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GoldCLIP： Gel-omitted Ligation-dependent CLIP

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Genomics, Proteomics & Bioinformatics 2018年第2期16卷 136-143页

作者： Jiaqi Gu Ming Wang yang yang Ding Qiu Yiqun Zhang Jinbiao Ma Yu Zhou Gregory J. Hannon yang Yu State Key Laboratory of Genetic Engineering Department of Biochemistry School of Life Sciences Fudan University Shanghai 200438 China CAS Key Laboratory of RNA Biology Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University Wuhan 430072 China Cancer Research UK Li Ka Shing Centre University of Cambridge Cambridge CB2 ORE United Kingdom

Protein-RNA interaction networks are essential to understand gene regulation control. Identifying binding sites of RNA-binding proteins （RBPs） by the UV-crosslinking and immunoprecipitation （CLIP） represents one of the most powerful methods to map protein RNA interactions in vivo. However, the traditional CLIP protocol is technically challenging, which requires radioactive labeling and suffers from material loss during PAGE-membrane transfer procedures. Here we introduce a super-efficient CLIP method （GoldCLIP） that omits all gel purification steps. This nonisotopic method allows us to perform highly reproducible CLIP experiments with polypyrimidine tract-binding protein （PTB）, a classical RBP in human cell lines. In principle, our method guarantees sequencing library constructions, providing the protein of interest can be successfully crosslinked to RNAs in living cells. GoldCLIP is readily applicable to diverse proteins to uncover their endogenous RNA targets.

关键词： RNA binding protein UV crosslinking CLIP HaloTag PTB

EFFECTS OF POLYTROPIC EXPONENT ON DATA PROCESSING METHODS USED IN CONSTANT VOLUME DISCHARGE

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Chinese Journal of Mechanical Engineering 2008年第1期21卷 53-56页

作者： yang Lihong LIU Chengliang ZHA Xuanfang School of Mechanical Engineering University of Shanghai for Science and Technology Shanghai 200093 China School of Mechanical EngTneering Shanghai Jiaotong University Shanghai 200240 China University of Maryland MD 20742 USA

In order to obtain the accurate sonic conductance of solenoid valves, the polytropic exponent is used in the data processing of the discharge method for measuring the flow rate characteristics of pneumatic components. Three data processing principles are first introduced, and then the discharge pressure data obtained from the measured solenoid valve are processed to obtain the sonic conductance with three methods： the partial polytropic exponent method, the complete polytropic exponent method and the adiabatic method. By comparison of the obtained results it indicates that the complete polytropic exponent method is the most accurate. However, the partial polytropic exponent method is of a high applicable value, because it is easy and simple to measure and the obtained results are relatively accurate.

关键词： Polytropic exponent Discharge method Sonic conductance

Joint number and DOA estimation via the eigen-beam mCapon method for closely spaced sources

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Science China(Information Sciences) 2015年第12期58卷 217-219页

作者： GAO yang XU Jia JIA Xin Academy of Equipment School of Information and Electronics Beijing Institute of Technology

Dear editor,The source number and direction of arrival(DOA)estimation are two important subjects in array signal processing[1,2].Many algorithms for estimating these parameters have been proposed in the past

关键词： DOA Joint number and DOA estimation via the eigen-beam mCapon method for closely spaced sources MUSIC

Compound Zhebei granules combined with chemotherapy for the treatment of refractory acute leukemia: a randomized clinical trial

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Journal of Traditional Chinese Medicine 2016年第5期36卷 606-612页

作者： Hou Li yang Shulian yang Wenhua Zhou Yuhong Liu Feng yang Hongyong Sun Weizheng Li Dongyun Xu Yamei Chen Xinyi Department of Oncology and Hematology Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Department of Hematology Langfang Chinese Medicine Hospital Affiliated to Hebei Medical University Department of Hematology First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine Department of Hematology Chinese Medicine Hospital of Zhejiang Province Department of Hematology Xiyuan Hospital Affiliated to China Academy of Chinese Medical Sciences Department of Hematology First Affiliated Hospital of Guangzhou University of Chinese Medicine Department of Hematology First Affiliated Hospital of Heilongjiang University of Chinese Medicine

OBJECTIVE: To observe the effect of compound Zhebei granules(CZBG) with chemotherapy in the treatment of refractory acute ***: In this multicenter, double-blind, placebo-controlled clinical trial, we used a central(online) randomization system to assign 235 patients to two treatment groups. A total of 118 patients received chemotherapy combined with CZBG(4 g,twice daily) and 117 patients received chemotherapy plus placebo. The clinical efficacy was evaluated at the end of one chemotherapeutic ***: In the full analysis set, in which deaths due to disease progression were regarded as inefficacy, the rates of complete remission(CR) and partial remission(CR + PR) were 32.35% and 50.00%,respectively, for the chemotherapy combined with CZBG group, and 23.08% and 35.58%, respectively,for the chemotherapy plus placebo group. There was a statistically significant difference between the two groups according to a ysis set(PPχ2test(P < 0.05). In the per protocol analS), the CR(33.67%),CR+PR(52.04%) response rates for the chemotherapy plus CZBG group were significantly different from the response rates of the control group(CR:24.24% and CR+PR: 37.37%), respectively(P < 0.05).CONCLUSION: CZBG plus chemotherapy can improve the clinical remission rate of refractory acute leukemia after one just one therapeutic cycle.

关键词： Refractory acute leukemia Zhebei granules Chemotherapy remission rate Randomized clinical trial

Designable heteronanocrystals via interface redox reaction

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Nano Research 2023年第4期16卷 5946-5952页

作者： Zhihua Li yang Li Nannan Luo Yuanyuan Qie Dingyi yang Guowei Cao Yuxiang Liu Ying Fu Na Li Wen Hu Min Zhang Rusen yang Bo Tang College of Chemistry Chemical Engineering and Materials ScienceKey Laboratory of Molecular and Nano ProbesMinistry of EducationCollaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of ShandongInstitute of Molecular and Nano ScienceShandong Normal UniversityJinan 250014China School of Advanced Materials and Nanotechnology Xidian UniversityXi'an 710126China

The synergistic interaction of different components in heteronanocrystals induces interfacial phenomena and novel ***,effective technologies to design and fabricate heteronanocrystals with materials on demand are still *** heterostructures in a copper patina are known to form at room temperature and under atmospheric *** redox process of copper tarnish inspired the discovery of a simple strategy to achieve heteronanocrystals that contained elements from group 3-11 and group *** interface redox-induced method is self-regulating at ambient conditions and applicable for metal,semiconductor,and dielectric *** enhanced interface bonding endows the heteronanocrystals with outstanding stability and catalytic performance,while the modular approach enables the design and fabrication of heteronanocrystals with intended materials to meet different purposes.

关键词： heteronanocrystals interface redox-induced synthesis modular approach

Design, synthesis and in vitro evaluation of phenylbenzamidine derivatives as SSRIs

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Chinese Chemical Letters 2007年第7期18卷 814-816页

作者： Jing yang Xiaofang Wang Hui Wen Fang Qin Guang Zhong yang Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Science Beijing 100050 China

A series of phenylbenzamidine analogs were synthesized and tested for their biological activities of inhibiting the reuptake of 5-HT. All of them were new compounds, and their structures were confirmed by 1HNMR, MS an... 详细信息

关键词： SSRIs Phenylbenzamidine XRD

Autonomous Path Planning For Robot Manipulators in Manufacturing Environment

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International Journal of Minerals,Metallurgy and Materials 1998年第2期12卷 104-107页

作者： Wei Wang yang yang Kui Yuan Information Engineering School, UST Beijing, Beijing 100083, China Information Engineering School UST Beijing Beijing 100083 China

A robot intelligent path planning system(RIPPS), which can be used as a robot off-line programming tool was introduced in this paper. C-space modeling and path search are key parts of the system. A fast C-space modeling is presented based on critical collision joint angle(CCJA), C-obstacle boundary can be computed by the feature points of obstacles. A modified A* algorithm is used for path search, although the path is slightly sub-optimal, a tremendous speed increase is achieved. RIPPS runs on a Silicon Graphic 4D/70 workstation, simulation results show that the system is efficient and practical for robot collision-free autonomous path planning in a structured and known environment.

关键词： robot path plalanning A~* algorithm

Dynamic Optimization of Feedforward Automatic Gauge Control Based on Extended Kalman Filter

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Journal of Iron and Steel Research International 2008年第2期15卷 39-42页

作者： yang Bin-hu yang Wei-dong CHEN Lian-gui QU Lei School of Information Engineering University of Science and Technology Beijing Beijing 100083 China

Automatic gauge control is an essentially nonlinear process varying with time delay,and stochastically varying input and process noise always influence the target gauge control *** improve the control capability of feedforward automatic gauge control,Kalman filter was employed to filter the noise signal transferred from one stand to *** linearized matrix that the Kalman filter algorithm needed was concluded;thus,the feedforward automatic gauge control architecture was dynamically *** theoretical analyses and simulation show that the proposed algorithm is reasonable and effective.

关键词： automatic gauge control feedforward control extended Kalman filter

Structure Characterization of Fluorosilicone Oils

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China Petroleum Processing & Petrochemical Technology 2011年第4期13卷 53-57页

作者： yang Jiedan yang He Xu Jing Li Hangquan Beijing University of Chemical Technology Beijing 100029 SINOPEC Research Institute of Petroleum Processing Beijing 100083

Fluorosilicone oil is polysiloxane with alkyl side chains containing fluorine, and because of its excellent thermal oxidation stability, cold flow property and cryogenic property, it can be widely used as a high temperature lubricant in the field of military aerospace industry. Two kinds of fluorosilicone oils, FSiO-a and FSiO-b, were synthesized by different pro- cessing means. FTICR MS was used to collect the information on composition and structure of the two polymers, respec- tively. The test results show that the two fluorosilicone oils have different contents of fluorine-containing chain segments (m/ n value), the maximum distribution of m/n value of FSiO-a oil ranges from 0.22 to 0.25, and that of FSiO-b oil ranges from 0.4 to 0.67. Difference in synthesis techniques makes this discrepancy and affects the quality and thermal stability of the fluoro- silicone oils.

关键词： fluorosilicone oil molecular structure lubricity antioxidant