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Chinese Chemical Letters 2024年第8期35卷 304-308页

作者： Zhili Li Qijun Wo Dongdong Huang dezhong zhou Lei Guo Yeqing Mao Department of Urology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310000China School of Chemical Engineering and Technology Xi’an Jiaotong UniversityXi’an 710049China Urology&Nephrology Center Department of UrologyZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou 310014China Key Laboratory of Endocrine Gland Diseases of Zhejiang Province Hangzhou 310014China Pooling Institute of Translational Medicine Hangzhou 311100China

Highly branched poly(β-amino ester)s(HPAEs)have emerged as a safe and efficient type of non-viral gene delivery ***,the presence of inactive terminal secondary amine groups compromises their gene transfection *** this study,HPAEs with similar topological structures and chemical compositions but varying numbers of terminal secondary 4-amino-1-butanol(S4)and secondary/tertiary 3-morpholinopropylamine(MPA)groups were *** results demonstrate that an increased number of secondary/tertiary MPA groups in-situ significantly enhances the DNA binding capability of HPAEs,leading to the formation of smaller HPAE/DNA polyplexes with higher zeta potential,ultimately resulting in superior gene transfection efficiency in bladder epithelial *** study establishes a sim-ple yet effective strategy to maximize the gene transfection potency of HPAEs by converting the inactive terminal groups in-situ without the need for complex modifications to their topological structure and chemical composition.

关键词： Gene therapy Gene delivery vector Highly branched poly(β-amino ester)s Terminal groups Epithelial cells

Highly branched poly(β-amino ester)s with narrow molecular weight distribution: Fractionation and gene transfection activity

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Chinese Chemical Letters 2023年第3期34卷 383-387页

作者： Chenfei Wang Litao Sun Qiuxia Li Zhili Li Chengyuan Xu Xinyue Zhang Jianjun Shi Hao zhou Wenxin Wang dezhong zhou School of Chemical Engineering and Technology Xi’an Jiaotong UniversityXi’an 710049China Key Laboratory of Water Pollution Treatment&Resource Reuse Hainan Normal UniversityHaikou 571158China State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Protein Scienceand College of Life SciencesNankai UniversityTianjin 300071China Department of Dermatology The Second Hospital Affiliated to Xi’an Jiaotong UniversityXi’an 710061China Charles Institute of Dermatology School of MedicineUniversity College DublinDublin 4Ireland

Highly branched poly(β-amino ester)s(HPAEs) have shown their great promise in gene delivery. However, their broad molecular weight distribution(MWD) poses an additional challenge to the mechanistic understanding of the influence of molecular weight(MW) on their gene transfection activity. Using a stepwise precipitation strategy, HPAEs were fractionated. It is shown that MW has a significant effect on the transfection activity and cytotoxicity of HPAEs. The intermediate MW mediates higher transfection efficiency while maintaining high cell viability. Mechanistic studies show that the intermediate MW confers stronger DNA binding affinity to HPAEs, leading to the formulation of polyplexes with a relatively smaller size and more positive zeta potential. This study not only suggests a simple strategy to fractionate HPAEs with narrow MWD but also provides new insights into understanding the structure-property relationship, which would facilitate the clinical translation of HPAEs in gene therapy.

关键词： Gene transfection Non-viral vector Highly branched poly(β-amino ester)s Fractionation Transfection activity

Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)

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Chinese Chemical Letters 2023年第7期34卷 176-181页

作者： Delu Che Chenfei Wang Zhili Li Kaixuan Wang Shuaiwei Sun Xinyue Zhang Yi Li Zhengju Chen Lei Guo Yajing Hou dezhong zhou Songmei Geng Department of Dermatology the Second Hospital Affiliated to Xi'an Jiaotong UniversityXi'an 710061China School of Chemical Engineering and Technology Xi'an Jiaotong UniversityXi'an 710049China Infectious Disease Department the Second Xiangya Hospital of Central South UniversityChangsha 410011China Pooling Medical Research Institutes Hangzhou 310053China Department of Pharmacy Shaanxi Provincial People's HospitalXi'an 710068China

Suspension cells play a crucial role in many biological processes. However, compared to adherent cells, it is particularly challenging to introduce exogenous genes into suspension cells to regulate their biological functions with non-viral gene vectors, mainly due to the low cellular uptake and endosomal escape of polyplexes. Herein, to improve the interactions of polyplexes with cellular membranes, we design and synthesize highly branched poly(β-amino ester)(HPAE) via an “A2 + B4 + C2” Michael addition *** show that branching significantly increases DNA condensation of HPAE, cellular uptake and endosomal escape of HPAE/DNA polyplexes. In mast cells(MCs), HPAE exhibits up to 80-fold higher gene transfection efficiency compared to the corresponding linear poly(β-amino ester)(LPAE) and the leading commercial gene transfection reagents PEI25k, jetPEI, and Lipofectamine 3000, without causing obvious cytotoxicity. Our study establishes a reliable non-viral platform for efficient gene transfection of suspension cells.

关键词： Non-viral vector Highly branched poly(β-amino ester) Gene transfection High transfection efficiency Suspension cells Mast cells

内环化聚(β-氨基酯)的制备与生物物理性能

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高等学校化学学报 2024年第2期45卷 133-138页

作者：雍海洋李智立郭蕊周德重西安交通大学化学工程与技术学院西安710049

拓扑结构对聚合物性能具有重要影响.本文通过调控聚合条件制备内环化聚(β-氨基酯)并探究其生物物理性能.以三羟甲基丙烷三丙烯酸酯(TMPTA)和4-吗啉丙胺(MPA)为单体并控制其投料比及体系单体浓度,利用迈克尔加成反应制备了两种内环化聚(... 详细信息

拓扑结构对聚合物性能具有重要影响.本文通过调控聚合条件制备内环化聚(β-氨基酯)并探究其生物物理性能.以三羟甲基丙烷三丙烯酸酯(TMPTA)和4-吗啉丙胺(MPA)为单体并控制其投料比及体系单体浓度,利用迈克尔加成反应制备了两种内环化聚(β-氨基酯).使用凝胶渗透色谱仪(GPC)、核磁共振波谱仪(NMR)和原子力显微镜(AFM)等对内环化聚(β-氨基酯)的化学组成、拓扑结构和生物物理性能进行表征与分析.结果表明,随着环化度的增加,聚(β-氨基酯)对DNA压缩能力增强,降解速率加快,质子缓冲能力提高,细胞毒性增大.本文研究结果为制备和表征内环化聚(β-氨基酯)提供了新思路,也为设计有效的基因递送载体提供了依据.

关键词：拓扑结构内环化聚(β-氨基酯) 投料比生物物理性能

Application of Generalized Cross Validation in Temperature Field Measurement

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Application of Generalized Cross Validation in Temperature F...

O_(3)-NaNi_(0.4)Fe_(0.2)Mn_(0.4)O_(2)正极Na^(+)传输动力学及相变机制

第一届国际测量仪器与测试自动化学术会议

作者： Zheng dezhong zhou Yinghui Jing Nan (Yanshan University Qinhuangdao 066004 China)

＜正＞The measurement method of flame temperature field using the matrix CCD has been developed. However, much unknown noise in flame images would prevent from obtaining temperature information with accuracy. To eliminate the noise, the analysis algorithm uses wavelet demising by soft-threshold. An important parameter for this procedure is threshold. Conventional methods for obtaining the threshold are suitable for uncorrelated noise and require to estimate the variance of noise. For a signal containing unknown noise, the paper presents using Generalized Cross Validation law to get optimal threshold （GCV-threshold）. This procedure does not require prior knowledge of noise level and is proved to be asymptotically optimal. For comparing with different thresholds, an artificial noisy image is processed by universal-threshold, SURE-threshold and GCV-threshold respectively. The results show whether b) observation or signal-to-noise ratio based on mean square error criterion as an objective quality measure, GCV procedure is superior to others. Furthermore, the method is applied in our temperature field measurement system. The results show the accuracy is improved 1.08% around.

关键词： Temperature field measurement two-color method wavelet image denoising GCV law.

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储能科学与技术 2023年第4期12卷 1011-1017页

作者：周亚男滑纬博周德重西安交通大学陕西西安710049

钠离子电池因其成本低廉、环境友好且与锂离子电池工作原理相似,在大规模储能领域极具应用潜力。作为决定电池能量密度的关键组成部分,O3型钠基层状过渡金属氧化物因高容量、合成简单等优势在众多正极材料中脱颖而出。然而,Na^(+)在O3... 详细信息

钠离子电池因其成本低廉、环境友好且与锂离子电池工作原理相似,在大规模储能领域极具应用潜力。作为决定电池能量密度的关键组成部分,O3型钠基层状过渡金属氧化物因高容量、合成简单等优势在众多正极材料中脱颖而出。然而,Na^(+)在O3结构中八面体位点间的迁移需克服较大的能垒,最终导致复杂反应相变的发生和容量快速衰减。因此,探究O3型正极材料电化学反应过程中Na^(+)脱嵌行为与结构演变的构效关系对开发高性能正极材料至关重要。本工作以O_(3)-NaNi_(0.4)Fe_(0.2)Mn_(0.4)O_(2)(O3-NFM)正极为研究对象,对其电化学性能、Na^(+)传输动力学性质及相变机制展开了系统研究。电化学测试结果表明,O3-NFM在充电至高压(4.3 V)时可脱出0.84 mol Na^(+),发挥约201.9 mAh/g的比容量,但可逆性欠佳。当截止电压为4.0 V时,该正极材料循环性能优异,原位XRD结果进一步证明了电化学反应过程中O3-P3/O3-P3-P3/O3-O3的可逆结构转变。循环伏安(CV)曲线和恒电流间歇滴定技术(GITT)结果表明其具有快速的钠离子扩散速率,从而表现出较好的倍率性能。本研究为探索以O3-NFM为基础的正极材料结构设计及性能调控提供了理论基础。

关键词：钠离子电池过渡金属氧化物 O_(3)-NaNi_(0.4)Fe_(0.2)Mn_(0.4)O_(2) 原位XRD

血清HE4、CA125和TSGF联合检测在卵巢肿瘤中的诊断价值

同方期刊数据库博看期刊评论

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航空航天医学杂志 2015年第7期26卷 798-799页

作者：周德众抚顺矿务局总医院检验科抚顺113008

目的分析研究血清人附睾分泌蛋白4（HE4）、糖类抗原125（CA125）和恶性肿瘤特异性生长因子（TSGF）联合检测在卵巢肿瘤中的诊断价值。方法选择2014年1月~2014年6月妇产科治疗的卵巢恶性肿瘤患者73例,卵巢良性肿瘤患者73例,同时选取7... 详细信息

目的分析研究血清人附睾分泌蛋白4（HE4）、糖类抗原125（CA125）和恶性肿瘤特异性生长因子（TSGF）联合检测在卵巢肿瘤中的诊断价值。方法选择2014年1月~2014年6月妇产科治疗的卵巢恶性肿瘤患者73例,卵巢良性肿瘤患者73例,同时选取70例健康体检女性。采用酶联免疫分析（ELISA）法测定血清HE4水平,采用电化学发光法（ECLIA）检测血清CA125水平,采用比浊法检测TSGF水平。结果血清HE4、CA125和TSGF的表达水平在卵巢恶性肿瘤组明显高于其他两组,组间比较差异有统计学意义（P〈0.05）;良性肿瘤组和健康组血清HE4、CA125和TSGF水平比较差异有统计学意义（P〈0.05）;血清HE4、CA125和TSGF各单项及联合检测诊断卵巢肿瘤的阳性率与健康对照组比较差异有统计学意义（P〈0.05）;三项指标联合检测卵巢肿瘤的阳性率显著高于各指标的单项检测（P〈0.05）。结论血清HE4、CA125和TSGF联合检测有较高的灵敏度,可以明显提高卵巢肿瘤诊断的准确性,在卵巢肿瘤的诊断中具有重要的临床意义。

关键词：卵巢肿瘤人附睾分泌蛋白4 糖类抗原125 恶性肿瘤特异性生长因子

菊粉对断奶仔猪生长性能和免疫功能的作用研究

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饲料工业 2011年第24期32卷 36-38页

作者：王中华周德忠商丘职业技术学院

试验旨在探讨菊粉对断奶仔猪生长性能和免疫功能的作用,采用单因子随机分组设计,选取30日龄三元杂交(杜×长×大)仔猪64头,随机分成4组(对照组和试验1、2、3组),每组4个重复,分别饲喂含0%、1%、1.5%、2%菊粉的日粮,试验为期5周。结果表... 详细信息

试验旨在探讨菊粉对断奶仔猪生长性能和免疫功能的作用,采用单因子随机分组设计,选取30日龄三元杂交(杜×长×大)仔猪64头,随机分成4组(对照组和试验1、2、3组),每组4个重复,分别饲喂含0%、1%、1.5%、2%菊粉的日粮,试验为期5周。结果表明:与对照组相比,试验1组、2组和3组平均日增重、脾脏指数、胸腺指数和IgA水平显著提高(P<0.05),腹泻指数显著降低(P<0.05),试验1组和2组与对照组相比,日均采食量显著提高(P<0.05)。菊粉可提高断奶仔猪生产性能和免疫功能,且饲料中添加1.5%的菊粉效果最好。

关键词：菊粉断奶仔猪生长性能免疫功能

急性脑梗死患者血清chemerin检测的临床意义

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航空航天医学杂志 2016年第1期27卷 29-31页

作者：周德众抚顺矿务局总医院检验科抚顺113008

目的为了解血清Chemerin检测在急性脑梗死患者中的临床意义。方法清晨空腹采静脉血,采用酶联免疫吸附试验测定Chemerin水平;采用散射比浊法测定C反应蛋白;采用全自动生化分析仪检测血糖血脂。结果与对照组相比,急性脑梗死组患者的空腹... 详细信息

目的为了解血清Chemerin检测在急性脑梗死患者中的临床意义。方法清晨空腹采静脉血,采用酶联免疫吸附试验测定Chemerin水平;采用散射比浊法测定C反应蛋白;采用全自动生化分析仪检测血糖血脂。结果与对照组相比,急性脑梗死组患者的空腹血糖、甘油三酯及低密度脂蛋白胆固醇水平均较高,且两组相比差异具有统计学意义(P<0.05),Chemerin水平、C反应蛋白水平也显著升高,两组差别显著(P<0.01)。而急性脑梗死组患者的高密度脂蛋白胆固醇显著低于对照组(P<0.05)。急性脑梗死患者的Chemerin水平与体重指数、空腹血糖、甘油三酯、低密度脂蛋白胆固醇、C反应蛋白及NIHSS神经缺损评分呈正相关,而与年龄、高密度脂蛋白胆固醇水平无相关。结论急性脑梗死患者血浆Chemerin升高的程度与观察对象的神经功能缺损程度之间存在明显的相关性,也可以说是在某些方面反映了病变的现状通过监测Chemerin的水平,为评价急性脑梗死治疗的有效性及疾病防控方面带来充足的论据。随着临床研究及Chemerin的作用机制被逐渐认识,Chemerin极有可能会成为临床上心血管疾病防控的新靶点。

关键词：急性脑梗死 Chemerin水平 C反应蛋白胆固醇

青贮玉米籽实对断奶仔猪生长性能和免疫功能的影响

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粮食与饲料工业 2011年第10期 51-53页

作者：王中华周德忠商丘职业技术学院河南商丘476100

探讨了青贮玉米籽实对断奶仔猪生长性能和免疫功能的影响。结果表明:饲喂添加1.5%(试验1组)、2.5%(试验2组)、3.5%(试验3组)青贮玉米籽实的试验组与对照组相比腹泻指数分别降低了42.4%、54.4%、49.6%(P<0.05),试验1组、试验2组与对照组... 详细信息

探讨了青贮玉米籽实对断奶仔猪生长性能和免疫功能的影响。结果表明:饲喂添加1.5%(试验1组)、2.5%(试验2组)、3.5%(试验3组)青贮玉米籽实的试验组与对照组相比腹泻指数分别降低了42.4%、54.4%、49.6%(P<0.05),试验1组、试验2组与对照组相比日均采食量分别提高了12.40%、11.29%(P<0.05),平均日增重分别提高了15.23%、12.53%(P<0.05),脾脏指数分别提高了25.47%和20.50%(P<0.05),胸腺指数分别提高了32.20%和26.27%(P<0.05),血清IgA水平分别提高了24.59%和18.03%(P<0.05)。综合各项指标,饲料中添加2.5%的青贮玉米籽实效果较理想。

关键词：青贮玉米籽实断奶仔猪生长性能免疫功能