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Proteomic identification of cryostress in epididymal spermatozoa

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Journal of Animal Science and Biotechnology 2017年第2期8卷 478-489页

作者： Sung Jae Yoon Md Saidur Rahman Woo Sung Kwon do yeal ryu Yoo Jin Park Myung Geol Pang Department of Animal Science&Technology Chung-Ang UniversityAnseongGyeonggi-Do 456-756Republic of Korea

Background： Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation,thereby reducing sperm quality. We assessed the motility（%）, motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence ***, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis（2-DE） results using two commercial ***： Cryopreservation reduced viability（%）, motility（%）, straight-line velocity（VSL）, average path velocity（VAP）, amplitude of lateral head displacement（ALH）, and capacitated spermatozoa, whereas straightness（STR）and the acrosome reaction increased after cryopreservation（P 3 fold, P ＆lt; 0.05） before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation,and the guanylate cyclase ***： The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.

关键词： Cryopreservation Cryostress Protein Spermatozoa

Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1743-1753页

作者： Won-Ki Pang Shehreen Amjad do-yeal ryu Elikanah Olusayo Adegoke Md Saidur Rahman Yoo-Jin Park Myung-Geol Pang Department of Animal Science&Technology and BET Research Institute Chung-Ang UniversityAnseongGyeonggi-do 17546Republic of Korea

Background:Male infertility is an important issue that causes low production in the animal *** solve the male fertility crisis in the animal industry,the prediction of sperm quality is the most important *** RNA is the potential marker for male fertility *** hypothesized that the expression of functional genes related to fertilization will be the best target for male fertility prediction *** investigate optimum male fertility prediction marker,we compared target genes expression level and a wide range of field data acquired from artificial insemination of boar ***:Among the genes related to acrosomal vesicle exocytosis and sperm–oocyte fusion,equatorin(EQTN),zona pellucida sperm-binding protein 4(ZP4),and sperm acrosome membrane-associated protein 3 exhibited high accuracy(70%,90%,and 70%,respectively)as markers to evaluate male *** of EQTN-ZP4,ZP4-protein unc-13 homolog B,and ZP4-regulating synaptic membrane exocytosis protein 1(RIMS1)showed the highest prediction value,and all these markers are involved in the acrosome ***:The EQTN-ZP4 model was efficient in clustering the high-fertility group and may be useful for selection of animal that has superior fertility in the livestock *** to the EQTN-ZP4 model,the ZP4-RIMS1 model was more efficient in clustering the low-fertility group and may be useful in the diagnosis of male infertility in humans and other *** appointed translational animal model and established biomarker combination can be widely used in various scientific fields such as biomedical science.

关键词： Male fertility Multiple markers Pig model Prediction model Sperm RNA

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获得精细通孔图形的光刻技术改进(英文)

液晶与显示 2006年第5期21卷 447-450页

作者：宋泳锡劉聖烈柳在一張炳鉉李禹奉李貞烈京东方科技集团股份有限公司中央研究院

为了获得更高性能的TFT—LCD面板，在光刻时保证精细的图形成像十分重要，其中，如何制作出尺寸更小的通孔图形是主要的问题之一。本文提供的研究中，我们只简单地改变了一下烘烤工艺，而不需要改变Eop和显影时间，就可以将通孔图形的... 详细信息

为了获得更高性能的TFT—LCD面板，在光刻时保证精细的图形成像十分重要，其中，如何制作出尺寸更小的通孔图形是主要的问题之一。本文提供的研究中，我们只简单地改变了一下烘烤工艺，而不需要改变Eop和显影时间，就可以将通孔图形的尺寸减小20％～25oA。在后续的刻蚀工艺中，通孔的尺寸能显著减小。

关键词： TFT—LCD 光刻通孔图形尺寸

利用CF_4等离子体制作高开口率TFT-LCD(英文)

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液晶与显示 2006年第5期21卷 409-413页

作者：金奉柱崔瑩石劉聖烈張炳鉉柳在一李禹奉李貞烈京东方科技集团股份有限公司中央研究院北京100016

为了获得高的开口率,有必要优化设计参数和工艺容差。通常的过孔刻蚀工艺采用SF6基气体进行刻蚀,但是这种方法在金属和钝化层之间的选择性太小,因此,必须增加过孔的尺寸才行。为了克服上述问题,在本研究中用CF4气体代替SF6气体进行刻蚀... 详细信息

为了获得高的开口率,有必要优化设计参数和工艺容差。通常的过孔刻蚀工艺采用SF6基气体进行刻蚀,但是这种方法在金属和钝化层之间的选择性太小,因此,必须增加过孔的尺寸才行。为了克服上述问题,在本研究中用CF4气体代替SF6气体进行刻蚀,结果在FFS 5 .16(2 .03 in)像素结构中,开口率提高了60 %。

关键词： TFT—LCD 开口率刻蚀 CF4等离子体