Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)

作     者：Delu Che Chenfei Wang Zhili Li Kaixuan Wang Shuaiwei Sun Xinyue Zhang Yi Li Zhengju Chen Lei Guo Yajing Hou Dezhong Zhou Songmei Geng Delu Che;Chenfei Wang;Zhili Li;Kaixuan Wang;Shuaiwei Sun;Xinyue Zhang;Yi Li;Zhengju Chen;Lei Guo;Yajing Hou;Dezhong Zhou;Songmei Geng

作者机构：Department of Dermatologythe Second Hospital Affiliated to Xi'an Jiaotong UniversityXi'an 710061China School of Chemical Engineering and TechnologyXi'an Jiaotong UniversityXi'an 710049China Infectious Disease Departmentthe Second Xiangya Hospital of Central South UniversityChangsha 410011China Pooling Medical Research InstitutesHangzhou 310053China Department of PharmacyShaanxi Provincial People's HospitalXi'an 710068China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2023年第34卷第7期

页      面：176-181页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 0703[理学-化学] 10[医学] 

基　　金：funded by National Natural Science Foundation of China (NSFC, No. 51903202) the Innovation Capability Support Program of Shaanxi (No. 2022TD-48) the Key R&D Program of Shaanxi Province (No. 2020GXLH-Y-016) 

主　　题：Non-viral vector Highly branched poly(β-amino ester) Gene transfection High transfection efficiency Suspension cells Mast cells 

摘      要：Suspension cells play a crucial role in many biological processes. However, compared to adherent cells, it is particularly challenging to introduce exogenous genes into suspension cells to regulate their biological functions with non-viral gene vectors, mainly due to the low cellular uptake and endosomal escape of polyplexes. Herein, to improve the interactions of polyplexes with cellular membranes, we design and synthesize highly branched poly(β-amino ester)(HPAE) via an “A2 + B4 + C2 Michael addition *** show that branching significantly increases DNA condensation of HPAE, cellular uptake and endosomal escape of HPAE/DNA polyplexes. In mast cells(MCs), HPAE exhibits up to 80-fold higher gene transfection efficiency compared to the corresponding linear poly(β-amino ester)(LPAE) and the leading commercial gene transfection reagents PEI25k, jetPEI, and Lipofectamine 3000, without causing obvious cytotoxicity. Our study establishes a reliable non-viral platform for efficient gene transfection of suspension cells.