Midkine ameliorates LPS-induced apoptosis of airway smooth muscle cells via the Notch2 pathway

作     者：Qi-Feng Huang Bo Wang Yu-Qing Weng Tang Deng Li-Hua Li Jin Qian Qi Li Kai-Wen Lin Dong-Mei Sun Shuang-Qin Xu Hang-Fei Wang Xin-Xin Wu Yuan-Tian Sun Xiao-Ran Liu Qi-Feng Huang;Bo Wang;Yu-Qing Weng;Tang Deng;Li-Hua Li;Jin Qian;Qi Li;Kai-Wen Lin;Dong-Mei Sun;Shuang-Qin Xu;Hang-Fei Wang;Xin-Xin Wu;Yuan-Tian Sun;Xiao-Ran Liu

作者机构：Emergency DepartmentThe First Affiliated Hospital of Hainan Medical UniversityHaikou 570102China Jiangdong Health CenterHaikou 571126China Department of Pulmonary and Critical Care MedicinesZhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University)Zhuhai 519050China College of PharmacyHainan Medical UniversityHaikou 570100China Shandong Heze Mudan People’s HospitalHeze 274007China Emergency and Trauma CollegeHainan Medical UniversityHaikou 570100China College of Basic Medicine and Life SciencesHainan Medical UniversityHaikou 570100China Key Laboratory of Emergency and Trauma of Ministry of EducationHaikou 570100China 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2022年第12卷第12期

页      面：512-519页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(NO.81660011,81960351) Hainan Provincial Social Development Foundation(NO.ZDYFXGFY2020004) Hainan Provincial Medical and Health Research Project(NO.22A200036) 

主　　题：Acute lung injury Airway smooth muscle cells Midkine Notch2 Lipopolysaccharide 

摘      要：Objective:To evaluate the effect of midkine on lipopolysaccharide(LPS)-induced airway smooth muscle cells(ASMCs).Methods:LPS-stimulated acute lung injury model was used to analyze the effect of midkine on ASMCs in *** midkine and midkine siRNA were used to investigate the role of Notch2 signaling *** proliferation was assessed using Cell Counting Kit-8 ***,apoptosis was measured by flow cytometry and protein and mRNA expression of midkine and Notch2 was assessed by Western blotting and qPCR,*** analysis was also ***:LPS increased the mRNA and protein expression of midkine and *** silencing reduced LPS-induced midkine and Notch2 *** addition,midkine silencing further reduced the viability and increased apoptosis of ASMCs induced by LPS,which was attenuated by recombinant ***:The midkine/Notch2 signaling pathway plays a regulatory role in ASMC proliferation and apoptosis in airway inflammation.