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Snai-1 and Epithelial-mesenchymal Transition-Related Protein Immunoexpression in Canine mammary Carcinomas

Advances in Breast Cancer Research 2014年第4期3卷 111-117页

作者： Breno S.Salgado Rafael m.Rocha Fernando A.Soares Fátima Gartner Noeme S.Rocha Departamento de Patologia Faculdade de Medicina de BotucatuUniversidade Estadual Paulista(UNESP)BotucatuBrazil Laboratório de Patologia Investigativa e Comparada Departamento de Clínica VeterináriaFaculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista(UNESP)BotucatuBrazil Departamento de Anatomia Patológica Hospital A.C.CamargoFundacao Antonio PrudenteSao PauloBrazil Institute of Pathology and molecular Immunology(IPATImUP) OportoPortugal

Epithelial-mesenchymal transition (EmT) is defined as switching of polarized epithelial cells to a migratory fibroblastoid phenotype. EmT is known to be involved in the progression and metastasis of various cancers in humans, but this specific process is still little explored in the veterinary literature. The aim of this research was to evaluate the expression of EmT-related proteins in canine mammary carcinomas (CmCs). The expression of six EmT-related proteins in 94 CmCs of female dogs was evaluated by immunohistochemistry using a tissue array method. Additionally, clinicopathological characteristics were compared with the expression of EmT-related proteins. Loss of epithelial protein and/or acquisition of the expression of mesenchymal proteins were observed in CmCs. Loss of epithelial protein and/or acquisition of the expression of mesenchymal proteins were observed, particularly in tumors with evidence of stromal invasion;however, significance was only observed between the S100A4 and vascular invasion. In addition, Snai-1 nuclear immunoexpression was significantly related to E-cadherin loss. In conclusion, loss of epithelial proteins and/or the acquisition of mesenchymal proteins are associated with EmT and may have an important role in the evaluation of CmC patients. The unique immunoexpression pattern of Snai-1 could help to distinguish between an adenoma and a non-metastatic carcinoma and seems to be related to conversion of myoepithelial cells to a complete mesenchymal-like phenotype. Loss of E-cadherin and cytokeratin and change of immunoexpression pattern of Snai-1, N-cadherin, S100A4 and mmP-2 indicate the occurrence of EmT in canine mammary carcinomas and should result in an en bloc resection or a close follow-up.

关键词： EmT S100A4 Keratin mammary Tumors Dog

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Analysis of Tracheal Secretion in Healthy Horses Undergoing a Vaquejada Simulation Test

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Open Journal of Veterinary medicine 2014年第10期4卷 232-238页

作者： Natália matos Souza Azevedo márlon de Vasconcelos Azevedo Paulo Fernandes de Lima Hélio Cordeiro manso Filho Departamento de medicina Veterinária Universidade Federal Rural de Pernambuco Recife Brazil Departamento de Zootecnia Universidade Federal Rural de Pernambuco Recife Brazil

This study aimed to evaluate the occurrence of EIPH in vaquejada horses via endoscopy and tracheal lavage and citology. We used eight Quarter-mile horses that usually perform at vaquejadas. Clinical examination was carried out before the race. After the race, endoscopy and tracheal lavage with 20 ml of saline via the service channel of the endoscope were performed. The samples were sent to the laboratory for processing and confection of the slides. Of the eight horses (four pull and four helper horses), six had hemosiderophages in their tracheal aspirates, confirming the existence of pulmonary hemorrhage after exercise. No difference was observed between the analyzed parameters between the two groups of vaquejada horses (P > 0.05). Tracheal lavage and endoscopy can be used for evaluating this important disease in animal athletes.

关键词： EIPH Horses Sport Endoscopy

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新型甲基取代的聚芳醚酮醚砜醚酮无规共聚物的合成与表征

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江西师范大学学报（自然科学版） 2005年第3期29卷 189-192页

作者：刘晓玲林淑英罗秋燕王琼霍毅盛寿日江西师范大学化学化工学院教育科学学院江西南昌330027

在无水AlCl3及N,N-二甲基甲酰胺(DmF)存在下,将4,4′-二(4-氯甲酰苯氧基)二苯砜(SPCl)与3-甲基二苯醚(mDPE)和二苯醚(DPE)在1,2-二氯乙烷中进行低温溶液共缩聚反应,合成了一系列聚芳醚酮醚砜醚酮/甲基取代聚芳醚酮醚砜醚酮(PEKESEK/m-PE... 详细信息

在无水AlCl3及N,N-二甲基甲酰胺(DmF)存在下,将4,4′-二(4-氯甲酰苯氧基)二苯砜(SPCl)与3-甲基二苯醚(mDPE)和二苯醚(DPE)在1,2-二氯乙烷中进行低温溶液共缩聚反应,合成了一系列聚芳醚酮醚砜醚酮/甲基取代聚芳醚酮醚砜醚酮(PEKESEK/m-PEKESEK)共聚物.经FT-IR,DSC,TG及WAXD等测试表明共聚物为非晶态结构,具有优异的耐热性能,其玻璃化转变温度(Tg)为160～172℃,且随共聚物甲基取代m-PEKESEK结构单元含量的增加而增加.共聚物在氮气气氛中5%的热失重温度(Td)均在460℃以上,且易溶于氯仿、二氯甲烷及DmF和DmSO等强极性非质子有机溶剂中.

关键词：聚芳醚酮甲基取代合成与表征无规共聚物 N-二甲基甲酰胺无水AlCl3 玻璃化转变温度共缩聚反应非晶态结构二氯乙烷 WAXD 耐热性能失重温度氮气气氛有机溶剂 DmSO 二氯甲烷二苯醚二苯砜苯氧基 DSC K结构 DmF

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Follicular Fluid Cortisol Releasing Hormone (CRH) Levels and Assisted Reproductive Treatment (ART) Outcomes

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Open Journal of Obstetrics and Gynecology 2017年第13期7卷 1271-1281页

作者： L. N. Lim P. R. Supramaniam m. mittal E. A. Linton E. mcVeigh Oxford University Hospitals NHS Foundation Trust John Radcliffe Hospital Oxford UK Nuffield Department of Obstetrics and Gynaecology Women’s Centre University of Oxford John Radcliffe Hospital Oxford UK

Purpose: Can Cortisol Releasing Hormone (CRH) levels in follicular fluid predict outcomes following assisted reproductive treatment (ART) cycles? methods: Prospective cohort study of 50 women undergoing in vitro fertilisation (IVF)/intra-cytoplasmic sperm injection (ICSI) cycles over a two month study period. All patients were treated on the long stimulation protocol;follicular fluid was aspirated and pooled for each patient. The samples were processed appropriately and assayed using CRH radioimmunoassay (ria). Results: This study confirmed that CRH was present in follicular fluid. The average level detected was 173 ± 9 pg/mL (mean ± standard error of mean [SEm]). The data suggests a positive correlation of CRH follicular fluid levels greater than 145 pg/mL with successful ART outcomes. Conclusion: The data indicates a positive correlation between ART outcomes and the presence of follicular fluid CRH levels greater than 145 pg/mL. The results should be interpreted with caution due to the small sample size and pooling of follicular fluid per patient. Furthermore, the pooling of follicular fluid is not representative of CRH levels in an individual follicle, and thus, mature oocyte. This study serves as a reminder to what has previously been hypothesised.

关键词： Cortisol Releasing Hormone (CRH) In Vitro Fertilization (IVF) Intra-Cytoplasmic Sperm Injection (ICSI) Follicles mature Oocytes

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Effects of voluntary imipramine intake via food and water in paradigms of anxiety and depression in naive mice

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Translational Neuroscience and Clinics 2016年第3期2卷 172-182,149页

作者： Joao Pedro Costa-Nunes Anastassia Bakhmet margarida Araújo-Correia Andreia Barbosa Valenca Tatyana Strekalova Harry W.m.Steinbusch School for mental Health and Neuroscience Maastricht University Center for Neuroscience and Cell Biology Universidade de CoimbraFaculdade de MedicinaRua Larga Centro de Biologia Ambiental Faculdade de Ciências da Universidade de Lisboa Department of Human Anatomy Moscow State Medical University Centro de Estudos de Doencas Crónicas(CEDOC) Edifício CEDOCⅡRua Camara Pestana Centro Interdisciplinar de Investigacao em Saúde Animal(CIISA) Faculdade de Medicina VeterináriaAlameda da Universidade Técnica

Objective: We sought to investigate the efficacy of oral dosing in mice with imipramine(7mg/kg/day) via water or in food pellets, and to compare its effects in the paradigms of learned helplessness, locomotion, hedonic state, and anxiety. methods: Water and food consumption were measured to determine daily imipramine dosage in C57BL/6N mice. Next, baseline scores for O-maze, dark/light box, and sucrose tests were measured. mice were then subjected to a 4-week treatment of voluntary ingestion of drinking water or food pellets containing imipramine. Lastly, all groups were subjected to novel cage, open field, O-maze, dark/light box, sucrose test, and forced swim test to assess the effects of the treatment. Results: In na?ve mice, imipramine delivered via food, induced a reduction of total floating and increased latency in the forced swim test, i.e., antidepressant-like effects. No other significant effects were found. Dosing with water did not change behavior in the forced swim, sucrose preference test, anxiety, or locomotor paradigms, but increased exploration in the novel cage. Conclusions: Voluntary ingestion is an effective method of chronic dosing with imipramine in na?ve mice. Delivery of imipramine with food pellets elicits antidepressant-like effects in the forced swim test, with no effects on anxiety, locomotion, or preference behaviors. In contrast, no such effects were observed with treatment via drinking water, suggesting that a higher dose may be required. Our work argues for a broader use of oral delivery using food-treated pellets, in small rodent models of pre-clinical depression. It may substantially improve animal welfare and overcome potential confounds in translational research, which are frequently associated with adverse chronic invasive pharmacotherapies.

关键词： pre-clinical models depression anxiety oral dosing animal welfare

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免疫放射法测定地方甲状腺肿病区居民血清促甲状腺激素的意义

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中华内科杂志 1991年第11期30卷 703-705+731页

作者：欧阳安苏天水张玉凤王建波李辰晨 Jerome m 赫希曼河南医科大学附属第一医院内科内分泌组美国加利福尼亚大学、洛杉矶医学院内科及内分泌研究室

在供碘盐25年的地方甲状腺肿病区(简称地甲肿病区),我们测定了村民的甲状腺机能和TSH浓度,发现居民的血清FT和TSH(IRmA)浓度都高于、而FT均低于非地甲肿痛区的对照组;而放免法(ria)测定的TSH、T及T都在正常范围,两地区的结果对比无明显... 详细信息

在供碘盐25年的地方甲状腺肿病区(简称地甲肿病区),我们测定了村民的甲状腺机能和TSH浓度,发现居民的血清FT和TSH(IRmA)浓度都高于、而FT均低于非地甲肿痛区的对照组;而放免法(ria)测定的TSH、T及T都在正常范围,两地区的结果对比无明显差异,说明放免法不如免疫放射法(IRmA)敏感。用后者测定的TSH浓度提示该地甲肿病区的健康居民有亚临床性甲状腺功能低下。因此认为用免疫放射法测血清TSH浓度和定期测FT浓度是监测碘盐预防计划和居民对碘的生理反应的最好生化法。

关键词：促甲状腺激素免疫放射法甲状腺肿,地方性

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Clinical and Spatial Characteristics of Severe Acute Respiratory Syndrome by COVID-19 in Indigenous of Brazil

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Advances in Infectious Diseases 2021年第4期11卷 441-454页

作者： Daniele melo Sardinha Karla Valéria Batista Lima Ana Lúcia da Silva Ferreira Juliana Conceição Dias Garcez Thalyta m.riany Rêgo Lopes Ueno Yan Corrêa Rodrigues Anderson Lineu Siqueira dos Santos Rosane do Socorro Pompeu de Loiola Ricardo José de Paula Souza e Guimarães Luana Nepomuceno Gondim Costa Lima Programa de Pós-Graduaç ã o em Epidemiologia e Vigilancia em Saúde Instituto Evandro Chagas (PPGEVS/IEC) Ananindeua Brazil Programa de Pós-Graduaç ã o em Biologia Parasitária na Amazô nia Instituto Evandro Chagas and Universidade do Estado do Pará (PPGBPA/IEC/UEPA) Belém Brazil Programa de Pós-Graduaç ã o em Biologia Parasitária na Amazô nia Instituto Evandro Chagas and Universidade do Estado do Pará (PPGBPA/IEC/UEPA) Belém Brazil Secretaria de Estado de Saúde Pública do Pará(SESPA) Belém Brazil

The new coronavirus (SARS-CoV-2) broke out in Wuhan in China in December 2019, causing severe pneumonia and deaths, soon in march 2020, it reached pandemic level, affecting several countries including Brazil. The disease was named COVID-19, with characteristics of most infected having mild and moderate symptoms and a part severe symptom. The disease has already reached 158 ethnic groups, which have high vulnerability and limited access to health services. The objective is to investigate the clinical and spatial characteristics of Severe Acute Respiratory Syndrome of COVID-19 in the indigenous peoples of Brazil. It is an epidemiological, cross-sectional, analytical ecological study, based on data from the OpenDataSUS platform from 01/01/2020 to 31/08/2020. Profile variables, signs and symptoms and risk factors/comorbidities. The data were analyzed by Bioestat 5.3. There were 1,207 cases and 470 deaths. Profile: male gender (59.48%) means age 53 years. Signs and symptoms: fever (74.23%), cough (77.71%), sore throat (35.62%), dyspnea (69.34%), respiratory discomfort (62.80%), O2 saturation mortality: dyspnea (80.0%) and O2 saturation morbidities (45.89%) were associated with deaths (54.04%). About comorbidities, chronic cardiovascular diseases represented (18.97%) and Diabetes mellitus (18.97%), and associated with deaths: Chronic Cardiovascular Disease (24.46%). Being admitted to the ICU has a risk of death in (OR-3.96- mination of COVID-19 in this population, that COVID-19 evolves in the same intensity, however, the indigenous have vulnerabilities that can increase the impact of the pandemic in this population.

关键词： Severe Acute Respiratory Syndrome Indigenous People SARS-CoV-2 COVID-19 Brazil

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中欧地区COL7A1基因高发的第425位碱基A→G剪接位点突变和新发现的突变:对未来进行营养不良性大疱性表皮松解症突变检测的意义

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世界核心医学期刊文摘（皮肤病学分册） 2005年第8期1卷 16-17页

作者： Csikászik A. m. Csikós m. Szocs H.I. 李政霄 Lós Department of DermatologySemmelweisUniversityMá ria u. 41 H- 1085 Budapest Hungary Lós DepartmentofDermatologySemmelweisUniversityMá ria u. 41 H- 1085 Budapest Hungary

Background: mutations in the type VII collagen gene (COL7A1) are responsible for dominant and recessive forms of dystrophic epidermolysis bullosa (DEB). These mutations are usually specific for individual families; only a few cases of recurring mutations have been identified. Objectives: Forty- three unrelated Hungarian and German patients with different DEB phenotypes were screened for novel and recurrent COL7A1 mutations. methods: All patients were classified based on clinical and genetic findings,skin immunofluorescent antigen mapping, and electron microscopic studies. mutation analysis was performed by amplification of genomic DNA with polymerase chain reaction using COL7A1- specific primers, heteroduplex analysis, and direct nucleotide sequencing. Restriction endonuclease digestion was used for family screening and mutation verification. Results: In this group of patients, the splice- site mutation 425A → G was observed frequently, in 11 of 86 alleles (12- 8% ), once in homozygous form and in nine cases in heterozygous form. One of 100 control alleles from clinically unaffected individuals also carried the mutation. We also identified three novel mutations: the 976- 3C → A splice- site mutation, and the 4929 del T and 8441- 15del20 deletions. Conclusions: High recurrence of the splice- site mutation 425A → G in central European patients with DEB should be taken into account when designing COL7A1 mutation detection strategies. Reporting of three novel COL7A1 mutations in this study further emphasizes the molecular heterogeneity of DEB and provides more information for studies on genotype- phenotype correlations in different DEB subtypes.

关键词： COL7A1基因剪接位点突变营养不良性突变检测基因突变电镜检查聚合酶链反应荧光抗原扩增基因遗传学检查

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构音障碍的感知分析在皮质基底节变性和帕金森病鉴别诊断中的作用

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世界核心医学期刊文摘（神经病学分册） 2006年第5期2卷 38-39页

作者： Ozsancak C Auzou P Jan m 张慧 Service de Neurologie Centre Hospitalier de Boulogne Sur Mer 62200 Boulogne sur Mer FranceDr.

Objective: To characterize the dysarthria in patients with corticobasal degeneration (CBD) and determine if analysis of speech in isolation helps to distinguish CBD patients from patients with Parkinson’s disease (PD). methods: 60 subjects were assessed by means of perceptual analysis of speech: 15 patients with CBD, 15 patients with PD and 30 control subjects. A detailed profile was furnished with the help of 33 perceptual items. A global perceptual approach was used to classify patients by judges blind to the medical diagnosis. Rating scales were adapted to quantify the degree of spasticity and hypokinesia in the speech of each patient. Results: Dysarthria was frequent in CBD even though it remained mild for a long period of time. Group analysis revealed the importance of temporal errors of speech control in CBD patients while voice disturbances were most frequent in PD patients. However, attempts to classify patients according to global perceptual analysis remained below a reasonable level of clinical acceptability. Finally, even though the widespread neuropathological changes suggest that deviant speech dimensions of several types of dysarthria might be found in CBD, evidence for a mixed dysarthria with presence of spastic elements could not be established. Conclusion: The findings support the view that even though perceptual analysis is mandatory in the management of dysarthric patients, it does not help in the clinical differential diagnosis of CBD.

关键词：皮质基底节变性构音障碍鉴别诊断帕金森病感知患者特征 CBD 病理学变化语言分析评定量表

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用二种血清学试验对牛白血病病毒母源抗体持续时时的检测

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浙江畜牧兽医 1985年第1期 40-41页

作者： m.J.Burridge 裘永浩

本文应用牛白血病病毒糖蛋白抗原对母源抗体的持续时间进行了研究。研究是以已感染牛白血病病毒的母牛所生小牛进行的。但是这些小牛没有胎内感染的迹象。用琼脂凝胶扩散试验(以下简称AGIT)铡定定139头小牛,母源抗体检出的持续时间为不... 详细信息

本文应用牛白血病病毒糖蛋白抗原对母源抗体的持续时间进行了研究。研究是以已感染牛白血病病毒的母牛所生小牛进行的。但是这些小牛没有胎内感染的迹象。用琼脂凝胶扩散试验(以下简称AGIT)铡定定139头小牛,母源抗体检出的持续时间为不到1个月至6个月(平均2.9个月。通过对24头小牛的比较研究,有22头用放射免疫沉淀分析法(以下简称ria)比AGIT测到的母源抗体持续时间要长1—5个月。在这24头小牛中,母源抗体的平均持续时间用AGIT测到的为3.8个月(最短2个月。最长6个月);而用ria测到的平均为6.0个月(最短4个月,最长9个月)。

关键词：母源抗体持续时间平均 AG 检出个月连续时间牛白血病病毒月龄血清学试验

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