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evaluation of Three Borate-Bioactive Glass Compositions for Antibacterial Applications

Advances in Microbiology 2021年第11期11卷 646-656页

作者： David Westenberg Ravi Viswanathan Deana L. Kadyk Suzanne Hibbs Jonathan Kopel delbert day Biological Sciences Missouri University of Science and Technology Rolla MO USA Chemistry Missouri University of Science and Technology Rolla MO USA Material Science and engineering Missouri University of Science and Technology Rolla MO USA

The prevalence of cases of antibiotic resistant infections and the limited number of new antibiotics call for new strategies to prevent bacterial infections. In this study, we evaluate the effectiveness of three different compositions of bioactive glass, doped with silver as an antimicrobial agent for potential applications in preventing bacterial infections. The different glasses were formulated to dissolve and release silver at different rates enabling their use in different applications either individually or in combination to achieve a desired effect. Silver oxide (Ag2O) was incorporated into various glass formulations and antibacterial effectiveness was measured using radius of inhibition. each silver-doped glass effectively inhibited the growth of escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa with radius of inhibition correlating directly with the dissolution rate of the glass—the faster dissolving glass producing the larger radius of inhibition. Suspending particles of the silver containing glass in phosphate buffered saline or fetal calf serum, slowed the dissolution of each glass and extended the effectiveness of the silver containing glass over time (based on size of the radius of inhibition) compared to water. Potential applications of the powdered glass formulations would be to incorporate glasses with different properties into coatings or cosmetics. To investigate the feasibility of these applications, the silver-glass powder was incorporated into polycaprolactone polymer to determine the antibacterial properties of the imbedded glass powder. Based on this investigation, the silver glass formulations reported herein have potential applications in the medical device, dental, pharmaceutical and cosmetic industries.

关键词： Glass Antibacterial Silver Borate

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Muscle amino acid profiles of eleven species of aquacultured animals and their potential value in feed formulation

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Aquaculture and Fisheries 2024年第4期9卷 642-652页

作者： ewen McLean Kelly B.Alfrey delbert M.Gatlin III T.Gibson Gaylord Frederick T.Barrows Aqua Cognoscenti LLC 479 Henslowe LaneWest ColumbiaSC29170USA Anthropocene Institute 855 El Camino RealSte 13A N399Palo AltoCA94301USA Texas A&M University Department of Ecology and Conservation BiologyCollege StationTX77843USA US Fish and Wildlife Service Bozeman Fish Technology Center4050 Bridger Canyon RoadBozemanMT59715USA Aquatic Feed Technologies LLC 48 West Plaza del LagoIslamoradaFL33036USA

The complete quantitative essential amino acid(eAA)requirements of aquacultured animals are largely unknown except for a handful of *** is problematic because formulation of least-cost diets for target animals demands precise knowledge of eAA *** way of approximating eAA requirements is to use correlations between whole-body and/or muscle eAA profiles using essential to total eAA ratios(A/e ratios).This method requires a reference,quantified eAA requirement,usually lysine(Lys).To systematically evaluate the A/e ratio method,muscle AA profiles were measured in 10 species of teleost and whiteleg *** fish,Lys represented the dominant muscle eAA measured,ranging between 7.06 and 9.58 g/100 g protein,whereas,in shrimp,arginine(Arg)was quantified as the principal *** non-eAA,glutamate(Glu)and glutamine were consistently the NeAA recorded at highest levels whereas,in shrimp,Glu+Gln was highest followed by aspartate(Asp)plus asparagine(Asn).except for Arg,which was twice that of fish(Peleg shrimp expressed similar muscle A/e *** of relationship between muscle eAA levels and known requirements were all strongly and positively correlated for non-salmonid *** using Lys as the reference eAA there were few semblances between measured and estimated eAA requirements.

关键词： Aquafeed A/e ratio eAA requirement Ideal protein Muscle Fishmeal replacement

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嗜水气单胞菌外膜蛋白ompA基因的原核表达及免疫保护性研究

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淡水渔业 2015年第5期45卷 3-10,28页

作者：郑宗林郑曙明 delbert M. Gatlin Ⅲ 汪开毓水产动物繁育和健康养殖研究中心西南大学荣昌校区重庆荣昌402460 Department of Wildlife and Fisheries Sciences and Faculty of Nutrition Texas A&M University SystemCollege StationTexas 77840-2258USA 四川农业大学动物医学院成都625014

为评价嗜水气单胞菌(Aeromonas hydrophila,Ah)PDK06株外膜蛋白A(omp A)的原核表达产物是否具有抗原性及其对斑点叉尾(Ictalurus punctatus)的免疫保护力,根据Gen Bank上嗜水气单胞菌外膜蛋白omp A基因序列(Gen Bank登录号:AF146597)设... 详细信息

为评价嗜水气单胞菌(Aeromonas hydrophila,Ah)PDK06株外膜蛋白A(omp A)的原核表达产物是否具有抗原性及其对斑点叉尾(Ictalurus punctatus)的免疫保护力,根据Gen Bank上嗜水气单胞菌外膜蛋白omp A基因序列(Gen Bank登录号:AF146597)设计去信号肽引物,扩增获得一段约1 000 bp的序列,经鉴定正确后,构建重组表达质粒p eT-32a(+)-pomp A;然后将该质粒转入BL21(De3)后用IPTG诱导表达,经纯化后得到pomp A重组蛋白;最后将pomp Aomp A蛋白与弗氏不完全佐剂(FIA)混合免疫斑点叉尾,评价其免疫效果。结果显示:克隆的嗜水气单胞菌pomp A基因全长为960 bp,编码319个氨基酸,融合表达的重组蛋白相对分子质量约为53,670 k Da;FIA能够提高斑点叉尾的溶菌酶活性、ACH50活性、头肾巨噬细胞呼吸暴发活性;pomp A组抗体水平在第4周达到峰值,FIA免疫组抗体水平在第5周达到峰值,且FIA能够显著提高斑点叉尾的抗体水平;攻毒后各组累计死亡率分别为93.33%(PBS)、93.33%(FIA)、50.00%(pomp A)和13.33%(FIA+pomp A),pomp A和FIA+pomp A组相对保护率分别为46.43%和85.71%;免疫组靶器官中的细菌数量显著低于对照组,且FIA+pomp A细菌含量最低。说明pomp A可以作为一个亚单位疫苗候选疫苗,而FIA佐剂能较好的增强亚单位疫苗的免疫效果。

关键词：嗜水气单胞菌(Aeromonas hydrophila) omp A 亚单位疫苗斑点叉尾(Ictalurus punctatus) 免疫效果

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