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Rapid Access to Chiralα-Alkylated Azacycles via NiH-Catalyzed Asymmetric Migratory Hydroalkylation of N-Heterocyclic Alkenes

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CCS Chemistry 2024年第4期6卷 898-904页

作者： Changpeng Chen Wenqing Guo deyong Qiao Junqian Zhou you Wang Shaolin Zhu State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic MaterialsChemistry and Biomedicine Innovation Center(ChemBIC)School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210093 School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007 Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong UniversityShanghai 200240

Asymmetric migratory hydrofunctionalization of isomeric mixtures of alkenes provides a complementary approach for the selective introduction of a functional group into a remote C(sp3)-H ***,the simultaneous control of both regio-and enantioselectivity in this migratory process is a ***α-alkyl-substituted azacycles are key motifs in pharmaceuticals,natural products,and ***,we report a NiH-catalyzed asymmetric migratory hydroalkylation process for their efficient and selective *** this process,easily available isomeric mixtures of N-heterocyclic alkenes can be directly employed as starting materials,convergently delivering a variety of enantioenrichedα-alkylated azacycles with high levels of regio-and *** synthetic utility of this transformation is demonstrated by the facile synthesis of two bioactive alkaloids,(+)-connine and(R)-*** obtained chiralα-alkylated azacycles also readily undergo diverse derivatization,such as deconstructive halogenation and ring contraction.

关键词： alkenes alkylation asymmetric catalysis isomerization nickel

Molecular Mechanism of Rice Necrotic Lesion for Optimized Yield and Disease Resistance

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Rice science 2024年第3期31卷 285-299页

作者： HOU Xinyue WANG Yuping QIAN Qian REN deyong State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Rice Research InstituteSichuan Agricultural UniversityChengdu 611130China

How to balance rice resistance and yield is an important issue in rice *** with mutated necrotic lesion genes often have persistent broad-spectrum resistance,but this broad-spectrum resistance usually comes at the expense of ***,many necrotic lesion mutants in rice have been identified,and these genes are involved in disease resistance *** review provides a detailed introduction to the characteristics,classification,and molecular mechanisms of necrotic lesion ***,we review the molecular regulatory pathways of genes involved in rice disease ***,we summarize the relationship between resistance and yield in rice using newly developed gene editing *** discuss a rational and precise breeding strategy to better utilize molecular design technology for breeding disease-resistant and high-yield rice varieties.

关键词： rice necrotic lesion regulation mechanism high yield high resistance

Optimization of Center of Gravity Position and Anti-Wave Plate Angle of Amphibious Unmanned Vehicle Based on Orthogonal Experimental Method

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Computer Modeling in Engineering & Sciences 2024年第5期139卷 2027-2041页

作者： deyong Shang Xi Zhang Fengqi Liang Chunde Zhai Hang Yang Yanqi Niu School of Mechanical Electronic&Information EngineeringChina University of Mining and Technology(Beijing)Beijing100083China Institute of Intelligent Mining&Robotics China University of Mining and Technology(Beijing)Beijing100083China

When the amphibious vehicle navigates in water,the angle of the anti-wave plate and the position of the center of gravity greatly influence the navigation *** the relevant research on reducing the navigation resistance of amphibious vehicles by adjusting the angle of the anti-wave plate,there is a lack of scientific selection of parameters and reasonable research of simulation results by using mathematical methods,and the influence of the center of gravity position on navigation characteristics is not considered at the same *** study the influence of the combinations of the angle of the anti-wave plate and the position of the center of gravity on the resistance reduction characteristics,a numerical calculation model of the amphibious unmanned vehicle was established by using the theory of computational fluid dynamics,and the experimental data verified the correctness of the numerical *** on this numerical model,the navigation characteristics of the amphibious unmanned vehicle were studied when the center of gravity was located at different positions,and the orthogonal experimental design method was used to optimize the parameters of the angle of the anti-wave plate and the position of the center of *** results show that through the parameter optimization analysis based on the orthogonal experimental method,the combination of the optimal angle of the anti-wave plate and the position of the center of gravity is *** the numerical simulation result of resistance is consistent with the predicted optimal *** with the maximum navigational resistance,the parameter optimization reduces the navigational resistance of the amphibious unmanned vehicle by 24%.

关键词： Amphibious unmanned vehicle orthogonal experimental design anti-wave plate center of gravity resistance characteristic

Rice AGL1 determines grain size and sterile lemma identity

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The Crop Journal 2024年第2期12卷 630-634页

作者： Haiping Yu An Wang Guangheng Zhang Guojun Dong Longbiao Guo Qian Qian deyong Ren State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006ZhejiangChina Hainan Yazhou Bay Seed Laboratory Sanya 572024HainanChina National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024HainanChina

The grass spikelet is a unique inflorescence structure that determines grain *** many genetic factors have been well characterized for grain size and glume development,the underlying molecular mechanisms in rice are far from ***,we isolated rice gene,AGL1 that controlled grain size and determines the fate of the sterile *** of function of AGL1 produced larger grains and reduced the size of the sterile *** grains in the agl1 mutant were caused by a larger number of cells that were longer and wider than in the wild *** sterile lemma in the mutant spikelet was converted to a rudimentary glume-like *** findings showed that the AGL1(also named LAX1)protein positively regulated G1 expression,and negatively regulated NSG1 expression,thereby affecting the fate of the sterile *** together,our results revealed that AGL1 played a key role in negative regulation of grain size by controlling cell proliferation and expansion,and supported the opinion that rudimentary glume and sterile lemma in rice are homologous organs.

关键词： Abnormal grain and sterile lemma 1 Oryza sativa Grain size Rudimentary glume

A resonant high-pressure microsensor based on a composite pressure-sensitive mechanism of diaphragm bending and volume compression

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Microsystems & Nanoengineering 2024年第2期10卷 57-66页

作者： Pan Qian Zongze Yu Jie Yu Yulan Lu Bo Xie Jian Chen deyong Chen Junbo Wang The State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing100190China The School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing100049China

In this paper,a composite pressure-sensitive mechanism combining diaphragm bending and volume compression was developed for resonant pressure microsensors to achieve high-pressure measurements with excellent *** composite mechanism was explained,and the sensor structure was designed based on theoretical analysis and finite element *** all-silicon resonant high-pressure microsensor with multiple miniaturized cavities and dual resonators was developed,where dual resonators positioned in two resonant cavities with suitably different widths are used to perform opposite characteristics in pressure and the same characteristics at different temperatures,which can improve pressure sensitivities and realize temperature self-compensation by differential frequency *** microsensor was fabricated by microfabrication,and the experimental results showed that the sensor had an accuracy of±0.015%full scale(FS)in a pressure range of 0.1~100 MPa and a temperature range of−10~50℃.The pressure sensitivity of the differential frequency was 261.10 Hz/MPa(~2523 ppm/MPa)at a temperature of 20℃,and the temperature sensitivities of the dual resonators were−1.54 Hz/℃(~−14.5 ppm/℃)and−1.57 Hz/℃(~−15.6 ppm/℃)at a pressure of 2 *** differential output had an outstanding stability within±0.02 Hz under constant temperature and ***,this research provides a convenient solution for high-pressure measurements because of its advantages,namely,large range,excellent accuracy and stability.

关键词： stability resonant bending

Smad8 is involvement in follicular development via the regulation of granulosa cell growth and steroidogenesis in mice

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BIOCELL 2024年第1期48卷 139-147页

作者： DAOLUN YU deyong SHE KAI GE LEI YANG RUINA ZHAN SHAN LU YAFEI CAI College of Biotechnology and Pharmaceutical Engineering West Anhui UniversityLu’an237012China Lu’an Academy of Agricultural Sciences Lu’an237001China College of Life Sciences Anhui Normal UniversityWuhu241000China College of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095China

Background:SMAD family proteins(SMADs)are crucial transcription factors downstream of transforming growth factor beta(TGF-ß)/SMAD signaling pathways that have been reported to play a pivotal role in mammalian ***,the role of SMAD family member 8(SMAD8,also known as SMAD9),a member of the SMAD family,in mammalian reproduction remains ***:We employed RNA interference techniques to knock down Smad8 expression in mouse granulosa cells(GCs)to investigate the effects of Smad8 on GC growth and ***:Our findings revealed a significant decrease in the proliferative capacity and a substantial increase in the apoptosis rate of GCs after transfection with Smad8-siRNA for 48 *** hormone assays demonstrated a significant decrease in estradiol(E2)levels,whereas progesterone(P4)remained *** mechanistic analysis showed that the mRNA expression of proliferating cell nuclear antigen(Pcna),Cyclin D2,cell cycle-dependent kinase 4(Cdk4),B-cell lymphoma-2(Bcl-2),estrogen receptor(Er),luteinizing hormone receptor(Lhr)and cytochrome P450 family 19 subfamily A member 1(Cyp19a1)significantly ***,the mRNA of cysteine aspartate proteinase 3(Caspase 3)significantly increased,wheras Bcl2-associated X(Bax),folliclestimulating hormone receptor(Fshr)and cytochrome P450 family 11 subfamily A member 1(Cyp11a1)remained unchanged compared to the ***:This study indicates that Smad8 knockdown inhibits cell proliferation,promotes apoptosis,reduces Er and Lhr transcription,and decreases E2 production in mouse *** findings suggest that Smad8 may serve as a novel genetic marker for mammalian reproduction.

关键词： Mouse Smad8 RNA interference Granulosa cells Growth and steroidogenesis

The Novel Long QT Syndrome Type 2-associated F129I Mutation in the KCNH2 Gene Significantly Affects IKr Through the hERG1 Homomeric and Heteromeric Potassium Channels

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Cardiology Discovery 2024年第2期4卷 174-182页

作者： Li Feng Kejuan Ma Xin Li Nian Liu deyong Long Changsheng Ma Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityNational Clinical Research Center for Cardiovascular DiseasesBeijing 100029China Community Health Service Center of Beijing Nomal University Beijing 100875China.

Objective:The long QT syndrome type 2 is caused by the loss-of-function mutations in the KCNH2 gene,which encodes hERG1,the voltage-gated potassium *** hERG1 channels conduct rapid delayed rectifier K^(+)currents(I_(Kr))in the human cardiac ***2 encodes 2 main isoforms-hERG1a and hERG1b,which assemble to form the homomeric or heteromeric hERG1 ***,the functional characteristics of the heteromeric hERG1 channels in long QT syndrome type 2 are not *** this study,a novel mutation in the N-terminus of hERG1a(F129l)was identified in a proband of long QT syndrome type *** purpose of this study was to identify the electrophysiological change of homomeric and heteromeric hERG1 channels with ***:Candidate genes were screened by direct sequencing.F129l-hERG1a was cloned in the pcDNA3.1 vector by site-directed ***,the wild-type(WT)hERG1a and/or F129l-hERG1a were transiently expressed in the HEK293 cells with or without hERG1b *** expression levels of the transgenes,cellular distribution of hERG1a and hERG1b,and the electrophysiological features of the homomeric and the heteromeric hERG1 channels with the WT-hERG1a or F129l-hERG1a were analyzed using whole-cell patch-clamp electrophysiology,western blotting,and immunofluorescence ***:The proband was clinically diagnosed with long QT syndrome type 2 and carried a heterozygous mutation c.385T>A(F1291)in the KCNH2 *** study proved that the F129l substitution in hERG1a significantly decreased I_(Kr) in both the homomeric and heteromeric hERG1channels by 86%and 70%,respectively(WT-hERG1a(54.88±18.74)pA/pF vs.F129l-hERG1a(7.34±1.90)pA/pF,P<0.001;WT-hERG1a/hERG1b(89.92±24.51)pA/pF vs.F129l-hERG1a/hERG1b(26.54±9.83)pA/pF,P<0.001).The voltage dependence of I_(Kr) activation(V_(1/2) and k)was not affected by the mutation in both the homomeric and heteromeric hERG1 *** peak current densities and the kinetic charact

关键词： Long QT syndrome Arrhythmia lon channels hERG

A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity

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Journal of Integrative Agriculture 2024年第6期23卷 1967-1978页

作者： Lijie Chen Qianze Peng Xiaohua Du Weixing Zhang Ju’e Cheng Shu’e Sun deyong Zhang Pin Su Yong Liu Longping Branch College of BiologyHunan UniversityChangsha 410082China State Key Laboratory of Hybrid Rice/Institute of Plant Protection Hunan Academy of Agricultural SciencesChangsha 410125China

Plant virus causes massive crop losses ***,there is currently no effective measure to control plant viral ***,we identify an antiviral protein Rhp-PSP,produced by the bacterial Rhodopseudomonas palustris strain *** this study,we discover that the antiviral activity of Rhp-PSP relies on its endoribonuclease *** the arginine(R)residue at position 129 onto alanine(A)abolishs its endoribonuclease activity on coat protein(CP)RNA of tobacco mosaic virus(TMV),consequentially,compromises the antiviral activity of *** investigation demonstrates that,the mutant Rhp-PSP^(R129A)is unable to form the homotrimer as the wild type,indicating the importance of quaternary junction for the endoribonuclease *** of Rhp-PSP in Nicotiana benthamiana significantly enhances the resistance against TMV of seedlings,while expression of Rhp-PSP^(R129A)did not,confirming that endoribonuclease activity is responsible for the antiviral activity of *** addition,foliar spray of Rhp-PSP solution on tomato and pepper plants significantly reduces the disease index of viral diseases,indicating that Rhp-PSP shows potential to develop antiviral agent in practice.

关键词： Rhodopseudomonas palustris Rhp-PSP tobacco mosaic virus endoribonuclease antiviral activity

Carotenoid isomerase regulates rice tillering and grain productivity by its biosynthesis pathway

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Journal of Integrative Plant Biology 2024年第2期66卷 172-175页

作者： Chaoqing Ding Zhengji Shao Yuping Yan Guangheng Zhang Dali Zeng Li Zhu Jiang Hu Zhenyu Gao Guojun Dong Qian Qian deyong Ren State Key Laboratory of Rice Biology and Breeding ChinaNational Rice Research InstituteHangzhou 310006China

Tillers are unique inflorescence-like branches in grasses,and their number determines the panicle number,plant architecture,and yield(Shang et al.,2021).Tiller formation mainly undergoes axillary meristem(AM)initiation and tiller bud outgrowth(Wang et al.,2018;Yan et al.,2023).The rice(Oryza sativa)KNOX gene OSH1 is expressed in AMs,and an osh1 mutant produces fewer tillers(Tanaka et al.,2015).

关键词： sativa Oryza panicle