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Proliferating cell nuclear antigen of Ulva prolifera is involved in the response to temperature stress

Journal of Oceanology and Limnology 2024年第4期42卷 1227-1241页

作者： Hongyan HE Juanjuan YANG Yuan HE Xiuwen YANG Caiwei FU dongren zhang Jingwei DONG Aurang ZEB Jing QU Songdong SHEN School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Department of Pathology Affiliated Hospital and Medical School of Nantong UniversityNantong 226001China

Ulva prolifera is the most common specie causative to green tide,and its growth is sensitive to temperature ***,the mechanisms of *** response to temperature stress remain *** this study,high temperature(36℃)stimulus promoted the death of unformed cell wall protoplasts and delayed the division of formed cell wall protoplasts,while low-temperature(4℃)stimulus did not,suggesting that the mechanisms of the response of *** to high and low temperature stresses are *** results show that proliferation-related genes were differentially expressed under high and low-temperature stresses,especially the proliferating cell nuclear antigen(PCNA)and cyclins(CYCs).Subsequently,the interaction between PCNA and Cyclin A was confirmed by Co-immunoprecipitation,yeast two-hybrid,and so ***,high-and low temperature stresses induced the expression of PCNA and Cyclin A in varying of degrees,and activated extracellular signal-regulated kinase(ERK)signal *** results suggest,PCNA,Cyclin A,and ERK signal pathway played important roles in the resistance of *** to temperature ***,high-temperature stress induced an increase of miR-2916 in abundance,and exhibiting reverse expression of PCNA;and PCNA was target gene of miR-2916,suggesting that miR-2916 protected *** from high-temperature stress via post-transcriptionally regulation of *** study laid a foundation for understanding the function of PCNA and Cyclin A,moreover,it has a guiding significance to explore the mechanisms of the response to temperature stress from proliferation-related genes regulatory networks in ***.

关键词： UIva prolifera proliferating cell nuclear antigen Cyclin A extracellular signal-regulated kinase microRNA temperature stress

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Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins

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Journal of Oceanology and Limnology 2023年第5期41卷 1887-1899页

作者： Zhiyong LI Yehua WANG Caiwei FU dongren zhang Tuanjie CHE Songdong SHEN School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province Lanzhou 730030China

The main protease(M^(pro))is essential for the replication of SARS-COV-2 and therefore represents a promising anti-viral *** this study,we screened M^(pro)inhibitory peptides from Ulva prolifera protein on in-silico *** analysis using the online toxic prediction tool ToxinPred revealed that all the peptides were *** hexapeptide(SSGFID)exhibited high M^(pro)inhibitory activity in molecular docking and its IC_(50)value was 139.40±0.82μmol/L in vitro according to fluorescence resonance energy transfer assay(FRET).Quantitative real-time(qRT-)PCR results show that SSGFID could stimulate the expression of mitosis-related factors,including nuclear factor-κB,cyclin D1,and cyclin-dependent kinase 4,to promote the proliferation of mice *** study revealed that SSGFID showed resistance against pepsin and trypsin but lost D(Asp)after pretreatment at121℃ for 15 ***,SSGFID was mainly transported through the Caco-2 cell monolayer by the peptide transporter PepT1 and passive-mediated transport during the transport ***,the peptide was also degraded by Caco-2 intracellular enzymes,and the transfer rate of intact peptide was4.2%.Furthermore,Lineweaver–Burk plots demonstrated that SSGFID possessed a mixed inhibitory characteristic with M^(pro).Our study indicated the potential of Ulva prolifera as antiviral and immuneenhancing functional food ingredients and nutraceuticals.

关键词： M^(pro)inhibitory peptide Ulva prolifera protein in-silico proteolysis immunomodulatory peptide gastrointestinal digestion transport study

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Study of screening,transport pathway,and vasodilation mechanisms on angiotensin-Ⅰconverting enzyme inhibitory peptide from Ulva prolifera proteins

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Acta Oceanologica Sinica 2023年第11期42卷 98-106页

作者： Zhiyong Li Yuan He Hongyan He Caiwei Fu Mengru Li Aiming Lu dongren zhang Tuanjie Che Songdong Shen School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province Lanzhou 730030China

In this study,Ulva prolifera protein was used for preparing angiotensin-I converting enzyme(ACE)-inhibitory peptide via virtual gastrointestinal digestion and in silico *** parameters of the obtained peptide,such as inhibition kinetics,docking mechanism,stability,transport pathway,were explored by Lineweaver-Burk plots,molecular docking,in vitro stimulate gastrointestinal(GI)digestion and Caco-2 cells monolayer model,***,a novel anti-ACE peptide LDF(IC_(50),(1.66±0.34)μmol/L)was screened and synthesized by chemical *** was a no-competitive inhibitor and its anti-ACE inhibitory effect mainly attributable to four Conventional Hydrogen Bonds and Zn701 *** could keep activity during simulated GI digestion in vitro and was transported by peptide transporter PepT1 and passive-mediated ***,it could activate Endothelial nitric oxide synthase(eNOS)activity to promote the production of NO and reduce Endothelin-1(ET-1)secretion induced by AngiotensinⅡ(AngⅡ)in Human Umbilical Vein Endothelial Cells(HUVECs).Meanwhile,it could promote mice splenocytes proliferation in a concentration-dependent *** study indicated that this peptide was a potential ingredient functioning on vasodilation and enhancing immunity.

关键词： Ulva prolifera protein ACE-inhibitory peptide in vitro GI digestion vasodilation mechanism immunomodulation

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基于道路交通安全因素的交通事故分析

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城市交通 2006年第4期4卷 76-79页

作者：张东仁温州市交通警察支队温州325000

总结归纳了影响道路交通安全的因素:道路交通设施、季节、气候等。以滨海大道和机场大道为例,依据道路主体工程和交通量,分析了交通事故成因,同时提出了相应的整改措施。科学认识了道路交通事故的诱发因素,指出了驾驶人群体性质的变化... 详细信息

总结归纳了影响道路交通安全的因素:道路交通设施、季节、气候等。以滨海大道和机场大道为例,依据道路主体工程和交通量,分析了交通事故成因,同时提出了相应的整改措施。科学认识了道路交通事故的诱发因素,指出了驾驶人群体性质的变化和驾驶人在驾驶过程中感官的极限性对交通安全的影响,从而提出道路规划设计要“以人为本”,行车安全应以“预防为主”的理念。

关键词：交通安全事故分析道路规划交通设计

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提高变电站瓷瓶绝缘强度新工艺

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电工技术 2007年第2期 12-13页

作者：张栋仁福建省永安煤业有限责任公司供电所福建永安366019

结合我公司2座35kV变电站的实际情况,对35kV变电站户外电气设备绝缘降低的原因进行分析,提出4种处理对策。

关键词：绝缘对策防污增爬裙 RTV涂料

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Flow characteristics simulation of spiral coil reactor used in the thermochemical energy storage system

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Chinese Journal of Chemical Engineering 2022年第2期35卷 364-379页

作者： Xiaoyi Chen Danyang Song Dong zhang Xiaogang Jin Xiang Ling dongren Liu School of Mechanical and Power Engineering Nanjing Tech UniversityNanjing 211816China School of Mechanical Engineering Shanghai Institute of TechnologyShanghai 201418China Mechanical Engineering College Yangzhou UniversityYangzhou 225009China

According to environmental and energy issues,renewable energy has been vigorously *** solar power is widely used in many areas but it is limited by the weather conditions and cannot work *** storage is a considerable solution for this problem and thermochemical energy storage is the most promising way because of its great energy density and ***,this technology is not mature enough to be applied to the *** reactor is an important component in the thermochemical energy storage system where the charging and discharging process *** this paper,a spiral coil is proposed and used as a reactor in the thermochemical energy storage *** advantages of the spiral coil include simple structure,small volume,and so *** investigate the flow characteristics,the simulation was carried out based on energy-minimization multi-scale model(EMMS)and Eulerian two-phase ***_(3) particles were chosen as the *** distribution was shown in the *** gas initial velocity was set to 2 m·s^(-1),3 m·s^(-1),and 4 m·s^(-1).When the particles flowed in the coil,gravity,centrifugal force and drag force influenced their *** the Reynold numbers increasing,centrifugal and drag force got *** phenomenon existed in the coil and results showed with the gas velocity increasing,accumulation moved from the bottom to the outer wall of the ***,the accumulation phenomenon was stabilized whenφ>720°.Also due to the centrifugal force,a secondary flow formed,which means solid particles moved from the inside wall to the outside *** secondary flow could promote turbulence and mixing of particles and *** addition,when the particle volume fraction is reduced from 0.2 to 0.1,the accumulation at the bottom of the coil decreases,and the unevenness of the velocity distribution becomes larger.

关键词： Thermochemical energy storage CaCO3/CaO Reactors Simulation Two-phase flow Energy-minimization multi-scale model(EMMS)

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改善温州城区道路交通拥堵状况的建议

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城市建筑 2014年第4期11卷 228-228页

作者：张东仁瑞安市城乡规划设计研究院温州325200

道路交通拥堵是绝大多数城市的通病,它既延误出行又加剧环境污染,大大降低了居住在当地民众的生活幸福指数。本文主要就改善温州城区道路交通拥堵状况提出了自己的一些建议,可供同行参考。

关键词：改善道路交通拥堵状况建议

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重点产业政策能否有效治理产能过剩?

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产业经济评论 2024年第5期 23-37页

作者：张乐音麦东仁余靖雯北京外国语大学国际商学院北京大学光华管理学院

重点产业政策是推动中国产业转型升级、促进经济高质量发展的重要政策工具,在现实中被广泛使用,但关于其有效性却始终无法达成共识。本文基于产能过剩治理视角,对中国重点产业政策的实际作用进行讨论。本文选取“十一五”至“十四五”... 详细信息

重点产业政策是推动中国产业转型升级、促进经济高质量发展的重要政策工具,在现实中被广泛使用,但关于其有效性却始终无法达成共识。本文基于产能过剩治理视角,对中国重点产业政策的实际作用进行讨论。本文选取“十一五”至“十四五”规划中的重点产业政策作为依据,以2007—2022年制造业上市公司作为样本,探讨了中国重点产业政策对企业产能过剩的影响,为合理运用产业政策提供思路借鉴,也为治理产能过剩提供政策启示。研究发现:(1)重点产业政策能够有效治理产能过剩,创新是重点产业政策化解企业产能过剩的影响渠道。(2)地方政府重点产业政策可以有效缓解产能过剩,但中央政府重点产业政策效果却不明显。(3)异质性分析结果表明,重点产业政策对非国有企业和东部地区企业产能过剩的治理更具效力。

关键词：重点产业政策产能过剩创新

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非胶组分对天然橡胶性能的影响研究

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橡胶工业 2024年第7期71卷 483-493页

作者：郭煜雯赵帅鲍柯旭韩冬仁吴晓辉韩冬礼张振翀张继川北京化工大学材料科学与工程学院先进弹性体材料研究中心北京100029 杭州国仁机电工程有限公司浙江杭州311200 弹性体材料节能和资源化教育部工程研究中心北京100029 北京市先进弹性体工程技术研究中心北京100029 淄博国创中心先进车用材料技术创新中心山东淄博255020 山东轻化致远新材料科技有限公司山东淄博256100

天然橡胶(NR)中非胶组分对其分子结构和性能有很大影响,在NR的分子结构构建过程中有助于NR分子链由线性结构变为网状结构。传统烟胶片和标准胶的制备过程采用酸凝工艺,加之剪切造粒和高温干燥等加工方式,均造成大量非胶组分的流失。本... 详细信息

天然橡胶(NR)中非胶组分对其分子结构和性能有很大影响,在NR的分子结构构建过程中有助于NR分子链由线性结构变为网状结构。传统烟胶片和标准胶的制备过程采用酸凝工艺,加之剪切造粒和高温干燥等加工方式,均造成大量非胶组分的流失。本工作采用薄膜干燥法制备的NR在极大程度上避免了非胶组分的流失,通过此法制备的全组分NR胶料表现出良好的力学性能,与分别去除各非胶组分的NR进行性能对比发现:脱除磷脂导致NR胶料的门尼粘度降低26.92%,硫化诱导期缩短24.91%,耐老化性能有所提高;脱除蛋白质导致NR胶料的门尼粘度降低1.72%,硫化诱导期缩短50%,耐老化性能有一定程度提高。

关键词：天然橡胶非胶组分蛋白质磷脂门尼粘度耐老化性能

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DEGRADATION AND PROCESSING OF PLA AND ITS APPLICATION IN FIXATION OF BONE FRACTURE

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Chinese Journal of Reactive Polymers 1999年第1期8卷 102-109页

作者： HU Yushan BAI dongren zhang Zhengpu WANG Yongmei HE Binglin State Key Laboratory of Functional Polymeric Materials for Adsorption and Separation Institute of Polymer Chemistry Nankai UniversityTianjin 300071 Department of Chemistry Nankai UniversityTianjin 300071

PLA is presently considered as the most attractive compound for temporarytherapeutic application in the biomedical field. In this paper we give an overview of thepresent knowledge on the degradation behavior,processin... 详细信息

关键词： PLA Degradation Processing Bone fracture fixation Review

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