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Induction of Protection against Paraquat-induced Oxidative Damage by Abscisic Acid in Maize Leaves is Mediated through Mitogen-activated Protein Kinase

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Journal of Integrative Plant Biology 2009年第10期51卷 961-972页

作者： Hai-Dong ding Xiao-Hua Zhang Shu-Cheng XU Li-Li Sun Ming-yi Jiang A-ying Zhang yin-Gen Jin college of life sciences Nanjing Agricultural University Nanjing 210095 China college of Biosciences and Biotechnology Yangzhou University Yangzhou 225009 China college of life sciences Fuyang Teachers College Fuyang 236032 China National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

Mitogen-activated protein kinase （MAPK） cascade has been shown to be important components in stress signal transduction pathway. In the present study, protection of maize seedlings （Zea mays L.） against paraquat-generated oxidative toxicity by abscisic acid （ABA）, its association with MAPK and ZmMPK5, a candidate for MAPK were investigated. Treatment of maize leaves with exogenous ABA led to significant decreases in the content of malondialdehyde, the percentage of ion leakage and the level of protein oxidation （in terms of carbonyl groups） under paraquat （PQ） stress. However, such decreases were blocked by the pretreatment with two MAPK kinase inhibitors PD98059 and U0126. The damage caused by PQ was further aggravated by inhibitors. Two inhibitors also suppressed the total activities of the antioxidant enzymes superoxide dismutase （SOD, EC 1.***.1.1）, catalase （CAT, EC 1.***.1.6）, ascorbate peroxidase （APX, EC ***）, and glutathione reductase （GR, EC 1.***.4.2）. Besides, treatment with PQ stimulated the activation of a 46 kDa MAPK, which was identified as ZmMPK5 by in-gel kinase assay with immunoprecipitation. These results reveal that ABA-induced protection against PQ-generated oxidative damage is mediated through MAPK cascade in maize leaves, in which ZmMPK5, a candidate for MAPK, is demonstrated to be involved.

关键词： abscisic acid mitogen-activated protein kinase oxidative damage paraquat Zea mays

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温度和不同生长调节物质对水稻种胚发芽的影响

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天津农学院学报 2001年第3期8卷 37-39页

作者：丁得亮郭志义刘惠芬米红霞天津农学院农学系天津300384

试验选用水稻品种中花 1 5种子 ,在不同温度处理进行发芽试验 ,并用 GA3 赤霉素、6-BA、NAA 萘乙酸处理水稻种子 ,研究温度和不同生长调节物质对中花 1 5发芽的影响 .正交试验结果表明 :经 1 0 mg/ L GA3浸泡处理 3 5 h后于 2 6～ ... 详细信息

试验选用水稻品种中花 1 5种子 ,在不同温度处理进行发芽试验 ,并用 GA3 赤霉素、6-BA、NAA 萘乙酸处理水稻种子 ,研究温度和不同生长调节物质对中花 1 5发芽的影响 .正交试验结果表明 :经 1 0 mg/ L GA3浸泡处理 3 5 h后于 2 6～ 2 8℃恒温处理 5 d,对其种子发芽效果显著 ,6-BA、NAA效果次之 .

关键词：水稻中花15 温度生长调节物质正交试验发芽

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How to disentangle microbially functional complexity: an insight from the network analysis of C, N, P and S cycling genes

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science Bulletin 2019年第16期64卷 1129-1131页

作者： Bang-Xiao Zheng yi Zhao Qing-Fang Bi Guo-Wei Zhou Hong-Jie Wang Xiu-Li Hao Kai ding Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Faculty of Biological and Environmental sciences Ecosystems and Environment Research ProgrammeUniversity of HelsinkiNiemenkatu 73FI-15140 LahtiFinland Section for Microbial Ecology and Biotechnology Department of Plant and Environmental SciencesUniversity of CopenhagenThorvaldsenvej 40DK-1871 Frederiksberg CDenmark Key Laboratory of Subtropical Soil science and Plant Nutrition of Zhejiang Province College of Environmental&Resource SciencesZhejiang UniversityHangzhou 310058China Department of Microbiology and Ecosystem science Research Network'Chemistry Meets Microbiology’University of ViennaVienna A-1090Austria State Key Lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Microbial Ecophysiology Group Faculty of Biology/ChemistryUniversity of BremenD-28359 BremenGermany Institute for Environmental Genomics University of OklahomaNormanOK 73019USA State Key Laboratory of agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River) Ministry of AgricultureCollege of Resources and EnvironmentHuazhong Agricultural UniversityWuhan 430070China

A complete ecosystem is also a complex network in which multifarious species interact with each other to achieve system-level functions, such as nutrient biogeochemistry (1)Microbial community is commonly considered as the primary driving force of ecosystem nutrient mobilization and metabolism, especially carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane coupling process (2)The rise of metagenomics and high-throughput array (e.g. PhyloChip, GeoChip, etc.

关键词： net P and S cycling genes an insight from the network analysis of C How to disentangle microbially functional complexity

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Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation

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science china(life sciences) 2022年第5期65卷 969-987页

作者： Fenghua Zhang Yongkang Hao Xianmei Li yi Li ding Ye Ru Zhang Xiaosi Wang Mudan He Houpeng Wang Zuoyan Zhu Yonghua Sun State Key Laboratory of Freshwater Ecology and Biotechnology Hongshan LaboratoryInstitute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhan 430072China college of Advanced agricultural sciences University of Chinese Academy of SciencesBeijing 100049China

The surrogate reproduction technique,such as inter-specific spermatogonial stem cells(SSCs)transplantation(SSCT),provides a powerful tool for production of gametes derived from endangered species or those with desirable ***,generation of genome-edited gametes from a different species or production of gametes from a phylogenetically distant species such as from a different subfamily,by SSCT,has not ***,using two small cyprinid fishes from different subfamilies,Chinese rare minnow(gobiocypris rarus,for brief:Gr)and zebrafish(danio rerio),we successfully obtained Gr-derived genome-edited sperm in zebrafish by an optimized SSCT *** transplanted Gr SSCs supported the host gonadal development and underwent normal spermatogenesis,resulting in a reconstructed fertile testis containing Gr spermatids and zebrafish testicular somatic ***,the surrogate spermatozoa resembled those of host zebrafish but not donor Gr in morphology and swimming *** pou5f3 and chd knockout Gr SSCs were transplanted,Gr-derived genome-edited sperm was successfully produced in *** is the first report demonstrating surrogate production of gametes from a different subfamily by SSCT,and surrogate production of genome-edited gametes from another species as *** method is feasible to be applied to future breeding of commercial fish and livestock.

关键词： surrogate reproduction genome editing spermatogonial stem cell transplantation zebrafish Gobiocypris rarus

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The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of UbL40 expression^∞

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Journal of Integrative Plant Biology 2020年第7期62卷 1034-1056页

作者： Jing Jin Songtao Gui Qian Li ying Wang Hongyuan Zhang Zhixuan Zhu Hao Chen Yueyang Sun Yu Zou Xingguo Huang yi ding State Key Laboratory of Hybrid Rice Department of GeneticsCollege of Life SciencesWuhan UniversityWuhan 430072China Institute of Vegetable Wuhan Academy of Agricultural SciencesWuhan 430072China Wuhan Wuda Tianyuau Bio-Tech Co. Ltd.Wuhan 430070China

The thermosensitive genic male sterile 5(tms5)mutation causes thermosensitive genic male sterility in rice(Oryza sativa)through loss of RNase ZS1 function,which influences ubiquitin fusion ribosomal protein L40(UbL40)messenger RNA levels during male ***,we used ATAC-seq,combined with analysis of H3K9ac and H3K4me2,to identify changes in accessible chromatin during fertility conversion of the two-line hybrid rice Wux-iang S(WXS)derived from a mutant tms5 ***-thermore,RNA-seq and bioinformatic analyses identified specific transcription factors(TFs)in differentially accessible chromatin *** these TFs,only GATA10 targeted ***10 knockout mutations,which resulted in low expression of UbL40 and a tendency toward male fertility,confirmed that GATA10 regulated fertility conversion via themodulation of ***,GATA10 acted as a medi-ator for interactions with ERF65,which revealed that tran-scriptional regulation is a complex process involving multiple complexes of TFs,namely TF *** appears that the ERF141/MADS7/MADS50/MYB modules affect metabolic processes that control anther and pollen development,es-pecially cell wall *** analysis revealed that these modules directly or indirectly affect metabolic pathway-re-lated genes to coordinate plant growth with proper anther development,and furthermore,that GATA10 regulates fer-tility conversion via the modulation of UbL40 expression.

关键词： sativa fertility sterile

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Plasma metabolomics identifies metabolic alterations associated with the growth and development of cat

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动物模型与实验医学(英文) 2023年第4期6卷 306-316页

作者： Shuaiyang Liu Yun Chen Xiaoming Wang Shuang Wang Lan Bai Xu Cheng Juan Wan Yufeng Hu yi ding Xin Zhang Mingxing ding Hongliang Li Manli Hu School of Basic Medical science Wuhan UniversityWuhanChina Institute of Model Animal Wuhan UniversityWuhanChina Clinical Trial Centers Huanggang Central HospitalHuanggangChina college of Veterinary Medicine Huazhong Agricultural UniversityWuhanChina Key Laboratory of Cardiovascular Disease Prevention and Control Ministry of EducationSchool of Basic Medical ScienceGannan Medical UniversityGanzhouChina Gannan Innovation and Translational Medicine Research Institute Gannan Medical UniversityGanzhouChina

Background:The purpose of our study was to study the composition and content of the feline plasma metabolome revealing the critical metabolites and metabolic pathways associated with age during growth and ***:Blood samples were collected from juvenile and adult groups for blood routine tests and serum biochemistry ***-targeted metabolomics analyses of plasma were also performed to investigate changes in metabolites and metabolic ***:In this study,we found that the red blood cell counts,liver function indexes(albumin and gamma-glutamyl transpeptidase),and the concentration of triglyceride and glucose changed significant with growth and *** metabolomics results revealed that 1427 metabolites were identified in the plasma of young and adult *** of these metabolites belong to major classes of lipids and lipid-like *** most obvious age-related metabolites include reduced levels of chenodeoxycholate,taurocholate,cholate,and taurochenodeoxycholate but increased levels of L-cysteine and taurocyamine in the adult cat's *** metabolites are mainly involved in the primary bile acid biosynthesis pathway,the bile secretion pathway,and the taurine and hypotaurine metabolism ***:This study revealed many age-related metabolite alterations in the feline *** age-varying metabolites,especially in the bile acid biosynthesis and secretion metabolism pathways,indicate that the regulation of these pathways is involved in the growth and development of *** study promotes our understanding of the mechanism of feline growth and provides new insights into nutrition and medicine for cats of different ages.

关键词： bile acids cat growth metabolomics plasma

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Wing patterning genes of Nilaparvata lugens identification by transcriptome analysis, and their differential expression profile in wing pads between brachypterous and macropterous morphs

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Journal of Integrative Agriculture 2015年第9期14卷 1796-1807页

作者： LI Kai-yin HU ding-bang LIU Fang-zhou LONG Man LIU Si-yi ZHAO Jing HE Yue-ping HUA Hong-xia Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural University

The brown planthopper, Nilaparvata lugens is an economically important pest on rice plants. This species produces macropterous and brachypterous morphs in response to environmental cues, which makes it very dififcult to control. The molecular basis of wing patterning in N. lugens is stil unknown. It is necessary to identify wing patterning genes of N. lugens, and also to clarify the expression differences of wing patterning genes between macropterous and brachypter-ous morphs. High-throughput deep sequencing of transcriptome of N. lugens wing pad yielded 116 744 580 raw reads and 113 042 700 clean reads. Al the reads were assembled into 55 963 unigenes with an average length of 804 bp. With the E-value cut-off of 1.0E–5,18 359 and 2 883 unigens had hits in NCBI-NR (NCBI non-redundant protein sequences) and NCBI-NT (NCBI nucleotide sequences) databases, respectively. A total of 16 502 unigenes were assigned to GO (gene ontology) classiifcation, 9 709 ungenes were grouped into 26 COG (cluster of orthologous groups of proteins) classiifcations, and 6 724 unigenes were assigned to different KEGG (Kyoto encyclopedia of genes and genomes) path-ways. In total, 56 unigenes which are homologous to wing patterning genes of Drosophila melanogaster or Tribolium castaneum were identiifed. Out of the 56 unigenes, 24 unigenes were selected, and their expression levels across the ifve nymphal stages between macropterous strain and brachypterous strain were examined by qRT-PCR. Two-way ANOVA analysis showed that development stage had signiifcant effects on the expression level of al the 24 genes (P<0.05). The expression levels of 8 genes (Nlen, Nlhh, Nlsal, NlAbd-A, Nlwg, Nlvg, Nlexd and NlUbx) were signiifcantly affected by wing morph. This is the ifrst transcriptome analysis of wing pads of hemimetabolous insect, N. lugens. The identiifed wing patterning genes would be useful resource for future exploration of molecular basis of wing development. The 8 differential y expressed win

关键词： Nilaparvata lugens wing pad transcriptome wing patterning genes

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播期及生长调节剂对间作大豆倒伏和产量的影响

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西南大学学报（自然科学版） 2024年第9期46卷 19-34页

作者：傅健易靖邓磊刘卓柳宁张瑞丰陈松柏易泽林西南大学农学与生物科技学院重庆400715 重庆市农业技术推广总站重庆401121

以大豆、玉米带状复合种植模式为基础,大豆选用耐荫抗倒伏品种‘渝豆11’,玉米选用半紧凑品种‘成单30’,试验采用两因素裂区设计,主区为播期(A),A1为大豆、玉米同期播种,A2为大豆较玉米晚播14 d,副区为9种不同种类及浓度的植物生长调节... 详细信息

以大豆、玉米带状复合种植模式为基础,大豆选用耐荫抗倒伏品种‘渝豆11’,玉米选用半紧凑品种‘成单30’,试验采用两因素裂区设计,主区为播期(A),A1为大豆、玉米同期播种,A2为大豆较玉米晚播14 d,副区为9种不同种类及浓度的植物生长调节剂(B),分别于2022年春季和2023年春季在西南大学歇马科研基地进行试验.研究在大豆、玉米带状间作模式下不同播期及生长调节剂对间作大豆产量及其构成因素、根系形态特征和倒伏及茎秆形态特征的影响,研究结果如下:(1)播期及植物生长调节剂对间作大豆产量及其构成因素具有显著影响.播期上同期播种大豆产量高于晚播大豆(A1>A2),其中A1B5、A1B8处理优于其他处理,其两年平均产量较对照分别增长48.56%、40.92%.A1B5处理的单株粒数、单株粒质量、百粒质量最大,较对照两年平均分别提高33.96%、48.56%、10.93%.(2)播期及植物生长调节剂对间作大豆倒伏及相关指标具有显著影响.晚播大豆平均倒伏率较同期播种大豆增加了20.84%,抗折力、茎粗显著减少,株高、主茎节数显著增加,而节间长在两播期间无显著差异.(3)播期及植物生长调节剂影响间作大豆根系形态特征.在不同生育期,大豆总根长、根尖数总体上同期播种大豆优于晚播.在不同时期,整体上大豆总根长为S3307>DTA-6>6-BA,而大豆根尖数为DTA-6>S3307>6-BA.综上所述,在大豆、玉米带状间作模式下,在初花期喷施DTA-6的产量优于S3307和6-BA,同期播种喷施60 mg/L DTA-6产量最高,其次是50 mg/L S3307,但是S3307处理下的大豆倒伏率较DTA-6更低,且产量与DTA-6差异不显著,更适宜机械化收割,利于应用推广.

关键词：大豆、玉米间作播期生长调节剂倒伏产量

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Diversity and functional analysis of Chinese bumblebee gut microbiota reveal the metabolic niche and antibiotic resistance variation of Gilliamella

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Insect science 2021年第2期28卷 302-314页

作者： Zi-Jing Zhang Ming-Fei Huang Li-Fei Qiu Rui-Hao Song Zi-Xuan Zhang yi-Wen ding Xin Zhou Xue Zhang Hao Zheng college of Food science and Nutritional Engineering China Agricultural UniversityBeijingChina college of Plant Protection China Agricultural UniversityBeijingChina

Bumblebees play an important role in maintaining the balance of natural and agricultural ecosystems,and the characteristic gut microbiota of bumblebees exhibit significant mutualistic *** has the highest diversity of bumblebees;however,gut microbiota of Chinese bumblebees have mostly been investigated through cultureindependent ***,we analyzed the gut communities of bumblebees from Sichuan,Yunnan,and Shaanxi provinces in china through 16S ribosomal RNA amplicon sequencing and bacterial *** revealed that the bumblebees examined in this study harbored two gut enterotypes as previously reported:one is dominated by Gilliamella and Snodgrassella,and the other is distinguished by prevalent environmental *** gut compositions obviously varied among different individual *** then isolated 325 bacterial strains and the comparative genomic analysis of Gillianiella strains revealed that galactose and pectin digestion pathways were conserved in strains from bumblebees,while genes for the utilization of arabinose,mannose,xylose,and rhamnose were mostly *** two strains from the Chinese bumblebees possess the multidrug-resistant gene emrB,which is phylogenetically closely related to that from the symbionts of soil entomopathogenic *** contrast,tetracycline-resistant genes were uniquely present in three strains from the *** results illustrate the prevalence of strain-level variations in the metabolic potentials and the distributions of antibiotic-resistant genes in Chinese bumblebee gut bacteria.

关键词： antibiotic resistance bumblebee Gilliamella.,gut microbiota sugar metabolism

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Evolution and roles of cytokinin genes in angiosperms 2:Do ancient CKXs play housekeeping roles while non-ancient CKXs play regulatory roles?

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Horticulture Research 2020年第1期7卷 2486-2500页

作者： Xiaojing Wang Jing ding Shanshan Lin Decai Liu Tingting Gu HanWu Robert N.Trigiano Richard McAvoy Jinling Huang yi Li State Key Laboratory of Crop Genetics and Germplasm Enhancement and college of Horticulture Nanjing Agricultural UniversityNanjingP.R.China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN 37996-4560USA Department of Plant science and Landscape Architecture University of ConnecticutStorrsCT 06269USA State Key Laboratory of Crop Stress Adaptation and Improvement Key Laboratory of Plant Stress BiologySchool of Life SciencesHenan UniversityKaifengChina Department of Biology East Carolina UniversityGreenvilleNC 27858USA

Cytokinin oxidase/dehydrogenase(CKX)is a key enzyme responsible for the degradation of endogenous ***,the origins and roles of CKX genes in angiosperm evolution remain *** on comprehensive bioinformatic and transgenic plant analyses,we demonstrate that the CKXs of land plants most likely originated from an ancient chlamydial endosymbiont during primary *** refer to the CKXs retaining evolutionarily ancient characteristics as“ancient CKXs”and those that have expanded and functionally diverged in angiosperms as“non-ancient CKXs”.We show that the expression of some non-ancient CKXs is rapidly inducible within 15 min upon the dehydration of Arabidopsis,while the ancient CKX(AtCKX7)is not drought *** plants overexpressing a non-ancient CKX display improved oxidative and drought tolerance and root *** mutant studies have shown that non-ancient CKXs regulate organ development,particularly that of ***,ancient CKXs preferentially degrade cis-zeatin(cZ)-type cytokinins,while non-ancient CKXs preferentially target N6-(Δ2-isopentenyl)adenines(iPs)and trans-zeatins(tZs).Based on the results of this work,an accompanying study(Wang et ***://***/10.1038/s41438-019-0211-x)and previous studies,we hypothesize that non-ancient CKXs and their preferred substrates of iP/tZ-type cytokinins regulate angiosperm organ development and environmental stress responses,while ancient CKXs and their preferred substrates of cZs play a housekeeping role,which echoes the conclusions and hypothesis described in the accompanying report(Wang,*** *** and roles of cytokinin genes in angiosperms 1:Doancient IPTs play housekeeping while non-ancient IPTs play regulatory roles?Hortic Res 7,(2020).https://***/10.1038/s41438-019-0211-x).

关键词： Evolution drought ancient

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