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The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth

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Science china(Life Sciences) 2004年第1期47卷 25-30页

作者： GU Zhiliang, ZHU Dahai, LI Ming, LI Hui, DENG Xuemei & WU Changxinmolecular and Cellular Developmental biology laboratory, Harbin Institute of Technology, Harbin 150001, china college of Animal Science & Technology, Northeast Agricultural University, Harbin 150030, china national Laboratories for Agribiotechnology, china Agricultural University, beijing 100094, china college of Animal Science & Technology, china Agricultural University, beijing 100094, china department of Life Science and Technology, Changshu Institute of Technology, Changshu 215500, china department of biochemistry and molecular biology, national laboratory of medical molecular biology, peking union medical college, beijing 100005, china

Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single nucleotide polymorphisms (SNPs) in the chicken my-ostatin gene. Herein, we investigate the association of those SNPs with the production traits in a F2 chicken line derived from Broilers crossing to Silky with the least square analysis. The results show that the BB and AA genotypes are strongly associated with abdominal fat weight (AFW), abdominal fat percentage (AFP), and birth weight (BW) (P < 0.05). Breast muscle percentage (BMP) of the AA type is higher than that of the AB type. The breast muscle weight and breast muscle percentages of F2 individuals have significant difference between CC and DD genotypes (P< 0.05). Breast muscle weight (BMW) of EF birds is higher than that of EE birds (P< 0.05). In this report, we present the first genetic evidence to show that chicken myostatin not only plays an important role in controlling skeletal muscle growth and differentiation, but also may be involved in regulation of adipose growth in chicken.

关键词： myostatin gene, SNPs, F2 chicken population, abdominal fat, breast muscle weight.

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In Memoriam: Professor Ray WU

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Science china(Life Sciences) 2010年第4期53卷 531-532页

作者： HUANG Bing-Ren national laboratory of medical molecular biology, department of biochemistry and molecular biology, peking union medical college & Chinese Academy of medical Sciences, beijing 100005, china national laboratory of medical molecular biology Department of Biochemistry and Molecular Biology Peking Union Medical College & Chinese Academy of Medical Sciences

During the Chinese New Year holidays in 2008, Professor Zhang Nai-Heng, former chair of the department of biochemistry, beijing medical college, informed me of the sad news that Professor Ray Wu had passed away in Ith... 详细信息

关键词： In Memoriam very Professor Ray WU DNA

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Stem cell research:from molecular physiology to therapeutic applications

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Science china(Life Sciences) 2009年第7期52卷 597-598页

作者： Chengyu Jiang department of biochemistry and molecular biology,peking union medical college,Chinese Academy of medical Sciences,No.5 DongDanSanTiao,beijing,100005,P.R.china department of biochemistry and molecular biology Peking Union Medical College Chinese Academy of Medical Sciences Beijing P. R. China

Stem cell research promises remedies to many devastating diseases that are currently incurable, ranging from diabetes and Parkinson’s disease to paralysis. Totipotent embryonic stem cells have great potential for gen... 详细信息

关键词： cell Stem cell research

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Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line

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Acta Pharmacologica Sinica 2003年第12期24卷 61-66页

作者： WANG Lei, ZHANG Gui-Mei, FENG Zuo-Hua department of biochemistry and molecular biology, Tongji medical college Huazhong University of Science and Technology, Wuhan 430030, china department of biochemistry and molecular biology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

AIM: To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in downregulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line. METHODS: The expression of survivin mRNA was measured by RTPCR and the expression of survivin protein was measured by Western blot. Caspase-3 activity was determined by Phar Mingen colorimetric assay kit. Apoptosis was assessed by flow cytometry. The chemosensitivity of HL-60/ADR cells to adriamycin (ADR) was measured by MTT assay. RESULTS: pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, and induced apoptosis of HL-60/ADR cells in a time-dependent manner during 12-48 h. After transient transfection with pcDNA3.1/survivin for 48 h, survivin mRNA decreased by 67％, survivin protein decreased by 57％, caspase-3 activity increased 4.37 times, and the apoptosis rate increased by 4.41％ compared wi

关键词： antisense RNA doxorubicin apoptosis survivin caspase-3 HL-60 cells multiple drug resistance

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Structure and Function of the NS1 Protein of Influenza A Virus

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 155-162页

作者： Dongzi LIN~1 Jingfang LAN~2 Zhizhen ZHANG~1* 1 department of biochemistry and molecular biology,Guangdong medical college,Zhanjiang 524023,china 2 department of Clinical laboratory,Hejin People's Hospital,Hejin 043300,china department of biochemistry and molecular biology Guangdong Medical CollegeZhanjiang 524023 China department of Clinical laboratory Hejin People's HospitalHejin 043300 China

The avian influenza A virus currently prevailing in Asia causes fatal pneumonia and multipleorgan failure in birds and *** intensive research,understanding of the characteristics of influenzaA virus that determine its virulence is ***1A protein,a non-structural protein of influenza Avirus,was reported to contribute to its pathogenicity and ***1A protein is a multifunctionalprotein that plays a significant role in resisting the host antiviral response during the influenza *** briefly outlines the current knowledge on the structure and function of the NS1A protein.

关键词： influenza A virus NS1A protein RNA-binding domain effector domain dimer resistance antiviral response

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Overexpression of heat-shock protein 20 in rat heart myogenic cells confers protection against simulated ischemia/reperfusion injury

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Acta Pharmacologica Sinica 2005年第9期26卷 1076-1080页

作者： Yan-hui ZHU Xian WANG~2 department of Physiology and Pathophysiology,Basic medical college,Key laboratory of molecular Cardiovascular biology,Ministry of Education,peking University,beijing 100083,china department of Physiology and Pathophysiology Basic Medical College Key Laboratory of Molecular Cardiovascular Biology Ministry of Education Peking University Beijing China

Aim:To explore whether overexpression of the small heat shock protein HSP20 inrat cardiomyocytes protects against simulated ischemia/reperfusion(SI/R)***:Recombinant adenovirus expressing HSP20 was used to infect rat H9c2cardiomyocytes at high efficiency,as assessed by green fluorescent protein.H9c2cells were subjected to SI/R stress;survival was estimated through assessment oflactate dehydrogenase and cell apoptosis through caspase-3 ***:Overexpression of HSP20 decreased lactate dehydrogenase release by 21.5% andcaspase-3 activity by 58.8%.Pretreatment with the protein kinase C inhibitor Ro-31-8220(0.1 μmol/L)for 30 min before SI/R canceled the protective effect of *** selective mitochondrial K+awP channel inhibitor 5-hydroxydecanoate(100 μmol/L)had a similar ***,the non-selective K~+ATPchannel inhibitorglibenclamide(100 μmol/L)had no significant ***:These dataindicate that the protective effect of HSP20 in vitro is primarily due to reducednecrotic and apoptotic death of cardiomyocytes,possibly via the protein kinaseC/mitochondrial K~+ATP pathway.

关键词： reperfusion injury adenovirus apoptosis necrosis heat-shock proteins

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Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization

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Journal of Genetics and Genomics 2008年第1期35卷 11-16页

作者： Yiling Yang a, b, Jiayou Chu a, Yupeng Wu b, Manli Luo b, Xin Xu b, Yaling Han b, Yan Cai b, Qimin Zhanb, Mingrong Wang b, a department of Genetics, Institute of medical biology, peking union medical college and Chinese Academy of medical Sciences, Kunming 650118, china b State Key laboratory of molecular Oncology, Cancer Institute (Hospital), peking union medical college and Chinese Academy of medical Sciences, beijing 100021, china department of Genetics Institute of Medical Biology Peking Union Medical College and Chinese Academy of Medical Sciences Kunming 650118 China State Key laboratory of molecular Oncology Cancer Institute (Hospital) Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100021 China

Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization (M-FISH) for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermedi- ate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chro- mosomes in the whole genome of human tumors.

关键词： multicolor fluorescence in situ hybridization KYSE 410-4 karyotype esophageal squamous cell carcinoma

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Comparison of differential gene expression profiles in human esophageal squamous carcinoma EC8712 cells before and after arsenic trioxide (As_2O_3) treatment

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Chinese Science Bulletin 1999年第17期44卷 1581-1587页

作者： XIE Dongxu, DING Fang, WANG Xiuqin, LIU Zhihua, LUO Aiping and WU Minnational laboratory of molecular Oncology, department of Cell biology, Cancer Institute , Chinese Academy of medical Sciences and peking union medical college , beijing 100021, china national laboratory of molecular Oncology Department of Cell Biology Cancer Institute Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

To elucidate molecular mechanisms of As2O3 induced apoptosis of cancer cells in vitro, Atlas human cDNA expression analysis has been used for the profile of the known genes expressed in the human esophageal squamous carcinoma cells before and after treated by As2O3. On treating EC8712 cells with As2O3, most of the oncogenes have been down-regulated, while some tumor suppressor genes, such as DCC, up-regulated. Cyclin H decreases,

关键词： arsenic trioxide esophageal squamous carcinoma cell apoptosis gene expression.

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Depolarization-induced PKC activities suppress myogenin-driven AChR γ gene promoter activity

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Chinese Science Bulletin 1997年第15期42卷 1313-1317页

作者： DANG Xitong and JIA Hongtidepartment of biochemistry and molecular biology, beijing medical University, beijing 100083, china department of biochemistry and molecular biology Beijing Medical University Beijing China

NICOTINIC acetylcholine receptor （AChR） is a pentameric complex （α2βγ/εδ）, which is com-posed of four different subunits; its expression is regulated by neurally evoked electrical activi-ty. Before innervation in skeletal muscle during embryonic stage, AChR is expressed on thefiber surface; after synaptic formation, the expression of AChR is restricted to the motor end-plate. Denervation of the neonate or blockage of the electrical activity by sodium channel in-

关键词： protein kinase C myogenin AChR γ-promoter depolarization excitation-transcription coupling.

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Gene transfer of heat-shock protein 20 protects against ischemia/reperfusion injury in rat hearts

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Acta Pharmacologica Sinica 2005年第10期26卷 1193-1200页

作者： Yan-hui ZHU, Tie-min MA, Xian WANG~2 department of Physiology and Pathophysiology, Basic medical college, Key laboratory of molecular Cardiovascular biology, Ministry of Education, peking University, beijing 100083, china department of Physiology and Pathophysiology Basic Medical College Key Laboratory of Molecular Cardiovascular Biology Ministry of Education Peking University Beijing China

Aim: To explore whether overexpression of HSP20 in the myocardium could protect against ischemia/reperfusion injury in rats. Methods: Rat hearts were injected with vector, recombinant adenovirus encoding green fluorescent protein(***) or recombinant adenovirus encoding wild-type HSP20 (***20) in the left ventricle. Four days later, hearts were removed and expression of HSP20was measured in the left ventricle. Subsets of animals in the vector-, ***-, ***20-treated groups were subjected to 20-rain ischemia and 120-rainreperfusion. Myocardial injury was evaluated by infarct size and level of serumcardiac troponin T and creatine phosphokinase. Apoptosis of cardiomyocyteswas determined by TUNEL staining. Cardiac function was evaluated by hemodyamic indexes. Results: Infarct size and serum cardiac troponin T and creatinephosphokinase levels were significantly reduced in ***20-treated hearts compared with vector-and ***-treated hearts. The ratio of TUNEL-positivecardiomyocytes to total number of cardiomyocytes in the ***20 group wassignificantly reduced as compared with the vector and *** groups. Left ventricular end systolic pressure, and maximal rate of pressure increase (+dp/dtmax)and decrease (-dp/dtmin) values were increased significantly, while left ventricularend diastolic pressure was decreased significantly in ***20-treated heartscompared with vector- and ***-treated hearts. Conclusion: These data indicate that the cardioprotective effects of HSP20 may contribute to the reduction ofmyocardial necrosis and apoptosis in ischemia/reperfusion injury in rats.

关键词： reperfusion injury adenovirus apoptosis myocardial infarction heat-shock proteins

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