was conducted to study the effect of dietary supplementation of probiotic on performance,and intestinal microflora of Chinese soft-shelled *** Chinese soft-shelled turtle(6000 pieces with average weight of 5g) were selected and randomly divided into two groups:control and treatment with three replicates *** control was fed on normal basal diet whereas treatment was fed on basal diet supplemented with 1×10 cfu·g Bacillus subtilis *** results showed that the final mean weight of treatment(265.84±3.48 g) was significantly higher(P<0.05) than that of control(240.45±4.32 g).There was significant difference(P<0.05) in FCR and DG were obtained in probiotics fed group as compared to *** level of sucrase,maltase,amylase,lipase and ATPase of treatment were significantly higher(P<0.05) than that of *** showed that the dietary supplementation of probiotic Bacillus strain decreased microbial *** Firmicutes was dominant in both *** with the control,Firmicutes was increased,while others were decreased in treatment *** results indicated that dietary supplementation of probiotic can improve growth performance,digestibility and intestinal microflora in Chinese soft-shelled turtle.

关键词： Probiotic Chinese soft-shelled turtle growth performance digestive enzyme activity intestinal microflora 454-pyrosequencing

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硫酸钠在蛋鸡饲粮中的安全性评价

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动物营养学报 2015年第9期27卷 2856-2862页

作者：熊平文刘兵陈娜娜余东游浙江大学动物科学学院农业部华东动物营养与饲料重点实验室杭州310058

本试验旨在研究饲粮中添加高剂量硫酸钠对蛋鸡生产性能、蛋品质、血液学指标和组织病理学的影响,以确定硫酸钠对蛋鸡饲用的安全性。选取健康且体重和产蛋率相近的20周龄海兰白蛋鸡450只,随机分为5个组,每组6个重复,每个重复15只鸡。各... 详细信息

本试验旨在研究饲粮中添加高剂量硫酸钠对蛋鸡生产性能、蛋品质、血液学指标和组织病理学的影响,以确定硫酸钠对蛋鸡饲用的安全性。选取健康且体重和产蛋率相近的20周龄海兰白蛋鸡450只,随机分为5个组,每组6个重复,每个重复15只鸡。各组分别饲喂硫酸钠添加水平为0（对照组）、0.3%（推荐有效剂量组）、0.6%（2倍推荐有效剂量组）、1.5%（5倍推荐有效剂量组）和3.0%（10倍推荐有效剂量组）的试验饲粮。试验持续10周。结果显示：1）饲粮中添加硫酸钠对日产蛋重、平均蛋重、平均日采食量、产蛋率和料蛋比均无显著影响（P〉0.05）。2）与对照组相比,0.3%和0.6%组各项蛋品质指标有改善趋势（P〉0.05）;与0.6%组相比,高剂量（1.5%和3.0%）组的蛋品质各项指标均显著降低（P〈0.05）。3）各组之间血液常规指标均无显著影响（P〉0.05）。4）与对照组相比,0.3%和0.6%组血浆葡萄糖、尿酸、肌酐、总蛋白、白蛋白、尿素氮和总胆红素含量及谷丙转氨酶、谷草转氨酶和碱性磷酸酶活性均无显著差异（P〉0.05）;与0.6%组相比,高剂量（1.5%和3.0%）组极显著提高了血浆谷丙转氨酶活性（P〈0.01）。5）添加推荐剂量的5倍（1.5%）以上的硫酸钠会引起主要器官组织的损伤和病变。结果提示,饲粮中添加0～0.6%的硫酸钠对蛋鸡饲用是安全的。

关键词：硫酸钠安全性评价蛋鸡饲粮

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利用芽孢杆菌去除水体中亚硝酸氮的研究

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饲料工业 2019年第24期40卷 52-56页

作者：刘韶娜余东游赵智勇云南省畜牧兽医科学院云南昆明650224 浙江大学动物科学学院浙江杭州310058

从甲鱼池底淤泥中筛选的细菌,对其脱氮特性进行研究。实验以芽孢杆菌JY14为研究对象,通过单因子实验测定不同因素对其亚硝酸氮去除的影响。实验表明:菌株JY14在亚硝酸氮浓度为3 mg/l时,1%(v/v)左右的接种量,经30℃、16 h,150 r/min震荡... 详细信息

从甲鱼池底淤泥中筛选的细菌,对其脱氮特性进行研究。实验以芽孢杆菌JY14为研究对象,通过单因子实验测定不同因素对其亚硝酸氮去除的影响。实验表明:菌株JY14在亚硝酸氮浓度为3 mg/l时,1%(v/v)左右的接种量,经30℃、16 h,150 r/min震荡培养,亚硝酸氮去除率可达99.50%。此菌株具有较好的实验室降低亚硝酸氮效果,在去除亚硝酸氮方面有良好的市场前景。

关键词：亚硝酸氮特征去除芽孢杆菌脱氮

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