Genetic Diversity of Tomato(Solanum lycopersicum L.)Begomovirus in Togo

作     者：Assion Setu Mivedor Justin Simon Pita Kossikouma Djodji Adjata Jerome Duclercq Yawovi Mawuena Dieudonne Gumedzoe 

作者机构：Laboratory of Plant Virology and Biotechnology(LVBV):Ecole Superieure d’Agronomie(ESA)/University of LomeLoméTogo Laboratory of AgroecologyEcophysiology and Integrative Biology(AEB)Unit EDYSAN FRE 3498 CNRS/University of Picardie Jules VerneAmiensFrance Universite Felix Houphouet-BoignyLaboratory of Plant VirologyPole Scientifique et d’InnovationBingervilleCote d’Ivoire 

出 版 物：《Agricultural Sciences》 (农业科学（英文）)

年 卷 期：2017年第8卷第12期

页      面：1402-1414页

学科分类：09[农学] 0904[农学-植物保护] 

基　　金：the West African Agricultural Productivity Program(WAAPP/TOGO)and Bill and Melinda Gates Foundation(BMGF)for supporting this project 

主　　题：Begomoviruses Direct Sequencing Phylogenetic Relationships Tomato Wild Plants 

摘      要：Geminiviruses, in particular the members of the genus Begomovirus , are considered to be a major phytosanitary problem for tomato crops production in the world. They are responsible for yield losses of up to 20% to 100%. Regrettably, Togo is not spared from this situation. This work aims to show the genetic diversity of the begomoviruses affecting tomato crops production in Togo and their relationship with other begomoviruses. To achieve these objectives, 307 samples of tomato leaves and wild plant species with typical virus symptoms were collected in the Maritime, Plateaus, Central, Kara and Savannah regions and submitted to PCR analysis. The results revealed the presence of begomovirus in 25.40% of the analyzed samples. The PCR products obtained were submitted to direct sequencing. Phylogenetic analysis of sequences of DNA-A different regions of begomovirus identified in this work with that of other begomoviruses showed a nucleotide identity of 96% respectively for Tomato leaf curl Togo virus-Fontem, Tomato Leaf Curl Togo Virus , Ageratum leaf curl Cameroon Alphasatellite;98% respectively to Tomato leaf curl Nigeria virus , Ageratum leaf curl Cameroon virus , Tomato leaf curl Cameroon virus-Fontem, Ageratum leaf curl Cameroon virus and 99% respectively to Tomato leaf curl Kumasi virus , Pepper yellow vein Mali virus Bazegahot and Pepper yellow vein Mali virus-Ouaga. These results suggest a high degree of genetic diversity of tomato begomoviruses identified in Togo.