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In-situ monitoring of nitrile-bearing pesticide residues by background-free surface-enhanced Raman spectroscopy

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Chinese Chemical Letters 2022年第1期33卷 519-522页

作者： Guorui Wu Wenshuai Li Weijun Du Aiqin Yue Jinzhong Zhao dingbin liu College of Agronomy Shanxi Agricultural UniversityTaigu 030801China College of Arts and Sciences Shanxi Agricultural UniversityTaigu 030801China State Key Laboratory of Medicinal Chemical Biology Research Center for Analytical Sciencesand Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China

In-situ monitoring of pesticide residues during crop growth or/and in related products is of great significance in avoiding the abuse of pesticides but remains challenging thus *** this report,we proposed a background-free surface-enhanced Raman spectroscopy(bf-SERS)platform to non-destructively track the nitrile-bearing pesticide residues in soybean leaves with high sensitivity and *** outstanding feature of the assay stems from the dramatic Raman enhancement effect of the 50 nm-sized gold nanoparticles(AuNPs)towards the pesticides and simultaneously the background-free Raman signal of the nitrile group in the so-called Raman-silent region(1800-2800 cm^(-1)).This bf-SERS assay was applied to evaluate the penetration effects of nitrile-bearing pesticides and monitor their residues in soybean leaves after rinsing with various solutions,providing a reliable tool for guiding the safe use of nitrile-bearing pesticides in agriculture.

关键词： Nitrile-bearing pesticide residues Background-free SERS In-situ detection Chemical sensing Food safety

Peripheral origin exosomal micro RNAs aggravate glymphatic system dysfunction in diabetic cognitive impairment

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药学学报:英文版 2023年第7期13卷 2817-2825页

作者： Lin Zhang Dongna Li Pengrong Yi Jiangwei Shi Mengqing Guo Qingsheng Yin dingbin liu Pengwei Zhuang Yanjun Zhang State Key Laboratory of Component-based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Haihe Laboratory of Modern Chinese Medicine Tianjin 301617China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 300193China State Key Laboratory of Medicinal Chemical Biology Research Center for Analytical Sciencesand Tianjin Key Laboratory of Molecular Recognition and BiosensingCollege of ChemistryNankai UniversityTianjin 300071China

Cognitive dysfunction is one of the common central nervous systems(CNS)complications of diabetes mellitus,which seriously affects the quality of life of patients and results in a huge economic *** glymphatic system dysfunction mediated by aquaporin-4(AQP4)loss or redistribution in perivascular astrocyte endfeet plays a crucial role in diabetes-induced cognitive impairment(DCI).However,the mechanism of AQP4 loss or redistribution in the diabetic states remains *** evidence suggests that peripheral insulin resistance target tissues and CNS communication affect brain homeostasis and that exosomal miRNAs are key *** and lipid metabolism disorder is an important pathological feature of diabetes mellitus,and skeletal muscle,liver and adipose tissue are the key target insulin resistance *** this review,the changes in exosomal miRNAs induced by peripheral metabolism disorders in diabetes mellitus were systematically *** focused on exosomal miRNAs that could induce low AQP4 expression and redistribution in perivascular astrocyte endfeet,which could provide an interorgan communication pathway to illustrate the pathogenesis of ***,the mechanisms of exosome secretion from peripheral insulin resistance target tissue and absorption to the CNS were summarized,which will be beneficial for proposing novel and feasible strategies to optimize DCI prevention and/or treatment in diabetic patients.

关键词： Diabetic cognitive impairment AQP4 Peripheral-central communication Exosomal miRNAs Central nervous system Glymphatic system Diabetes mellitus Astrocyte

117例肺泡灌洗液阳性老年下呼吸道感染患者病原菌分布及耐药性分析

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检验医学与临床 2024年第12期21卷 1806-1810页

作者：夏珺李泉张吉曾建涛吴永琴刘钉宾重庆市长寿区人民医院检验科重庆401220

目的分析肺泡灌洗液(BALF)阳性的老年下呼吸道感染患者相关病原菌的分布和耐药性,为老年下呼吸道感染的预防及合理用药提供依据。方法对该院2020年1月1日至2023年6月30日送检的高度怀疑下呼吸道感染的664例住院老年患者BALF标本进行培养... 详细信息

目的分析肺泡灌洗液(BALF)阳性的老年下呼吸道感染患者相关病原菌的分布和耐药性,为老年下呼吸道感染的预防及合理用药提供依据。方法对该院2020年1月1日至2023年6月30日送检的高度怀疑下呼吸道感染的664例住院老年患者BALF标本进行培养,采用微量肉汤稀释法进行药敏试验。结果BALF标本中分离出病原菌147株,BALF标本阳性率为22.1%(147/664)。排除来自同一患者的重复分离菌株后,分离的病原菌为117株。117株病原菌中革兰阳性菌占7.7%,革兰阴性菌占72.6%,真菌占19.7%。其中革兰阴性菌以鲍曼不动杆菌、铜绿假单胞菌和肺炎克雷伯菌为主;革兰阳性菌以肺炎链球菌、金黄色葡萄球菌为主;真菌以白色假丝酵母菌为主。分离出117株病原菌的下呼吸道感染患者中,医院获得性肺炎46例(39.3%),社区获得性肺炎71例(60.7%)。药敏试验结果显示:鲍曼不动杆菌对呋喃妥因和头孢唑啉耐药率最高,为100.0%,对亚胺培南、美罗培南耐药率均为84.0%,对多黏菌素B、米诺环素、头孢噻肟耐药率均为0.0%;铜绿假单胞菌对氨苄西林/舒巴坦耐药率最高,为85.7%,对复方磺胺甲噁唑、氯霉素、米诺环素耐药率分别为64.3%、78.6%、45.5%;肺炎克雷伯菌除对氨苄西林天然耐药外,对其他测试药物的耐药率在0.0%~35.3%;白色假丝酵母菌对伏立康唑、两性霉素B均敏感。结论老年下呼吸道感染患者BALF标本检出病原菌以革兰阴性菌为主,其中鲍曼不动杆菌的耐药情况较严重,真菌感染也应该引起重视。

关键词：下呼吸道感染肺泡灌洗液病原菌耐药性老年患者

Direct cytoplasm delivery of gold nanoparticles for real-time apoptosis detection

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Nano Research 2020年第3期13卷 853-860页

作者： Qiang Li Fengchao Wang Jie Yang dingbin liu Research Center for Analytical Sciences Institute of Polymer ChemistryState Key Laboratory of Medicinal Chemical Biologyand Tianjin Key Laboratory of Molecular Recognition and BiosensingCollege of ChemistryNankai UniversityTianjin300071China

Gold nanoparticles(AuNPs)assembled with fluorescent peptides through Au-S bonds(pep-AuNPs)have been widely used in biomolecular ***,due to the endo/lysosomal trapping after the nanoprobes enter cells,the direct delivery of AuNP probes into the cytoplasm for real-time imaging remains a difficult barrier for many cytoplasm-targeting ***,we prepare AuNP@gel by wrapping a multi-functional nanogel structure on the surface of a single AuNP probe by in-situ polymerization in order to directly deliver AuNP probes into the cell *** with the pep-AuNP probes,which are trapped inside lysosomes for long periods,the AuNP@gel probes use the proton-sponge effect to effectively disrupt endo/lysosomal membranes and remain in the *** addition,the AuNP@gel probes rapidly escape from endo/lysosomes to avoid the complex environment that interferes with the stability of the AuNP probes and the lysosomal-storage trigger the upregulation of oxidative stress into the *** nanogel structure enables the AuNP probes to avoid some detrimental effects and to achieve high-fidelity fluorescence signals in the *** to traditional strategies for lysosomal escape,this one-step in-situ polymerization procedure avoids the complicated modification of additional ligands and is generally applicable to peptide-,DNA-,and polymerlinked AuNP probes.

关键词： gold nanoprobes nanogel endo/lysosomal escape real-time imaging apoptosis detection

Using selenium-conjugated polyethylene glycol to enhance the stability of gold nanoparticles in biologically relevant samples

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Science China Chemistry 2019年第2期62卷 280-286页

作者： Yang Xue Bo Dong Xuehui liu Fengchao Wang Jie Yang dingbin liu State Key Laboratory of Medicinal Chemical Biology Research Center for Analytical Sciences and Tianjin Key Laboratory of Molecular Recognition and Biosensing College of Chemistry Nankai University Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

Using stabilizing agents to maintain the physicochemical properties of colloids in complex environments is crucial for their realworld applications. In this article, we describe how selenium-(Se-) terminated polyethylene glycol(PEG) can serve as a highaffinity stabilizing agent for gold nanoparticles(AuNPs). Compared to Au NPs modified with standard thiolated PEG(S-PEG),Se-PEG-coated Au NPs are much more stable under extreme conditions such as high/low pH, high salt content, and high temperatures. We demonstrate that the Se anchor can prevent the dissociation of PEG ligands from Au NP surfaces in living cells,where a higher concentration of biothiols is usually present. These results indicate that Se-PEG is an excellent stabilizing agent that may facilitate further studies on metal NPs for various complex and physiological systems.

关键词： selenium polyethylene glycol gold nanoparticles biological stabifity

Utilization of unmodified gold nanoparticles in colorimetric detection

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Science China(Physics,Mechanics & Astronomy) 2011年第10期54卷 1757-1765页

作者： HE Sha liu dingbin WANG Zhuo CAI KaiYong JIANG XingYu College of Bioengineering Chongqing University Chongqing 400044 China Chinese Academy of Sciences Key Laboratory for Biological Effects of Nanomaterials and Nanosafety National Center for NanoScience and Technology Beijing 100190 China

This review begins with an overview of the appealing properties and various applications of gold nanoparticles, and briefly summarizes recent advances in using unmodified gold nanoparticles to detect different kinds of targets including nucleic acids, proteins, metal ions and small organic molecules. The key point to the unmodified gold nanoparticle-based visual detection assay is to control dispersion and aggregation of colloidal nanoparticles by targets of interest, which usually relies on affinities between gold nanoparticles and targets. The degree of dispersion or aggregation can be visualized through the change of the solution color or the precipitation of nanoparticles from the solution. Thus, the existence of the target molecules can be trans-lated into optical signals and monitored by the naked eye conveniently. Finally, some future prospects of this research field are given.

关键词：金纳米粒子视觉检测修改比色法有机小分子金属离子目标分子发展前景

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动脉粥样硬化光学成像探针研究进展

高等学校化学学报 2022年第12期43卷 163-177页

作者：叶卓吉墨轩刘定斌河南师范大学化学化工学院新乡453007 南开大学化学学院药物化学生物学国家重点实验室天津300071

心血管疾病(Cardiovascular disease,CVD)是全球疾病致死的主要原因之一.动脉粥样硬化(Atherosclerosis,AS)是引发各种CVD的首要危险因素,其发生发展通常经历持续的慢性炎症过程.因此,及时高效地检测AS,对于早期评估、诊断和治疗CVD具... 详细信息

心血管疾病(Cardiovascular disease,CVD)是全球疾病致死的主要原因之一.动脉粥样硬化(Atherosclerosis,AS)是引发各种CVD的首要危险因素,其发生发展通常经历持续的慢性炎症过程.因此,及时高效地检测AS,对于早期评估、诊断和治疗CVD具有重要临床意义.光学探针成像拥有极高的灵敏度和空间分辨率以及超快的信号采集处理速度,被广泛应用于生物医学检测与成像.本文综合评述了6种常见用于AS成像的光学探针,涉及小分子荧光探针、聚集诱导发光(Aggregate-induced emission,AIE)纳米探针、量子点探针、上转换纳米探针、光声探针和多模态探针等;并对各种探针的优缺点进行了分析比较.在此基础上,展望了光学探针在AS成像领域的发展前景,并提出了相应建议.

关键词：光学探针动脉粥样硬化生物成像

Recent progress in the application of microfluidic systems and gold nanoparticles in immunoassays

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Science China Chemistry 2011年第8期54卷 1227-1232页

作者： CHEN WenWen LI TangSong HE Sha liu dingbin WANG Zhuo ZHANG Wei JIANG XingYu CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology Beijing 100190 China Graduate Schoold of the Chinese Academy of Sctences Beijing 100049 China Key Laboratory of Microsystems and Microstructures Manufacturing Ministry of Education Harbin Institute of Technology Harbin 150080 China College of Bioengineering Chongqing University Chongqing 400044 China

Immunoassays are useful for many bioassays. Many new techniques and materials are introduced into the immunoassay to improve the efficiency. This paper reviews recent progress in the application of microfluidic systems and gold nanoparticles in immunoassay. The micro/nano technologies and materials can offer good sensitivity, fast detection, cost-effectiveness and easy signal readout. In particular, the miniaturization of microfluidics and colorimetric assays based on gold nanoparticles have dramatically improved the efficiency of immunoassays.

关键词：金纳米粒子微流体系统免疫分析应用微米/纳米技术生物测定免疫证明成本效益

快速、可视化新冠病毒核酸检测——第54届国际化学奥林匹克试题第1题解析

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大学化学 2022年第12期37卷 1-5页

作者：刘定斌南开大学化学学院天津300071

新冠肺炎疫情在全球范围内造成了重大影响,发展快速、简便的新冠病毒检测方法至关重要。第54届国际化学奥林匹克试题第1题以胶体金及其在核酸检测中的应用为主要内容,考查了分析化学相关的基础知识。

关键词：胶体金核酸检测紫外-可见吸收光谱 Lambert-Beer定律