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Aspergillus pneumonia in renal transplant recipients

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Chinese Medical Journal 2008年第9期121卷 791-794页

作者： ZHANG xiao-dong HU xiao-peng yin Hang WANG Wei ZHANG Xin MA Lin-lin WANG Yong Kidney Transplantation Program Department of Urology Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

Background Filamentous fungal infections are associated with a high morbidity and mortality in solid organ transplants The present study aimed to investigate the aspergillus pneumonia in renal transplant recipients, and its diagnosis as well as treatment. Methods Approximately 2000 cases of renal transplants were retrospectively studied and we focused on cases hospitalized during August 1, 2005 and February 1, 2007, as the study period. The clinical database and electronic records were analyzed. Recently published literature was reviewed. Results There was more diabetes and hypertension in the infected group than in the non-infected group （86% vs 62% and 57% vs 39%, respectively）. Eighty-six percent of recipients from the infected group had delayed graft function. Seven cases with aspergillus pneumonia were identified based on either fungal culture or radiology. Of the 7 cases, 4 died in a few days after diagnosis. Liposomal amphotericin B was used as a first-line therapy. Conclusions Incidences of fungal infection are increasing among renal transplant recipients. Early diagnosis and treatment are critical steps in curing aspergillosis.

关键词： aspergillosis pneumonia, renal transplantation diagnosis treatment

家蝇金属硫蛋白基因的克隆、原核表达及活性检测

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昆虫学报 2010年第4期53卷 379-384页

作者：张迪任国栋唐婷董晓寅柳峰松河北大学生命科学学院河北保定071002

金属硫蛋白是一类普遍存在于生物体内、富含半胱氨酸的小分子蛋白,能螯合多种金属离子。本研究根据EST序列信息,利用RACE技术克隆到1条家蝇Musca domestica金属硫蛋白基因MdMtn(GenBank登录号为GU289398)。序列分析表明,MdMtncDNA全长40... 详细信息

金属硫蛋白是一类普遍存在于生物体内、富含半胱氨酸的小分子蛋白,能螯合多种金属离子。本研究根据EST序列信息,利用RACE技术克隆到1条家蝇Musca domestica金属硫蛋白基因MdMtn(GenBank登录号为GU289398)。序列分析表明,MdMtncDNA全长408bp,包含1个123bp的开放阅读框,编码40个氨基酸残基,其中半胱氨酸残基10个,呈-C-X-C-方式排列。此蛋白理论分子量为3.8kD,等电点为8.78。为了解家蝇金属硫蛋白对重金属的结合活性,构建了pET-DsbA-MT表达载体,并转化Escherichia coliBL21(DE3)宿主菌进行融合表达。研究发现MT重组菌对重金属镉的耐受性得到了明显加强,提示MdMtn基因可能在家蝇适应重金属环境中起到积极作用。

关键词：家蝇金属硫蛋白基因克隆原核表达蛋白活性

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin yin Yalan Xu Jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li yin Wang Peifeng Li Hai Zhu dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

硫氰酸生成酶样基因在家蝇高温和重金属镉应激中的作用

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四川动物 2012年第4期31卷 558-563页

作者：柳峰松李娟唐婷张迪董晓寅河北大学生命科学学院河北保定071002

硫氰酸生成酶同源结构域普遍存在于古细菌、真细菌以及真核生物的多种蛋白分子内,这些蛋白构成一个功能多样的硫氰酸生成酶超家族。本研究根据家蝇转录组序列信息,分拣到一条硫氰酸生成酶样基因的EST序列,经分析发现其编码蛋白C末端含... 详细信息

硫氰酸生成酶同源结构域普遍存在于古细菌、真细菌以及真核生物的多种蛋白分子内,这些蛋白构成一个功能多样的硫氰酸生成酶超家族。本研究根据家蝇转录组序列信息,分拣到一条硫氰酸生成酶样基因的EST序列,经分析发现其编码蛋白C末端含有典型的硫氰酸生成酶同源结构域,属于硫氰酸生成酶超家族,将其命名为家蝇硫氰酸生成酶样基因(Md-Rhodlike)。应用实时定量RT-PCR技术,分析了Md-Rhodlike在热激、镉胁迫条件下的表达量变化。Md-Rhodlike的mRNA水平在高温和镉处理后上调,提示该基因可能参与家蝇对高温及镉金属等环境胁迫因子的耐受,是一种新的家蝇应激相关蛋白。

关键词：家蝇硫氰酸生成酶热激重金属镉应激反应

家蝇半胱氨酸蛋白酶抑制剂基因MdCPI的克隆、表达及活性检测

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昆虫学报 2010年第6期53卷 611-617页

作者：柳峰松董晓寅张瑞英唐婷河北大学生命科学学院河北保定071002

半胱氨酸蛋白酶抑制剂是具有抑制半胱氨酸蛋白酶活性的一类蛋白超家族。本研究根据EST序列信息,通过RACE技术克隆得到1条家蝇Musca domestica半胱氨酸蛋白酶抑制剂基因MdCPI,该基因含有1个357bp开放阅读框,编码118个氨基酸残基,推导的多... 详细信息

半胱氨酸蛋白酶抑制剂是具有抑制半胱氨酸蛋白酶活性的一类蛋白超家族。本研究根据EST序列信息,通过RACE技术克隆得到1条家蝇Musca domestica半胱氨酸蛋白酶抑制剂基因MdCPI,该基因含有1个357bp开放阅读框,编码118个氨基酸残基,推导的多肽N端17个氨基酸残基为信号肽序列。同源分析表明,MdCPI氨基酸序列与红尾肉蝇Sarcophaga crassipalpis的CPI相似性最高(identity=51%)。以邻接法(NJ)构建的系统树表明,家蝇与其他双翅目昆虫CPI起源于共同的祖先,属于I25A型蛋白家族。为了解家蝇CPI对半胱氨酸蛋白酶的抑制活性,构建pET-17b-MdCPI表达载体,并转入大肠杆菌Escherichia coli BL21(DE3)进行重组表达。研究发现1μg重组家蝇CPI能够抑制约14μg木瓜蛋白酶的水解活性。结果表明MdCPI确属CPI家族,可能同其他家族成员具有相似的功能,参与免疫及生理调控。这些结果为研究MdCPI在家蝇体内作用机制奠定了基础。

关键词：家蝇半胱氨酸蛋白酶抑制剂克隆重组表达抑制活性

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中国优秀运动员英语学习现状调查与对策研究

体育科学 2005年第2期25卷 31-34,44页

作者：田慧韩冰许小冬尹红蕾北京体育大学外语系北京100084

采用问卷调查和访谈等方法调查了 8支国家队 181名一线运动员的英语学习现状 ,发现国家队运动员具有强烈的学习英语愿望和正确的英语学习动机 ,但目前国家队只有少数运动员坚持学习英语 ,运动员英语学习方法较单调 ,缺乏良好的英语学习... 详细信息

采用问卷调查和访谈等方法调查了 8支国家队 181名一线运动员的英语学习现状 ,发现国家队运动员具有强烈的学习英语愿望和正确的英语学习动机 ,但目前国家队只有少数运动员坚持学习英语 ,运动员英语学习方法较单调 ,缺乏良好的英语学习环境。据此 ,作者提出提高国家队运动员英语学习效果的 4项对策 :国家队应将英语学习作为运动员全面素质培养的重要内容、选用或编写适合运动员特点的英语教材、突出运动员英语学习重点和营造良好的英语学习环境。

关键词：优秀运动员英语现状对策中国

Induced pluripotent stem cell-related approaches to generate dopaminergic neurons for Parkinson's disease

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Neural Regeneration Research 2025年第11期20卷 3193-3206页

作者： Ling-xiao Yi Hui Ren Woon Genevieve Saw Li Zeng Eng King Tan Zhi dong Zhou National Neuroscience Institute Singapore Nanyang Technology University Singapore Department of Neurology Singapore General HospitalSingapore Signature Research Program in Neuroscience and Behavioral Disorders Duke-NUS Medical SchoolSingapore

The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson's disease,the second most common human neurodegenerative *** the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson's disease is still unclear,the advancement of stem cell approaches has shown promise for Parkinson's disease research and *** induced pluripotent stem cells have been commonly used to generate dopaminergic neurons,which has provided valuable insights to improve our understanding of Parkinson's disease pathogenesis and contributed to anti-Parkinson's disease *** current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem *** benefits of induced pluripotent stem cell-based research are *** dopaminergic neuron differentiation protocols from induced pluripotent stem cells are *** emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are ***,limitations,challenges,and future directions of induced pluripotent stem cell–based approaches are analyzed and proposed,which will be significant to the future application of induced pluripotent stem cell-related techniques for Parkinson's disease.

关键词： dopaminergic neurons induced pluripotent stem cells Parkinson's disease stem cell approaches

Reduced mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor contributes to neurodegeneration in a model of spinal and bulbar muscular atrophy pathology

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Neural Regeneration Research 2025年第9期20卷 2655-2666页

作者： Yiyang Qin Wenzhen Zhu Tingting Guo Yiran Zhang Tingting Xing Peng yin Shihua Li xiao-Jiang Li Su Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong–HongKong–Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina

Spinal and bulbar muscular atrophy is a neurodegenerative disease caused by extended CAG trinucleotide repeats in the androgen receptor gene,which encodes a ligand-dependent transcription facto *** mutant androgen receptor protein,characterized by polyglutamine expansion,is prone to misfolding and forms aggregates in both the nucleus and cytoplasm in the brain in spinal and bulbar muscular atrophy *** aggregates alter protein-protein interactions and compromise transcriptional *** this study,we reported that in both cultured N2a cells and mouse brain,mutant androgen receptor with polyglutamine expansion causes reduced expression of mesencephalic astrocyte-de rived neurotrophic *** n of mesencephalic astrocyte-derived neurotrophic factor amelio rated the neurotoxicity of mutant androgen receptor through the inhibition of mutant androgen receptor *** down endogenous mesencephalic astrocyte-derived neurotrophic factor in the mouse brain exacerbated neuronal damage and mutant androgen receptor *** findings suggest that inhibition of mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor is a potential mechanism underlying neurodegeneration in spinal and bulbar muscular atrophy.

关键词： androgen receptor mesencephalic astrocyte-derived neurotrophic factor mouse model neurodegeneration neuronal loss neurotrophic factor polyglutamine disease protein misfolding spinal and bulbar muscular atrophy transcription factor

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin dong Bin Duan Feiyang Xing Jun Wu Minmin xiao Fangqing Zhao Jing-dong J.Han Qi Liu xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening