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Endoscopic ultrasound fine needle aspiration vs fine needle biopsy in solid lesions:A multi-center analysis

World Journal of Clinical Cases 2021年第34期9卷 10507-10517页

作者： diogo Turiani Hourneaux Moura Thomas R McCarty Pichamol Jirapinyo Igor Braga Ribeiro Galileu Ferreira Ayala Farias Antonio Coutinho Madruga-Neto Marvin Ryou Christopher C Thompson Gastrointestinal Endoscopy Unit University of Sao Paulo School of MedicineSão PauloSP 05403-010Brazil Division of Gastroenterology Hepatology and EndoscopyBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115United States Division of Gastrointestinal Endoscopy University of São Paulo Medical SchoolSão PauloSP 01246-903Brazil

BACKGROUND While endoscopic ultrasound(EUS)-guided fine needle aspiration(FNA)is considered a preferred technique for tissue sampling for solid lesions,fine needle biopsy(FNB)has recently been *** To compare the accuracy of FNB vs FNA in determining the diagnosis of solid *** A retrospective,multi-center study of EUS-guided tissue sampling using FNA vs FNB *** outcomes included diagnostic test characteristics(i.e.,sensitivity,specificity,accuracy),use of rapid on-site evaluation(ROSE),and adverse *** analyses were performed by type of lesion and diagnostic yield with or without ROSE.A multivariable logistic regression was also *** A total of 1168 patients with solid lesions(n=468 FNA;n=700 FNB)underwent EUS-guided *** age was 65.02±12.13 ***,sensitivity,specificity and accuracy were superior for FNB vs FNA(84.70%vs 74.53%;99.29%vs 96.62%;and 87.62%vs 81.55%,respectively;P0.05).Multivariate analysis showed no significant predictor for better *** subgroup analyses,FNB was superior to FNA for non-pancreatic lesions;however,there was no difference between the techniques among pancreatic *** adverse event was reported in each *** FNB is superior to FNA with equivalent diagnostic test characteristics compared to FNA+ROSE in the diagnosis of non-pancreatic solid *** results suggest that EUS-FNB may eliminate the need of ROSE and should be employed as a first-line method in the diagnosis of solid lesions.

关键词： Endoscopic ultrasound-guided tissue acquisition Fine needle aspiration Fine needle biopsy Solid lesions Endoscopic ultrasound Cancer

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Second-growth and small forest clearings have little effect on the temporal activity patterns of Amazonian phyllostomid bats

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Current Zoology 2020年第2期66卷 145-153页

作者： Ricardo ROCHA Adria Lopez-Baucells Fabio Z.Farneda diogo F.Ferreira Ines SiLVA Marta AcAcio Jorge M.Palmeirim Christoph F.J.M eyer Centre for Ecology Evolution and Environmental ChangesFaculty of SciencesUniversity of LisbonLisbon1749-016Portugal Biological Dynamics of Forest Fragments Project National Institute for Amazonian Research and Smithsonian Tropical Research InstituteManaus69011-970Brazil Conservation Science Group University of CambridgeCambridgeCB23EJUK Granollers Museum of Natural Sciences Granollers08402Spain Department of Ecology Federal University of Rio de JaneiroRio de Janeiro21941-902Brazil CIBI0-lnBI0 Research Centre in Biodiversity and Genetic ResourcesUniversity of PortoVairao4485-661Portugal Conservation Ecology Program School of Bioresources and TechnologyKing Mongkufs University of Technology ThonburiBangkok10150Thailand School of Environmental Sciences University of East AngliaNorwichNR47TJUKand'Ecosystems Environment Research Centre(EERC) School of ScienceEngineering and EnvironmentUniversity of SalfordSalfordM54WTUK

Secondary forests and human-made forest gaps are conspicuous features of tropical ***,behavioral responses to these aspects of anthropogenically modified forests remain poorly ***,we analyze the effects of small human-made clearings and secondary forests on tropical bats by examining the guild-and species-level activity patterns of phyllostomids sampled in the Central Amazon,***,we contrast the temporal activity patterns and degree of temporal overlap of 6 frugivorous and 4 gleaning animalivorous species in old-growth forest and second-growth forest and of 4 frugivores in old-growth forest and forest *** activity patterns of frugivores and gleaning animalivores did not change between old-growth forest and second-growth,nor did the activity patterns of frugivores between old-growth forest and ***,at the species level,we detected significant differences for Artibeus obscurus(old-growth forest ***-growth)and ***(old-growth forest ***).The degree of temporal overlap was greater than random in all sampled ***,for frugivorous species,the degree of temporal overlap was similar between old-growth forest and second-growth;whereas for gleaning animalivores,it was lower in second-growth than in old-growth *** the contrary,forest clearings were characterized by increased temporal overlap between *** in activity patterns and temporal overlap may result from differential foraging opportunities and dissimilar predation ***,our analyses suggest that activity patterns of bats in second-growth and small forest clearings,2 of the most prominent habitats in humanized tropical landscapes,varies little from the activity patterns in old-growth forest.

关键词： Amazon behavior forest gaps niche partitioning secondary forest temporal overlap

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The neurosphere assay:an effective in vitro technique to study neural stem cells

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Neural Regeneration Research 2021年第11期16卷 2229-2231页

作者： Rita Soares Filipa F.Ribeiro diogo M.Lourenço Rui S.Rodrigues João B.Moreira Ana M.Sebastião Vanessa A.Morais Sara Xapelli Instituto de Medicina Molecular João Lobo Antunes Faculdade de MedicinaUniversidade de LisboaLisboaPortugal Instituto de Farmacologia e Neurociências Faculdade de MedicinaUniversidade de LisboaLisboaPortugal Instituto de Biologia Molecular Faculdade de MedicinaUniversidade de LisboaLisboaPortugal

Neural stem cells(NSCs)are known to be present in the adult mammalian brain where they constitutively differentiate into the neuronal,astroglial,and oligodendroglial lineages,in defined processes termed neurogenesis,ast rogl iogenesis and oligodendrogenesis,respectively(reviewed in Braun and Jessberger,2014).During brain development,NSCs are present throughout the brain,becoming progressively restricted to defined brain *** the adult brain,NSCs are mainly present in areas classically known as neurogenic niches,*** subventricular zone(SVZ),along the lateral walls of the lateral ventricles,and the subgranular zone,located in the dentate gyrus(DG)of the hippocampus.

关键词： walls neural becoming

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A Pt/MnV_(2)O_(6) nanocomposite for the borohydride oxidation reaction

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Journal of Energy Chemistry 2021年第4期30卷 428-436页

作者： Jadranka Milikic Marta Martins Ana S.Dobrota Gamze Bozkurt Gulin S.P.Soylu Ayse B.Yurtcan Natalia V.Skorodumova Igor A.Pasti Biljana Sljukic diogo M.F.Santos Faculty of Physical Chemistry University of BelgradeStudentski trg 12-1611158 BelgradeSerbia CeFEMA Instituto Superior TécnicoUniversidade de LisboaLisbonPortugal Project Coordination Implementation and Research Center Erzurum Technical UniversityErzurumTurkey Faculty of Engineering Chemical Engineering DepartmentIstanbul University-Cerrahpasa34320 AvcilarIstanbulTurkey Department of Chemical Engineering Atatürk UniversityErzurumTurkey Department of Materials Science and Engineering School of Industrial Engineering and ManagementKTH-Royal Institute of TechnologyBrinellvägen 2310044 StockholmSweden Department of Physics and Astronomy Uppsala UniversityBox 51675120 UppsalaSweden

Problems associated with carbon support corrosion under operating fuel cell conditions require the identification of alternative supports for platinum-based nanosized *** supported on manganese vanadate(Pt/MnV_(2)O_(6))was prepared by microwave irradiation method and characterized using X-ray diffraction,Fourier-transform infrared spectroscopy,X-ray photoelectron spectroscopy,scanning electron microscopy with energy dispersive spectroscopy,and transmission electron *** borohydride oxidation reaction(BOR)on Pt/MnV_(2)O_(6) was studied in highly alkaline media using voltammetry,chronoamperometry,and electrochemical impedance *** electrocatalytic activity of Pt/MnV_(2)O_(6) was also compared with that of commercial Pt/C(46 wt%Pt)*** apparent activation energy of BOR at Pt/MnV_(2)O_(6) was estimated to be 32 k J mol^(-1) and the order of reaction to be 0.51,indicating that borohydride hydrolysis proceeds in parallel with its ***-term stability of Pt/MnV_(2)O_(6) under BOR typical conditions was observed.A laboratory-scale direct borohydride fuel cell assembled with a Pt/MnV_(2)O_(6) anode reached a specific power of 274 W g^(-1).Experimental results on Pt/MnV_(2)O_(6) were complemented by DFT calculations,which indicated good adherence of Pt to MnV_(2)O_(6),beneficial for electrocatalyst stability.

关键词： Borohydride oxidation reaction Platinum-based electrocatalyst Manganese vanadate Density functional theory

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Nuclear RAG1 sequence data provide a test of competing evolutionary hypotheses of otophysan fishes (Teleostei:Otocephala)

Nuclear RAG1 sequence data provide a test of competing evolu...

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中国海洋湖沼动物学会鱼类学分会第七届会员代表大会暨朱元鼎教授诞辰110周年庆学术研讨会

作者：彭作刚何舜平 diogo Rui Institute of Hydrobiology Chinese Academy of Sciences Laboratory of Functional and Evolutionary Morphology University of LiegeB-4000 LiegeBelgium

The monophyletic group of teleostean fish known as the Otophysi(ostariophysan groups excluding Gonorynchiformes),represents about two third of all freshwater fishes and constitutes one of the most diverse vertebrate taxa,including the Cypriniformes(carps and loaches),Characiformes (piranhas and relatives),Siluriformes(catfishes),and Gymnotiformes(knifefishes).To date,there were five competing evolutionary hypotheses regarding the four otophysan groups(orders).The phylogenetic relationship within otophysan fishes remains highly *** present study, approximate 1170 bp nuclear RAG1 sequences of 13 otophysan fishes plus five RAG1 sequences of related groups were retrieved from *** phylogenetic trees,constructed using the maximum parsimony(MP),neighbor-joining(NJ),maximum likelihood(ML)and Bayesian methods,show an identical topology for the four otophysan *** competing otophysan topologies were then tested by using the approximately unbiased test,the Kishino-Hasegawa test,and the Shimodaira-Hasegawa *** results show that the grouping ((((Characiformes,Siluriformes), Gymnotiformes),Cypdniformes),outgroups)is the most likely but there is no significant difference between this one and the other two alternative hypotheses at 5% significant level— ((((Gymnotiformes,Siluriformes),Characiformes),Cypriniformes),outgroups),and ((((Characiformes,Gymnotiformes),Siluriformes),Cypriniformes),outgroups).

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Carcass image segmentation using CNN-based methods

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Information Processing in Agriculture 2021年第4期8卷 560-572页

作者： diogo Nunes Goncalves Vanessa Aparecida de Moares Weber Julia Gindri Bragato Pistori Rodrigo da Costa Gomes Anderson Vicoso de Araujo Marcelo Fontes Pereira Wesley Nunes Goncalves Hemerson Pistori Federal University of Mato Grosso do Sul Av.Costa e Silvas/nBairro UniversitarioCampo GrandeMS 79070-900Brazil INOVISAO Dom Bosco Catholic UniversityAvenida Tamandare6000CP 100Jardim SeminarioCampo GrandeMS 79117-900Brazil EMBRAPA Gado de Corte Av.Radio Maia830Vila PopularCampo GrandeMS 79106-550Brazil State University of Mato Grosso do Sul Av.Dom Antonio Barbosa4155Vila Santo AmaroCampo GrandeMS 79115-898Brazil

Carcass grading can be used as an important metric to determine meat ***,carcass grading is usually performed by a specialist,making it a subjective and errorprone *** increase the accuracy of such task,image-based systems have been proposed in the *** of the most important parts of an image-based system is the image segmentation,which aims to identify the regions of the carcass in the *** this paper,we propose the use of two recent image segmentation methods called Superpixel+CNN(Convolutional Neural Network)and *** evaluate both methods,we have also built a dataset of carcass images and their *** of approximately 96%of pixel accuracy show the robustness of the methods in carcass image *** novelty of this work is the proposal and comparison of recent deep learning methods that use CNN and superpixels in carcass *** this way,the methods can be used in carcass grading systems to increase the accuracy of the grading task.

关键词： Carcass grading Image segmentation Convolutional neural networks Superpixels

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An Overview of SWIPT Circuits and Systems

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ZTE Communications 2022年第2期20卷 13-18页

作者： Ricardo TORRES diogo MATOS Felisberto PEREIRA Ricardo CORREIA Nuno Borges CARVALHO Department of Electronics telecommunications and informationUniversity of AveiroAveiro 3810Portugal Institute of Telecommunications Aveiro 3800Portugal CISeD-Research Center in Digital Services Polytechnic of ViseuViseu 3500Portugal Sinuta SA. Estarreja 3860Portugal AlmaScience Colab Madan ParqueCaparica 2829-516Portugal

From a circuit implementation perspective,this paper presents a brief overview of simultaneous wireless information and power transmission(SWIPT).By using zero-power batteryless wireless sensors,SWIPT mixes wireless power transmission with wireless communications to allow the truly practical implementation of the Internet of Things as well as many other *** this paper,technical backgrounds,problem formation,state-of-the-art solutions,circuit implementation examples,and system integrations of SWIPT are presented.

关键词： simultaneous wireless information and power transmission(SWIPT)systems backscatter communication low power commu⁃nication

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Oncologic aspects of the decision-making process for surgical approach for colorectal liver metastases progressing during chemotherapy

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World Journal of Gastrointestinal Surgery 2022年第9期14卷 877-886页

作者： Raphael L C Araujo Camila G C Y Carvalho Carlos T Maeda Jean Michel Milani diogo G Bugano Pedro Henrique Z de Moraes Marcelo M Linhares Department of Surgery Universidade Federal de São PauloSão Paulo 04024-002Brazil Department of Oncology Hospital Israelita Albert EinsteinSão Paulo 05652-900Brazil Department of Surgical Oncology Hospital e Maternidade Brasil Rede D'Or São LuizSanto André09030-590São PauloBrazil

Colorectal cancer represents the third most diagnosed malignancy in the *** liver is the main site of metastatic disease,affected in 30%of patients with newly diagnosed *** resection is considered the only potentially curative treatment for colorectal liver metastasis(CRLM),with a 5-year survival rate ranging from 35%to 58%.However,up to 80%of patients have initially unresectable disease,due to extrahepatic disease or bilobar multiple liver *** availability of increasingly effective systemic chemotherapy has contributed to converting patients with initially unresectable liver metastases to resectable disease,improving long-term outcomes,and accessing tumor *** recent years,response to preoperative systemic chemotherapy before liver resection has been established as a major prognostic *** studies have demonstrated that patients with regression of hepatic metastases while on chemotherapy have improved outcomes when compared to patients with stabilization or progression of the *** if disease progression during chemotherapy represents an independent negative prognostic factor,some patients may still benefit from surgery,given the role of this modality as the main treatment with curative intent for patients with *** selected cases,based on size,the number of lesions,and tumor markers,surgery may be offered despite the less favorable prognosis and as an option for non-chemo responders.

关键词： Colorectal liver metastases Oncology Disease progression Surgery Liver resection Hepatectomy

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Indications and outcomes of endoscopic resection for nonpedunculated colorectal lesions:A narrative review

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World Journal of Gastrointestinal Endoscopy 2021年第8期13卷 275-295页

作者： Endrit Shahini diogo Libanio Giacomo Lo Secco Antonio Pisani Alberto Arezzo Department of Gastroenterology and Digestive Endoscopy Unit National Institute of Research“Saverio De Bellis”Castellana Grotte(Bari)70013Italy Department of Gastroenterology Portuguese Oncology InstitutePorto 4200-072Portugal Department of Surgical Sciences University of TorinoTurin 10126Italy

In the last years,endoscopic techniques gained a crucial role in the treatment of colorectal flat *** the same time,the importance of a reliable assessment of such lesions to predict the malignancy and the depth of invasion of the colonic wall *** current unsolved dilemma about the endoscopic excision techniques concerns the necessity of a reliable submucosal invasive cancer assessment system that can stratify the risk of the post-procedural need for ***,this narrative literature review aims to compare the available diagnostic strategies in predicting malignancy and to give a guide about the best techniques to *** performed a literature search using electronic databases(MEDLINE/PubMed,EMBASE,and Cochrane Library).We collected all articles about endoscopic mucosal resection(EMR)and endoscopic submucosal dissection(ESD)registering the ***,we analyzed all meta-analyses comparing EMR vs ESD outcomes for colorectal sessile or nonpolypoid lesions of any size,preoperatively estimated as *** meta-analysis studies,mainly Eastern,were included in the analysis comparing 124 studies and overall 22954 patients who underwent EMR and ESD *** these,eighty-two were retrospective,twenty-four perspective,nine casecontrol,and six cohorts,while three were randomized clinical trials.A total of 18118 EMR and 10379 ESD were completed for a whole of 28497 colorectal sessile or non-polypoid lesions>5-10 mm in *** conclusion,it is crucial to enhance the preoperative diagnostic workup,especially in deciding the most suitable endoscopic method for radical resection of flat colorectal lesions at risk of underlying ***,the ESD necessitates further improvement because of the excessively time-consuming as well as the intraprocedural technical hindrances and related *** found a higher rate of en bloc resections and R0 for ESD than EMR for non-pedunculated colorectal ***,d

关键词： Colorectal cancer Adenoma detection High-resolution colonoscopy Chromoendoscopy Pit pattern Dysplasia

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Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases

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Animal Nutrition 2022年第2期9卷 184-192页

作者： Monica M.Costa Luís B.Pio Pedro Bule Vania A.Cardoso Marlene Duarte Cristina M.Alfaia diogo F.Coelho Joana A.Bras Carlos M.G.A.Fontes Jose A.M.Prates CIISA-Centro de Investigaçao Interdisciplinar em Sanidade Animal Faculdade de Medicina Veterinari1Universidade de Lisbo1Alto da Ajud11300-477 Lisbo1Portugal NZYTech-Genes and Enzymes Estrada do Paço do LumiarCampus do LumiarEdifício E1649-038 Lisbo1Portugal

Green macroalgae,e.g.,Ulva lactuca,are valuable bioactive sources of nutrients;but algae recalcitrant cell walls,composed of a complex cross-linked matrix of polysaccharides,can compromise their utilization as feedstuffs for monogastric *** study aimed to evaluate the ability of pre-selected Carbohydrate-Active enZymes(CAZymes)and sulfatases to degrade *** cell walls and release nutritive compounds.A databank of 199 recombinant CAZymes and sulfatases was tested in vitro for their action towards *** cell wall *** enzymes were incubated with the macroalga,either alone or in combination,to release reducing sugars and decrease fluorescence intensity of Calcofluor White stained cell *** individual action of a polysaccharide lyase family 25(PL25),an ulvan lyase,was shown to be the most efficient in cell wall *** ulvan lyase treatment,in triplicate measures,promoted the release of 4.54 g/L(P0.05),proteins(P?0.861)or pigments(P>0.05)was *** results highlight the capacity of a single recombinant ulvan lyase(PL25 family)to incompletely disrupt *** cell *** enzyme could enhance the bioaccessibility of *** bioactive products with promising utilization in the feed industry.

关键词： Macroalga Ulva lactuca Carbohydrate-active enzyme Ulvan lyase Cell wall degradation

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