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De Novo Biosynthesis of Vindoline and Catharanthine in Saccharomyces cerevisiae

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BioDesign Research 2022年第1期2022卷 1-10页

作者： di gao Tengfei Liu Jucan gao Junhao Xu Yuanwei Gou Yingjia Pan Dongfang Li Cuifang Ye Ronghui Pan Lei Huang Zhinan Xu Jiazhang Lian Key Laboratory of Biomass Chemical Engineering of Ministry of Education College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200China Zhejiang Key Laboratory of Smart Biomaterials Zhejiang UniversityHangzhou 310027China.

Vinblastine has been used clinically as one of the most potent therapeutics for the treatment of several types of ***,the traditional plant extraction method suffers from unreliable supply,low abundance,and extremely high ***,we use synthetic biology approach to engineer Saccharomyces cerevisiae for de novo biosynthesis of vindoline and catharanthine,which can be coupled chemically or biologically to *** the basis of a platform strain with sufficient supply of precursors and cofactors for biosynthesis,we reconstituted,debottlenecked,and optimized the biosynthetic pathways for the production of vindoline and *** vindoline biosynthetic pathway represents one of the most complicated pathways ever reconstituted in microbial cell *** shake flask fermentation,our engineered yeast strains were able to produce catharanthine and vindoline at a titer of 527.1 and 305.1μg·liter^(−1),respectively,without accumulating detectable amount of pathway *** study establishes a representative example for the production of valuable plant natural products in yeast.

关键词： constitute engineer valuable

An NIR photothermal-responsive hybrid hydrogel for enhanced wound healing

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Bioactive Materials 2022年第10期7卷 162-172页

作者： Lin Jin Xiaoqing Guo di gao Yan Liu Jiahua Ni Zhiming Zhang Yiqiao Huang Guibin Xu Zhe Yang Xingcai Zhang Xianhan Jiang Department of Urology The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou510700PR China International Joint Research Laboratory for Biomedical Nanomaterials of Henan Zhoukou Normal UniversityZhoukou466001PR China The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’an710049PR China School of Engineering and Applied Sciences Harvard UniversityCambridgeMA02138USA School of Engineering Massachusetts Institute of TechnologyCambridgeMA02139USA Research Institute of Xi’an Jiaotong University HangzhouZhejiang311200PR China

Moderately regulating vascularization and immune microenvironment of wound site is necessary to achieve scarless wound healing of the ***,we have prepared an angiogenesis-promoting and scar-preventing band-aid with a core-shell structure,that consists of MXene-loaded nanofibers(MNFs)as the core and dopamine-hyaluronic acid hydrogel(H)as the shell(MNFs@V-H@DA)to encapsulate a growth factor(vascular endothelial growth factor,VEGF,abbreviated as V)and H2S donor(diallyl trisulfide,DATS,abbreviated as DA).The continuous release of DA from this system produced H2S,which would successfully induce macrophages to polarize into M2-lile phenotype,regulating the immune microenvironment and inhibiting an excessive inflammatory response at the wound *** is conducive to the proliferation of skin cells,facilitating the wound *** addition,an appropriate amount of VEGF can be released from the MXene nanofibrous skeleton by adjusting the time of near-infrared(NIR)light exposure,preventing excessive neovascularization and extracellular matrix deposition at the wound ***,this NIR photothermal-responsive band-aid achieved scarless wound healing through gradient-controlled vascularization and a related immune sequential reaction of damaged skin tissue.

关键词： Angiogenesis promotion NIR response Hydrogel Nanofibers Scarless wound healing

1,6-二氢-4-甲基-6-氧代-3-吡啶磺酰氯的合成工艺研究

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广东化工 2024年第3期51卷 1-3页

作者：周瑞周志旭岳朝晨张娅李磊高笛鹿田柴慧芳贵州中医药大学药学院贵州贵阳550025 贵州大学药学院贵州贵阳550025

1,6-二氢-4-甲基-6-氧代-3-吡啶磺酰氯是一种重要医药化工中间体。本文以4-甲基-5-硝基吡啶-2-醇为原料,经两步反应得到目标化合物。该合成方法操作简便、后处理操作简便,花费时间少且产物纯度高,产品收率为33.51%;此方法为1,6-二氢-4-... 详细信息

1,6-二氢-4-甲基-6-氧代-3-吡啶磺酰氯是一种重要医药化工中间体。本文以4-甲基-5-硝基吡啶-2-醇为原料,经两步反应得到目标化合物。该合成方法操作简便、后处理操作简便,花费时间少且产物纯度高,产品收率为33.51%;此方法为1,6-二氢-4-甲基-6-氧代-3-吡啶磺酰氯的合成提供了合成路线,也为进一步相关类似的合成研究奠定基础。

关键词： 4-甲基-5-硝基吡啶-2-醇 5-氨基-4-甲基吡啶-2-醇合成工艺医药

NaBH4-induced phase transition of CoSe_(2) with abundant Se deficiency for acidic oxygen reduction to hydrogen peroxide

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Rare Metals 2024年第2期43卷 500-510页

作者： Cai-di Han Yong-Chao Zhang Qian Zhang Jin-Ting Wu Jian gao Ji-Jun Zou Xiao-Dong Zhu State Key Laboratory Base of Eco-Chemical Engineering College of Chemical Engineering Qingdao University of Science and TechnologyQingdao266042China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China

Electrocatalytic oxygen reduction(ORR)via the 2e−pathway to form H_(2)O_(2) in acidic medium has attracted extensive ***,the low activity,insufficient selectivity and high cost of catalysts have been the ***,CoSe_(2) with abundant Se deficiency was synthesized by a simple hydrothermal method,and the addition of NaBH4-induced CoSe_(2) phase transition from orthorhombic to cubic phase with more Se *** cubic phase CoSe_(2) with abundant Se deficiency can effectively regulate the surface electronic structure with suitable binding energies of*OOH and*O,which shows high activity,selectivity and long-term stability for acidic ORR to H_(2)O_(2).The onset potential is as low as 0.73 V *** hydrogen electrode(RHE),the H_(2)O_(2) selectivity is 84%(0 V ***),and the average electron transfer number is about ***,the H_(2)O_(2) yield measured using a flow cell is as high as 115.92 mmol·gcat.^(−1)·h^(−1) and the Faradaic efficiency is 70%at 0 V ***,which presents high potential in electrocatalytic acidic ORR to H_(2)O_(2) and organic pollutant degradation using the electron-Fenton process.

关键词： Se defected CoSe_(2) Crystal phase transition Electrocatalysis Acidic oxygen reduction Hydrogen peroxide

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“慕课”:核心理念、实践反思与文化安全

东北师大学报（哲学社会科学版） 2014年第5期 178-186页

作者：高地东北师范大学思想政治教育研究中心吉林长春130024

"慕课"作为推动教育变革的创新举措,秉持实现优质教育资源全球共享、现代教育与信息技术深度融合的核心理念,在实践探索中面临对传统教育模式的承继与创新、多方运营成本与可持续发展、如何对待传统教育体制等深层次问题。同时,"慕课"... 详细信息

"慕课"作为推动教育变革的创新举措,秉持实现优质教育资源全球共享、现代教育与信息技术深度融合的核心理念,在实践探索中面临对传统教育模式的承继与创新、多方运营成本与可持续发展、如何对待传统教育体制等深层次问题。同时,"慕课"的兴起在文化安全上也存在对主流政治文化主导性、民族文化多样性、国民教育体系独立性的诸多现实挑战,应予以冷静审视,积极应对。

关键词：慕课核心理念实践反思文化安全

distributions and accumulation mechanisms of helium in petroliferous basins

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Science China Earth Sciences 2024年第10期67卷 3143-3168页

作者： Pengpeng LI Quanyou LIU Dongya ZHU di ZHU Zheng ZHOU Xiaoqi WU Qingqiang MENG Jiahao LV Yu gao Institute of Energy Peking UniversityBeijing 100871China Northwest Institute of Eco-Environment and Resources Chinese Academy of SciencesLanzhou 730000China Petroleum Exploration&Production Research Institute SINOPECBeijing 102206China Energy Research Institute Qilu University of Technology(Shandong Academy of Sciences)Jinan 250014China Lancaster Environment Centre Lancaster UniversityLA14YQUK Institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China

Helium is an irreplaceable strategic mineral resource, and commercial helium-rich gas fields(He>0.1%) worldwide are typically discovered serendipitously during hydrocarbon exploration efforts. According to an analysis of 75 helium-rich gas fields and 1048 natural gas samples worldwide, helium in natural gas generally exhibits “scarce”, “accompanying”, and“complex” properties, and helium-rich gas fields often occur at depths 1%). However, geological reserves in the former two types of gas fields are mainly in the range of 10^(7)–10^(11)m^(3), whereas in the latter, they are only in the range of 10^(5)–10^(7)m^(3). There are nevertheless notable disparities in the genesis and migration patterns between helium and gaseous hydrocarbons. Helium necessitates carriers(such as formation water, hydrocarbon fluids, N_(2), mantle-derived fluids, etc.) during both accumulation and long-distance migration processes, where migration conduits are not confined to sedimentary strata, and may extend to the basin's basement, lower crust, and even lithospheric mantle. However, the accumulation conditions of both helium and gaseous hydrocarbons are generally considered equivalent. The presence of gaseous hydrocarbons facilitates both the rapid exsolution of helium within helium-containing fluids and subsequent efficient aggregation in gaseous hydrocarbons, while both reduce helium diffusion and diminish escape flux. In terms of caprock, gypsum, salt, and thick shale as sealing layers contribute to the long-term preservation of helium over geological timescales. Large helium-rich gas fields, predominantly crust-derived gas fields, are primarily concentrated in uplifted zones of ancient cratonic basins and their peripheries. Based on a diagram of the He concentration versus He/N_(2) ratio, crust-derived helium fields can be categorized as basement, combined basement-s

关键词： Helium resource Geochemical characteristics Helium source rock Helium supply pattern Accumulation mechanism Determination of favorable zones

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中国共产党社会主义核心价值观理论的探索与发展

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东北师大学报（哲学社会科学版） 2013年第6期 6-11页

作者：高地东北师范大学思想政治教育研究中心吉林长春130024

科学凝练社会主义核心价值观必须坚持以党的社会主义核心价值观理论为指导,准确把握以人为本、为人民服务的根本价值取向,消灭剥削,消除两极分化,达到共同富裕的现实价值追求,实现人的自由全面发展的最高价值理想,以及以中国特色社会主... 详细信息

科学凝练社会主义核心价值观必须坚持以党的社会主义核心价值观理论为指导,准确把握以人为本、为人民服务的根本价值取向,消灭剥削,消除两极分化,达到共同富裕的现实价值追求,实现人的自由全面发展的最高价值理想,以及以中国特色社会主义核心价值体系为主体的基本价值规范。

关键词：中国共产党社会主义核心价值观理论

《〈黑格尔法哲学批判〉导言》国际比较论述厘析

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东北师大学报（哲学社会科学版） 2022年第6期 35-41页

作者：高地东北师范大学思想政治教育研究中心吉林长春130024

马克思在《〈黑格尔法哲学批判〉导言》中立足国际视野,将当时德国的理论和现实与法国、英国等国进行多重比较,探究德国解放与人类解放的重大问题,进而做出德国政治现实“时代错乱”、德国是现代各国的“理论良心”、革命理论同革命实... 详细信息

马克思在《〈黑格尔法哲学批判〉导言》中立足国际视野,将当时德国的理论和现实与法国、英国等国进行多重比较,探究德国解放与人类解放的重大问题,进而做出德国政治现实“时代错乱”、德国是现代各国的“理论良心”、革命理论同革命实践相统一、无产阶级是人类解放的主体力量等科学论断。《导言》中的国际比较论述内容丰富,内涵深刻,视野宽广,展现出青年马克思乃至早期科学社会主义理论宏阔的国际视野与战略眼光,富有现实指导意义。

关键词：《〈黑格尔法哲学批判〉导言》国际比较马克思

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立德树人:文化基因、世界经验与中国道路

东北师大学报（哲学社会科学版） 2018年第1期 26-34页

作者：高地东北师范大学思想政治教育研究中心吉林长春130024

全面落实立德树人根本任务,既要探求和解码立德树人的文化基因,也要拓开全球视野,汲取他者经验,在坚持扎根中国与融通中外的结合过程中自觉建构立德树人的中国道路。立德树人的中华文化基因主要体现为世俗性、超越性与政治性。总的来看... 详细信息

全面落实立德树人根本任务,既要探求和解码立德树人的文化基因,也要拓开全球视野,汲取他者经验,在坚持扎根中国与融通中外的结合过程中自觉建构立德树人的中国道路。立德树人的中华文化基因主要体现为世俗性、超越性与政治性。总的来看,坚持国家主导开展顶层设计、遵循青少年儿童身心发展规律进行学校德育、在全社会培育良好的道德文化氛围是国际上立德树人的主要经验。走好立德树人的中国道路,关键在于坚持以社会主义核心价值观为引领,走特色之路;扎根中国与融通中外相结合,走开放之路;不断增强德育工作的亲和力和针对性,走创新之路。

关键词：立德树人文化基因世界经验中国道路