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水禽3种免疫抑制性病毒多重PCR检测方法的建立与应用

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中国兽医科学 2024年第3期54卷 330-336页

作者：戴银杨雪童雨芹吴希陈瑝儿仲月怡胡晓苗王洁茹殷冬冬尹磊沈学怀潘孝成赵瑞宏安徽省农业科学院畜牧兽医研究所畜禽疫病研究中心安徽合肥230031 畜禽产品安全工程安徽省重点实验室安徽合肥230031

禽流感病毒 H9 亚型(AIV-H9)、鹅圆环病毒(GoCV)和番鸭呼肠孤病毒(MDRV)是水禽常见病原,常发生混合感染且易导致水禽的免疫抑制性疾病。本研究针对 AIV-H9、GoCV 和 MDRV 的基因序列,分别设计特异性引物,通过优化多重 PCR 的反应条件,... 详细信息

禽流感病毒 H9 亚型(AIV-H9)、鹅圆环病毒(GoCV)和番鸭呼肠孤病毒(MDRV)是水禽常见病原,常发生混合感染且易导致水禽的免疫抑制性疾病。本研究针对 AIV-H9、GoCV 和 MDRV 的基因序列,分别设计特异性引物,通过优化多重 PCR 的反应条件,建立了这 3 种病毒的多重 PCR 检测方法,并进行特异性、灵敏度和可重复性验证。结果显示,建立的三重 PCR 检测方法能特异性扩增 AIV-H9、GoCV 和 MDRV的目的片段,与新型鹅星状病毒、鸭瘟病毒、鹅细小病毒、禽腺病毒 4 型无交叉反应;最低检出浓度分别为2.93×10^(4)copies/ μL、2.5×10^(2)copies/ μL、3.0×10^(6)copies/ μL;利用该方法对 30 份临床样品进行检测,MDRV检出率为 6.7%,GoCV 和 AIV-H9 的检出率较高,分别为 36.7%和 26.7%。三重 PCR 与单重 PCR 的符合率为 100%。结果表明,该三重 PCR 检测方法特异、灵敏、稳定性好,能够用于这 3 种病毒的流行病学调查和临床检测。

关键词：禽流感病毒H9亚型鹅圆环病毒番鸭呼肠孤病毒三重PCR

A method to transfer an individual graphene flake to a target position with a precision of sub-micrometer

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Journal of Semiconductors 2017年第4期38卷 103-106页

作者： Yubing Wang Weihong yin Qin Han Xiaohong Yang Han Ye Qianqian Lü dongdong yin State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China

Graphene field-effect transistors have been intensively ***,in order to fabricate devices with more complicated structures,such as the integration with waveguide and other two-dimensional materials,we need to transfer the exfoliated graphene samples to a target *** to the small area of exfoliated graphene and its random distribution,the transfer method requires rather high *** this paper,we systematically study a method to selectively transfer mechanically exfoliated graphene samples to a target position with a precision of *** characterize the doping level of this method,we transfer graphene flakes to pre-patterned metal electrodes,forming graphene field-effect *** hole doping of graphene is calculated to be 2.16×10^12cm^-*** addition,we fabricate a waveguide-integrated multilayer graphene photodetector to demonstrate the viability and accuracy of this method.A photocurrent as high as 0.4 μA is obtained,corresponding to a photoresponsivity of 0.48 mA/*** device performs uniformly in nine illumination cycles.

关键词： graphene field-effect transistor flake

A high-efficiency grating coupler between single-mode fiber and silicon-on-insulator waveguide

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Journal of Semiconductors 2017年第5期38卷 78-81页

作者： Rongrui Liu Yubing Wang dongdong yin Han Ye Xiaohong Yang Qin Han State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of Sciences

We present the design of a diffractive grating structure and get the optimal parameters which can achieve more than 75%coupling efficiency（CE） between single-mode fiber and silicon-on-insulator（SOI） waveguide through 2D finite-different time-domain（FDTD） *** proposed architecture has a uniform structure with no bottom reflection element or silicon *** structure,including grating couplers,adiabatic tapers and interconnection waveguides can be fabricated on the SOI waveguide with only a single electron-beam lithography（ICP） step,which is CMOS-compatible.A relatively high coupling efficiency of 47.2%was obtained at a wavelength of 1562 nm.

关键词： grating coupler coupling efficiency silicon-on-insulator waveguide single-mode fiber integrated optics

3D Braided Material Based on Space Group <i>R</i>3 Symmetry

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Materials Sciences and Applications 2013年第12期4卷 773-779页

作者： Wensuo Ma dongdong yin School of Mechatronics Engineering Henan University of Science and Technology Luoyang China

A unit cell geometrical structure was found with the use of symmetry operations corresponding to the point group C3. Based on the symmetry of space group R3, a 3D braided geometrical structure was obtained by transforming the unit-cell. The features corresponding to this braided structure were studied. The fiber volume percentage and variational tendencies of the material were predicted by establishing a geometric model.

关键词： Point Group C3 Space Group R3 3D Braided Geometrical Structure

Astronomical influence of the development of Paleogene thin coal seam groups in offshore Lacustrine basins:A case study of the ZhuⅠDepression's Enping Formation located in the northern South China Sea

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Acta Oceanologica Sinica 2024年第4期43卷 136-150页

作者： Yan Liu Shengbing Huang dongdong Wang Nan Li Yuting yin ying Chen Zengxue Li College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China CNOOC Research Institute Co. Ltd.Beijing 100028China School of Geography Earth and Environmental SciencesUniversity of BirminghamBirmingham B152TTUK

The development of the Paleogene coal seams in China's offshore basin areas generally had the characteristics of coal measures with large thicknesses,large numbers of coal seams,thin single coal seams,poor stability,scattered vertical distribution,and a wide distribution *** study selected the Enping Formation of the ZhuⅠDepression in the northern section of the South China Sea as an example to determine the macro-control factors of the development of the Paleogene coal seam *** analysis was carried out on the influencing effects and patterns of the astronomical cycles related to the development of the thin coal seam groups in the region.A floating astronomical time scale of the Enping Formation was established,and the sedimentary time limit of the Enping Formation was determined to be approximately 6.15 Ma±.In addition,the cyclostratigraphy analysis results of the natural gamma-ray data of Well XJ in the Enping Formation of the Xijiang Sag revealed that the development of the thin coal seams had probably been affected by short eccentricity and precession *** formation process of coal seams was determined to have been affected by high seasonal contrast,precipitation,and *** the periods with high values of short eccentricity,the seasonal contrasts tended to be *** those periods,fluctuations in the precession controls resulted in periodic volume changes in precipitation and insolation of the region,resulting in the development of thin coal *** was also found that the periods with low precession were the most conducive to coal seam *** that basis,combined with such factors as sedimentary environmental conditions conducive to the development of thin coal seam groups,this study established a theoretical model of the comprehensive influences of short eccentricity and precession on the development and distribution of Paleogene thin coal seam groups in offshore lacustrine *** patterns of the Paleogene astrono

关键词： Paleogene Pearl(Zhujiang)River Mouth Basin coal seam development astronomical cycles

Highly selective hydrogenation of furfural to tetrahydrofurfuryl alcohol over MIL-101(Cr)-NH_2 supported Pd catalyst at low temperature

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Chinese Journal of Catalysis 2018年第2期39卷 319-326页

作者： dongdong yin Hangxing Ren Chuang Li Jinxuan Liu Changhai Liang Laboratory of Advanced Materials and Catalytic Engineering Dalian University of Technology Dalian 116024 Liaoning China Purification Equipment Research Institute of CSIC Handan 056027 Hebei China State Key Laboratory of Fine Chemicals Institute of Artificial Photosynthesis Dalian University of Technology Dalian 116024 Liaoning China

An efficient heterogeneous catalyst,Pd@MIL‐101(Cr)‐NH2,is prepared through a direct pathway of anionic exchange followed by hydrogen reduction with amino‐containing MIL‐101as the host *** composite is thermally stable up to350°C and the Pd nanoparticles uniformly disperse on the matal organic framework(MOF)support,which are attributed to the presence of the amino groups in the frameworks of MIL‐101(Cr)‐*** selective hydrogenation of biomass‐based furfural to tetrahydrofurfuryl alcohol is investigated by using this multifunctional catalyst Pd@MIL‐101(Cr)‐NH2in water media.A complete hydrogenation of furfural is achieved at a low temperature of40°C with the selectivity of tetrahydrofurfuryl alcohol close to100%.The amine‐functionalized MOF improves the hydrogen bonding interactions between the intermediate furfuryl alcohol and the support,which is conducive for the further hydrogenation of furfuryl alcohol to tetrahydrofurfuryl alcohol in good coordination with the metal sites.

关键词： Metal‐organic frameworks Amino functionalization Pd nanoparticle Biomass Selective hydrogenation

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重型商用车节能关键技术分析

内燃机与动力装置 2024年第3期41卷 81-85页

作者：史祥东朱江苏尹东东王福龙宁廷会甄雷内燃机与动力系统全国重点实验室山东潍坊261061 潍柴动力股份有限公司发动机研究院山东潍坊261061

为降低重型商用车燃油消耗量,采用MATLAB软件综合评估柴油机高效清洁燃烧、内燃机余热回收非制冷剂朗肯循环等各项节能技术,结合整车转毂试验测试基准车型、演示样车的燃油消耗量及采用不同节能技术对应的燃油消耗量下降幅度,并对2种车... 详细信息

为降低重型商用车燃油消耗量,采用MATLAB软件综合评估柴油机高效清洁燃烧、内燃机余热回收非制冷剂朗肯循环等各项节能技术,结合整车转毂试验测试基准车型、演示样车的燃油消耗量及采用不同节能技术对应的燃油消耗量下降幅度,并对2种车型的燃油消耗量进行道路测试。结果表明:对于基准车型,MATLAB软件仿真得到重载柴油机高效清洁燃烧技术的综合评估效果最好,单车能源网络优化管理技术、卡车空气动力学减阻技术、卡车低滚轮胎技术的综合评估效果较好;整车转毂试验表明采用节能技术的演示样车在世界重型商用车瞬态循环工况、中国半挂牵引车列车行驶工况的节油率为11.79%和12.46%,低滚阻轮胎技术节油效果最好,燃油消耗量可减少1.42 L/100 km;采用节能技术优化后,综合节油率为12.17%。

关键词：重型商用车关键节能技术整车经济性实车试验

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Formation environment and hydrocarbon potential of the Paleogene Enping Formation coal measures in the ZhuⅠDepression of northern South China Sea

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Acta Oceanologica Sinica 2024年第4期43卷 119-135页

作者： Yuting yin Lei Lan dongdong Wang ying Chen Yan Liu Youchuan Li Zengxue Li Jiamin Liu College of Earth Science and Engineering Shandong University of Science and TechnologyQingdaoShandong 266590China China National Offshore Oil Corporation Research Institute Co. Ltd.Beijing 100028China School of Geography Earth and Environmental SciencesUniversity of BirminghamBirminghamB152TTUK

The coal-measure source rock in the Chinese sea area plays a significant role as a hydrocarbon source rock,with its genetic environment,development and distribution,and hydrocarbon generation potential serving as essential factors for the exploration of coal-type oil and gas *** study focuses on the coal-measure source rock of the Paleogene Enping Formation in the ZhuⅠDepression,located in the northern South China *** main geological insights obtained are as *** coal measures of the Enping Formation are developed in a warm and wet tropical-subtropical *** development environment of the coal-measure source rock in the Enping Formation includes the braided river delta upper plain peat swamp,characterized by dry forest swamp coal facies with relatively thick coal seams and a small number of *** braided river delta lower plain swamp-interdistributary bay of braided river delta front represents a forest edge-wetland herbaceous swamp coal facies with numerous layers of thin coal seams and poor *** shore swamp corresponds to an open water swamp coal facies with multiple layers of thin coal seams and poor *** organic matter abundance in the braided river delta upper plain is the highest,followed by the braided river delta lower plain-braided river delta front,and the shore-shallow *** organic matter type is predominantly typeⅡ*** evolution analysis suggests that the organic matter has progressed into a substantial oil generation *** hydrocarbon generation potential of the coal-measure source rock in the Enping Formation is the highest in the braided river delta upper plain,followed by the braided river delta lower plain-braided river delta front and the shore-shallow ***,this study proposes three organic facies in the coal-measure source rock of the Enping Formation:upper-plain swamp-dry forest swamp facies,lower plain-interdistributary bay-forest-herbaceous swamp facies,and lake swamp-herbaceo

关键词： coal-measure source rock Paleogene genetic environment hydrocarbon generation characteristic ZhuⅠDepression

One-step synthesis of Pt@ZIF-8 catalyst for the selective hydrogenation of 1,4-butynediol to 1,4-butenediol

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Chinese Journal of Catalysis 2016年第9期37卷 1555-1561页

作者： Chuang Li Mingming Zhang Xin Di dongdong yin Wenzhen Li Changhai Liang Laboratory of Advanced Materials and Catalytic Engineering School of Chemical Engineering Dalian University of Technology Dalian 116024 Liaoning China DCBE Biorenewables Research Laboratory Iowa State University Iowa 50011 USA

A catalyst consisting of platinum nanoparticles on a ZIF-8 support（Pt@ZIF-8） was synthesized in a straightforward one-step procedure,by adding a nanostructured platinum sol during the formation of ZIF-8 at room ***@ZIF-8 was highly porous and well *** Pt nanoparticles were well dispersed within the ZIF-8 *** the hydrogenation of 1,4-butynediol,Pt@ZIF-8 exhibited high activity,excellent selectivity for 1,4-butenediol of greater than 94%,and *** Pt@ZIF-8 catalyst did not require further *** favorable catalytic performance was attributed primarily to the modification of the ZIF-8 support by the platinum nanoparticles.

关键词： Platinum sol ZIF-8 One-step synthesis 1 4-Butynediol Selective hydrogenation