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Multiplex PCR for Identification and Detection of Cassava Mosaic Begomoviruses in Togo

Advances in Microbiology 2023年第11期13卷 517-525页

作者： Senya Sakina Allado djodji kossikouma adjata Justin Simon Pita Assion Sétu Mivedor Kodjovi Atassé Dansou-Kodjo Koffi Tozo Laboratoire de Virologie et de Biotechnologies Vé g&eacutetales Ecole Sup&eacuterieure d’Agronomie (ESA) Universit&eacute de Lom&eacute Lom&eacute Togo Laboratory of Plant Virology Universit&eacute F&eacutelix Houphou&eumlt-Boigny P&ocircle Scientifique et d’Innovation Bingerville C&ocircte d’Ivoire Laboratoire de Physiologie et Biotechnologies Vé g&eacutetales Facult&eacute des Sciences Universit&eacute de Lom&eacute Lom&eacute Togo

Cassava mosaic disease (CMD) caused by Cassava Mosaic Begomoviruses (CMBs) is one of the most devastating crop diseases and a major constraint for cassava production. In order to ensure surveillance for epidemic prevention, low-cost diagnostic tools are appropriate for large-scale testing of cassava viruses. Multiplex PCR diagnosis is one approach that can reduce diagnostic costs and delays. A multiplex PCR approach was developed for simultaneous detection of African cassava mosaic virus (ACMV), East African Cassava Mosaic Virus and East African cassava mosaic Cameroon virus (EACMV/CM) in Togo CMD-infected cassava leaves. Three primers pairs were used to target their respective viruses in a single tube PCR. Multiplex PCR detected ACMV, EACMV and EACMV/CM in plant DNA extracts prepared from cassava leaves infected with CMB. The primers amplified 783 bp specific to ACMV, 650 bp specific to EACMV and 560 bp specific to EACMCV/CM in both uniplex and multiplex formats. Multiplex PCR is an excellent tool for the effective control of cassava diseases. .

关键词： Cassava Begomovirus CMD CMB EACMV/CM PCR Multiplex

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Genetic Diversity of Tomato(Solanum lycopersicum L.)Begomovirus in Togo

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Agricultural Sciences 2017年第12期8卷 1402-1414页

作者： Assion Setu Mivedor Justin Simon Pita kossikouma djodji adjata Jerome Duclercq Yawovi Mawuena Dieudonne Gumedzoe Laboratory of Plant Virology and Biotechnology(LVBV):Ecole Superieure d’Agronomie(ESA)/University of Lome LoméTogo Laboratory of Agroecology Ecophysiology and Integrative Biology(AEB)Unit EDYSAN FRE 3498 CNRS/University of Picardie Jules VerneAmiensFrance Universite Felix Houphouet-Boigny Laboratory of Plant VirologyPole Scientifique et d’InnovationBingervilleCote d’Ivoire

Geminiviruses, in particular the members of the genus Begomovirus , are considered to be a major phytosanitary problem for tomato crops production in the world. They are responsible for yield losses of up to 20% to 100%. Regrettably, Togo is not spared from this situation. This work aims to show the genetic diversity of the begomoviruses affecting tomato crops production in Togo and their relationship with other begomoviruses. To achieve these objectives, 307 samples of tomato leaves and wild plant species with typical virus symptoms were collected in the Maritime, Plateaus, Central, Kara and Savannah regions and submitted to PCR analysis. The results revealed the presence of begomovirus in 25.40% of the analyzed samples. The PCR products obtained were submitted to direct sequencing. Phylogenetic analysis of sequences of DNA-A different regions of begomovirus identified in this work with that of other begomoviruses showed a nucleotide identity of 96% respectively for Tomato leaf curl Togo virus-Fontem, Tomato Leaf Curl Togo Virus , Ageratum leaf curl Cameroon Alphasatellite;98% respectively to Tomato leaf curl Nigeria virus , Ageratum leaf curl Cameroon virus , Tomato leaf curl Cameroon virus-Fontem, Ageratum leaf curl Cameroon virus and 99% respectively to Tomato leaf curl Kumasi virus , Pepper yellow vein Mali virus Bazegahot and Pepper yellow vein Mali virus-Ouaga. These results suggest a high degree of genetic diversity of tomato begomoviruses identified in Togo.

关键词： Begomoviruses Direct Sequencing Phylogenetic Relationships Tomato Wild Plants

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Occurrence of Cassava Mosaic Begomovirus New Species and Ageratum Leaf Curl Cameroon Virus on Pepper(Capsicum annuum L.)in Togo

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Agricultural Sciences 2019年第5期10卷 671-682页

作者： Kodjovi Atassé Dansou-Kodjo Assion Sétu Mivedor kossikouma djodji adjata Jérome Duclercq Yawovi Mawuena Dieudonné Gumedzoe Laboratory of Plant Virology and Biotechnology(LVBV) Ecole Supérieure d’Agronomie(ESA)University of LomeLoméTogo Laboratory of Agroecology Ecophysiology and Integrative Biology(AEB)Unit EDYSAN FRE 3498 CNRS/University of Picardie Jules VerneAmiensFrance

Cassava mosaic disease caused by the whitefly-transmitted begomoviruses (family Geminiviridae) is a major threat to cassava (Manihot esculenta Crantz) production, which can be intercropped with other plants such as pepper (Capsicum annuum L.). The aim of this study is to identify cassava begomoviruses on other crops in cassava intercropping systems. Thus, foliar samples showing typical symptoms of virus diseases in cassava intercropping systems were collected from pepper and submitted to PCR analysis and direct sequencing. Three begomovirus species ACMV, EACMV and ALCCMV were identified and characterized in samples. Isolates of these species shared respectively 90% - 93%, 74% and 80% nucleotide identities with begomoviruses. These findings show that cassava begomoviruses can infect other crops and will help in understanding the epidemiology related to whitefly-transmitted begomoviruses in cassava intercropping systems.

关键词： ACMV ALCCMV Begomovirus EACMV Pepper Sequencing

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