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Ink-structing the future of vascular tissue engineering:a review of the physiological bioink design

Bio-Design and Manufacturing 2024年第2期7卷 181-205页

作者： Judith Synofzik Sebastian Heene Rebecca Jonczyk cornelia Blume Institute for Technical chemistry Leibniz University Hannover30167 HannoverGermany

Three-dimensional(3D)printing and bioprinting have come into view for a plannable and standardizable generation of implantable tissue-engineered constructs that can substitute native tissues and *** tissue-engineered structures are intended to integrate with the patient’s *** tissue engineering(TE)is relevant in TE because it supports the sustained oxygenization and nutrition of all tissue-engineered *** have a specific role,representingthenecessarymedium for printability and vascular cell *** review aims to understand the requirements for the design of vascular ***,an in-depth analysis of vascular cell interaction with their native environment must be gained.A physiological bioink suitable for a tissue-engineered vascular graft(TEVG)must not only ensure good printability but also induce cells to behave like in a native vascular vessel,including self-regenerative and growth *** review describes the general structure of vascular walls with wall-speciﬁc cell and extracellular matrix(EcM)components and biomechanical properties and ***,the physiological role of vascular EcM components for their interaction with vascular cells and the mode of interaction is *** currently available or imaginable bioinks are described from physiological matrix proteins to nonphysiologically occurring but natural chemical compounds useful for vascular *** physiological performance of these bioinks is evaluated with regard to biomechanical properties postprinting,with a view to current animal studies of 3D printed vascular ***,the main challenges for further bioink development,suitable bioink components to create a self-assembly bioink concept,and future bioprinting strategies are *** concepts are discussed in terms of their suitability to be part of a TEVG with a high potential for later clinical use.

关键词： Vascular wall histology Vascular cells Microenvironment Extracellular matrix cell–matrix interaction Bioink Printability

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Growth efficiency,intestinal biology,and nutrient utilization and requirements of black soldier fly(Hermetia illucens)larvae compared to monogastric livestock species:a review

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1262-1281页

作者： Mohammad M.Seyedalmoosavi Manfred Mielenz Teun Veldkamp Gurbuz Das cornelia c.Metges Research Institute for Farm Animal Biology(FBN) Institute of Nutritional Physiology18196 DummerstorfGermany Wageningen UR Livestock ResearchP.O.Box 3386700AH WageningenNetherlands

In recent years,interest in the larvae of black soldier fly(BSF)(Hermetia illucens)as a sustainable protein resource for livestock feed has increased ***,knowledge on the nutritional and physiological aspects of this insect,especially compared to other conventional farmed animals is *** review presents a critical comparison of data on the growth potential and efficiency of the BSF larvae(BSFL)compared to conventional monogastric livestock *** of BSFL over other monogastric livestock species includes their high growth rate and their ability to convert low-grade organic waste into high-quality protein and fat-rich biomass suitable for use in animal *** using literature data suggest that BSFL are more efficient than broilers,pigs and fish in terms of conversion of substrate protein into body mass,but less efficient than broilers and fish in utilization of substrate gross energy to gain body *** growth efficiency varies greatly depending on the nutrient quality of their dietary *** might be associated with the function of their gastrointestinal tract,including the activity of digestive enzymes,the substrate particle characteristics,and their intestinal microbial *** conceived advantage of BSFL having an environmental footprint better than conventional livestock is only true if BSFL is produced on low-grade organic waste and its protein would directly be used for human ***,their potential role as a new species to better close nutrient cycles in agro-ecological systems needs to be reconsidered,and we conclude that BSFL is a complementary livestock species efficiently utilizing organic waste that cannot be utilized by other *** addition,we provide comparative insight into morpho-functional aspects of the gut,characterization of digestive enzymes,gut microbiota and fiber ***,current knowledge on the nutritional utilization and requirements of BSFL in te

关键词： Black soldier fly comparative growth performance Farm animals Insects as feed Intestinal function Nutrient cycles Nutrition and physiology

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Arabidopsis FHY1 and FHY1-LIKE Are Not Required for Phytochrome A Signal Transduction in the Nucleus

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Plant communications 2020年第2期1卷 34-44页

作者： chiara Menon cornelia Klose Andreas Hiltbrunner center for Plant Molecular Biology University of Tubingen72076 TubingenGermany Faculty of Biology Institute of Biology IIUniversity of Freiburg79104 FreiburgGermany Signalling Research centres BIOSS and cIBSS University of Freiburg79104 FreiburgGermany

Photoreceptors of the phytochrome family control a multitude of responses in *** A(phyA)is essential for far-red light perception,which is important for germination and seedling establishment in strong canopy *** of phyA from the cytosol into nucleus is a key step in farred light signaling and requires FAR-RED ELONGATED HYPOcOTYL 1(FHY1)and FHY1-LIKE(FHL).FHY1/FHL bind to phyA downstream signaling ***,it has been suggested that FHY1/FHL also have a function in assembling phyA transcription factor complexes in the ***,in this study,we show that constitutively nuclear-localized phyA is active in the absence of FHY1 and ***,an artificial FHY1,consisting of an SV40 NLS,a phyA binding site,and a YFP tag as spacer between them,complements the fhy1-3 fhl-1 double *** findings show that FHY1 and FHL are not required for phyA downstream signaling in the ***,we found that lines expressing phyA-NLS-YFP are hypersensitive to red and far-red light and that slightly increased levels of constitutively nuclear-localized phyA result in photomorphogenic development in the ***,restricting phyA to the cytosol and inducing nuclear transport in light by interaction with FHY1/FHL might be important to suppress photomorphogenesis in the dark.

关键词： phytochrome phyA FHY1 FHL nuclear transport far-red light

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Formulation of carbon black-ionomer dispersions for thin film formation in fuel cells

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Particuology 2019年第3期17卷 7-21页

作者： Eva Hoffmann Su Zhang Martin Thoma cornelia Damm Wolfgang Peukert Institute of Particle Technology (LFG) Friedrich-Alexander-Universitat Erlangen-Niimberg (FAU) CauerstraJSe 4 D-91058 Erlangen Germany

The performance of proton exchange membrane fuel cells (PEMFc) is strongly determined by the structure and composition of the electrode *** interactions between the ionomer,carbon black particles,and solvent affect the suspension properties and thus the layer *** analyze the effect of the ionomer-to-carbon (I/c) weight ratio for two different types of carbon black on the suspension and layer *** branched carbon blacks with a high surface area tend to form less cracked *** less branched carbons can pack together more closely,a smaller pore size results in a larger capillary pressure during drying and thus more *** added ionomer adsorbs on the carbon particles and improves the colloidal stability of the carbon black *** carbon black aggregates are thus smaller,resulting in closer packing and thinner ***,the addition of the ionomer increases the critical coating thickness (ccT) of the layers because drying stresses are dissipated by the deformation of the ionomer,preventing crack *** optimum I/c weight ratio is identified for optimal layer formation and minimized crack formation.

关键词： Perfluorosulfonic acid (PFSA) ionomer carbon black Layer formation cracking Porosity

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consensus views on competencies and teaching methods for an interprofessional curriculum on complementary and integrative medicine:A Delphi study

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Journal of Integrative Medicine 2021年第3期19卷 282-290页

作者： Angelika Homberg Katja Krug Nadja Klafke Katharina Glassen cornelia Mahler Svetla Loukanova Department of General Practice and Health Services Research University Hospital HeidelbergHeidelberg69120 HeidelbergGermany Department of Nursing Science University Hospital Tübingen72076 TubingenGermany

Objective:A collaborative team is necessary to help patients achieve their healthcare goals using complementary *** present,healthcare professionals do not feel sufficiently qualified to provide this *** study sought to identify competencies and teaching methods for interprofessional training on complementary and integrative medicine at medical ***:Sixty-five German-speaking experts with various professional backgrounds were invited to take part in a three-round Delphi *** the first round,predefined competencies were assessed on a seven-point Likert scale,and participants were invited to propose additional competencies that would be evaluated in the subsequent *** competencies were ranked based on the participant assessments and were assigned to four relevance *** the second and third rounds,suitable teaching methods were identified using free-text fields and multiple-choice *** a final workshop,participants synthesized the outcomes of the previous sessions and derived key competencies that would be a benefit to undergraduate interprofessional training in complementary and integrative medicine at medical ***:The three rounds plus final worksop were attended by 50,40,36 and 11 *** competencies that these experts determined to be highly relevant to teaching complementary and integrative medicine emphasized,in particular,the respectful treatment of patients and the importance of taking a medical *** these highly relevant competencies,three key targets were agreed upon in the final workshop:students are able to 1)classify and assess complementary medical terms and methods;2)work collaboratively and integrate patients into the interprofessional team;3)involve patients and their relatives respectfully and empathetically in all healthcare *** achieve these competency goals,the following teaching methods were highlighted:students discuss therapy options based on authentic patient c

关键词： Undergraduate education Interprofessional education complementary medicine Delphi study curriculum competency

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Determination of quantitative structure-property and structure-process relationships for graphene production in water

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Nano Research 2015年第6期8卷 1865-1881页

作者： Thomas J. Nacken cornelia Damm Haichen Xing Andreas ROger Wolfgang Peukert Institute of Particle Technology (LFG) Friedrich-Alexander University Erlangen-NOrnberg (FAU) Cauerstrasse 4 91058 Erlangen Germany

A scalable method for graphene and few-layer graphene （FLG） production by graphite delamination in aqueous solutions of the nonionic surfactant TWEEN 80 （TW80） using stirred-media mills is presented. Delaminated product analysis using statistical Raman spectroscopy yielded extensive processing-structure-property relationships that revealed how stress intensity and specific energy input, i.e., the process parameters, govern the yield of graphene production and defect formation. The dispersed carbon concentration increased but the content and the quality of the FLG product decreased sharply with higher specific energy input. The FLG content of the product was up to 90%, especially for low specific energy input. Moreover, Raman analyses revealed that stress intensities greater than about I nJ were related to significant defect formation in the product particles. Another key parameter for graphene production is solvent viscosity. The FLG concentration in the product increased by a factor of 10 when the solvent＇s viscosity was increased from 1 to 6 mPa-s because shear- and friction-induced delamination was enhanced and in-plane fracture was reduced due to dampening of bead motion. Based on the processing-structure-property relationships found, we propose that the delamination process can be designed in such way that the product consists, almost totally, of FLG and that single-layer graphene is produced. The scalability of graphene production by stirred-media delamination was demonstrated when an increase in the batch size from 0.2 to 2 L had no significant effect on product quality.

关键词： graphite delamination few-layer graphene graphene stirred-media millprocess parameters statistical Ramanspectroscopy

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Advances in greenhouse automation and controlled environment agriculture:A transition to plant factories and urban agriculture

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International Journal of Agricultural and Biological Engineering 2018年第1期11卷 1-22页

作者： Redmond Ramin Shamshiri Fatemeh Kalantari K.c.Ting Kelly R.Thorp Ibrahim A.Hameed cornelia Weltzien Desa Ahmad Zahra Mojgan Shad Smart Farming Technology Research center Department of Biological and Agricultural EngineeringFaculty of EngineeringUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Landscape Architecture Faculty of Design and ArchitectureUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Agricultural and Biological Engineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA United States Department of Agriculture Agricultural Research ServiceU.S.Arid-Land Agricultural Research CenterMaricopaAZ 85138USA Dept.of IcT and Natural Sciences Faculty of Information Technology and Electrical EngineeringNorwegian University of Science and Technology(NTNU)Larsgårdsveien 2NO-6009ÅlesundNorway Leibniz Institute for Agricultural Engineering and Bioeconomy Max-Eyth-Allee 10014469 Potsdam-BornimGermany Adaptive AgroTech consultancy International CA 93955USA

Greenhouse cultivation has evolved from simple covered rows of open-fields crops to highly sophisticated controlled environment agriculture(cEA)facilities that projected the image of plant factories for urban *** advances and improvements in cEA have promoted the scientific solutions for the efficient production of plants in populated cities and multi-story *** deployment of cEA for urban agriculture requires many components and subsystems,as well as the understanding of the external influencing factors that should be systematically considered and *** review is an attempt to highlight some of the most recent advances in greenhouse technology and cEA in order to raise the awareness for technology transfer and adaptation,which is necessary for a successful transition to urban *** study reviewed several aspects of a high-tech cEA system including improvements in the frame and covering materials,environment perception and data sharing,and advanced microclimate control and energy optimization *** research highlighted urban agriculture and its derivatives,including vertical farming,rooftop greenhouses and plant factories which are the extensions of cEA and have emerged as a response to the growing population,environmental degradation,and urbanization that are threatening food ***,several opportunities and challenges have been identified in implementing the integrated cEA and vertical farming for urban agriculture.

关键词： smart agriculture greenhouse modelling urban agriculture vertical farming automation internet of things(IoT) wireless sensor network plant factories

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Machine learning-based spectral and spatial analysis of hyper-and multi-spectral leaf images for Dutch elm disease detection and resistance screening

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Artificial Intelligence in Agriculture 2023年第4期10卷 26-34页

作者： Xing Wei Jinnuo Zhang Anna O.conrad charles E.Flower cornelia c.Pinchot Nancy Hayes-Plazolles Ziling chen Zhihang Song Songlin Fei Jian Jin Department of Agricultural and Biological Engineering Purdue UniversityWest LafayetteIN 47907United States USDA Forest Service Northern Research StationHardwood Tree Improvement and Regeneration CenterWest LafayetteIN 47906United States USDA Forest Service Northern Research StationDelawareOH 43015United States Department of Forestry and Natural Resources Purdue UniversityWest LafayetteIN 47907United States

Diseases caused by invasive pathogens are an increasing threat to forest health,and early and accurate disease detection is essential for timely and precision forest *** recent technological advancements in spectral imaging and artificial intelligence have opened up new possibilities for plant disease detection in both crops and *** this study,Dutch elm disease(DED;caused by Ophiostoma novo-ulmi,)and American elm(Ulmus americana)was used as example pathosystem to evaluate the accuracy of two in-house developed high-precision portable hyper-and multi-spectral leaf imagers combined with machine learning as new tools for forest disease ***-and multi-spectral images were collected from leaves of American elm geno-types with varied disease susceptibilities after mock-inoculation and inoculation with ***-ulmi under green-house *** traditional machine learning and state-of-art deep learning models were built upon derived spectra and directly upon spectral image *** learning models that incorporate both spectral and spatial features of high-resolution spectral leaf images have better performance than traditional machine learning models built upon spectral features alone in detecting *** and symptomatic spots on the leaves were highlighted in the deep learning model as important spatial features to distinguish leaves from inoculated and mock-inoculated *** addition,spectral and spatial feature patterns identified in the machine learning-based models were found relative to the DED susceptibility of elm *** further studies are needed to assess applications in other pathosystems,hyper-and multi-spectral leaf imagers combined with machine learning show potential as new tools for disease phenotyping in trees.

关键词： American elm Dutch elm disease Hyperspectral imaging Multispectral imaging Support vector machine convolution neural network Disease phenotyping Digital forestry

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Examining enablers of vaccine hesitancy toward routine childhood and adolescent vaccination in Malawi

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Global Health Research and Policy 2022年第1期7卷 232-241页

作者： Gbadebo collins Adeyanju cornelia Betsch Abdu AAdamu Khadijah Sanusi Gumbi Michael GHead Aristide Aplogan Haoua Tall Tene-Alima Essoh Media and communication Science University of ErfurtErfurtGermany center for Empirical Research in Economics and Behavioural Science(cEREB) University of ErfurtErfurtGermany Bernard Nocht Institute of Tropical Medicine(BNITM) HamburgGermany cochrane South Africa South African Medical Research CouncilCape TownSouth Africa Division of Epidemiology and Biostatistics Department of Global HealthFaculty of Medicine and Health SciencesStellenbosch UniversityCape TownSouth Africa Ahmadu Bello University ZariaKadunaNigeria clinical Informatics Research Unit Faculty of MedicineUniversity of SouthamptonSouthamptonUK Agence de Médecine Préventive Regional Directorate for AfricaAbidjanCôte d’Ivoire

Background:The contribution of vaccination to global public health and community wellbeing has been described as one of the greatest success stories of modern ***,13.5 million children still miss at least one of their routine vaccinations,and this contributes to about 1.5 million deaths from vaccine-preventable *** of the contributing factors has been associated with vaccine *** hesitancy is the delay or refusal of vaccines despite their *** study explored factors from multiple perspectives that influence hesitancy among caregivers of children and adolescent girls eligible for childhood routine immunisation and the Human Papillomavirus vaccine in ***:The methodology used was qualitative such as key informant interviews and focus-group *** was obtained from caregivers,community and religious leaders,leaders of civil society groups,teachers in schools where Human Papillomavirus vaccine were piloted,healthcare workers,national and district-level officials of the expanded program on *** were 25 key informant interviews and two focus-group discussions,with 13 *** study was conducted between April to May *** Interviews and discussions were audio-recorded,transcribed,and analysed using a thematic content ***:Most vaccine-hesitancy drivers for routine immunisation were also relevant for the HPV *** drivers included inadequate awareness of the vaccination schedule,rumours and conspiracy theories exacerbated by religious beliefs,low literacy levels of caregivers,distance and transport to the vaccination clinic,gender role and a disconnect between community healthcare workers and community ***:The study demonstrated that a network of factors determines vaccine hesitancy for childhood Routine Immunisation and Human Papillomavirus,and some of them are interrelated with one *** has implications both for current levels of vac

关键词： HPV Malawi Routine immunisation Vaccination Vaccine hesitancy Vaccine uptake childhood Adolescent

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Differential cross Sections and collision-Induced Rotational Alignment in Inelastic Scattering of NO(X)by Xe

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chinese Journal of chemical Physics 2020年第2期33卷 217-233,I0003,J0001-J0017页

作者： Mark Brouard Helen chadwick Sean DSGordon cornelia GHeid Balazs Hornung Bethan Nichols Jacek Klos Pablo GJambrina FJavier Aoiz The Department of chemistry University of OxfordThe Chemistry Research Laboratory12 Mans-field RoadOxford OX13TAUK Department of chemistry and Biochemistry University of MarylandCollege ParkMaryland 20742USA Departamento de Química Física Facultad de Ciencias QuímicasUniversity of SalamancaSala-mancaSpain Departamento de Química Física Facultad de QuímicaUniversidad Complutense28040 MadridSpain

Fully A-doublet resolved differential cross sections and collision-induced rotational alignment moments have been measured for the NO(X)-Xe collision system at a collision energy of 519 cm^-*** experiments combine initial quantum state selection,employing a hexapole inhomogeneous electric field,with quantum state resolved detection,using(1+1')resonantly enhanced multiphoton ionization and velocity map ion *** differential cross sections and polarization dependent differential cross sections are shown to agree well with quantum mechanical scattering calculations performed on ab initio potential energy surfaces[*** et ***.137,014312(2012)].By comparison with quasi-classical trajectory calculations,quantum mechanical scattering calculations on a hard-shell potential,and kinematic apse model calculations,the effects of the attractive part of the potential on the measured differential cross sections and collision-induced rotational alignment moments are assessed.

关键词： chemical Physics Reaction dynamics Inelastic scattering

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