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A Pathogenic Fungus Phoma *** Identified from Tea Leaf Spot Disease in Shiqian County,Guizhou Province,China

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A Pathogenic Fungus Phoma *** Identified from Tea Leaf Spot ...

中国植物病理学会2017年学术年会

作者： Zhao Xiaozhen li dongxue Bao Xingtao Duan Changliu Ren Yafeng li Xiangyang Yu Haiyou Yang Song Chen Zhuo Song Baoan Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University

The tea leaf spot disease is always taken place in the region with the characteristics of the low-temperature and high-humidity containing with Shiqian County,Guizhou *** disease always infects the tender leaves and shoots,which lead to the lowest tea quality and the significant production *** identify the pathogen of the tea spot disease,this work isolated and identified the pathogen of this disease from the tea leaves,and determined the pathogenicity of the pathogen according to Koch’s *** study indicated that the isolated strain of GZSQ-4 represent the features as following;on PDA medium,margin regular of the colonies,aerial mycelium closely growing,white mycelium in the early stage;then gradually change to grey brown,black brown in the center,with white margin in the latter stage;reverse brown to black brown near the center,with the white *** OA medium,margin regular of the colonies,aerial mycelium woolly,pale brown to brown near the center,and produce a lot of black *** the optical microscope and SEM indicated that the septate mycelium contains the inclusion granule;Pycnidia represent subglobose,glabrous,thin-walled,solitary or confluent,50 190μm,1 or 2 ostioes with a neck;Conidia represent ellipsoidal or ovoid,thin-walled,smooth,hyaline,aseptate,(2) μm×(1 .5) μm,with 0 guttules in two polar *** genes of ITS,β-tubulin and LSU were further amplified,purified and sequenced,then the phylogenetic trees were constructed using MEGA6.0 software and Neighbor-joining *** results indicated that the strainof GZSQ-4 was closely related to the strains of Phoma ***-2014 b(LC 1633) and Didymel-1 a bellidis(CBS 714.85).Integrated with the above results,the pathogen isolated by our research group are preliminarily identified to Phoma sp..

关键词： Phoma sp Tea leaf spot disease Pathogenic fungus

The Identification of Tea Gray Blight Disease in Huishui County,Guizhou Province,China

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The Identification of Tea Gray Blight Disease in Huishui Cou...

中国植物病理学会2017年学术年会

作者： li dongxue Zhao Xiaozhen Bao Xingtao Ren Yafeng Wang Yong li Xiangyang Yu Haiyou Tan Xiaofeng Chen Zhuo Song Baoan Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University Agricultural College Guizhou University

Tea gray blight disease is an important disease of the tea tree,which can harm tea seedlings,as well as the shoots,fresh leaves and mature leaves of trees for various growth *** disease can cause the tea plants weakened and severethe production loss of tea *** gray blight disease is one of the most important diseases in Guizhou tea *** this work,the pathogenic fungus was isolated,purified from the diseased tea leaves,and confirmed the pathogenicity of the pathogen according to Koch’s *** isolated strain of DX-1 was further identified by the morphology and molecular *** study indicated that DX-1 represents the features as following;on PDA medium,the colonyis obviously wheel round like,margin regular of the colonies;Reverse represents light yellow colony with white or colorless *** observation using the optical microscope and SEM indicated that theconidia represent fusi form with 4 diaphragms and 2 thin ends,and 2-3 appendages and1 appendage exist onthe top and the tail end of the cell,*** with（16.07±1.24） μm×（4.23±0.20） μm is colorless in the early stage,and the 3 septum near the center change brown in the late *** genes of EF-1α,β-tubulin and LSU were further amplified,purified and sequenced,and then the phylogenetic trees were constructed using MEGA6.0 software according to Neighbor-joining *** results indicated that DX-1 strain was closely related to Pestalotiopsis ***1（KJ623232.1） and YN1 B4（KU252565.1） against two phylogenetic treesof EF-1α and β-tubulin;and then clustered together with *** strain 3.919 2（JN940840.1） in a branch,and closely related to *** stain 3.909 8（JN940825.1） for the phylogenetic tree of LSU.

关键词： Tea gray blight disease Pathogenicity Pestalotiopsis theae

Nemania bipapillata was firstly identified from tea leaf spot disease in Yuqing,Guizhou Province,China

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Nemania bipapillata was firstly identified from tea leaf spo...

中国植物病理学会2019年学术年会

作者： Wang Xue Yin Qiaoxiu li dongxue Ren Yafeng Wu Xian Jiang Shilong Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Tea plant(Camellia sinensis) is a major crop in Guizhou province,*** the end of 2017,the cultivated area of tea plant covered 350 000 *** November 2018,tea leaf spot was caused a huge loss of the production of tea in Yuqing county in Guizhou *** symptoms of the infected tea leaves were brown,irregular and the center of lesion appeared to be *** some tea gardens,disease incidence of leaves was 46% to 52%,and decreased seriously the production of tea *** this study,some fungi from tea disease samples were identified,such as Phomopsis foeniculi,Diaporthe ganias,et ***,some isolates were obtained to our *** colony of GZYQ-03-02 on PDA initially was white,and then the colony was gradually changed to black after 14 days *** genes of DNA-dependent RNA polymerase II second largest subunit(RPB2),beta-tubulin 2(β-TUB2) and actin(ACT) were amplified and sequenced from the isolates,and then deposited in Genbank(Accession ***852276,MK852273 and MK852274 for the isolate GZYQ-03-02).Maximum parsimony phylogenetic analysis based on concatenated sequences of combined ACT(1-312),β-TUB2(313-506),RPB2(507-847) indicated that the isolate GZYQ-03-02 was identical to reference strains Nemania bipapillata 90080610,and the clade was supported by 99% bootstrap *** fulfill Koch’s postulates,unwounded and wounded tea leaves were inoculated with mycelial plugs on PDA,and non-symptoms were observed after 7 days of *** was known as synonym of Nemania *** caused tea stem wood decay in Sri ***,*** was also known as endophyte,which was isolated from plant hosts of Lycium chinense,Cephalotaxus,Vanilla planifolia,et *** our knowledge,we firstly isolated the fungus from tea *** mechanism of the fungus in the tea leaf spot is worth studying in the future.

关键词： Nemania bipapillata identification pathogenicity test leaf spot pathogenicity mechanism

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黏质路基土永久变形改进计算方法

同济大学学报（自然科学版） 2013年第3期41卷 386-389,457页

作者：李冬雪凌建明钱劲松王海林同济大学道路与交通工程教育部重点实验室上海201804

通过室内重复荷载三轴试验,测试了36种工况下黏质路基土永久应变与试验加载次数之间的关系;采用统计回归的方法对黏质路基土永久变形预估模型进行了标定与修正.以分层应变总和法为基础,结合道路渠化交通的考虑,提出了分条分层总和法作... 详细信息

通过室内重复荷载三轴试验,测试了36种工况下黏质路基土永久应变与试验加载次数之间的关系;采用统计回归的方法对黏质路基土永久变形预估模型进行了标定与修正.以分层应变总和法为基础,结合道路渠化交通的考虑,提出了分条分层总和法作为柔性路面路基土在车辆重复荷载作用下永久变形的改进计算方法,并详细说明该方法的计算原理及步骤.结合典型柔性路面结构实例,计算了黏质路基土的永久变形量,并通过对比分析,验证了该方法的合理性.

关键词：柔性路面黏质路基土永久变形分条分层总和法

Pithomyces chartarum was firstly identified from tea leaf spot in Yuqing,Guizhou Province

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Pithomyces chartarum was firstly identified from tea leaf sp...

中国植物病理学会2019年学术年会

作者： Yin Qiaoxiu Wang Xue Ren Yafeng li dongxue Jiang Shilong Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Leaf spot disease is an important disease of tea(Camellia sinensis) in Yuqing county,Guizhou *** symptoms were small,round or irregular *** lesion gradually enlarged to display the darker lesion margin with a bright yellowish-black *** center of lesion appeared to be *** adjacent lesions have been merged to the larger lesion and covered the margin of the *** disease takes place in tea garden during autumn on altitude from one thousand to one thousand three hundred *** incidence of leaves was estimated at 74% to 82%,depending on the *** severity on a plant basis was estimated to be 68% to 76%.In order to identify the pathogens of this disease,we isolated and identified some fungi from tea samples with the symptoms of leaf spot,and conducted the pathogenicity *** found Phoma segeticola,Didymella bellidis and Pestalotiopsis trachicarpicola cause the lesions of tea leaves by Koch’s *** addition,we also isolated and identifed the isolate of GZYQ4-1-d from the samples of tea *** colony of GZYQ4-1-d on PDA was regular and round,flat growing,sometimes floccose,*** time,the colony became grey to dark grey because the pigment of mycelium *** are typically pigmented,muriform,sometimes echinulate,usually with 3 transverse septa and with 0-2 longitudinal septa,measured to(17.16-28.38) μm×(12.57-18.04) μ*** internal transcribed spacer(ITS) regions 1 and 2 was used to amplify and sequenced,and the sequence revealed 100% identity with *** by Blastn *** pathogenicity test was conducted result indicated that the strains can induce leaf spot on leaves of *** leaves of the tea plant varieties of Moss tea and Fuding Dabaicha were inoculated with mycelial plugs on PDA or the spore suspension(1×10 spores per mL) using the methods of unwound,puncture or *** symptom has not been observed on inoculated leaves for three ***,some literatures reported t

关键词： Pithomyces chartarum identification pathogenicity test leaf spot pathogenicity mechanism

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湿度循环下黏质路基土回弹模量演化规律

同济大学学报（自然科学版） 2013年第7期41卷 1051-1055页

作者：李冬雪凌建明钱劲松王海林同济大学道路与交通工程教育部重点实验室上海201804

历经多次干湿循环的路基土,其力学性能劣化现象非常突出,路基的路用性能也随之衰减.基于室内试验研究含水率周期性变化对路基回弹模量的影响规律.遵循现场测试含水率变化态势,利用"透水石渗水—静置—烘箱脱水—透水石渗水—静置—烘箱... 详细信息

历经多次干湿循环的路基土,其力学性能劣化现象非常突出,路基的路用性能也随之衰减.基于室内试验研究含水率周期性变化对路基回弹模量的影响规律.遵循现场测试含水率变化态势,利用"透水石渗水—静置—烘箱脱水—透水石渗水—静置—烘箱脱水……"一系列湿度循环来模拟现场路基土的含水率波动过程,同时测试其回弹模量.结果表明:黏质路基土回弹模量受含水率变化的影响非常明显,回弹模量随湿度循环次数的增大而减小;在相同的应力状态下,黏质路基土的平衡含水率越高,其回弹模量值对含水率的周期性变化越敏感,且回弹模量值趋于不稳定状态.

关键词：黏质路基土回弹模量含水率湿度循环

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陕西省城市地下空间开发利用规划研究

西北大学学报（自然科学版） 2017年第2期47卷 277-282页

作者：李冬雪刘科伟西北大学城市与环境学院陕西西安710127

城市地下空间开发利用规划是促进地下空间合理有序开发的重要保证。文章通过对陕西省城市地下空间开发条件进行评价,认为全省三大地理区地下空间开发条件呈现明显的地域性特征,并在建立数学模型预测城市地下空间需求量的基础上,明确各... 详细信息

城市地下空间开发利用规划是促进地下空间合理有序开发的重要保证。文章通过对陕西省城市地下空间开发条件进行评价,认为全省三大地理区地下空间开发条件呈现明显的地域性特征,并在建立数学模型预测城市地下空间需求量的基础上,明确各城市地下空间分层分区开发要求和不同功能地下设施的开发建设重点,以实现城市地下空间科学有序地开发利用。

关键词：陕西省地下空间开发利用

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氟喹诺酮类抗菌药物发生不良反应的影响因素

中国民康医学 2022年第21期34卷 1-4页

作者：李东学辽宁省健康产业集团沈煤总医院药械科辽宁沈阳110122

目的:探讨氟喹诺酮类抗菌药物发生不良反应的影响因素。方法:回顾性分析2020年1—12月该院收治的156例应用氟喹诺酮类抗菌药物治疗患者的临床资料。依据不良反应发生情况,将发生不良反应患者纳入发生组,将未发生不良反应患者纳入未发生... 详细信息

目的:探讨氟喹诺酮类抗菌药物发生不良反应的影响因素。方法:回顾性分析2020年1—12月该院收治的156例应用氟喹诺酮类抗菌药物治疗患者的临床资料。依据不良反应发生情况,将发生不良反应患者纳入发生组,将未发生不良反应患者纳入未发生组,收集两组临床资料,分析氟喹诺酮类抗菌药物发生不良反应的影响因素。结果:156例应用氟喹诺酮类抗菌药物治疗患者中,31例发生不良反应,发生率为19.87%;其中以皮肤系统、消化系统不良反应居多,且多为单系统不良反应。两组性别、原发病类型和合并高血压占比比较,差异均无统计学意义(P>0.05);发生组年龄≥60岁、合并糖尿病、应用左氧氟沙星、静脉给药、联合用药、用药间隔1,P<0.05)。结论:年龄≥60岁、合并糖尿病、应用左氧氟沙星、静脉给药、联合用药、用药间隔<12 h、大剂量用药均为氟喹诺酮类抗菌药物发生不良反应的危险因素。

关键词：氟喹诺酮类抗菌药物不良反应静脉给药左氧氟沙星用药间隔大剂量用药

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中俄森林保护若干法律制度的比较研究

世界林业研究 2006年第2期19卷 68-73页

作者：包玉华李冬雪东北林业大学哈尔滨150040

从中俄森林保护法律体系若干法律制度的比较中,总结一些有益于我国森林保护的法律措施,希望对我国森林保护法律制度的建设能有所借鉴。

关键词：中国俄罗斯森林保护法律制度比较研究