Advanced technologies for single-cell in situ protein profiling

作     者：Wen Zhou Xiaoyue Ni Chen Xie Quli Fan Dingbin Liu Wen Zhou;Xiaoyue Ni;Chen Xie;Quli Fan;Dingbin Liu

作者机构：State Key Laboratory of Organic Electronics and Information Displaysand Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM)Institute of Advanced Materials(IAM)Nanjing University of Posts&TelecommunicationsNanjing 210023China State Key Laboratory of Medicinal Chemical BiologyResearch Center for Analytical SciencesTianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2022年第65卷第1期

页      面：48-67页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：supported by the National Key R&D Program of China(2019YFA0210103) the National Natural Science Foundation of China(61905122,22174070,21775075,21977053) the Natural Science Foundation of Jiangsu Province(BK20190735) the Research Start-up Fund of Nanjing University of Posts and Telecommunications(NY220149,NY219006) the Fundamental Research Funds for the Central Universities,Nankai University(2122018165) 

主　　题：proteomics single-cell CyTOF immunofluorescence SERS 

摘      要：Cells are inherently heterogeneous to achieve a diverse spectrum of biological *** understand the underlying protein machinery that achieves these fascinating functions,it is important to develop advanced analytical methods that can profile proteins in their native environment,as protein expression,aggregation,degradation,and regulation define both normal physiology as well as *** and transcriptome sequencing have seen major advances at the single-cell levels,but comprehensive proteomic profiling is still *** conventional proteomic methods,such as enzyme-linked immunosorbent assay(ELISA),western blot,and protein chips,can characterize biomarkers of ***,these ensemble techniques are unsuitable for single-cell *** evidence has shown the significance of in situ,sensitive,quantitative,and multiplexed profiling of biomarkers in single cells for diagnosis and treatment ***,we review the recent development of advanced imaging and spectroscopy techniques,including mass cytometry,immunofluorescence,and surfaceenhanced Raman spectrometry(SERS)for single-cell proteomic *** also provide our view on the challenges and the outlook.