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利用普通光学显微镜实现对微小物体三维体表参数的测量

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光学技术 2010年第3期36卷 328-332页

作者：陈丽雷亮马北河 ding dong 广东工业大学物理与光电工程学院广州510006 威廉港应用科技大学光学实验室德国威廉港26388

基于有较大的焦深的普通光学显微镜及3D成像软件,利用自制的可绕固定轴的升降平台及光纤辐照形态,根据视差原理构建了一个测量小型样品的三维表面参数的测试系统。以外形较复杂的医用牙科钻头为样品,研究了显微镜的放大倍数、用于构建... 详细信息

基于有较大的焦深的普通光学显微镜及3D成像软件,利用自制的可绕固定轴的升降平台及光纤辐照形态,根据视差原理构建了一个测量小型样品的三维表面参数的测试系统。以外形较复杂的医用牙科钻头为样品,研究了显微镜的放大倍数、用于构建三维图像的两幅相似图像的夹角与样品表面参数测量值之间的关系。通过与样品的标称值对比,实验中所用的毫米量级的样品,在合适的物镜组放大倍数及合适的夹角下,相对误差在5%范围内,表明所建立的测量系统可用于尺寸较小的样品表面参数的测量。

关键词：光学测量显微镜焦深表面参数 3D软件夹角

Sedimentary Characteristics of the Second Marine Layer During the Late Marine Isotope Stage 3 in Southern Yellow Sea and Their Response to the East Asian Monsoon

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Journal of Ocean University of China 2018年第5期17卷 1103-1113页

作者： ZHANG Yang LI Guangxue GUO Hongzhou LIU Yong WANG Haoyin XU Jishang ding dong QIAO Lulu MA Yanyan WANG Liyan LI Qian College of Marine Geosciences Ocean University of China Key Laboratory of Submarine Geoscience and Prospecting Techniques Ministry of Education Development Research Center China Geological Survey

We use the particle size of sediments in core YS01A to study the sedimentary environment of the mud deposit in the central South Yellow Sea of China during late Marine Isotope Stages 3 （MIS3; 40.5 kyr-31.3 kyr）. In addition, the East Asian Monsoon and its relationship with the North Atlantic Ocean climate change are discussed based on the sensitive grain-size calculation and the spectrum analysis. The results show that during late MIS3, the muddy area in the central South Yellow Sea experienced the evolution of coastal facies, shallow marine facies, coastal facies, and continental facies, with weak hydrodynamic conditions. Compared with other climate indicators, we found that there were many century to millennium-scale climate signals documented in the muddy area sediments in the central South Yellow Sea. According to our particle size results, three strong winter monsoon events occurred at 37.6kyr, 35.6kyr and 32.2kyr. The East Asian Winter Monsoon records in core YS01A are consistent with the Greenland ice core and the Hulu cave stalagmite 8180. The millennial and centennial scale cycles, which are 55 yr, 72 yr, 115 yr, 262 yr respectively, correspond to solar activity cycles, while the 1049 yr and 2941 yr cycles correspond to the Dansgaard-Oeschger cycles. These cycles indicate that the paleoclimate evolution of the area was controlled by the solar activities, with the high-latitude driving thermohaline circulation as the main energy conveyor belt, followed by the sea-air-land amplification of the winter monsoon variation in the central Yellow Sea in the late MIS3.

关键词： sedimentary environment central South Yellow Sea East Asian Monsoon sensitive grade calculation thermohaline circulation

An Effective On-line Surface Particles Inspection Instrument for Large Aperture Optical Element

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International Journal of Automation and computing 2017年第4期14卷 420-431页

作者： Wen-dong ding Zheng-Tao Zhang Da-Peng Zhang De Xu Hai-Bing Lv Xin-Xiang Miao Guo-Rui Zhou Hao Liu Research Center of Precision Sensing and Control Institute of Automation Chinese Academy of Sciences School of Computer and Control Engineering University of Chinese Academy of Sciences Laser Fusion Research Center China Academy of Engineering Physics

Surface particles growing in large aperture optical element （LAOE） have significant impact on LAOE＇s stable operation. It is a challenge for the online system to inspect the particles with long working distance, enough precision and high efficiency because of the system constraints. In this paper, an effective and portable inspection instrument is designed based on dark-field imaging principle. A Nikon lens and an industrial high definition （HD） camera are selected to construct the vision system to inspect particles of microns size spreading over hundreds of millimeters. Using two motors and other mechanical structure, the system can realize auto-focus and image rectification functions. The line light sources are installed on both sides of the LAOE in a sealed box while the vision system is portable and working outside the box. An adaptive binarization method is proposed to process the captured dark-field image. The distribution of particles on the LAOE＇s surface is investigated. Because of the high resolution of the captured image, the SSE2 instructions optimization method is used to reduce the time cost of the algorithm. Experiments show that the instrument can inspect LAOE effectively and accurately.

关键词： Dark-field imaging image rectification adaptive binarization particle inspection large aperture optical elements(LAOE).

Accelerated Cutaneous Wound Healing Using an Injectable Teicoplanin-loaded PLGA-PEG-PLGA Thermogel Dressing

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Chinese Journal of Polymer Science 2019年第6期37卷 548-559页

作者： Wei-Ke Xu Jing-Yu Tang Zhang Yuan Cai-Yun Cai Xiao-Bin Chen Shu-Quan Cui Peng Liu Lin Yu Kai-Yong Cai Jian-dong ding State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular Science Fudan University Key Laboratory of Biorheological Science and Technology Ministry of Education College of Bioengineering Chongqing University

Bacterial infection is a very troublesome issue in wound treatment, which stimulates exudate formation and severely delays the healing process. Herein, a thermogelling dressing system composed of two triblock copolymers of poly(D,L-lactic acid-co-glycolic acid)-b-poly(ethylene glycol)-b-poly(D,L-lactic acid-co-glycolic acid)(PLGA-PEG-PLGA) with different block lengths was developed to deliver teicoplanin(TPN), a glycopeptide antibiotic, for cutaneous wound repair. The TPN-loaded thermogel was a free-flowing sol at room temperature and formed a semi-solid gel at physiological temperature. In vitro studies demonstrated that the TPN-loaded thermogel system exhibited desired tissue adhesiveness and realized the sustained release of TPN in a fast-followed-slow manner for over three weeks. Furthermore, a full-thickness excision wound model in Sprague-Dawley(SD) rats was constructed to assess the efficacy of TPNloaded thermogel formulation. Gross and histopathologic observations implied that treatment with the thermogel formulation reduced inflammation response, promoted disposition of collagen, enhanced angiogenesis, and accelerated wound closure and maturity of SD *** combination of the bioactivity of TPN and the acidic nature of the thermogel matrix was responsible for such an enhanced wound healing process. Consequently, the TPN-loaded PLGA-PEG-PLGA thermogel is a good candidate of wound dressing for full-thickness excision wound healing.

关键词： Wound dressing PLGA-PEG-PLGA thermogel Anti-infection Teicoplanin Sustained release

The role of primary cilia in mechanical transmission of osteocyte based on a 3D finite element model

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Medicine in Novel Technology and Devices 2024年第1期21卷 31-38页

作者： Zhuang Han dong ding Yu-bo Fan Xin-tong Wu Xiao Yang Lian-wen Sun Key Laboratory of Biomechanics and Mechanobiology(Beihang University)Ministry of Education Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Department of Cardiology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

The primary cilium,as a mechanical receptor of osteocytes,participates in the regulation of osteocyte ***,how the length of osteocyte primary cilia changes with fluid shear stress(FSS)are unclear,and how the mechanical transmission within osteocytes altered by primary cilia is not well understood ***,the ciliary length changes of osteocyte under 15dyn/cm2 of FSS were experimentally detected,and then 3D finite element models of osteocyte primary cilia containing the basal body and axoneme were *** results showed that(1)The ciliary length of the CON group,FSS 1h,and FSS 6h were 3.71±1.34μm,3.79±1.04μm,and 1.24±0.73μm respectively,indicating the different durations of FSS might lead to the adaptive changes of cilium *** calculations showed(2)when the ciliary length became shorter with the ciliary angle stayed the same,the deformation and stress of the cell membrane and membrane skeleton was ***,the deformation and stress of the cilia membrane,basal body,the rotation angles of basal body were decreased,and those of cytoplasm,cytoskeleton,actin cortex and nucleus were also decreased;(3)With the decrease of the ciliary angle,the deformation and stress of the cilia membrane,basal body,as well as the rotation angles of basal body were *** of the cytoplasm,cytoskeleton,actin cortex,and nucleus were also increased except the cell membrane and membrane *** calculation results suggested the length and angle of the primary cilia,the deformation and stress of intracellular structures in osteocyte were altered with ciliary basal body,indicated the connection between the basal body and cytoskeleton may be a key factor that affected the mechanical transport in osteocytes across the cell *** finally promoted the adaptive change of ciliary length under FSS.

关键词： Primary cilia Osteocyte model Finite element analysis Mechanotransduction

Responses of the Sea Surface Temperature and Suspended Matter Concentration to ENSO Events in the Bohai Sea

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Journal of Ocean University of China 2023年第3期22卷 697-708页

作者： LIU Xue ding dong LI Guangxue ZHANG Yang LIU Shidong WANG Nan ZHONG Yi YANG Xue PAN Yufeng YU Di ZHANG Lei XUE Wenjing Key Laboratory of Submarine Sciences and Prospecting Techniques MOEand College of Marine GeosciencesOcean University of ChinaQingdao 266100China National Satellite Meteorological Center(National Center for Space Weather) China Meteorological AdministrationBeijing 100081China College of Oceanic and Atmospheric Sciences Ocean University of ChinaQingdao 266100China

The sea surface temperature(SST)and sea surface suspended matter concentration data in the Bohai Sea were collected by multiple NOAA satellites from 1985 to *** background calibration method was used to correct the systematic errors caused by the long-term observations through multiple NOAA satellites in this ***,we studied the long-term variations of the sea surface temperature(SST)and sea surface suspended matter concentration(SSC)and their response to ENSO events in the Bohai *** results show that the SST and SSC of the Bohai Sea have been increasing since 1985,with an average rate of 0.04℃per year for SST,which is higher than the global *** average increasing rate of SSC was 0.51 mg L^(-1)yr^(-1),which may be mainly due to the enhancement of winter monsoon in the *** winter,the SST and SSC of the Bohai Sea were higher in El Niño years than in La Niña *** El Niño years,the ocean circulations were the main factor affecting the variation in *** robust circulation system caused by the strong winter monsoon enhances the water exchange between the cold water of the Bohai Sea and the warm water of the Yellow Sea,resulting in a higher SST in La Niña *** summer,the SSTs of the Bohai Sea were lower in El Niño years than those in the La Niña years because of the decrease of the SST in the Western Pacific Ocean,the weakening of the subtropical high in the western Pacific,and its eastward shift caused by the El Niño events.

关键词： AVHRR Bohai Sea SST SSC ENSO

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Miniature magnetically anchored and controlled camera system for trocar-less laparoscopy

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World Journal of Gastroenterology 2017年第12期23卷 2168-2174页

作者： ding-hui dong hao-yang zhu yu luo hong-ke zhang jun-xi xiang fei xue rong-qian wu yi lv Department of Hepatobiliary Surgery First Affiliated Hospital Xi’an Jiaotong University

AIM To design a miniature magnetically anchored and controlled camera system to reduce the number of trocars which are required for *** The system consists of a miniature magnetically anchored camera with a 30° downward angle, an external magnetically anchored unit, and a vision output device. The camera weighs 12 g, measures Φ10.5 mm × 55 mm and has two magnets, a vision model, a light source, and a metal hexagonal nut. To test the prototype, the camera was inserted through a 12-mm conventional trocar in an ex vivo real liver laparoscopic training system. A trocar-less laparoscopic cholecystectomy was performed 6 times using a 12-mm and a 5-mm conventional trocar. In addition, the same procedure was performed in four canine *** Both procedures were successfully performed using only two conventional laparoscopic trocars. The cholecystectomy was completed without any major complication in 42 min(38-45 min) in vitro and in 50 min(45-53 min) using an animal model. This camera was anchored and controlled by an external unit magnetically anchored on the abdominal wall. The camera could generate excellent image. with no instrument *** The camera system we designed provides excellent optics and can be easily maneuvered. The number of conventional trocars is reduced without adding technical difficulties.

关键词： Trocar-less laparoscopy Magnetically anchored and controlled camera Minimally invasive surgery

Single-cell RNA sequencing of meiocytes and microspores reveals the involvement of the Rf4 gene in redox homeostasis of CMS-C maize

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The Crop Journal 2021年第6期9卷 1237-1247页

作者： Huaisheng Zhang Huili Yang Desheng Hu Bing Li Yanan Lin Wen Yao Zhanyong Guo Haochuan Li dong ding Zhanhui Zhang Yanmin Hu Yadong Xue Jihua Tang National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhou 450002HenanChina College of Life Sciences Henan Agricultural UniversityZhengzhou 450002HenanChina

Normal microsporogenesis is determined by both nuclear and mitochondrial genes. In maize C-type cytoplasmic male sterility, it is unclear how the development of meiocytes and microspores is affected by the mitochondrial sterility gene and the nuclear restorer gene. In this study, we sequenced the transcriptomes of single meiocytes(tetrad stage) and early mononucleate microspores from sterile and restorer lines. The numbers of expressed genes varied in individual cells and fewer than half of the expressed genes were common to the same cell types. Four comparisons revealed 3379 differentially expressed genes(DEGs), with 277 putatively associated with mitochondria, 226 encoding transcription factors,and 467 possibly targeted by RF4. KEGG analysis indicated that the DEGs in the two lines at the tetrad stage were involved predominantly in carbon metabolism and in amino acid biosynthesis and metabolism, whereas the DEGs during the transition from the tetrad stage to the early mononucleate stage were associated mostly with regulation of protein metabolism, fatty acid metabolism, and anatomical structure morphogenesis. Thus, meiocyte and microspore development was affected by the surrounding cells and the restorer gene, and the restorer gene helped restore the redox homeostasis of microspores and the normal cellular reconstruction during the transition.

关键词： Maize Single-cell sequencing Cytoplasmic male sterility Restoration Heterosis

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马钢CSP工序过程磷的控制

钢铁 2008年第10期43卷 86-90页

作者：丁长江周俐范鼎东安徽工业大学冶金与资源学院安徽马鞍山243000 马钢股份有限公司第一钢轧总厂安徽马鞍山243000

通过控制转炉工艺原辅材料质量,改进和优化加料、供氧等吹炼模型,实现了高的转炉吹炼终点[C]、T命中率;摸索出全程跟踪化渣技术,保证了吹炼过程的稳定性;提高挡渣效果,使大罐内渣层厚度由原来的100 mm以上降到50 mm左右。通过工艺优化... 详细信息

通过控制转炉工艺原辅材料质量,改进和优化加料、供氧等吹炼模型,实现了高的转炉吹炼终点[C]、T命中率;摸索出全程跟踪化渣技术,保证了吹炼过程的稳定性;提高挡渣效果,使大罐内渣层厚度由原来的100 mm以上降到50 mm左右。通过工艺优化提高了马钢CSP工序的脱磷效果,转炉的脱磷率提高到93%以上,终点磷的质量分数平均为0.008 8%,过程总回磷小于0.005%,成品平均磷的质量分数为0.010%,提高了产品的质量。

关键词： CSP工序磷含量控制