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Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1768-1784页

作者： Deok Yun Kim Byeonghwi Lim Jun-Mo Kim dong yong kil Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-do 17546Republic of Korea

Background:Heat stress(HS)is one of the most important threats for the current poultry ***,many efforts have been made to ameliorate the adverse effect of HS on poultry production;however,physiological and molecular mechanisms pertaining to HS are still limited in ***,the objective of the current study was to investigate functional alterations based on individual and integrated transcriptomes in the liver and jejunal mucosa tissues of broiler chickens exposed to HS ***:Broiler chickens exposed to HS showed decreased growth performance and increased corticosterone concentrations in the *** the transcriptome analysis,the number of differentially expressed genes(DEGs)were identified in the liver and jejunal mucosa by HS *** the liver,genes related to amino acid oxidation,tryptophan metabolism,lipid metabolism,oxidative phosphorylation,and immune responses were altered by HS,which support the reason why heat-stressed poultry had decreased productive *** the jejunal mucosa,genes related to defense systems,glutathione metabolism,detoxification of xenobiotics,and immune responses were differently expressed by HS *** integrated transcriptome analysis with DEGs found in the liver and jejunal mucosa showed a considerable connectivity between the core nodes in the constructed networks,which includes glutathione metabolism,xenobiotic metabolism,carbon metabolism,and several amino acid ***:The core network analysis may indicate that increased requirement of energy and amino acids in the jejunal mucosa of broiler chickens exposed to HS conditions is likely compromised by increased oxidation and synthesis of amino acids in the ***,our results may provide comprehensive insights for molecular and metabolic alterations of broiler chickens raised under HS conditions,which can aid in the development of the novel strategies to ameliorate the negative effect of HS on poultry

关键词： Broiler chicken Heat stress Jejunal mucosa Liver Transcriptome

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Increasing concentrations of dietary threonine,tryptophan,and glycine improve growth performance and intestinal health with decreasing stress responses in broiler chickens raised under multiple stress conditions

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Animal Nutrition 2024年第3期18卷 145-153页

作者： Hyun Woo Kim Jong Hyuk Kim Gi Ppeum Han dong yong kil Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-do 17546Republic of Korea Department of Animal Science Chungbuk National UniversityCheongju 28644Republic of Korea

The current study aimed to compare the effects of increasing concentrations of dietary threonine(Thr),tryptophan(Trp),and glycine(Gly)on growth performance,stress biomarkers,and intestinal function in broiler chickens under multiple stress *** hundred sixty broiler chickens at 21 d old were randomly allotted to 5 treatments with 8 *** in a positive control(PC)treatment were raised under low stock density(16.9 birds/m^(2) per cage)with recommended environmental conditions,whereas birds in 4 treatments were subjected to multiple stress conditions:a cyclic heat stress of 30±0.3℃ for 10 h and 23±0.2℃ for 14 h per day with high stock density(25.3 birds/m^(2) per cage).A basal diet was assigned to both PC and negative control(NC)*** additional diets were individually formulated to contain double concentrations of digestible Thr,Trp,or Gly+Ser compared with their concentrations in the basal *** experiment lasted for 14 *** showed that NC treatment had less growth performance(P<0.001),jejunal goblet cell counts(P=0.018),and trans-epithelial electrical resistance(TEER;P<0.001),but greater(P=0.026)feather corticosterone(CORT)concentrations than PC *** treatment showed the least(P<0.001)feed conversion ratio(FCR)among treatments under multiple stress ***,Trp,and Gly treatments had less(P=0.026)feather CORT concentrations,but had greater(P<0.001)TEER than NC *** conclusion,increasing concentrations of dietary Thr,Trp,or Gly improve the growth performance and intestinal health in broiler chickens with decreasing stress response under multiple stress conditions.

关键词： Broiler chicken Glycine Multiple stress Threonine Tryptophan

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Supplementation of different fat sources affects growth performance and carcass composition of finishing pigs

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Journal of Animal Science and Biotechnology 2018年第4期9卷 949-956页

作者： Yanhong Liu dong yong kil Victor G.Perez-Mendoza Minho Song James E.Pettigrew Department of Animal Science University of CaliforniaDavisCAUSA Department of Animal Sciences University of IllinoisUrbanaILUSA Department of Animal Nutrition and Physiology Chung-Ang UniversityAnseongSouth Korea Pancosma QuincyILUSA Department of Animal Science and Biotechnology Chungnam National UniversityDaejeonSouth Korea

Background: There are various fat sources with different energy values and fatty acid compositions that may affect growth performance and carcass composition of grow-finishing pigs. A higher net energy was recently reported in choice white grease compared with soybean oil. Therefore, two experiments were conducted to determine whether practical responses confirm that difference between choice white grease and soybean oil, and to extend the observations to other fat ***: In Exp. 1, pigs fed fats had lower(P < 0.05) average daily feed intake in phase II and overall period,greater(P < 0.05) gain:feed in phase I, phase II, and overall period than pigs fed the control diet. Pigs fed fats tended(P = 0.057) to have thicker backfat depth at the last rib than those fed control. Pigs fed 6% fats had greater(P < 0.01) gain:feed in phase II and overall period than pigs fed 3% fats. During phase I, pigs fed choice white grease grew faster(P < 0.05) than pigs fed soybean oil. In Exp. 2, pigs fed dietary fats(soybean oil, choice white grease, animal-vegetable blend, palm oil, or tallow) had greater(P < 0.01) gain:feed in each phase and overall period, greater(P < 0.01) average daily gain in phase I, but lower(P < 0.01) average daily feed intake in phase II an overall than pigs fed the control diets. The choice white grease also increased(P < 0.05) average daily gain during phase I compared with soybean oil. Pigs fed palm oil had thicker(P < 0.05) backfat depth at the 10 thrib than those fed soybean oil, animal-vegetable blend, or ***: Inclusion of 6% dietary fat improved feed efficiency of finishing pigs, while different fats produced different practical results that may be consistent with their different energy values. Results from the early stage indicate that dietary fats with relatively more saturated fatty acids may provide greater energy than those with relatively more unsaturated fatty acids for growing pigs.

关键词： Carcass composition Dietary fats Energy values Finishing pigs Growth performance

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Comparative standardized ileal amino acid digestibility and metabolizable energy contents of main feed ingredients for growing pigs when adding dietary β-mannanase

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Animal Nutrition 2019年第4期5卷 359-365页

作者： Se Min Jeon Abdolreza Hosseindoust Yo Han Choi Min Ju Kim Kwang Yeol Kim Jun Hyung Lee dong yong kil Beob Gyun Kim Byung Jo Chae Department of Animal Resources Science College of Animal Life SciencesKangwon National UniversityChuncheon 24341South Korea Swine Division National Institute of Animal ScienceRural Development AdministrationCheonan 31000South Korea Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-do 17546South Korea Department of Animal Science and Technology Konkuk UniversitySeoul 143-701South Korea

The present study was conducted to test whether the dietary supplementation of β-mannanase affects amino acids(AA) digestibility,metabolizable energy(ME) contents of corn,wheat,soybean meal,distillers dried grains with solubles,and palm kernel meal(PKM),nutrient digestibility,and growth performance of *** Exp.1,22 cannulated pigs were used for 10 dietary treatments including 5 feed ingredients and 2 β-mannanase concentrations(0 and 0.5 g/kg of the diet) in 6 periods in an incomplete Latin square design to determine the AA and energy *** Exp.2,200 growing pigs were randomly allotted to 4 treatments with 2 nutrient levels(high and low) and 2 concentrations of β-mannanase(2×2 factorial arrangement) in 2 phases(phase 1,d 0 to 21;and phase 2,d 22 to 42).In Exp.1,β-mannanase increased the mean standardized ileal digestibility(SID) of AA in all feed *** amount of digestible energy was increased(P <0.05) in β-mannanase-treated *** fed β-mannanase showed a greater(P <0.05) digestibility of gross energy(GE).The feed-to-gain(F:G) ratio was improved(P <0.01) in pigs fed high-nutrient *** fed β-mannanase in the diets had greater(P <0.05) average daily gain and F:*** phase 2,the concentration of fecal ammonia was decreased(P <0.05) in pigs fed β-*** the 2 experiments,it can be concluded that β-mannanase increases the SID of AA,which has to be considered in balancing the rations.

关键词： Growing pigs β-mannanase Standardized ileal amino acids Ammonia Metabolizable energy

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四线法弱发光样品绝对量子产率的测量

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光谱学与光谱分析 2025年第1期45卷 95-100页

作者：郑永丽张晓东周永丰上海交通大学化学与化工学院上海200240 上海珩翼行科技有限公司上海200235

弱发光样品量子产率测量困难,提出了四线法的测量方法,采用荧光光谱仪和积分球附件相结合的测量方式,主要利用衰减片的添加来实现样品荧光发射信号的相对增强,较大程度提高了弱发光样品量子产率测量的准确度。采用该装置测定了标准物质Y... 详细信息

弱发光样品量子产率测量困难,提出了四线法的测量方法,采用荧光光谱仪和积分球附件相结合的测量方式,主要利用衰减片的添加来实现样品荧光发射信号的相对增强,较大程度提高了弱发光样品量子产率测量的准确度。采用该装置测定了标准物质YG晶体的绝对量子产率为42.0%,相对误差0.95%,验证了荧光分光光度计和积分球结合的方式用于绝对量子产率测量的可行性。然后介绍了四线法用于绝对量子产率测量的依据、测量过程及数据处理方法。采用四线法测量了标准样品罗丹明6G和硫酸奎宁的绝对量子产率,结果显示相对误差分别为1.3%和1.1%,远小于5.0%(量子产率允许误差),表明该方法具有较高的准确性。详细探讨了实验条件对四线法绝对量子产率测量结果的影响,发现测试的最佳条件为衰减片放于激发端、衰减后的光源强度达到荧光光谱仪检测器光强线性响应区间的上限。采用不同浓度罗丹明6G溶液模拟弱发光、中强发光以及强发光样品,分别用二线法(常规方法)和四线法测量各溶液的绝对量子产率,将测试结果与文献报导数据对比,发现四线法更适用于弱发光和中强发光样品绝对量子产率的测量,尤其用于弱发光样品测量时,相对误差由二线法的10%降至四线法的0.32%。

关键词：绝对量子产率四线法衰减片弱发光样品标准样品

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Analytical evaluation and experimental validation on dynamic rocking behavior for shallow foundation considering structural response

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Earthquake Engineering and Engineering Vibration 2022年第1期21卷 37-51页

作者： kil-Wan Ko Jeong-Gon Ha dong-Soo Kim Department of Civil and Environmental Engineering UC BerkeleyCalifornia 94720USA Advanced Structures and Seismic Safety Research Division Daejeon 34057Korea Department of Civil and Environmental Engineering KAISTDaejeon 34141Korea

The challenge in the practical application of rocking foundations is the estimation of its performance,particularly the rotation angle,during a strong *** this study,the dynamic rocking behavior for a shallow foundation considering structural response was evaluated through two analytical approaches:the conventional soil-foundation-structure interaction(SFSI)governing equation of a single-degree-of-freedom(SDOF)structure on a rocking shallow foundation,and the Housner rocking model(i.e.,a rocking rigid block on a rigid base).Both approaches were validated with dynamic centrifuge *** test models consisted of a soft soil deposit,a shallow rectangular foundation,and an SDOF structure dominated by a bending behavior.A total of 11 foundation-structure systems and six seismic waves,including recorded earthquake signals and sinusoidal waves,were *** results showed that the conventional SFSI equation well predicted the maximum rotation during strong ***,this method was less accurate regarding the rotational phase information and maximum rotation of the foundation during weak *** the other hand,although the modified Housner′s rocking model required five parameters relevant to a soil-foundation-structure system,it overestimated the maximum rotation of the foundation when compared with the results from dynamic centrifuge tests.

关键词： soil-foundation-structure interaction maximum rotation angle rocking foundation Housner rocking model dynamic centrifuge test

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基于SE注意力多源域对抗网络的射频指纹识别

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计算机科学 2025年第1期52卷 412-419页

作者：苏超然张大龙黄勇董安郑州大学网络空间安全学院郑州450002

射频指纹利用射频前端的硬件特征作为标识符对设备进行识别。针对现有射频指纹识别研究忽略接收机硬件特性的干扰,导致模型在不同接收机设备上泛化性较差的问题,提出一种基于SE(Squeeze-and-Excitation)注意力多源域对抗网络的射频指纹... 详细信息

射频指纹利用射频前端的硬件特征作为标识符对设备进行识别。针对现有射频指纹识别研究忽略接收机硬件特性的干扰,导致模型在不同接收机设备上泛化性较差的问题,提出一种基于SE(Squeeze-and-Excitation)注意力多源域对抗网络的射频指纹识别方法。该方法采用多个源域有标签数据和少量目标域无标签数据进行对抗训练以提取与接收机域无关的特征;融合SE注意力机制增强模型对发送机射频指纹特征的学习能力;结合极少量目标域有标签数据对模型参数进行微调,进一步提高发送机识别性能。在Wisig公开数据集上的实验结果表明:该方法在跨接收机场景下可有效识别发送机设备,平均准确率可达83.1%;加入少量有标签数据微调后平均准确率可进一步提高至93.1%。

关键词：射频指纹识别多源域对抗深度学习物理层安全 SE注意力机制

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Crushing behavior of lightmass structural member

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中国有色金属学会会刊：英文版 2009年第B09期19卷 276-279页

作者： kil-Sung LEE yong-June YANG Woo-Chae HWANG In-Young YANG Department of Mechatronics Chosun University of Science and Technology Department of Advanced Part and Materials Engineering Chosun University Department of Mechanical Design Engineering Chosun University

Carbon fiber reinforced plastic (CFRP) of the advanced composite materials is widely used in lightmass structural materials of air planes, ship and automobiles because of high strength and stiffness. In this study, experimental investigation was performed for each specimen. The square section members consist of aluminum, CFRP and hybrid (aluminum/CFRP) member, and hat-shaped section members consist of CFRP and hybrid (aluminum/CFRP) members specimen. Based on the collapse characteristics of aluminum square section member, the collapse characteristics and energy absorption capability of hat-shaped section members were analyzed. The axial static collapse tests were carried out for each section member. The collapse modes and the energy absorption capability of the members were analyzed. In the lightmass design aspect, the collapse characteristics and energy absorption capability of the members were compared.

关键词：碳纤维增强复合材料碳纤维复合材料能量吸收能力行为构件破碎航空飞机 CFRP

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Numerical study on wave loads and motions of two ships advancing in waves by using three-dimensional translating-pulsating source

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Acta Mechanica Sinica 2013年第4期29卷 494-502页

作者： yong Xu Wen-Cai dong Department of Naval Architecture Naval University of Engineering

A frequency domain analysis method based on the three-dimensional translating-pulsating （3DTP） source Green function is developed to investigate wave loads and free motions of two ships advancing on parallel course in waves. Two experiments are carried out respectively to mea- sure the wave loads and the free motions for a pair of side-by- side arranged ship models advancing with an identical speed in head regular waves. For comparison, each model is also tested alone. Predictions obtained by the present solution are found in favorable agreement with the model tests and are more accurate than the traditional method based on the three dimensional pulsating （3DP） source Green function. Numer- ical resonances and peak shift can be found in the 3DP pre- dictions, which result from the wave energy trapped in the gap between two ships and the extremely inhomogeneous wave load distribution on each hull. However, they can be eliminated by 3DTP, in which the speed affects the free sur- face and most of the wave energy can be escaped from the gap. Both the experiment and the present prediction show that hydrodynamic interaction effects on wave loads and free motions are significant. The present solver may serve as a validated tool to predict wave loads and motions of two ves- sels under replenishment at sea, and may help to evaluate the hydrodynamic interaction effects on the ships safety in replenishment operation.

关键词： Hydrodynamic interaction - Wave loads ~Ship motions ~ Model test ~ Three-dimensional translating-pulsating source ~ Underway replenishment

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Multi-response optimization of Ti-6A1-4V turning operations using Taguchi-based grey relational analysis coupled with kernel principal component analysis

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Advances in Manufacturing 2019年第2期7卷 142-154页

作者： Ning Li yong-Jie Chen dong-dong Kong School of Mechanical Science and Engineering Huazhong University of Science and Technology Wuhan 430074 People's Republic of China

Ti-6A1-4V has a wide range of applications, especially in the aerospace field;however, it is a difficultto- cut material. In order to achieve sustainable machining of Ti?6A1-4V, multiple objectives considering not only economic and technical requirements but also the environmental requirement need to be optimized simultaneously. In this work, the optimization design of process parameters such as type of inserts, feed rate, and depth of cut for Ti-6A1-4V turning under dry condition was investigated experimentally. The major performance indexes chosen to evaluate this sustainable process were radial thrust, cutting power, and coefficient of friction at the toolchip interface. Considering the nonlinearity between the various objectives, grey relational analysis (GRA) was first performed to transform these indexes into the corresponding grey relational coefficients, and then kernel principal component analysis (KPCA) was applied to extract the kernel principal components and determine the corresponding weights which showed their relative importance. Eventually, kernel grey relational grade (KGRG) was proposed as the optimization criterion to identify the optimal combination of process parameters. The results of the range analysis show that the depth of cut has the most significant effect, followed by the feed rate and type of inserts. Confirmation tests clearly show that the modified method combining GRA with KPCA outperforms the traditional GRA method with equal weights and the hybrid method based on GRA and PCA.

关键词： Ti-6A1-4V Taguchi method Grey relational analysis (GRA) Kernel principal component analysis (KPCA) Multi-response optimization

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