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Chinese Physics C 2024年第5期48卷 60-71页

作者： dhananjay singh Arvind Kumar Department of Physics Dr.B R Ambedkar National Institute of Technology JalandharJalandhar–144008PunjabIndia

In the present study,we applied Tsallis non-extensive statistics to investigate the thermodynamic properties and phase diagram of quark matter in the Polyakov chiral SU(3)quark mean field *** this model,the properties of the quark matter were modified through the scalar fieldsσ,ζ,δ,χ,vector fieldsω,ρ,ϕ,and Polyakov fieldsΦandΦ¯at finite temperature and chemical ***-extensive effects were introduced through a dimensionless parameter q,and the results were compared to those of the extensive case(q→1).In the non-extensive case,the exponential in the Fermi-Dirac(FD)function was modified to a q-exponential *** influence of the q parameter on the thermodynamic properties,pressure,energy,and entropy density,as well as trace anomaly,was *** speed of sound and specific heat with non-extensive effects were also ***,the effect of non-extensivity on the deconfinement phase transition as well as the chiral phase transition of u,d,and s quarks was *** found that the critical end point(CEP),which defines the point in the(T−μ)phase diagram where the order of the phase transition changes,shifts to a lower value of temperature,TCEP,and a higher value of chemical potential,μCEP,as the non-extensivity is increased,that is,q>1.

关键词： quark matter quark mean field model QCD phase diagram non-extensive statistics

An insight into the diagnosis and pathogenesis of hepatitis C virus infection

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World Journal of Gastroenterology 2013年第44期19卷 7896-7909页

作者： Mohammad Irshad dhananjay singh mankotia Khushboo Irshad Clinical Biochemistry Division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi-110029 India Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi-110029India Department of Biochemistry All India Institute of Medical Sciences New Delhi-110029 India

This review focuses on research findings in the area of diagnosis and pathogenesis of hepatitis C virus(HCV)infection over the last few *** information based on published literature provides an update on these two aspects of *** infection,previously called blood transmitted non-A,non-B infection,is prevalent globally and poses a serious public health problem *** diagnosis of HCV infection has evolved from serodetection of non-specific and low avidity anti-HCV antibodies to detection of viral nucleic acid in serum using the polymerase chain reaction(PCR)*** PCR assays detect viral nucleic acid with high accuracy and the exact copy number of viral ***,multiplex assays using real-time PCR are available for identification of HCV-genotypes and their *** contrast to previous methods,the newly developed assays are not only fast and eco-nomic,but also resolve the problem of the window period as well as differentiate present from past *** is a non-cytopathic virus,thus,its pathogenesis is regulated by host immunity and metabolic changes including oxidative stress,insulin resistance and hepatic *** innate and adaptive immunity play an important role in HCV *** lymphocytes demonstrate crucial activity during viral eradication or viral persistence and are influenced by viral proteins,HCV-quasispecies and several metabolic factors regulating liver *** pathogenesis is a very complex phenomenon and requires further study to determine the other factors involved.

关键词： Hepatitis C virus Diagnosis Pathogenesis Immunity Steatosis

Expression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera

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Advances in Infectious Diseases 2013年第3期3卷 223-229页

作者： Shiwani singh Akanksha singh dhananjay singh mankotia Kalpana Luthra Mohammad Irshad Clinical Biochemistry Division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi India

The present study describes the cloning and expression of ORF-2 region of TTV genome and the use of expressed peptide in developing immunoassay for detection of anti-TTV antibodies in serum. Presence of TTV-DNA in serum was detected by PCR amplifying N-22 region of ORF-1 of TTV genome. This was followed by genotyping of TTV by RFLP using N-22 amplicon. Using genotype-1 positive serum as the source of TTV, the ORF-2 region was amplified by PCR and subsequently cloned and expressed in pET-19b vector. The expressed protein, identified as 20 kDa protein on SDS-PAGE gel, was purified by affinity chromatography and then used as antigen to develop western blot assay for detection of anti-TTV antibodies in serum. Analysis of sera for anti-TTV antibodies and their comparison with presence of TTV-DNA, produced encouraging results. There was a good relation between presence of anti-TTV and TTV-DNA in these sera samples. Anti-TTV antibodies could be detected in all TTV-DNA positive sera irrespective of the presence of TTV-genotype. This investigation demonstrates that ORF-2 peptide may be used in developing immunoassay for identification of TTV infection.

关键词： TTV ORF-2 Anti TTV-IgG Expression N-22

Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review

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World Journal of Gastroenterology 2016年第20期22卷 4824-4834页

作者： Mohammad Irshad Priyanka Gupta dhananjay singh mankotia Mohammad Ahmad Ansari Clinical biochemistry division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi 110029 India

The present review describes the current status of multiplex quantitative real time polymerase chain reaction(q PCR) assays developed and used globally for detection and subtyping of hepatitis viruses in body fluids. Several studies have reported the use of multiplex q PCR for the detection of hepatitis viruses, including hepatitis A virus(HAV), hepatitis B virus(HBV), hepatitis C virus(HCV), hepatitis D virus(HDV), and hepatitis E virus(HEV). In addition, multiplex q PCR has also been developed for genotyping HBV, HCV, and HEV subtypes. Although a single step multiplex q PCR assay for all six hepatitis viruses, i.e., A to G viruses, is not yet reported, it may be available in the near future as the technologies continue to advance. All studies use a conserved region of the viral genome as the basis of amplification and hydrolysis probes as the preferred chemistries for improved detection. Based on a standard plot prepared using varying concentrations of template and the observed threshold cycle value, it is possible to determine the linear dynamic range and to calculate an exact copy number of virus in the specimen. Advantages of multiplex q PCR assay over singleplex or other molecular techniques in samples from patients with co-infection include fast results, low cost, and a single step investigation process.

关键词： Co-infection Viral genome Quantitative real-time polymerase chain reaction Genotyping techniques Ser

Random Amplification Polymorphic DNA and Agro-Morphological Traits-Based Fingerprinting for Detection of Genetic Divergence in Indian Black Rice

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Rice science 2024年第4期31卷 366-370,I0004-I0014页

作者： Manisha SHARMA dhananjay RATURI Vijay Rani RAJPAL Manju CHAUDHARY Soom Nath RAINA Apekshita singh Amity Institute of Biotechnology Amity University Uttar PradeshSector-125UP 201313India Department of Botany Hansraj CollegeUniversity of DelhiDelhi 110007India

In this study, 32 black rice genotypes spanning diverse geographies in India were evaluated at both phenotypic and DNA sequence levels to gain insights into their genetic makeup. Significant variations were observed f... 详细信息

关键词： Indian Morphological Traits

Sarve:synthetic data and local differential privacy for private frequency estimation

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Cybersecurity 2022年第4期5卷 97-116页

作者： Gatha Varmal Ritu Chauhan dhananjay singh Amity Institute of Information Technology Amity UniversityNoidaIndia Center for Computational Biology and Bioinformatics Amity UniversityNoidaIndia Department of Electronics Engineering HUFSSeoulKorea

The collection of user attributes by service providers is a double-edged *** are instrumental in driving statistical analysis to train more accurate predictive models like *** analysis of the collected user data includes frequency estimation for categorical ***,the users deserve privacy guarantees against inadvertent identity *** algorithms called frequency oracles were developed to randomize or perturb user attributes and estimate the frequencies of their *** propose Sarve,a frequency oracle that used Randomized Aggregatable Privacy-Preserving Ordinal Response(RAPPOR)and Hadamard Response(HR)for randomization in combination with fake *** design of a service-oriented architecture must consider two types of complexities,namely computational and *** functions of such systems aim to minimize the two complexities and therefore,the choice of privacy-enhancing methods must be a calculated *** variant of RAPPOR we had used was realized through bloom flters.A bloom filter is a memory-efficient data structure that offers time complexity of O(1).On the other hand,HR has been proven to give the best communication costs of the order of log(b)for b-bits ***,Sarve is a step towards frequency oracles that exhibit how privacy provisions of existing methods can be combined with those of fake data to achieve statistical results comparable to the original *** also implemented an adaptive solution enhanced from the work of Arcolezi et *** use of RAPPOR was found to provide better privacy-utility tradeoffs for specific privacy budgets in both high and general privacyregimes.

关键词： Synthetic data Differential privacy Frequency estimation Frequency oracle Privacy

Assessment of Road Dust Contamination in India

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Atmospheric and Climate Sciences 2016年第1期6卷 77-88页

作者： dhananjay Sahu Shobhana Ramteke Nohar singh Dahariya Bharat Lal Sahu Khageshwar singh Patel Laurent Matini Jose Nicolas Eduardo Yubero Jan Hoinkis JK Laxmi Cement Ahiwara Durg CG India School of Studies in Chemistry/Environmental Science Pt. Ravishankar Shukla University Raipur India Department of Exact Sciences E.N.S. Marien Ngouabi University Brazzaville Congo Atmospheric Pollution Laboratory Applied Physics Department Miguel Hernandez University Elche Spain Karlsruhe University of Applied Sciences Karlsruhe Germany

The road dusts (RD) are fugitive in nature causing potential health hazards to people living in highways. They are generated from different sources on the roads and being a valuable archive of environmental information. In the present work, contamination assessment of 18 heavy metals and ions in road dusts of the country are described. Techniques i.e. ion selective, ion chromatography and atomic absorption spectrophotometers were used for analysis of the ions and metals. The content of F-, Cl-, , , , Na+, K+, Mg2+, Ca2+, As, Cr, Mn, Fe, Ni, Cu, Zn, Pb and Hg in the road dusts was ranged from 75 - 895, 276 - 12718, 48 - 1423, 243 - 10,580, 11 - 539, 290 - 46,484, 110 - 7716, 84 - 1771, 595 - 15,955, 24 - 42, 164 - 526, 1711 - 5218, 63,850 - 144,835, 47 - 62, 81 - 720, 166 - 450, 92 - 295 and 0.05 - 0.12 mg/kg with mean value of 224 ± 43, 3734 ± 895, 592 ± 895, 2859 ± 662, 143 ± 29, 4826 ± 2049, 1565 ± 411, 837 ± 121, 8545 ± 1288, 31 ± 4, 246 ± 82, 3002 ± 851, 91,331 ± 18,587, 54 ± 4, 206 ± 145, 241 ± 64, 171 ± 42 and 0.08 ± 0.02 mg/kg, respectively (at 95% probability). The enrichment, variation, correlation and sources of the contaminants are discussed.

关键词： Ions Heavy Metals Sources Road Dust

Transport Pollution in India

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American Journal of Analytical Chemistry 2015年第9期6卷 757-766页

作者： Khageshwar singh Patel dhananjay Sahu Shobhana Ramteke Borislav Blazhev Laurent Matini Eduardo Yubero Jan Hoinkis School of Studies in Environmental Science Pt. Ravishankar Shukla University Raipur India Central Laboratory for Chemical Testing and Control Sofia Bulgaria Department of Exact Sciences E.N.S. Marien Ngouabi University Brazzaville Congo Applied Physics Department Miguel Hernandez University Elche Spain Karlsruhe University of Applied Sciences Karlsruhe Germany

Road dusts contribute a large fraction of air pollution in urban environment of India. In the present work, contamination assessment of ions and elements i.e. F﹣, Cl﹣, NO-3, SO2-4, NH+4, Na+, K+, Mg2+, Ca2+, As, Cr, Mn, Fe, Ni, Cu, Zn, Pb and Hg in the road dusts of the most industrialized area of central India: Raipur (capital, Chhattisgarh state) is described during year: 2008-2013. In year 2008, the mean content of the element i.e. F﹣, Cl﹣, NO-3, SO2-4, NH+4, Na+, K+, Mg2+, Ca2+, As, Cr, Mn, Fe, Ni, Cu, Zn, Pb and Hgin the dust (n = 5) was found to be 292 ± 112, 5068 ± 2445, 927 ± 280, 3336 ± 1315, 155 ± 65, 4273 ± 1761, 1477 ± 626, 974 ± 243, 9809 ± 2370, 21.2 ± 2.4, 150 ± 30, 12,816 ± 12,522, 157,736 ± 61,542, 60 ± 7, 566 ± 608, 348 ± 154, 296 ± 163 and 0.10 ± 0.09 mg/kg, respectively. The enrichment, concentration variations and sources of the elements are discussed.

关键词： Road Dust Heavy Metals Ions Contamination India

Contamination of Water, Dust, Soil, Rock and Urine with Fluoride in Central India

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Journal of Environmental Protection 2015年第12期6卷 1347-1359页

作者： Khageshwar singh Patel dhananjay Sahu Nohar singh Dahariya Bharat Lal Sahu Shobhana Ramteke Borislav Blazhev Laurent Matini Eduardo Yubero Jan Hoinkis School of Studies in Chemistry/Environmental Science Pt. Ravishankar Shukla University Raipur India Central Laboratory for Chemical Testing and Control Sofia Bulgaria Department of Exact Sciences Marien Ngouabi University Brazzaville Congo Applied Physics Department Miguel Hernandez University Elche Spain Karlsruhe University of Applied Sciences Karlsruhe Germany

At least 15% of 0.1 million people residing in 117 villages of Tamnar block (Chhattisgarh, central India) are suffering from fluorosis diseases. In this work, the contamination of F- in the environment (i.e. water, soil, rock and urine) of the Tamnar block is described. The concentration variations of F-, Cl-, NO3-, SO42-, Na+, K+, Mg2+, Ca2+, Al, Mn, Fe and Zn in the groundwater are reported. The F- content in the water was ranged from 1.7 - 17 mg/L with mean value of 9.0 ± 3.7 mg/L. Fluoride was enriched up to 3-, 54-, 69- and 244-folds in the urine, soil, dust and rock, respectively. The cluster and factor analysis models were used to apportion sources of F- and other elements in the water.

关键词： Fluoride Contamination Groundwater Soil Rock India

Tobacco Exposed Buccal Mucosa Graft—A Potential Carcinogen for Scrotal Cancer

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Surgical Science 2012年第2期3卷 87-88页

作者： Chaitanya H. Raut Atul Kumar Fanindra singh Solanki Shabbir Hussain Deepti B. Sharma dhananjay Sharma Department of Surgery NSCB Medical College Jabalpur India

Squamous cell carcinoma of scrotum is not common. It was the first cancer directly associated with a specific occupation i.e. chimney sweeps. We report a case of squamous cell carcinoma of scrotum developed in a patient of stricture urethra with multiple perineal urinary fistulas treated with lay open urethra with buccal mucosal graft. Tobacco exposed buccal mucosa graft can act as a carcinogen for scrotal cancer in patients with multiple fistula and poor hygiene.

关键词： Buccal Mucosa Scrotal Malignancy Tobacco