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Stability analysis of unsaturated soil slope during rainfall infiltration using coupled liquid-gas-solid three-phase model

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Water Science and Engineering 2016年第3期9卷 183-194页

作者： dong-mei sun Xiao-min Li Ping Feng Yong-ge Zang State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin University

Generally, most soil slope failures are induced by rainfall infiltration, a process that involves interactions between the liquid phase, gas phase,and solid skeleton in an unsaturated soil slope. In this study, a loosely coupled liquid-gas-solid three-phase model, linking two numerical codes,TOUGH2/EOS3, which is used for water-air two-phase flow analysis, and FLAC^(3D), which is used for mechanical analysis, was established. The model was validated through a documented water drainage experiment over a sandy column and a comparison of the results with measured data and simulated results from other researchers. The proposed model was used to investigate the features of water-air two-phase flow and stress fields in an unsaturated soil slope during rainfall infiltration. The slope stability analysis was then performed based on the simulated water-air two-phase seepage and stress fields on a given slip surface. The results show that the safety factor for the given slip surface decreases first, then increases, and later decreases until the rainfall stops. Subsequently, a sudden rise occurs. After that, the safety factor decreases continually and reaches its lowest value, and then increases slowly to a steady value. The lowest value does not occur when the rainfall stops, indicating a delayed effect of the safety factor. The variations of the safety factor for the given slip surface are therefore caused by a combination of pore-air pressure, matric suction, normal stress, and net normal stress.

关键词： Coupled liquid-gas-solid three-phase model Pore-air pressure Unsaturated soil slope stability Rainfall infiltration

Effects of Sirt1 on proliferation,migration,and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease

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Asian Pacific Journal of Tropical Biomedicine 2021年第10期11卷 429-439页

作者： dong-mei sun Jin-Jian Yao Zhan-Ling dong Jin Qian Qi-Feng Huang Yuan-Tian sun Xiao-Ran Liu The First Affiliated Hospital of Hainan Medical University HaikouChina Emergency Center of Hainan General Hospital Affiliated to Hainan Medical University HaikouChina Hainan Medical University HaikouChina Shandong Heze Mudan People's Hospital HezeChina

Objective:To explore the effect of Sirt1 on the function of endothelial progenitor cells(EPCs)in rats with chronic obstructive pulmonary disease(COPD).Methods:A rat COPD model was established via smoking and endotoxin administration for three *** peripheral circulating EPCs were isolated by gradient centrifugation,and their functions,cell cycle distribution,apoptosis,and Sirt1 expression were *** function changes of EPCs in the presence or absence of Sirt1 agonist and inhibitor were estimated;meanwhile,the expressions of Sirt1,FOXO3a,NF-κB,and p53 were also ***:The proliferation,adhesion,and migration of EPCs decreased while the apoptosis rate was increased in the COPD *** expression of Sirt1 protein in EPCs of the COPD group was significantly lower than that in the control group(P<0.01).The overexpression of the Sirt1 gene using a gene transfection technique or Sirt1 agonists(SRT1720)improved the proliferation,migration,and adhesion,and decreased the apoptosis of ***,Sirt1 inhibitor(EX527)decreased EPC functions in the COPD *** effect of Sirt1 expression on EPC function may be related to reduction of FOXO3a and increase of NF-κB and p53 ***:Increased expression of Sirt1 can improve the proliferation and migration of EPCs and reduce their apoptosis in COPD *** change may be related to FOXO3a,NF-κB,and p53 signaling pathways.

关键词： Chronic obstructive pulmonary disease Endothelial progenitor cells Sirt1 Proliferation Migration Adhesion Apoptosis FOXO3a NF-κB p53 Rat

Midkine ameliorates LPS-induced apoptosis of airway smooth muscle cells via the Notch2 pathway

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Asian Pacific Journal of Tropical Biomedicine 2022年第12期12卷 512-519页

作者： Qi-Feng Huang Bo Wang Yu-Qing Weng Tang Deng Li-Hua Li Jin Qian Qi Li Kai-Wen Lin dong-mei sun Shuang-Qin Xu Hang-Fei Wang Xin-Xin Wu Yuan-Tian sun Xiao-Ran Liu Emergency Department The First Affiliated Hospital of Hainan Medical UniversityHaikou 570102China Jiangdong Health Center Haikou 571126China Department of Pulmonary and Critical Care Medicines Zhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University)Zhuhai 519050China College of Pharmacy Hainan Medical UniversityHaikou 570100China Shandong Heze Mudan People’s Hospital Heze 274007China Emergency and Trauma College Hainan Medical UniversityHaikou 570100China College of Basic Medicine and Life Sciences Hainan Medical UniversityHaikou 570100China Key Laboratory of Emergency and Trauma of Ministry of Education Haikou 570100China

Objective:To evaluate the effect of midkine on lipopolysaccharide(LPS)-induced airway smooth muscle cells(ASMCs).Methods:LPS-stimulated acute lung injury model was used to analyze the effect of midkine on ASMCs in *** midkine and midkine siRNA were used to investigate the role of Notch2 signaling *** proliferation was assessed using Cell Counting Kit-8 ***,apoptosis was measured by flow cytometry and protein and mRNA expression of midkine and Notch2 was assessed by Western blotting and qPCR,*** analysis was also ***:LPS increased the mRNA and protein expression of midkine and *** silencing reduced LPS-induced midkine and Notch2 *** addition,midkine silencing further reduced the viability and increased apoptosis of ASMCs induced by LPS,which was attenuated by recombinant ***:The midkine/Notch2 signaling pathway plays a regulatory role in ASMC proliferation and apoptosis in airway inflammation.

关键词： Acute lung injury Airway smooth muscle cells Midkine Notch2 Lipopolysaccharide

Research progress of transfering mitochondria application in nanotubes in treatment of acute lung injury sepsis

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Journal of Acute Disease 2017年第6期6卷 241-244页

作者： Jin Qian dong-mei sun Qi-Feng Huang Xiao-Ran Liu Hainan Medical University 3 Xueyuan RdLonghua DistrictHaikou CityHainan ProvinceChina 570000 Emergency Department The First Affiliated Hospital of Hainan Medical UniversityHaikouHainanChina 570100

Acute lung injury (ALI) is a common complication of sepsis with characteristics of acute onset, rapid change in the disease and high mortality. Since current clinical treatment can only alleviate the unfavorable condition to a certain extent but cure, we urgently need to find an effective treatment. Most scholars believe that sepsis-induced ALI is associated with extensive mitochondrial damage. In recent years, a widely studied pluripotent stem cell that is mesenchymal stem cell has been proved to alleviate and treat sepsis-induced ALI by transporting mitochondria via nanotubes in a microtubule-dependent manner. Research progress in this field will be reviewed in this study.

关键词： Mesenchymal stem cells Nanotubes Mitochondria Sepsis Acute lung injury

Magnetic Resonance Three-dimensional Cube Technique in the Measurement of Piglet Femoral Anteversion

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Chinese Medical Journal 2016年第13期129卷 1584-1591页

作者： dong-mei sun Shi-Nong Pan En-Bo Wang Li-Qiang Zheng Wen-Li Guo Xi-Hu Fu Department of Radiology Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China Department of Pediatric Orthopedics Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China Department of Clinical Epidemiology Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China

Background： The accurate measurement of the femoral anteversion （FA） angle is always a topic of much debate in the orthopedic surgery and radiology research. We aimed to explore a new FA measurement method to acquire accurate results without radiation damage using piglet model. Methods： A total of thirty piglets were assigned to two groups based on the age. Bilateral femora were imaged with 3.0-T magnetic resonance （MR） and 64-slice computed tomography （CT） examinations on all piglets. FA was measured on MR-three-dimensional （3D） postprocessing software with a four-step method： initial validation of the femoral condylar axis, validation of the condylar plane, validation of the femoral neck axis, and line-plane angle measurement of FA. After MR and CT examinations, all piglets were sacrificed and their degree of FA was measured using their excised, dried femora. MR, CT, and dried-femur measurement results were analyzed statistically; M R and CT measurements were compared for accuracy against each other and against the gold standard dried femur measurement. Results： In both groups, the mean FA value measured by MR was lower than that measured by CT. A statistically significant difference was observed between CT- and dried-femur measurements but not between MR- and dried-femur measurements. A higher correlation （0.783 vs. 0.408） and a higher consistency （0.863 vs. 0.578） with dried-femur measurement results were seen for MR measurements than CT measurements in the 1 -week age group. However, in the 8-week age group, similar correlations （0.707 vs. 0.669） and consistencies （0.864 vs. 0.82 l ） were observed. Conclusions： Noninvasive MR-3D-Cube reconstruction was able to accurately measure FA in piglets. Particularly in the 1-week age group with a larger proportion of cartilaginous structures, the correlation and consistency between MR- and dried-femur measurement results were higher than those between CT- and dried-femur measurements, suggesting that MR may be a new usef

关键词： Femoral Anteversion Hip Magnetic Resonance Imaging Piglet Three-dimensional Fast Spin Echo Cube

Analysis of the disease spectra in patients seeking emergency medical treatment in Haikou, China

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Journal of Acute Disease 2017年第5期6卷 205-209页

作者： dong-mei sun Qing-Fei Zhong Qian-E Du Jia-Qi Xu Fu-Jiang Liu Xiao-Ran Liu Hainan Medical University 3Xueyuan RdLonghua QuHaikou ShiHainan ShengChina570000 Emergency Department The First Affiliated Hospital of Hainan Medical UniversityHaikouHainanChina570100

Objective: To explore the characteristics of medical emergency disease spectra in Emergency Department of Haikou, China;and to provide clinical data for emergent disease treatment as well as emergency department personnel and equipment management. Methods: A total of 11870 patients (over 16 years old) who visited Emergency Departments of the First Affiliated Hospital of Hainan Medical University, Haikou city from January 1, 2015, through December 31, 2015 were selected. Data on patients' age, sex, diagnosis, treatment duration, and treatment month were analyzed with Microsoft Excel. Results: Peak treatment times were from 18:00 to 24:00. The top four diseases were involved with respiratory, digestive, nervous, and cardiovascular systems, respectively. Respiratory problems peaked from November to February and digestive problems peaked from February to August. Temperatures ranging from >22.5 ℃to 26 ℃ and high humidity exacerbated respiratory and digestive system diseases. Conclusion:The frequency of specific diseases that require emergency room care are affected by the local climate of Hainan Province.

关键词： Respiratory disease Digestive disease Cardiovascular diseases Nervous system disease

Selection of quality markers of Jasminum amplexicaule based on its anti-diarrheal and anti-inflammatory activities: Effect-target affiliation-traceability-pharmacokinetics strategy

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Chinese Herbal Medicines 2019年第4期11卷 379-386页

作者： Ai-li Xu Bu-ming Liu dong-mei sun Xiao-li Bi Da-ke Cai Zhao Chen Xue-jun Huang Jie-yi Jiang Ping Ding School of Traditional Chinese Medicine Guangzhou University of Chinese MedicineGuangzhou 510006China Guangdong Province Engineering Technology Research Institute of TCM Guangzhou 510095China Guangdong Second TCM Hospital Guangzhou University of Chinese MedicineGuangzhou 510095China Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine Guangzhou 510095China Guangxi Key Laboratory for TCM Quality Control Research Guangxi Institute of Chinese Medicine and Pharmaceutical ScienceNanning 530001China

Objective:To investigate therapeutic mechanism in Jasminum amplexicaule(Oleaceae)and verify its main active component as quality control markers Methods:Established mouse models of diarrhea,intestinal angina,and inflammation were firstly used to select herb fractions with optimum efficacy,followed by an in vitro experiment to determine key targets associated with effects of *** *** selected fractions were isolated and purified,its components were identified,and the obtained compounds were verified for their effects on NF-κB and i ***,effective compounds were administered to rats,their plasma pharmacokinetic parameters were calculated,and quality markers(QMs)reflecting therapeutic activities of *** were ***:Trichloromethane and ethyl acetate fractions had significant anti-diarrheal,anti-inflammatory,and analgesic *** trichloromethane fraction also reduced BDNF,p38 MAPK,p-p38 MAPK,NF-κB p65,and p-NF-κB p65 levels in the ileum in a rhubarb-induced diarrhea mouse ***,it inhibited LPS-induced NF-κB transcription and nitric oxide(NO)production in RAW264.7 macrophages,which suppressed i NOS ***,the trichloromethane fraction was further *** candidate selection identified 17 compounds,and results of in-vitro therapeutic validation indicated that methyl caffeate and isochlorogenic acid B had the strongest anti-diarrheal,anti-inflammatory,and analgesic *** being validated by a UHPLC–MS-MS method,concentrations of these target compounds were accurately determined in the rat plasma and pharmacokinetic parameters were ***,tmax,and t1/2 were respectively 575.35 ng/mL(2.963 nmol/mL),0.5 h,and 0.45 h for methyl caffeate and 262.03 ng/m L(0.5034 nmol/mL),0.25 h,and 2.03 h for isochlorogenic acid *** these candidate compounds exhibited favorable pharmacokinetics,they were considered as QMs of ***:The present study accurately and

关键词： isochlorogenic acid B Jasminum amplexicaule Buch.-Ham.(Oleaceae) methyl caffeate NF-κB pharmacokinetics quality markers

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying sun Haoyan Li dongqing sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Plasmonic tandem heterojunctions enable high-efficiency charge transfer for broad spectrum photocatalytic hydrogen production

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Journal of Energy Chemistry 2025年第1期100卷 710-720页

作者： Wenxuan Chen Xiu-Qing Qiao Guo Hui Bojing sun dongfang Hou meidi Wang Xueqian Wu Tao Wu dong-Sheng Li College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion Materials China Three Gorges University Hubei Three Gorges Laboratory College of Chemistry and Materials Science and Guangdong Provincial Key Laboratory of Supramolecular Coordination Chemistry Jinan University

Rational engineering of semiconductor photocatalysts for efficient hydrogen production is of great significance but still challenging,primarily due to the limitations in charge transfer ***,a fascinating plasmonic tandem heterojunction with the hc-CdS/Mo2C@C heterostructure is aimfully prepared for effectively promoting the charge separation kinetics of the CdS photocatalyst via the synergistic strategy of phase junction,Schottky junction,and photothermal *** difference in atomic configuration between cubic-CdS （c-CdS） and hexagonal-CdS （h-CdS） leads to effective charge separation through a typical Ⅱ charge transfer mechanism,and plasmonic Schottky junction further extracts the electrons in the hc-CdS phase junction to realize gradient charge ***,the photothermal effect of Mo2C@C helps to expand the light absorption,accelerate charge transfer kinetics,and reduce the hydrogen evolution energy *** carbon layer provides a fast channel for charge transfer and protects the photocatalyst from *** a result,the optimized hc-CMC photocatalyst exhibits a significantly high photocatalytic H2production activity of 28.63 mmol/g/h and apparent quantum efficiency of 61.8%,surpassing most of the reported *** study provides a feasible strategy to enhance the charge transfer kinetics and photocatalytic activity of CdS by constructing plasmonic tandem heterogeneous junctions.

关键词： Heterojunctions LSPR Photocatalyst Phase junction Schottky junction Photothermal effect