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Protein identification and mRNA analysis of phyto-chrome-regulated genes in Arabidopsis under red light

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Science China(Life Sciences) 2009年第4期52卷 371-380页

作者： LI Xu YANG YueJun LI Yan WANG Jie XIAO XiaoJuan GUO XinHong tang dongying LIU XuanMing Bioenergy and Biomaterial Research Center College of Life Science and BiotechnologyHunan UniversityChangsha 410082China

Phytochromes are a family of plant photoreceptors that mediate physiological and developmental re- sponses to red and far-red light. According to the affymetrix ATH1 microarray, phytochrome A (phyA) and phytochrome B (phyB) together play a key role in transducing the Rc signals to light-responsive genes. In order to select those red light-responsive genes associated with phyA or phyB, a proteomic approach based on two-dimensional gel electrophoresis (2-DE) was used to compare the protein ex- pression patterns of the phyAphyB double mutant and the wild type of Arabidopsis thaliana (col-4) which grew under constant red light conditions for 7 d. Thirty-two protein spots which exhibited dif- ferences in protein abundance were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of ten genes corresponding to ten protein spots was analyzed by a semiquantitative reverse transcription-polymerase chain reaction. Two of the ten genes were confirmed by quantitative PCR (Q-PCR). The results showed that phytochromes may exert their function by regulating mRNA or protein expressions. Proteomic analysis may provide a novel pathway for identifying phytochrome-dependent genes.

关键词： Arabidopsis thaliana phyAphyB Proteomic RT-PCR Q-PCR

The blue light receptor CRY1 interacts with GID1 and DELLA proteins to repress GA signaling during photomorphogenesis in Arabidopsis

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Molecular Plant 2021年第8期14卷 1328-1342页

作者： Ming Zhong Bingjie Zeng dongying tang Jiaxin Yang Lina Qu Jindong Yan Xiaochuan Wang Xin Li Xuanming Liu Xiaoying Zhao College of Biology Hunan Province Key Laboratory of Plant Functional Genomics and Developmental RegulationHunan Hybrid Rape Engineering and Technology Research CenterHunan UniversityChangsha 410082China Shenzhen Institute Hunan UniversityShenzhen 518057China

Light is a critical environmental cue that regulates a variety of diverse plant developmental *** 1(CRY1)is the major photoreceptor that mediates blue light-dependent photomorphogenic responses such as the inhibition of hypocotyl ***(GA)participates in the repression of photomorphogenesis and promotes hypocotyl ***,the antagonistic interaction between blue light and GA is not well ***,we report that blue light represses GA-induced degradation of the DELLA proteins(DELLAs),which are key negative regulators in the GA signaling pathway,via CRY1,thereby inhibiting the GA response during hypocotyl *** in vitro and in vivo biochemical analyses demonstrated that CRY1 physically interacts with GA receptors-GA-INSENSITIVE DWARF 1 proteins(GID1s)-and DELLAs in a blue light-dependent ***,we showed that CRY1 inhibits the association between GID1s and ***,CRY1 antagonizes the function of GID1s to repress the expression of cell elongation-related genes and thus hypocotyl *** together,our findings demonstrate that CRY1 coordinates blue light and GA signali ng for plant photomorphogenesis by stabilizing DELLAs through the binding and in activation of GID1s,providing new in sights into the mechanism by which blue light antagonizes the function of GA in photomorphogenesis.

关键词： cryptochrome 1 blue light gibberellic acid GID1 DELLA proteins

F-box gene FOA2 regulates GA-and ABA-mediated seed germination in Arabidopsis

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Science China(Life Sciences) 2016年第11期59卷 1192-1194页

作者： Reqing Hei Dashi Yu Xinmei Li Guifang Duan Yunxuan Zhang dongying tang Xiaoying Zhao Xuanming Liu Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan UniversityChangsha 410082China State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan UniversityChangsha 410082China

Dear Editor, GA and ABA antagonize each other in controlling seed germination, but the molecular mechanism is not fully understood. The F-box proteins act as the most important SCF （SKP1, cullin/CDC53, F-box protein） complex subunit of the ubiquitin （Ub）- 26S proteasome system, mediate diverse physiological processes ranging from hormonal signaling cascades to environmental stress responses （Santner and Estelle, 2010）. We previously demonstrated that FOAl （F-box overexpressed/oppressed ABA signaling） is an ABA signaling-related gene,

关键词： and ABA mediated seed germination in Arabidopsis FOA F-box gene FOA2 regulates GA Figure gene

The PHYB–FOF2–VOZ2 module functions to finetune flowering in response to changes in light quality by modulating FLC expression in Arabidopsis

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Plant Communications 2024年第7期5卷 231-250页

作者： Lina Qu Ming Zhong Feifei Duan Xinmei Li Jiaxin Yang Quanyu Zhou dongying tang Reqing He Xuanming Liu Xiaoying Zhao College of Biology Hunan Province Key Laboratory of Plant Functional Genomics and Developmental RegulationHunan Engineering and Technology Research Center of Hybrid RapeseedHunan UniversityChangsha 410082China Shenzhen Institute Hunan UniversityShenzhen 518057China These authors contributed equally to this article

Proper timing of flowering under different environmental conditions is critical for plant *** quality is a pivotal environmental cue that plays a critical role in flowering *** tend to flower late under light with a high red(R)/far-red(FR)light ratio but early under light with a low R/FR light ***,how plants fine-tune flowering in response to changes in light quality is not well ***,we demonstrate that F-box of Flowering 2(FOF2),an autonomous pathway–related regulator,physically interacts with VASCULAR PLANT ONE-ZINC FINGER 1 and 2(VOZ1 and VOZ2),which are direct downstream factors of the R/FR light receptor phytochrome B(PHYB).We show that PHYB physically interacts with FOF2,mediates stabilization of the FOF2 protein under FR light and end-of-day FR light,and enhances FOF2 binding to VOZ2,which leads to degradation of VOZ2 by SCF^(FOF2) E3 *** contrast,PHYB mediates degradation of FOF2 protein under R light and end-of-day R *** interaction studies demonstrated that FOF2 functions downstream of PHYB to promote FLC expression and inhibit flowering under both high R/FR light and simulated shade conditions,processes that are partially dependent on VOZ *** together,our findings suggest a novel mechanism whereby plants fine-tune flowering time through a PHYB–FOF2–VOZ2 module that modulates FLC expression in response to changes in light quality.

关键词： PHYB FOF2 VOZ proteins FLC light quality flowering time

Arabidopsis F-box gene FOA1 involved in ABA signaling

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Science China(Life Sciences) 2012年第6期55卷 497-506页

作者： PENG Juan YU DaShi WANG LiQun XIE MinMin YUAN CongYing WANG Yu tang dongying ZHAO XiaoYing LIU XuanMing College of Life Sciences State Key Laboratory of Chemo/Biosensing and ChemometricsHunan UniversityChangsha 410082China

The expression of FOAl （F-box overexpressed/oppressed ABA signaling） in different organs of Arabidopsis, and in response to ABA and NaCI, was analyzed. The expression level of FOAl is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaC1. The phenotypes of T-DNA insertion mutant foal and FOAl overexpression lines FOAloxl and FOAlox2 were analyzed. The foal mutant exhibited a lower germination rate, shorter root length, more stomatal opening, in- creased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foal mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foal mutant. This study demonstrates that FOAl is an ABA signaling-related gene, and may play a negative role in ABA signaling.

关键词： F-box gene FOAl ABA signaling Arabidopsis

Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

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Journal of Integrative Plant Biology 2018年第2期60卷 85-88页

作者： dongying tang Yuchong Peng Jianzhong Lin Changqing Du Yuanzhu Yang Dan Wang Cong Liu Lu Yan Xiaoying Zhao Xia Li Liangbi Chen Xuanming Liu College of Life Sciences Hunan Normal University Changsha 410081 China Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Biology Hunan University Changsha 410082 China Academy of Seed Industry of Hunan Yahua Changsha 410001 China

NADP（H）-dependent glutamate dehydrogenases（GDH） in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen *** results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.

关键词： GDH Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

Efficient Transformation of indica Rice Mediated by Agrobacterium and Generation of NcGDH Transgenic Genic Male-Sterile Rice with High Nitrogen Use Efficiency

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Rice science 2021年第6期28卷 529-531,I0030-I0036页

作者： LIU Cong tangg dongying ZHOU Zhengkun ZENG Hui HU Xiaochun TAN Vanning QIN Peng DENG Yong WU Jicai WANG Yan YANG Yuanzhu YUAN Dingyang LIU Xuanming LIN Jianzhong Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan UniversityChangsha 410082China Academy of Seed Industry of Hunan Yahua Changsha 410001China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterHunan Academy of Agricultural SciencesChangsha 410125China

Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica ***,a fast and efficient method of Agrobacterium-mediated transformation for indica rice was developed,and an NcGDH transgenic genic male-sterile(GMS)indica rice 628S was generated using this *** regenerated transgenic plantlets can be obtained within three months,and the transformation efficiency achieved about 17%.NcGDH transgenic 628S exhibited an enhanced capacity of ammonium assimilation and more effective panicles under low nitrogen *** the hybrid rice lines generated by NcGDH transgenic 628S showed 8%-27%yield advantage than those by the non-transgenic *** together,these results provide an efficient transfbnnation method for indica rice and an opportunity to improve nitrogen use efficiency(NUE)and the heterosis of hybrid rice.

关键词： sterile Transformation transformation

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FR-4覆铜板专用溴化环氧树脂合成新工艺

化工进展 2005年第4期24卷 441-444页

作者：汤冬英邓海波巴陵石油化工有限责任公司岳阳414014

研究了原料配比、反应温度、反应时间、催化剂等因素对溴化环氧树脂合成新工艺主反应的影响。结果表明,合成新工艺的主反应温度比传统工艺高10～30℃,催化剂用量为四溴双酚A加入量的0.1%,反应时间为120～180min,合成的中间体溴化环氧树... 详细信息

研究了原料配比、反应温度、反应时间、催化剂等因素对溴化环氧树脂合成新工艺主反应的影响。结果表明,合成新工艺的主反应温度比传统工艺高10～30℃,催化剂用量为四溴双酚A加入量的0.1%,反应时间为120～180min,合成的中间体溴化环氧树脂的环氧当量比成品的要高300～500g/mol。同时,对产品质量指标进行了综合分析,并对其相对分子质量分布进行了剖析。其结果表明,产品的环氧当量、固体含量、溶液黏度等指标均达到最佳范围,分子组成与进口产品相当。

关键词：覆铜板溴化环氧树脂工艺技术改进

Arabidopsis F-box gene At3g16740 involving in light-,GA-and ABA-mediated seed germination

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Arabidopsis F-box gene At3g16740 involving in light-,GA-and ...