Snai-1 and Epithelial-Mesenchymal Transition-Related Protein Immunoexpression in Canine Mammary Carcinomas

作     者：Breno S.Salgado Rafael M.Rocha Fernando A.Soares Fátima Gartner Noeme S.Rocha 

作者机构：Departamento de PatologiaFaculdade de Medicina de BotucatuUniversidade Estadual Paulista(UNESP)BotucatuBrazil Laboratório de Patologia Investigativa e ComparadaDepartamento de Clínica VeterináriaFaculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista(UNESP)BotucatuBrazil Departamento de Anatomia PatológicaHospital A.C.CamargoFundacao Antonio PrudenteSao PauloBrazil Institute of Pathology and Molecular Immunology(IPATIMUP)OportoPortugal 

出 版 物：《Advances in Breast Cancer Research》 (乳腺癌（英文）)

年 卷 期：2014年第3卷第4期

页      面：111-117页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The National Council of Technological and Scientific Development,(CNPq),Brazil,provided financial support for Breno Salgado through the master research grant 130358/2010-0 for Noeme Rocha through the research grant 479178/2010-0 The Sao Paulo Research Foundation(FAPESP)provided financial support for Noeme Rocha through the research grants 2008/57309-5 and 2010/51596-2 

主　　题：EMT S100A4 Keratin Mammary Tumors Dog 

摘      要：Epithelial-mesenchymal transition (EMT) is defined as switching of polarized epithelial cells to a migratory fibroblastoid phenotype. EMT is known to be involved in the progression and metastasis of various cancers in humans, but this specific process is still little explored in the veterinary literature. The aim of this research was to evaluate the expression of EMT-related proteins in canine mammary carcinomas (CMCs). The expression of six EMT-related proteins in 94 CMCs of female dogs was evaluated by immunohistochemistry using a tissue array method. Additionally, clinicopathological characteristics were compared with the expression of EMT-related proteins. Loss of epithelial protein and/or acquisition of the expression of mesenchymal proteins were observed in CMCs. Loss of epithelial protein and/or acquisition of the expression of mesenchymal proteins were observed, particularly in tumors with evidence of stromal invasion;however, significance was only observed between the S100A4 and vascular invasion. In addition, Snai-1 nuclear immunoexpression was significantly related to E-cadherin loss. In conclusion, loss of epithelial proteins and/or the acquisition of mesenchymal proteins are associated with EMT and may have an important role in the evaluation of CMC patients. The unique immunoexpression pattern of Snai-1 could help to distinguish between an adenoma and a non-metastatic carcinoma and seems to be related to conversion of myoepithelial cells to a complete mesenchymal-like phenotype. Loss of E-cadherin and cytokeratin and change of immunoexpression pattern of Snai-1, N-cadherin, S100A4 and MMP-2 indicate the occurrence of EMT in canine mammary carcinomas and should result in an en bloc resection or a close follow-up.