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Global Trends in Dam Removal and Related Research: A Systematic Review Based on Associated Datasets and Bibliometric Analysis

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Chinese Geographical Science 2019年第1期29卷 1-12页

作者： ding Liuyong CHEN Liqiang ding chengzhi TAO Juan Yunnan Key Laboratory of International Rivers and Transboundary Eco-security Yunnan University Institute of International Rivers and Eco-security Yunnan University

Dam removal has been increasingly reported globally and is becoming an important approach for river management, restoration and environmental conservation in damming rivers. However, current limited knowledge of global trends in dam removal and related research may be potentially biased in terms of the geographic distribution and organisms studied. Such bias could mislead dam removal planning and management in different areas and ecological conservation for different taxa. In this study, we quantitatively and qualitatively analyzed datasets of dam removal and publications of dam removal research using bibliometric methods. A total of 1449 dam removal documents were published from 1953 to 2016. Trends, current hotspots and future directions of dam removal research were identified. The results from this study reveal that dam removal largely occurred in the North America and Europe, and most of the removed dams were small and old dams. With respect to the topic analysis, more dam removal studies should focus on the responses of a wide range of organisms, not only fish, as well as the interspecies relationships, food webs and ecosystem structures and functions. Based on our findings, we also provide some suggestions for future dam removal planning and analysis.

关键词： dam demolition literature research topics weir

circDDX17靶向miR-223-3p/RIP3调控非小细胞肺癌细胞增殖及凋亡

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中华肿瘤杂志 2024年第3期46卷 239-248页

作者：丁成智王国磊姜功前王洪涛刘源渊张浩亮孙芳魏立河南省人民医院、郑州大学人民医院胸外科郑州450003 河南省胸科医院胸外科郑州450008 河南省胸科医院呼吸内科郑州450008

目的探讨circDDX17通过靶向miR-223-3p/RIP3分子轴调控非小细胞肺癌细胞增殖及凋亡的分子机制。方法采用实时荧光定量聚合酶链反应(qRT-PCR)检测人正常肺上皮细胞株BEAS-2B和非小细胞肺癌H1299、A549、H446细胞中circDDX17、miR-223-3p... 详细信息

目的探讨circDDX17通过靶向miR-223-3p/RIP3分子轴调控非小细胞肺癌细胞增殖及凋亡的分子机制。方法采用实时荧光定量聚合酶链反应(qRT-PCR)检测人正常肺上皮细胞株BEAS-2B和非小细胞肺癌H1299、A549、H446细胞中circDDX17、miR-223-3p、受体相互作用蛋白3(RIP3)的表达水平。分别将pcDNA、pcDNA-circDDX17、anti-miR-con、anti-miR-223-3p、pcDNA-circDDX17与miR-con、pcDNA-circDDX17与miR-223-3p mimics转染至H1299细胞。采用四甲基偶氮唑蓝法检测细胞增殖情况,流式细胞术检测细胞周期和细胞凋亡情况,平板克隆实验检测细胞增殖能力,双荧光素酶报告实验验证circDDX17与miR-223-3p、miR-223-3p与RIP3的靶向关系,Western blot法检测CyclinD1、CDK2、Cleaved caspase-3、Bax蛋白的表达水平。结果与BEAS-2B比较,H1299、A549、H446细胞中circDDX17 mRNA表达水平降低,miR-223-3p mRNA表达水平升高,RIP3 mRNA的表达水平降低(均P<0.05)。pcDNA-circDDX17组细胞活力[(69.46±4.68)%]、细胞克隆数[(83.49±7.86)个]、S期细胞比例[(22.52±1.41)%]、CyclinD1、CDK2蛋白表达水平均低于pcDNA组[分别为(97.54±7.72)%、(205.03±13.37)个、(28.69±1.49)%,均P<0.05],pcDNA-circDDX17组细胞G0/G1期细胞比例[(64.45±3.56)%]、细胞凋亡率[(18.36±1.63)%]、Cleaved caspase-3、Bax蛋白表达水平均高于pcDNA组[分别为(51.33±2.76)%和(5.21±0.54)%,均P<0.05];anti-miR-223-3p组细胞活力[(72.64±5.44)%]、细胞克隆数[(78.16±8.23)个]、S期细胞比例[(21.34±1.59)%]、CyclinD1、CDK2蛋白表达水平均低于anti-miR-con组[分别为103.47±6.25、(169.32±14.53)个、(28.43±1.26)%,均P<0.05],anti-miR-223-3p组细胞G0/G1期细胞比例[(62.86±3.28)%]、细胞凋亡率[(14.64±1.67)%]、Cleaved caspase-3、Bax蛋白表达水平均高于anti-miR-con组[分别为(51.33±2.71)%和(4.83±0.39)%,均P<0.05]。circDDX17与miR-223-3p存在结合位点,miR-223-3p与RIP3存在结合位点。pcDNA-circDDX17+miR-223-3p组细胞活力[(135.45±9.28)%]、细胞克隆数[(174.64±10.68)个]、S期细胞比例[(26.39±2.25)%]、CyclinD1、CDK2蛋白表达水平均高于pcDNA-circDDX17+miR-con组[分别为(101.56±6.68)%、(107.65±7.62)个、(21.64±1.72)%,均P<0.05],pcDNA-circDDX17+miR-223-3p组细胞G_(0)/G_(1)期细胞比例[(56.64±2.76)%]、细胞凋亡率[(8.34±0.76)%]、Cleaved caspase-3、Bax蛋白表达水平均低于pcDNA-circDDX17+miR-con组[分别为(64.03±3.48)%和(15.21±1.18)%,均P<0.05]。结论circDDX17可靶向调控miR-223-3p/RIP3分子轴从而抑制非小细胞肺癌细胞增殖及诱导细胞凋亡。

关键词：肺肿瘤 circDDX17 miR-223-3p 受体相互作用蛋白3 增殖凋亡

Analytical model for predicting time-dependent lateral deformation of geosynthetics-reinforced soil walls with modular block facing

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第2期16卷 711-725页

作者： Luqiang ding chengzhi Xiao Feilong Cui School of Civil and Transportation Engineering Hebei University of TechnologyTianjin300401China

To date,few models are available in the literature to consider the creep behavior of geosynthetics when predicting the lateral deformation(d)of geosynthetics-reinforced soil(GRS)retaining *** this study,a general hyperbolic creep model was first introduced to describe the long-term deformation of geosynthetics,which is a function of elapsed time and two empirical parameters a and *** conventional creep tests with three different tensile loads(Pr)were conducted on two uniaxial geogrids to determine their creep behavior,as well as the a-Pr and b-Pr *** test results show that increasing Pr accelerates the development of creep deformation for both ***,a and b respectively show exponential and negatively linear relationships with Pr,which were confirmed by abundant experimental data available in other *** on the above creep model and relationships,an accurate and reliable analytical model was then proposed for predicting the time-dependent d of GRS walls with modular block facing,which was further validated using a relevant numerical investigation from the previous *** evaluation and comparison of the proposed model with six available prediction models were *** a parametric study was carried out to evaluate the effects of wall height,vertical spacing of geogrids,unit weight and internal friction angle of backfills,and factor of safety against pullout on d at the end of construction and 5 years *** findings show that the creep effect not only promotes d but also raises the elevation of the maximum d along the wall ***,the limitations and application prospects of the proposed model were discussed and analyzed.

关键词： Geosynthetics Creep behavior Geosynthetics-reinforced soil(GRS)walls Lateral deformation Analytical model

牛病毒性腹泻病毒E^(rns)-ELISA抗体检测试剂盒的制备

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中国兽医杂志 2024年第7期60卷 65-70页

作者：王静陈柯源王胜华丁成志杨光辉刘一尹金花王九峰中国农业大学动物医学院北京海淀100193 中国动物疾病预防控制中心北京大兴102618 塔里木大学动物科学与技术学院新疆阿拉尔843300

为建立快速检测牛病毒性腹泻病毒(BVDV)的血清学方法,本试验以BVDV NADL毒株结构蛋白E^(rns)作为包被抗原,优化反应条件并组装BVDV酶联免疫吸附试验(ELISA)抗体检测试剂盒,通过重复性试验、灵敏性试验、符合率试验和保存期试验验证该试... 详细信息

为建立快速检测牛病毒性腹泻病毒(BVDV)的血清学方法,本试验以BVDV NADL毒株结构蛋白E^(rns)作为包被抗原,优化反应条件并组装BVDV酶联免疫吸附试验(ELISA)抗体检测试剂盒,通过重复性试验、灵敏性试验、符合率试验和保存期试验验证该试剂盒的准确性、灵敏性和稳定性。结果显示,抗原包被浓度为4μg/mL,1%酪蛋白溶液37℃封闭45 min,被检血清以1∶400稀释孵育30 min,兔抗牛HRP标记二抗以1∶20000稀释孵育30 min,TMB底物反应5 min时,ELISA的反应背景下降,区分度最好。优化后方法的批内重复性试验和批间重复性试验的变异系数均在10%以内,表明该试剂盒具有较好的稳定性;灵敏性试验结果显示,当阳性血清稀释度达到1∶6400时仍可以检测为阳性,说明该试剂盒灵敏度较高;该试剂盒与美国爱德士生物科技公司(IDEXX)BVDV总抗体检测试剂盒共同检测466份临床血清样品,两者总符合率为89.1%;保存期试验结果显示,第4个月时该试剂盒仍能检测到阳性血清,说明稳定性较好。综上表明,本试验利用E^(rns)蛋白建立的BVDV间接ELISA抗体检测试剂盒灵敏度高且重复性好,可为BVDV抗体检测和流行性调查提供新的方法。

关键词：牛病毒性腹泻病毒 E^(rns)蛋白抗体间接ELISA

Fish Assemblage Responses to a Low-head Dam Removal in the Lancang River

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Chinese Geographical Science 2019年第1期29卷 26-36页

作者： ding chengzhi JIANG Xiaoming WANG Lieen FAN Hui CHEN Liqiang HU Jinming WANG Hailong CHEN Yifeng SHI Xiaotao CHEN Hao PAN Bohui ding Liuyong ZHANG Chao HE Daming Yunnan Key Laboratory of International Rivers and Transboundary Eco-security Yunnan University State Key Laboratory of Eco-hydraulic in Northwest Arid Region of China Xi'an University of Technology Huaneng Lancang River Hydropower Co. Ltd Laboratory of Biological Invasion and Adaptive Evolution Institute of Hydrobiology Chinese Academy of Sciences Engineering Research Center of Eco-environment in Three Gorges Reservoir Region Ministry of Education China Three Gorges University

Dam removal is becoming an effective approach for aquatic biodiversity restoration in damming river in order to balance the aquatic ecosystem conservation with large-scale cascade damming. However, the effects of dam removal on fish communities in Asian mountainous rivers, which are dominated by Cypriniformes fishes, are still not well known. To determine whether dam removal on a mountainous river benefit restoration of fish diversity, we investigated the response of fish assemblage to dam removal using a before-after-control-impact design in two tributaries of the Lancang River(dam removal river: the Jidu River, and control river: the Fengdian River). Fish surveys were conducted one year prior to dam removal(2012) and three years(2013–2015) following dam removal. We observed rapidly and notably spatio-temporal changes in fish biodiversity metrics and assemblage structure, occurring in the Jidu River within the first year after dam removal. Overall, fish species richness, density and Shannon-Wiener diversity all increased immediately in above-and below-dam sites, and maintained a stable level in subsequent years, compared to unchanged situation in the control river. All sites in the Jidu River experienced shifts in fish composition after dam removal, with the greatest temporal changes occurred in sites below-and above-the former dam, resulting in a temporal homogenization tendency in the dam removed river. These findings suggest that dam removal can benefit the recovery of habitat conditions and fish community in Asian mountainous rivers, but the results should be further evaluated when apply to other dammed rivers since the dam age, fluvial geomorphology and situation of source populations could all affect the responses of fish assemblages.

关键词： fish diversity dam demolition habitat recovery Cypriniformes international rivers

Clinical characteristics of human infection with a novel avian-origin influenza A（H10N8） virus

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Chinese Medical Journal 2014年第18期127卷 3238-3242页

作者： Zhang Wei Wan Jianguo Qian Kejian Liu Xiaoqing Xiao Zuke Sun Jian Zeng Zhenguo Wang Qi Zhang Jinxiang Jiang Guanghui Nie Cheng Jiang Rong ding chengzhi Li Ran Horby Peter Gao Zhancheng Nanchang University First Affiliated Hospital Nanchang Jiangxi 330006 China Nanchang First Hospital Nanchang Jiangxi 330008 China Jiangxi Province Center for Disease Control and Prevention Nanchang Jiangxi 330038 China Jiangxi Provincial People's Hospital Nanchang Jiangxi 330006China Nanchang University Fourth Affiliated Hospital Nanchang Jiangxi 330003 China Chinese Center for Disease Control and Prevention Beijing 102206China Department of Respiratory and Critical Care Medicine PekingUniversity People's Hospital Beijing 100044 China Oxford University Clinical Research Unit -Wellcome Trust MajorOverseas Programme Hanoi Viemam

Background Novel influenza A viruses of avian-origin may be the precursors of pandemic strains. This descriptive study aims to introduce a novel avian-origin influenza A （H10N8） virus which can infect humans and cause severe diseases. Methods Collecting clinical data of three cases of human infection with a novel reassortment avian influenza A （H10N8） virus in Nanchang, Jiangxi Province, China. Results Three cases of human infection with a new reassortment avian influenza A（H10N8） virus were described, of which two were fatal cases, and one was severe case. These cases presented with severe pneumonia that progressed to acute respiratory distress syndrome （ARDS） and intractable respiratory failure. Conclusion This novel reassortment avian influenza A （H10N8） virus in China resulted in fatal human infections, and should be added to concerns in clinical practice.

关键词： human infection avian influenza A(H10N8) virus reassortment clinical characteristics

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怒江裂腹鱼鳃的显微和超微结构

中国水产科学 2018年第6期25卷 1183-1193页

作者：向乾乾丁刘勇张超孙洁王迪丁城志陈礼强云南大学国际河流与生态安全研究院云南省国际河流与跨境生态安全重点实验室

通过光镜、扫描电镜及透射电镜技术,研究了怒江裂腹鱼(Schizothorax nukiangensis)鳃的组织结构和超微结构。结果表明,怒江裂腹鱼鳃基本结构与其他硬骨鱼相似,均由鳃耙、鳃弓、鳃丝以及鳃小片构成,且各部分具有不同的组织结构和细胞类... 详细信息

通过光镜、扫描电镜及透射电镜技术,研究了怒江裂腹鱼(Schizothorax nukiangensis)鳃的组织结构和超微结构。结果表明,怒江裂腹鱼鳃基本结构与其他硬骨鱼相似,均由鳃耙、鳃弓、鳃丝以及鳃小片构成,且各部分具有不同的组织结构和细胞类型。扁平上皮细胞覆盖于整个鳃表面,其表面的纹路主要有3种类型,在鳃上不同的部位具有不同的结构和功能。线粒体密集细胞主要分布于鳃小片基部,仅有一种细胞类型,顶端膜内凹呈浅滩型,细胞内具有大量圆形的线粒体。怒江裂腹鱼鳃内扁平上皮细胞和线粒体密集细胞的形态结构与其在急流中游泳和摄食活动等生活习性相适应。本研究结果丰富了怒江裂腹鱼的基础生物学资料,并为阐明鱼类鳃的结构特征与其生活习性之间的关系提供参考。

关键词：怒江裂腹鱼鳃扁平上皮细胞线粒体密集细胞组织结构超微结构

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怒江流域鱼类多样性及空间分布格局

生物多样性 2022年第7期30卷 117-129页

作者：杨科丁城志陈小勇丁刘勇黄敏睿陈晋南陶捐云南大学国际河流与生态安全研究院昆明650500 云南省国际河流与跨境生态安全重点实验室昆明650500 中国科学院昆明动物研究所遗传资源与进化国家重点实验室昆明650223

怒江是我国西南地区重要的国际河流,也是全球生物多样性热点区域之一。受地理位置和地形条件限制,目前尚缺乏怒江流域鱼类物种多样性及其分布格局的系统研究。本研究利用近5年的全流域实地采样数据,结合文献资料,系统整理了怒江流域鱼... 详细信息

怒江是我国西南地区重要的国际河流,也是全球生物多样性热点区域之一。受地理位置和地形条件限制,目前尚缺乏怒江流域鱼类物种多样性及其分布格局的系统研究。本研究利用近5年的全流域实地采样数据,结合文献资料,系统整理了怒江流域鱼类物种组成信息;利用聚类和排序方法分析了怒江流域鱼类空间分布格局。结果表明,怒江流域共有土著鱼类85种,隶属于5目12科47属;外来鱼类18种,隶属于3目8科16属,另外实地调查发现新记录外来种2种。怒江流域鱼类多样性从上游至下游呈明显的递增趋势。根据土著鱼类组成的聚类和排序分析结果,可以将怒江25个亚流域分为3个部分:(1)西藏自治区内的上游河段,分布有鱼类15种,组成以裂腹鱼类、高原鳅类为主,具有明显青藏高原冷水性鱼类区系特征。(2)贡山至泸水的中游河段,分布有鱼类36种,组成兼有上游冷水性鱼类和下游喜温的鲃亚科、野鲮亚科鱼类。(3)泸水以下的下游河段,分布有鱼类74种。下游河段干流广泛分布有喜温的鲃亚科、野鲮亚科和亚科鱼类,支流则分布有数量众多的南鳅属(Schistura)、纹胸鮡属(Glyptothorax)鱼类,鱼类组成以适应流水、激流环境的热带亚热带鱼类为主。2017–2021年的多次调查仅发现土著鱼类43种(占历史记录的50.6%),土著鱼类资源衰退明显。过度捕捞、支流小水电开发、外来鱼类入侵等是主要威胁因素。随着人类活动的急剧增多,怒江流域鱼类将面临更大的威胁,亟需从流域层面开展系统的鱼类资源管理和保护规划。

关键词：鱼类物种丰富度多样性格局相似性分析怒江国际河流

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淡水鱼类功能生态学研究进展

生态学报 2019年第3期39卷 810-822页

作者：程馨雨陶捐武瑞东陈礼强丁城志云南大学国际河流与生态安全研究院昆明650091 云南大学云南省国际河流与跨境生态安全重点实验室昆明650091

在全球变化和人类活动的影响下,生物多样性正以前所未有的速度丧失,全球生物正经受第六次生物多样性危机。淡水生态系统是最脆弱的生态系统之一。淡水鱼类作为淡水生态系统的重要组成部分,承受着日趋严重的气候变化、栖息地退化、生物... 详细信息

在全球变化和人类活动的影响下,生物多样性正以前所未有的速度丧失,全球生物正经受第六次生物多样性危机。淡水生态系统是最脆弱的生态系统之一。淡水鱼类作为淡水生态系统的重要组成部分,承受着日趋严重的气候变化、栖息地退化、生物入侵和过度捕捞等压力,面临巨大的威胁。在此背景下,如何准确评估鱼类种群和群落对环境变化的响应,以及鱼类群落结构和功能的变化对生态系统功能的影响是淡水鱼类多样性和淡水生态系统保护的关键问题。近年来,淡水鱼类功能生态学的快速发展为解答这一问题提供了一个框架。系统地介绍了淡水鱼类功能生态学主要研究内容、方法、进展及其应用,并着重介绍了淡水鱼类功能特征及其与环境的关系、环境变化下的功能生态学响应研究。据此提出了淡水鱼类功能生态学未来的重点研究方向,指出了其在鱼类多样性保护和资源利用等领域的应用前景。

关键词：淡水鱼类功能生态学功能特征生物多样性